scholarly journals IMMUNOLOGICAL UNRESPONSIVENESS TO SENSITIZATION WITH SIMPLE CHEMICAL COMPOUNDS

1963 ◽  
Vol 118 (6) ◽  
pp. 1021-1035 ◽  
Author(s):  
Jack R. Battisto ◽  
Merrill W. Chase
Keyword(s):  
Specific Antibody ◽  
Blood Cells ◽  
Guinea Pigs ◽  
White Blood Cells ◽  
Chemical Compounds ◽  
Lymphoid Cells ◽  
Delayed Type Hypersensitivity ◽  
Simple Chemical ◽  
Picryl Chloride

Guinea pigs fed picryl chloride to induce specific immunologic unresponsiveness cleared small amounts of venously infused antipicryl antibody at a rate equal to that of normal guinea pigs. Catabolism of passively administered picryl-specific antibody did not alter the unresponsive state of picryl chloride-fed guinea pigs or the responsive state of normal guinea pigs. Lymphoid cells of picryl chloride immunized guinea pigs produced equal amounts of picryl-specific antibody in picryl chloride-fed and normal animals. Allergen-fed guinea pigs remained unresponsive to attempted sensitization with the allergen in excess of 10 months after the final feeding, though some became feebly sensitive between 9 and 11 months. Second attempts to make unresponsive animals hypersensitive were unsuccessful. White blood cells of guinea pigs unresponsive to picryl chloride were unable to transfer delayed-type hypersensitivity for picryl chloride to normal recipients yet readily transferred tuberculin hypersensitivity.

scholarly journals STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS

1937 ◽  
Vol 66 (3) ◽  
pp. 337-351 ◽  
Author(s):  
K. Landsteiner ◽  
M. W. Chase
Keyword(s):  
Contact Dermatitis ◽  
Strong Evidence ◽  
Guinea Pigs ◽  
Chemical Properties ◽  
Chemical Compounds ◽  
Protein Conjugates ◽  
Chemical Constitution ◽  
Simple Chemical ◽  
Picryl Chloride ◽  
Cutaneous Administration

It has been shown that by the cutaneous administration of simple chemical compounds in small quantities—2:4:6 trinitrochlorobenzene (picryl chloride) and 2:4 dinitrochlorobenzene, the latter a typical incitant of contact dermatitis in man—it is possible to induce true anaphylactic sensitization in guinea pigs, demonstrable by the intravenous injection of protein conjugates and by the Dale technique, using isolated uterine horns. This furnishes strong evidence for the formation of antigenic conjugates following application of substances of simple chemical constitution. Since the anaphylactic state is induced by the same method of administration that gives rise to cutaneous sensitivity, the assumption would appear justified, when one takes into account the chemical properties of the inciting substances, that the formation of conjugated antigens offers an explanation for the skin effects also. In the experiments with picryl chloride, anaphylactic antibodies, and occasionally precipitins, have been demonstrated. The differences between the cutaneous and anaphylactic types of sensitivity are discussed.

scholarly journals STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS

1941 ◽  
Vol 73 (3) ◽  
pp. 431-438 ◽  
Author(s):  
K. Landsteiner ◽  
M. W. Chase
Keyword(s):  
Guinea Pigs ◽  
Intraperitoneal Injection ◽  
Chemical Compounds ◽  
Skin Sensitization ◽  
Experimental Conditions ◽  
Contact Type ◽  
Simple Chemical ◽  
Picryl Chloride ◽  
Typical Skin

Experiments with guinea pigs are described which show that under special experimental conditions the intraperitoneal injection of conjugates made with homologous erythrocyte stromata leads to typical skin sensitization of the contact type towards the respective simple chemicals, namely picryl chloride or 2,4-dinitrofluorobenzene. Therefore such sensitivity can be brought about not only by low molecular chemical compounds but by a material which must be regarded as a typical antigen.

scholarly journals STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS

1935 ◽  
Vol 61 (5) ◽  
pp. 643-656 ◽  
Author(s):  
K. Landsteiner ◽  
John Jacobs
Keyword(s):  
Aromatic Compounds ◽  
Guinea Pigs ◽  
Chemical Compounds ◽  
Human Beings ◽  
Factory Workers ◽  
Positive Effects ◽  
Negative Results ◽  
Simple Chemical ◽  
Picryl Chloride

Experiments on the sensitization of guinea pigs with simple chemical compounds are described. Positive effects were obtained by the administration of small quantities, namely fractions of milligrams, with 1:2:4 chlorodinitrobenzene, p-nitrosodimethylaniline, 1:2:4 trinitrobenzene, picryl chloride, four dichlorodinitrobenzenes, and a number of other aromatic compounds. Several substances chemically similar to those enumerated gave negative results. The first named compound is known to produce hypersensitiveness in human beings, a large number of cases having been observed in factory workers. The mechanism of these effects is discussed.

scholarly journals INDUCED UNRESPONSIVENESS TO SIMPLE ALLERGENIC CHEMICALS

1965 ◽  
Vol 121 (4) ◽  
pp. 591-606 ◽  
Author(s):  
Jack R. Battisto ◽  
Merrill W. Chase
Keyword(s):  
Guinea Pig ◽  
Specific Antibody ◽  
Guinea Pigs ◽  
Subsequent Development ◽  
Intradermal Injection ◽  
Contact Hypersensitivity ◽  
Delayed Type Hypersensitivity ◽  
Contact Type ◽  
Specific Contact ◽  
Picryl Chloride

Normal guinea pigs fed chemical haptens develop a specific state of unresponsiveness, inhibiting subsequent development of dermal sensitization with the same hapten and modifying profoundly the synthesis of anaphylactic antibody in response to hapten conjugated to guinea pig proteins. The degree of unresponsiveness has been tested by exposing hapten-fed animals to intense haptenic stimulation. Animals of groups that were demonstrably unresponsive to picryl chloride could be made to form hapten-specific antibody by injecting picrylated bovine gamma globulin. Specific anaphylactic-type antibodies, presumably 7S γ1, were synthesized, and in animals given PBGG adsorbed to alumina there arose a measurable concentration of precipitating antibody, presumably 7S γ2, perhaps slightly earlier than in similarly treated control animals. Attempts to impose contact-type reactivity on such unresponsive animals met with limited success. Injection of picrylated guinea pig erythrocyte stromata in a complete Freund's adjuvant, with subsequent applications of picryl chloride to the dermis, led to definite contact sensitivity to 0.3 per cent picryl chloride, whereas parallel treatment of normal control animals induced sensitivity to 0.006 or 0.002 per cent. By this double method of stimulation, hapten-fed animals did not advance in sensitivity by reason of the secondary dermal applications of the simple chemical, whereas control animals developed increasingly higher sensitivity by these contacts in what appeared to be a stepwise manner. Picryl chloride-fed guinea pigs injected intradermally with picryl chloride either after or before forming picryl-specific circulating antibody still remained unable to develop picryl-specific contact hypersensitivity. Control animals synthesizing picryl-specific antibody subsequently responded to intradermal injection of picryl chloride with contact-type sensitivity. Interpretations of these results are discussed and the view is presented that delayed-type hypersensitivity and circulating antibodies of the varieties measured here are formed independently of each other.

scholarly journals STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS

1947 ◽  
Vol 86 (6) ◽  
pp. 489-514 ◽  
Author(s):  
Merrill W. Chase
Keyword(s):  
Guinea Pigs ◽  
Chemical Compounds ◽  
Human Beings ◽  
Acyl Chlorides ◽  
Protein Conjugates ◽  
Simple Chemical ◽  
Picryl Chloride ◽  
Acid Anhydrides ◽  
Substantial Degree

Evidence is presented to show that guinea pigs actively sensitized to simple chemical compounds form serum antibodies capable of sensitizing the skin of normal guinea pigs. Skin sites prepared as for the Prausnitz-Küstner test develop immediate-type ("evanescent") reactions with erythema and edema, upon subsequent injection of the corresponding simple compounds or protein conjugates thereof, and give effects resembling transferred reaginic reactions as seen in human beings. The antibodies were obtainable after sensitization by acyl chlorides, acid anhydrides, and also substances of lesser reactivity, picryl chloride and 2:4 dinitrochlorobenzene, which are human allergens. Observations are reported on the specificity of the antibodies and on various details of the reaction. Like effects result when antiprotein immune sera and their corresponding antigens are employed for the test, making it highly probable that the antibodies secured after sensitization to drugs result from immunization by conjugates formed in vivo. The sera obtained after sensitization with simple chemical compounds readily confer passive anaphylaxis, and their capacity for sensitizing the skin declines gradually with progressive heating. It was observed that following a reaction of substantial degree in guinea pig skin the area involved does not fully recover for some days its capacity to react, the effect being a manifestation, it would seem, of what has been termed "non-specific antianaphylaxis."

Lead exposure affects inflammatory mediators, total and differential white blood cells in sensitized guinea pigs during and after sensitization

2013 ◽  
Vol 37 (3) ◽  
pp. 329-335 ◽  
Author(s):  
Tahereh Farkhondeh ◽  
Mohammad Hossein Boskabady ◽  
Mohammad Kazem Kohi ◽  
Goudarz Sadeghi-Hashjin ◽  
Mostafa Moin
Keyword(s):  
Lead Exposure ◽  
Inflammatory Mediators ◽  
Blood Cells ◽  
Guinea Pigs ◽  
White Blood Cells

scholarly journals STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS

1940 ◽  
Vol 71 (2) ◽  
pp. 237-245 ◽  
Author(s):  
K. Landsteiner ◽  
M. W. Chase
Keyword(s):  
Contact Dermatitis ◽  
Intraperitoneal Injection ◽  
Chemical Compounds ◽  
Route Of Administration ◽  
Necessary Condition ◽  
Skin Sensitization ◽  
Paraffin Oil ◽  
Simple Chemical ◽  
Picryl Chloride ◽  
Dermal Application

A method has been described by which sensitization to a simple chemical, picryl chloride (2:4:6 trinitrochlorobenzene), can be satisfactorily attained by means of intraperitoneal injection of the compound when killed tubercle bacilli suspended in paraffin oil were used as adjuvant. Sensitivity of the contact dermatitis type results therefrom. It follows that although skin sensitization of this type is most easily obtained by dermal application this route of administration is no necessary condition for such sensitivity.

scholarly journals STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS

1936 ◽  
Vol 64 (5) ◽  
pp. 717-721 ◽  
Author(s):  
K. Landsteiner ◽  
John Jacobs
Keyword(s):  
Intravenous Administration ◽  
Guinea Pigs ◽  
Anaphylactic Shock ◽  
Chemical Compounds ◽  
Simple Chemical

Experiments are described which show that with a given treatment guinea pigs can be sensitized to arsphenamine, so that a considerable percentage die in anaphylactic shock on intravenous administration of the substance.

scholarly journals INHERITANCE IN GUINEA PIGS OF THE SUSCEPTIBILITY TO SKIN SENSITIZATION WITH SIMPLE CHEMICAL COMPOUNDS

1941 ◽  
Vol 73 (6) ◽  
pp. 711-726 ◽  
Author(s):  
Merrill W. Chase
Keyword(s):  
Guinea Pigs ◽  
Simple Structure ◽  
Direct Evidence ◽  
Chemical Compounds ◽  
Skin Sensitization ◽  
Simple Chemical ◽  
Set Up

It has proved possible to set up lines of guinea pigs of significantly different susceptibilities towards a compound of simple structure, namely 2:4 dinitrochlorobenzene. This provides direct evidence that the type of sensitization under discussion is influenced by heredity.

The effect of lead exposure on tracheal responsiveness to methacholine and ovalbumin, total and differential white blood cells count, and serum levels of immunoglobulin E, histamine, and cytokines in guinea pigs

2013 ◽  
Vol 33 (3) ◽  
pp. 325-333 ◽  
Author(s):  
T Farkhondeh ◽  
MH Boskabady ◽  
S Jalali ◽  
G Bayrami
Keyword(s):  
Blood Cells ◽  
Guinea Pigs ◽  
Lead Acetate ◽  
Immunoglobulin E ◽  
White Blood Cells ◽  
Serum Levels ◽  
Control Group ◽  
Significant Difference ◽  
Ifn Γ ◽  
Pb Concentration

The effect of exposure to inhaled lead acetate in guinea pigs was evaluated. The present study comprised of five groups of guinea pigs including control (C), sensitized to ovalbumin (OA; S) and three groups exposed to 0.1, 0.2, and 0.4 M inhaled lead (Pb; n = 6 for each group). Tracheal responsiveness to methacholine and OA, total and differential white blood cells (WBCs) count in lung lavage, serum levels of cytokines (interferon γ (IFN-γ) and interleukin 4 (IL-4)), histamines, and immunoglobulin E (IgE), and Pb concentration in lung were measured. Tracheal responsiveness to methacholine, OA, total and differential WBC types as well as IL-4, IFN-γ, histamine, and IgE were significantly increased but IFN-γ/IL-4 were significantly decreased in sensitized animals as well as those exposed to high Pb concentrations when compared with the control group (from p < 0.05 to p < 0.001). In addition, there was not a significant difference in most measured values between animals exposed to high Pb concentration and group S. The Pb concentration in lung tissues of animals exposed to all three Pb concentrations was significantly higher than that of group C ( p < 0.001 for all cases).These results showed that inhaled lead acetate exposure can induce lung inflammatory changes similar to sensitized animals. Therefore, exposure to environmental Pb pollution may cause asthma-like changes.

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