Ultrastructural study of GERL in beige mouse alveolar macrophages.

Alveolar macrophages of the beige mouse mutant have a system of smooth-surfaced elements with the hallmarks of GERL. GERL also appears to produce residual bodies, and both organelles show cytochemically demonstrable acid phosphatase activity. When cells are exposed to colloidal silver, the tracer is endocytosed via pinocytic vacuoles to GERL.

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ISOZYMES OF ACID PHOSPHATASE IN NORMAL AND CALMETTE-GUÉRIN BACILLUS-INDUCED RABBIT ALVEOLAR MACROPHAGES

Journal of Experimental Medicine ◽

10.1084/jem.128.5.1031 ◽

1968 ◽

Vol 128 (5) ◽

pp. 1031-1048 ◽

Cited By ~ 39

Author(s):

S. G. Axline

Keyword(s):

Acid Phosphatase ◽

Phosphatase Activity ◽

Alveolar Macrophages ◽

Acid Phosphatase Activity ◽

Enzyme Inhibitors ◽

Freezing And Thawing ◽

Ph Optimum ◽

Lysosomal Fraction ◽

Repeated Freezing ◽

Major Acid

The acid phosphatase activity of normal alveolar and BCG-induced alveolar macrophages has been examined. Five electrophoretically distinct forms of acid phosphatase have been identified in both normal and BCG-induced macrophages. The acid phosphatases can be divided into two major categories. One category, containing four distinct forms, is readily solubilized after repeated freezing and thawing or mechanical disruption The second category, containing one form, is firmly bound to the lysosomal membrane and can be solubilized by treatment of the lysosomal fraction with Triton X-100. The Triton-extractable acid phosphatase and the predominant aqueous soluble acid phosphatase have been shown to differ in the degree of membrane binding, in solubility, in net charge, and in molecular weight. The two pre-dominant phosphatases possess identical pH optimum and do not differ in response to enzyme inhibitors. BCG stimulation has been shown to result in a nearly twofold increase in acid phosphatase activity. A nearly proportionate increase in the major acid phosphatase forms has been observed.

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An Ultrastructural Study of the Localization of Acid Phosphatase Activity in the Untreated and Vitamin a Acid Treated Keratoacanthoma

Journal of Investigative Dermatology ◽

10.1111/1523-1747.ep12257146 ◽

1970 ◽

Vol 54 (2) ◽

pp. 126-130 ◽

Cited By ~ 10

Author(s):

Lawrence Prutkin ◽

Bruce Bogart

Keyword(s):

Acid Phosphatase ◽

Vitamin A ◽

Phosphatase Activity ◽

Ultrastructural Study ◽

Acid Phosphatase Activity ◽

Vitamin A Acid

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Erythrophagocytosis in the Liver in Hemolytic Anemias

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100100664 ◽

1968 ◽

Vol 26 ◽

pp. 184-185

Author(s):

O. T. Minick ◽

E. Orfei ◽

F. Volini ◽

G. Kent

Keyword(s):

Acid Phosphatase ◽

Oxidative Damage ◽

Phosphatase Activity ◽

Kupffer Cells ◽

Acid Phosphatase Activity ◽

Common Type ◽

Red Cell ◽

Cell Fragments ◽

Reticulo Endothelial System ◽

Important Site

Hemolytic anemias were produced in rats by administering phenylhydrazine or anti-erythrocytic (rooster) serum, the latter having agglutinin and hemolysin titers exceeding 1:1000.Following administration of phenylhydrazine, the erythrocytes undergo oxidative damage and are removed from the circulation by the cells of the reticulo-endothelial system, predominantly by the spleen. With increasing dosage or if animals are splenectomized, the Kupffer cells become an important site of sequestration and are greatly hypertrophied. Whole red cells are the most common type engulfed; they are broken down in digestive vacuoles, as shown by the presence of acid phosphatase activity (Fig. 1). Heinz body material and membranes persist longer than native hemoglobin. With larger doses of phenylhydrazine, erythrocytes undergo intravascular fragmentation, and the particles phagocytized are now mainly red cell fragments of varying sizes (Fig. 2).

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