scholarly journals Fibronectin in the developing sea urchin embryo.

1980 ◽  
Vol 87 (1) ◽  
pp. 309-313 ◽  
Author(s):  
E Spiegel ◽  
M Burger ◽  
M Spiegel
Keyword(s):  
Cell Adhesion ◽  
Early Development ◽  
Sea Urchin ◽  
Immunofluorescence Staining ◽  
Cell Surfaces ◽  
Fluorescence Pattern ◽  
Sea Urchin Embryos ◽  
Sea Urchin Embryo

The presence of fibronectin in developing sea urchin embryos was studied uing immunofluorescence staining. The fluorescence pattern indicates that fibronectin is found on the cell surfaces and between cells in the blastula and gastrula stages, indicating that it plays a role in cell adhesion. Its presence on invaginating cells also suggests its involvement in morphogenesis during early development.

Synthesis of nuclear and cytoplasmic proteins in the early development of fishes and echinoderms

Development ◽  
1971 ◽  
Vol 26 (3) ◽  
pp. 611-622
Author(s):  
Maya R. Krigsgaber ◽  
Alla A. Kostomarova ◽  
Tamara A. Terekhova ◽  
Tatiana A. Burakova
Keyword(s):  
Amino Acids ◽  
Early Development ◽  
Sea Urchin ◽  
Nuclear Volume ◽  
Sea Urchin Embryos ◽  
Cytoplasmic Proteins ◽  
Specific Activities ◽  
Strongylocentrotus Nudus ◽  
Silver Grains ◽  
Misgurnus Fossilis

Synthesis of nuclear and cytoplasmic proteins was studied biochemically and autoradiographically in early loach (Misgurnus fossilis) and sea-urchin (Strongylocentrotus nudus) embryos. After incubation with [14C]amino acids for 5–120 min the ratio of the specific activities of nuclear, mitochondrial and 12000 g supernatant proteins was shown to be equal approximately to 6:1:2 in loach embryos and to 8:4:3 in sea-urchin embryos independently of the duration of labelling. After incubation with [3H]amino acids the number of silver grains per unit section was on the average 2·4 times higher for nuclei than it was for cytoplasm at mid-blastula and mid-gastrula stages. At the mid-gastrula the vegeto-animal gradient of protein synthesis was found. A higher level of the synthesis of nuclear proteins as compared with that of cytoplasmic proteins appears to be related to an increase in the nuclear volume and the nucleo-cytoplasmic ratio during the early development of the loach and sea-urchin embryos.

The Apical Lamina and its Role in Cell Adhesion in Sea Urchin Embryos

1998 ◽  
Vol 5 (2) ◽  
pp. 97-108 ◽  
Author(s):  
Robert D. Burke ◽  
Mahinder Lail ◽  
Yoko Nakajima
Keyword(s):  
Cell Adhesion ◽  
Sea Urchin ◽  
Sea Urchin Embryos

Cell junctions during the early development of the sea urchin embryo (Paracentrotus lividus)

1987 ◽  
Vol 20 (2-3) ◽  
pp. 137-146 ◽  
Author(s):  
P. Andreuccetti ◽  
M.R. Barone Lumaga ◽  
G. Cafiero ◽  
S. Filosa ◽  
E. Parisi
Keyword(s):  
Early Development ◽  
Sea Urchin ◽  
Paracentrotus Lividus ◽  
Cell Junctions ◽  
Sea Urchin Embryo

scholarly journals H+/K+ ion pump enhances cytoskeletal polarity to drive gastrulation in sea urchin embryo

2021 ◽  
Author(s):  
Kaichi Watanabe ◽  
Yuhei Yasui ◽  
Yuta Kurose ◽  
Masashi Fujii ◽  
Takashi Yamamoto ◽  
...  
Keyword(s):  
Sea Urchin ◽  
Actin Polymerization ◽  
Driving Forces ◽  
Ph Control ◽  
Structural Features ◽  
Normal Embryo ◽  
Ion Pump ◽  
Sea Urchin Embryos ◽  
Molecular Events ◽  
Sea Urchin Embryo

Gastrulation is a universal process in the morphogenesis of many animal embryos. In sea urchin embryos, it involves the invagination of single-layered vegetal plate into blastocoel. Although morphological and molecular events have been well studied for gastrulation, the mechanical driving forces and their regulatory mechanism underlying the gastrulation is not fully understood. In this study, structural features and cytoskeletal distributions were studied in sea urchin embryo using an "exogastrulation" model induced by inhibiting the H+/K+ ion pump with omeprazole. The vegetal pole sides of the exogastrulating embryos had reduced roundness indices, intracellular pH polarization, and intracellular F-actin polarization at the pre-early gastrulation compared with the normal embryo. Gastrulation stopped when F-actin polymerization or degradation was inhibited by RhoA or YAP1 knockout, although pH distributions were independent of such a knockout. A mathematical model of sea urchin embryos at the early gastrulation reproduced the shapes of both normal and exogastrulating embryos using cell-dependent cytoskeletal features based on F-actin and pH distributions. Thus, gastrulation required appropriate cell position-dependent intracellular F-actin distributions regulated by the H+/K+ ion pump through pH control.

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