Lead exposure affects inflammatory mediators, total and differential white blood cells in sensitized guinea pigs during and after sensitization

2013 ◽  
Vol 37 (3) ◽  
pp. 329-335 ◽  
Author(s):  
Tahereh Farkhondeh ◽  
Mohammad Hossein Boskabady ◽  
Mohammad Kazem Kohi ◽  
Goudarz Sadeghi-Hashjin ◽  
Mostafa Moin
Keyword(s):  
Lead Exposure ◽  
Inflammatory Mediators ◽  
Blood Cells ◽  
Guinea Pigs ◽  
White Blood Cells

scholarly journals IMMUNOLOGICAL UNRESPONSIVENESS TO SENSITIZATION WITH SIMPLE CHEMICAL COMPOUNDS

1963 ◽  
Vol 118 (6) ◽  
pp. 1021-1035 ◽  
Author(s):  
Jack R. Battisto ◽  
Merrill W. Chase
Keyword(s):  
Specific Antibody ◽  
Blood Cells ◽  
Guinea Pigs ◽  
White Blood Cells ◽  
Chemical Compounds ◽  
Lymphoid Cells ◽  
Delayed Type Hypersensitivity ◽  
Simple Chemical ◽  
Picryl Chloride

Guinea pigs fed picryl chloride to induce specific immunologic unresponsiveness cleared small amounts of venously infused antipicryl antibody at a rate equal to that of normal guinea pigs. Catabolism of passively administered picryl-specific antibody did not alter the unresponsive state of picryl chloride-fed guinea pigs or the responsive state of normal guinea pigs. Lymphoid cells of picryl chloride immunized guinea pigs produced equal amounts of picryl-specific antibody in picryl chloride-fed and normal animals. Allergen-fed guinea pigs remained unresponsive to attempted sensitization with the allergen in excess of 10 months after the final feeding, though some became feebly sensitive between 9 and 11 months. Second attempts to make unresponsive animals hypersensitive were unsuccessful. White blood cells of guinea pigs unresponsive to picryl chloride were unable to transfer delayed-type hypersensitivity for picryl chloride to normal recipients yet readily transferred tuberculin hypersensitivity.

The effect of lead exposure on tracheal responsiveness to methacholine and ovalbumin, total and differential white blood cells count, and serum levels of immunoglobulin E, histamine, and cytokines in guinea pigs

2013 ◽  
Vol 33 (3) ◽  
pp. 325-333 ◽  
Author(s):  
T Farkhondeh ◽  
MH Boskabady ◽  
S Jalali ◽  
G Bayrami
Keyword(s):  
Blood Cells ◽  
Guinea Pigs ◽  
Lead Acetate ◽  
Immunoglobulin E ◽  
White Blood Cells ◽  
Serum Levels ◽  
Control Group ◽  
Significant Difference ◽  
Ifn Γ ◽  
Pb Concentration

The effect of exposure to inhaled lead acetate in guinea pigs was evaluated. The present study comprised of five groups of guinea pigs including control (C), sensitized to ovalbumin (OA; S) and three groups exposed to 0.1, 0.2, and 0.4 M inhaled lead (Pb; n = 6 for each group). Tracheal responsiveness to methacholine and OA, total and differential white blood cells (WBCs) count in lung lavage, serum levels of cytokines (interferon γ (IFN-γ) and interleukin 4 (IL-4)), histamines, and immunoglobulin E (IgE), and Pb concentration in lung were measured. Tracheal responsiveness to methacholine, OA, total and differential WBC types as well as IL-4, IFN-γ, histamine, and IgE were significantly increased but IFN-γ/IL-4 were significantly decreased in sensitized animals as well as those exposed to high Pb concentrations when compared with the control group (from p < 0.05 to p < 0.001). In addition, there was not a significant difference in most measured values between animals exposed to high Pb concentration and group S. The Pb concentration in lung tissues of animals exposed to all three Pb concentrations was significantly higher than that of group C ( p < 0.001 for all cases).These results showed that inhaled lead acetate exposure can induce lung inflammatory changes similar to sensitized animals. Therefore, exposure to environmental Pb pollution may cause asthma-like changes.

scholarly journals A STUDY OF THE TOXIC PROPERTIES OF TUBERCULO-PROTEINS AND POLYSACCHARIDES

1931 ◽  
Vol 53 (1) ◽  
pp. 51-80 ◽  
Author(s):  
Florence R. Sabin ◽  
Franklin R. Miller ◽  
Charles A. Doan ◽  
Bruce K. Wiseman
Keyword(s):  
Blood Cells ◽  
Guinea Pigs ◽  
White Blood Cells ◽  
Temperature Reaction

The temperature reaction in tuberculous and normal guinea pigs and rabbits is elicited by the tuberculo-protein and probably not at all by the polysaccharides. The polysaccharides may have some killing power under certain conditions, but this is not as consistently related to dosage as in the case of the proteins. Both proteins and polysaccharides cause a change in the white blood cells when introduced by any route.

The effect of mycobacteriophage particles on cell-mediated immune reactions

1974 ◽  
Vol 20 (9) ◽  
pp. 1209-1218 ◽  
Author(s):  
E. Mankiewicz ◽  
V. Kurti ◽  
H. Adomonis
Keyword(s):  
Blood Cells ◽  
Guinea Pigs ◽  
White Blood Cells ◽  
Inhibitory Effect ◽  
Buffy Coat ◽  
Migration Inhibition ◽  
Immune Reactions ◽  
In Vitro Exposure ◽  
Spontaneous Migration

Suspensions of mycobacteriophages added to cultures of white blood cells (WBC's) reduce the phytohaemagglutinin (PHA) stimulated blast responses. Ultraviolet (UV) inactivation of the phages abolishes the inhibitory effect. Buffy coat cells from guinea pigs inoculated with mycobacteriophages and mice inoculated with these phages or with lysogenic mycobacteria show, without prior in vitro exposure to the antigen, significant reductions in the rate of their migration. This "spontaneous" migration inhibition is removed by prior treatment of the cells with trypsin or puromycin.

scholarly journals Effects of aspirin on the number of peripheral white blood cells and spleen eosinophils in guinea-pigs

2010 ◽  
Vol 60 (4) ◽  
pp. 355-362
Author(s):  
Drenka Turjacanin-Pantelic ◽  
I. Pantic ◽  
Senka Pantic ◽  
Elijana Garalejic ◽  
Dragana Jovic ◽  
...  
Keyword(s):  
Blood Cells ◽  
Guinea Pigs ◽  
White Blood Cells ◽  
Peripheral White Blood Cells

Experiments on Immunization of Guinea-Pigs with Normal and Leukemic Human White Blood Cells

1954 ◽  
Vol 12 (3) ◽  
pp. 188-192
Author(s):  
B. Landtman ◽  
Ruth Wegelius ◽  
Hilkka Tähkä
Keyword(s):  
Blood Cells ◽  
Guinea Pigs ◽  
White Blood Cells ◽  
Human White Blood Cells

scholarly journals Potential Anti-Inflammatory Properties Effect of Myrrh

2020 ◽  
Vol 9 (4) ◽  
pp. 1687-1694
Keyword(s):  
Inflammatory Mediators ◽  
Blood Cells ◽  
Inflammatory Diseases ◽  
White Blood Cells ◽  
Relevant Information ◽  
Bioactive Molecules ◽  
Clinical Effects ◽  
Anti Inflammatory ◽  
Knowledge Deficits ◽  
The Impact

The natural gum Myrrh, a resinous extrude of genus Commiphora species, has found several applications, especially in traditional medicine. Myrrh has been used for treating different diseases, including inflammatory diseases, in diverse communities across the world. Allopathic usage of Myrrh, however, is limited by knowledge deficits regarding pharmacological mechanisms underlying these clinical effects, in particular, its anti-inflammatory role. This review aims to provide up-to-date information on the effect of myrrh extracts as well as its bioactive compounds on the functions of white blood cells and inflammatory mediators. Relevant information about the impact of Myrrh on the functions of white blood cells and inflammatory mediators was collected from established scientific databases such as NCBI, Web of Science, and Google Scholar. A few books were also referred to as obtaining important information. Myrrh and its bioactive molecules have been shown to have potential effects on the functions of white blood cells and immunomodulatory activities. However, few studies have reported these effects. In-depth studies are necessary to determine the effect of Myrrh and its bioactive molecules on immune cells and inflammatory mediators.

scholarly journals In vivo Evaluation and in silico Prediction of the Toxicity of Drepanoalpha Hard capsules

2020 ◽  
Author(s):  
Benjamin Gbolo Zoawe ◽  
Jean-Paul Ngbolua Koto-te-Nyiwa ◽  
Damien Tshibangu Sha-Tshibey ◽  
Patrick Memvanga Bondo ◽  
Dorothee Tshilanda Dinangayi ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Lethal Dose ◽  
White Blood Cells ◽  
Normal Solution ◽  
Histopathological Study ◽  
Acute Administration ◽  
In Vivo Evaluation

This study was carried out in order to investigate the safety of Drepanoalpha hard hard capsules, a phytomedicine used for the management of sickle cell disease in the Democratic Republic of Congo by the acute and sub-acute administration in Guinea pigs. The hard capsules were dissolved in saline normal solution (NaCl 0.9 %). The animals were randomly selected, marked and divided into 2 groups of 5 animals each (3 males and 2 females) for acute toxicity and 4 groups of 3 animals each for sub-acute toxicity. Those received by gavage a single dose of 5000 mg/kg body weight (B.W.) of Drepanoalpha hard capsules for acute toxicity followed by 125 mg/Kg, 250 mg/Kg and 500 mg/Kg B.W. twice daily for 28 days for sub-acute toxicity and saline normal solution (NaCl 0.9 % solution as vehicle). Hematological, biochemical and histopathological analyses were performed and the behavior of the animals was observed after treatment. The results showed that 50 % of the lethal dose (LD50) is greater than 5000 mg/Kg B.W., and the relative weights of vital organs (kidney, liver, lungs, and heart) collected from Guinea pigs at the end of treatment on D14 (acute toxicity) and D28 (sub-acute toxicity) did not undergone significant changes (p>0.05). The results of haematological (Red Blood Cells, White Blood Cells, Haemoglobin, Haematocrit) and biochemical (ALT, AST, Albumin, Total Protein) tests did not show significant differences between the control and test groups at significance level (0.05 for acute toxicity), while the histopathological study revealed none damage to the various organs excised. In conclusion, the results confirm the safety of Drepanoalpha, as shown in previous studies with lyophilisate and decocate in rats and Guinea pigs. Keywords: Acute Toxicity, Sub-acute Toxicity, Hard Capsule, Drepanoalpha

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