scholarly journals STUDIES ON THE LYMPHOCYTOSIS INDUCED IN MICE BY BORDETELLA PERTUSSIS

1965 ◽  
Vol 121 (1) ◽  
pp. 49-68 ◽  
Author(s):  
Stephen I. Morse
Keyword(s):  
Phase I ◽  
Intravenous Injection ◽  
Pertussis Vaccine ◽  
Bordetella Pertussis ◽  
Subcutaneous Administration ◽  
Passive Immunization ◽  
Active Immunization ◽  
Lymphoid Organs ◽  
Circulating Lymphocytes ◽  
Intraperitoneal Inoculation

1. Intravenous injection into mice of phase I Bordetella pertussis vaccine resulted in a striking hyperleucocytosis with a predominating lymphocytosis. Intraperitoneal inoculation was less effective, and subcutaneous administration was inactive. 2. Active immunization prevented the hyperleucocytosis; passive immunization was less effective. 3. Reticuloendothelial blockage reduced the effect of the vaccine. 4. Extirpation of the spleen or thymus did not alter the leucocyte response. 5. Histologic studies suggested that the increase in circulating lymphocytes resulted from release of cells from lymphoid organs, including the thymus.

scholarly journals Protection against keratoconjunctivitis shigellosa induced by immunization with outer membrane proteins of Shigella spp

1980 ◽  
Vol 30 (2) ◽  
pp. 321-324
Author(s):  
G Adamus ◽  
M Mulczyk ◽  
D Witkowska ◽  
E Romanowska
Keyword(s):  
Membrane Proteins ◽  
Phase I ◽  
Outer Membrane ◽  
Guinea Pigs ◽  
Shigella Flexneri ◽  
Outer Membrane Proteins ◽  
Passive Immunization ◽  
Active Immunization ◽  
Immune Serum ◽  
Shigella Spp

Active immunization of guinea pigs and rabbits with outer membrane proteins (OMP) isolated from Shigella flexneri 3a and Shigella sonnei phase I protected the animals against keratoconjunctivitis shigellosa induced with the homologous or heterologous strain. Protection was also achieved in rabbits after passive immunization with anti-OMP immune serum. Active immunization with lipopolysaccharide of S. flexneri 3a did not protect rabbits against keratoconjunctivitis shigellosa.

scholarly journals Studies on Lymphocytes. XVIII. Mechanism of Lymphocytosis Induced by Supernatant Fluids of Bordetella pertussis Cultures

Blood ◽  
1971 ◽  
Vol 38 (1) ◽  
pp. 49-59 ◽  
Author(s):  
KANTI R. RAI ◽  
ARJUN D. CHANANA ◽  
EUGENE P. CRONKITE ◽  
DARREL D. JOEL ◽  
JERRY B. STEVENS
Keyword(s):  
Phase I ◽  
Liquid Medium ◽  
Intravenous Injection ◽  
Bordetella Pertussis ◽  
Thoracic Duct ◽  
Lymphocyte Count ◽  
Blood Lymphocyte ◽  
Splenic Vein ◽  
Duct Cell ◽  
Supernatant Fluid

Abstract Supernatant fluid from liquid medium cultures of phase I Bordetella pertussis organisms (pertussis supernatant) when given to calves and sheep by intravenous injection produces a marked lymphocytosis. Thoracic duct and splenic vein cannulation was performed in some animals to study the mechanism of production of this lymphocytosis. There was a transient increased mobilization of lymphocytes from the lymphoid organs as seen by the increase in output of lymphocytes from thoracic duct and splenic vein within a few minutes following administration of pertussis supernatant. This was followed by a decrease in the thoracic duct cell output as long as the blood lymphocyte count remained elevated, indicating an inhibition of recirculation of lymphocytes from blood to lymph. There was no evidence of increased new cell production.

scholarly journals B-cell responses after intranasal vaccination with the novel attenuated Bordetella pertussis vaccine strain BPZE1 in a randomized phase I clinical trial

Vaccine ◽  
2014 ◽  
Vol 32 (27) ◽  
pp. 3350-3356 ◽  
Author(s):  
Maja Jahnmatz ◽  
Sylvie Amu ◽  
Margaretha Ljungman ◽  
Lena Wehlin ◽  
Francesca Chiodi ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Phase I ◽  
B Cell ◽  
Vaccine Strain ◽  
Pertussis Vaccine ◽  
Bordetella Pertussis ◽  
Phase I Clinical Trial ◽  
The Novel ◽  
Cell Responses ◽  
B Cell Responses

scholarly journals THE EFFECT OF HYDROCORTISONE AND X-IRRADIATION ON THE LYMPHOCYTOSIS INDUCED BY BORDETELLA PERTUSSIS

1966 ◽  
Vol 123 (2) ◽  
pp. 283-298 ◽  
Author(s):  
Stephen I. Morse
Keyword(s):  
Pertussis Vaccine ◽  
Bordetella Pertussis ◽  
Lymphoid Tissues ◽  
Hydrocortisone Acetate ◽  
Circulating Cells ◽  
Circulating Lymphocytes ◽  
X Irradiation

1. In mice rendered lymphocytopenic by X-irradiation or hydrocortisone acetate, pertussis vaccine evoked both lymphocytosis and polymorphonuclear leukocytosis. 2. When mice with lymphocytosis induced by pertussis vaccine were X-irradiated, prompt and extensive destruction of circulating as well as tissue small lymphocytes occurred. The devitalized circulating cells were cleared from the blood primarily by the Kupffer's cells of the hepatic sinusoids. 3. Hydrocortisone acetate administered to mice with lymphocytosis did not cause acute lymphopenia nor was there any evidence of destruction of circulating small lymphocytes. However, destruction of these cells within lymphoid tissues was apparent. These observations suggested that adrenal cortical hormones are not "lymphocytolytic" with respect to circulating lymphocytes.

scholarly journals EPCT-13. CMV PP65 RNA-PULSED DENDRITIC CELL VACCINES FOR PEDIATRIC GLIOBLASTOMA AND MEDULLOBLASTOMA: PHASE I TRIAL RESULTS

2020 ◽  
Vol 22 (Supplement_3) ◽  
pp. iii306-iii306
Author(s):  
Daniel Landi ◽  
Gary Archer ◽  
Timothy Driscoll ◽  
Eric Lipp ◽  
Bridget Archambault ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Phase I ◽  
Malignant Glioma ◽  
Phase I Trial ◽  
Active Immunization ◽  
Monocyte Differentiation ◽  
Dendritic Cell Vaccines ◽  
Dc Vaccines ◽  
Subsequent Phase ◽  
Children And Young Adults

Abstract BACKGROUND Recurrent medulloblastoma and malignant glioma are lethal tumors that are virtually incurable. The cytomegalovirus (CMV) antigen pp65 is ubiquitously expressed on medulloblastoma and malignant glioma but not on healthy brain. We evaluated autologous CMV pp65 RNA-pulsed dendritic cell (DC) vaccines in children and young adults in a phase I trial. METHODS Circulating monocytes were harvested using leukapheresis, differentiated into DCs, matured, and pulsed with pp65 RNA using electroporation. DCs were packaged into vaccines (2x107DC/vaccine) and administered intradermally following tetanus-diphtheria toxoid site preconditioning every 2 weeks x3, then monthly. The primary objectives of the study were to establish the feasibility of generating at least 3 vaccines and safety. An exploratory objective was to evaluate the ability of vaccination to create and enhance patient pp65-specific T cell responses. RESULTS Eleven patients were enrolled with medulloblastoma (n=3) or glioblastoma (n=8). Ages ranged from 9–30 years old (mean 15.5y). Ten of 11 patients (91%) generated at least 3 vaccines (mean 6.2). Eight patients received at least 3 vaccines. To date, 4 patients have received all generated vaccines without progression, 4 patients have progressed, and 2 patients are still receiving vaccines. There have not been any severe adverse events probably or definitely related to vaccines. More mature data will be presented at ISPNO. CONCLUSIONS Leukapheresis and monocyte differentiation is a feasible strategy for generating adequate DCs for active immunization in children with malignant brain tumors. CMV pp65 RNA-pulsed DCs are well-tolerated and immunogenic. Efficacy endpoints will be evaluated in a subsequent phase II trial.

scholarly journals Diphtheria-tetanus-pertussis vaccine combined with Bordetella parapertussis vaccine

1962 ◽  
Vol 60 (3) ◽  
pp. 289-293 ◽  
Author(s):  
Neda Köhler-Kubelka
Keyword(s):  
Clinical Examination ◽  
Pertussis Vaccine ◽  
Bordetella Pertussis ◽  
Whooping Cough ◽  
Production Test ◽  
Post Vaccination ◽  
Bordetella Parapertussis ◽  
Cross Reactions ◽  
Combined Vaccine

Investigations carried out to ascertain the ability of various strains of Bordetella pertussis and B. parapertussis to produce agglutinins have shown that the agglutinin response is considerably greater with B. parapertussis.Children inoculated with a combined vaccine in which the parapertussis element contained B. parapertussis in only one-twelfth of the concentration of B. pertussis in the pertussis element showed agglutinins in their sera in titres well above 1:300 for both organisms. There were no cross-reactions and the serological responses were specific throughout. The vaccine used was the standard diphtheria-tetanus-pertussis (DTP) prophylactic to which had been added a vaccine prepared from recently isolated strains of B. parapertussis.Agglutinin titres of both whooping cough components with the combined vaccine were somewhat lower in mice than was the case when monovalent vaccines were used, but they were considered to be satisfactory.It is suggested that the agglutination production test in mice could be used for the assessment of protective power of B. parapertussis vaccines against infection.I wish to thank Dr Ikić, director of the Institute of Immunology, Zagreb, who enabled me to perform all these examinations, further to Dr B. Mravunac and Dr Z. Radanov for having carried out vaccination in children and for the clinical examination of post vaccination reactions.

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