scholarly journals The relationship between surface immunoglobulin isotype and immune function of murine B lymphocytes II. Surface immunoglobulin isotopes on unprimed B cells in the spleen.

1977 ◽  
Vol 145 (5) ◽  
pp. 1206-1215 ◽  
Author(s):  
I Zan-Bar ◽  
E S Vitetta ◽  
S Strober
Keyword(s):  
B Lymphocytes ◽  
Antibody Responses ◽  
Immunofluorescent Staining ◽  
Spleen Cells ◽  
Igg Antibody ◽  
Cell Sorter ◽  
Considerable Contribution ◽  
Immunoglobulin Isotype ◽  
Surface Immunoglobulin ◽  
The Relationship

We investigated the ability of IgM-, IgD-, and IgG-bearing cells from the spleens of unprimed (BALB/c x C57BL/Ka)F1 mice to restore the adoptive primary anti-BSA and anti-DNP antibody responses. Purified populations of isotype-specific cells were prepared by immunofluorescent staining and sorting on the fluorescence activated cell sorter. Bright or dull cells were transferred to irradiated syngeneic recipients which were challenged with DNP-BSA in complete Freund's adjuvant. Unfractionated spleen cells as well as IgM- and IgD-bearing cells restored the adoptive primary IgM and IgG antibody response. IgG-bearing cells restored a vigorous adoptive response which was all IgG (2-mercaptoethanol resistant). Depletion of IgG-bearing cells markedly increased the adoptive IgM response, and depletion of IgM-bearing cells markedly increased the IgG response. However, depletion of IgD-bearing cells resulted in a considerable reduction in the IgG response. The latter finding indicates that there is a subpopulation of IgD-bearing cells which express little or no surface IgM and which make a considerable contribution to the adoptive primary IgG response.

scholarly journals the relationship between surface immunoglobulin isotype and immune function of murine B lymphocytes. III. Expression of a single predominant isotype on primed and unprimed B cells

1978 ◽  
Vol 147 (5) ◽  
pp. 1374-1394 ◽  
Author(s):  
I Zan-Bar ◽  
ES Vitetta ◽  
F Assisi ◽  
S Strober
Keyword(s):  
B Cells ◽  
Bovine Serum Albumin ◽  
Antibody Response ◽  
B Lymphocytes ◽  
Primary Response ◽  
Spleen Cells ◽  
Cell Sorter ◽  
Immunoglobulin Isotype ◽  
Surface Immunoglobulin ◽  
The Relationship

We determined whether primed and unprimed B cells in the spleen of (BALB/c × C57BL/Ka)F(1) mice contain subpopulations that express a predominant surface Ig isotype. Spleen cells were stained for surface isotypes and sorted on the fluorescence-activated cell sorter (FACS) in order to obtain B cells bearing predominantly IgM (μp cells), IgD (δp cells), or IgG (γp cells). Each population was assayed for its capacity to restore the adoptive primary and secondary anti-bovine serum albumin (BSA) antibody response in irradiated syngeneic recipients. In addition, the adoptive response restored by isotype-predominant cells was compared to that restored by isotype- positive cells (B cells bearing a given surface isotype alone or in combination with others). The experimental results show that μp cells restore the adoptive primary and secondary IgM and IgG responses to BSA, and γP cells restore only the primary and secondary IgG response. Δp Cells restored the adoptive secondary IgG response, but failed to restore the adoptive primary response at the cell doses tested. ΓP Cells but not δp cells suppressed the IgM response of the μ(+) and δ(+) cells. The contribution of isotype-predominant cells to both the adoptive primary and secondary anti-BSA response was smaller than that of B cells bearing a combination of surface isotypes. Differences in the Ig isotype pattern expressed on the surface of primed and unprimed B cells are discussed.

scholarly journals The relationship between surface immunoglobulin isotype and immune function of murine B lymphocytes. I. Surface immunoglobulin isotypes on primed B cells in the spleen.

1977 ◽  
Vol 145 (5) ◽  
pp. 1188-1205 ◽  
Author(s):  
I Zan-Bar ◽  
S Strober ◽  
E S Vitetta
Keyword(s):  
Antibody Response ◽  
Serum Antibody ◽  
Immunofluorescent Staining ◽  
Spleen Cells ◽  
Igg Antibody ◽  
Surface Immunoglobulin ◽  
Immunoglobulin Isotypes ◽  
Cell Surface Staining ◽  
Surface Staining ◽  
The Relationship

We investigated the ability of IgM-, IgD-, and IgG-bearing cells from the spleens of (BALB/c x C57BL/Ka)F1 mice primed to dinitrophenyl-bovine serum albumin (DNP-BSA) to restore the adoptive secondary anti-BSA and anti-DNP antibody responses. A rabbit anti-mouse IgD antiserum was prepared and the specificity documented by radioimmunoprecipitation, and cell surface staining. Purified populations of IgM-, IgD-, and IgG-bearing cells were prepared by immunofluorescent staining with isotype-specific reagents, and sorting on the fluorescence activated cell sorter. Bright or dull cells were transferred to irradiated syngeneic recipients which were challenged with DNP-BSA in saline. Unfractionated spleen cells restored an adoptive secondary serum antibody response which was all IgG (2-mercaptoethanol resistant). Purified IgM- or IgD-bearing cells restored both the secondary IgM and IgG antibody response. IgG-bearing cells restored only the IgG response. In addition, the IgG-bearing cells appear to suppress the adoptive secondary IgM response, since depletion of IgG-bearing cells from transferred spleen cells results in a marked increase in the adoptive IgM response.

Surface immunoglobulin isotype, activation, and tolerance susceptibility of B lymphocytes of New Zealand black mice

1981 ◽  
Vol 63 (2) ◽  
pp. 385-399 ◽  
Author(s):  
B.L. Pike ◽  
J.E. Layton ◽  
N.R. Rose ◽  
G.J.V. Nossal
Keyword(s):  
New Zealand ◽  
B Lymphocytes ◽  
Immunoglobulin Isotype ◽  
Surface Immunoglobulin

scholarly journals SELECTIVE ROLES OF THYMUS-DERIVED LYMPHOCYTES IN THE ANTIBODY RESPONSE

1974 ◽  
Vol 140 (1) ◽  
pp. 239-252 ◽  
Author(s):  
Tomio Tada ◽  
Toshitada Takemori
Keyword(s):  
T Cells ◽  
B Cells ◽  
Antibody Response ◽  
Suppressive Effect ◽  
Antibody Responses ◽  
Cell Transfer ◽  
Spleen Cells ◽  
Igg Antibody ◽  
Igm Antibody

Passively transferred thymocytes and spleen cells from donors primed with keyhole limpet hemocyanin (KLH) exerted differential suppressive effect on IgM and IgG antibody responses of syngeneic recipients immunized with DNP-KLH depending primarily on the time when KLH-primed cells were transferred. This was demonstrated by the decrease in the numbers of DNP-specific direct and indirect PFC in the spleen of the recipients given KLH-primed cells at different times during primary and secondary immunization. Whereas the cell transfer simultaneously with or 2 days after the primary immunization produced only slight suppression of the peak IgM antibody response, it caused profound suppression of late IgM and IgG antibody responses. By contrast, the cell transfer 3 days after the immunization produced immediate suppression of the ongoing IgM antibody response resulting in its earlier termination, while being unable to prevent the induction of IgG antibody response. KLH-primed cells could moderately suppress the secondary anti-DNP antibody response, in which IgG antibody response was found to be slightly more sensitive than IgM antibody response to the suppressive influence of KLH-primed cells. The suppressive effect of the KLH-primed spleen cells was completely eliminated by the in vitro treatment of the cells with anti-θ and C before cell transfer, indicating that cells responsible for the suppression are, in fact, T cells. The suppression of DNP-specific antibody response by KLH-primed T cells was achieved only if the recipients were immunized with DNP-KLH but not with DNP-heterologous carrier, suggesting that direct interaction between T and B cells is necessary for the suppression of the antibody response. It is concluded that susceptibility of B cells to the specific suppressive influence of T cells is inherently different depending on the differentiation stage of B cells and on the immunoglobulin class they are destined to produce.

scholarly journals Regulation of murine B cells through surface immunoglobulin. I. Monoclonal anti-delta antibody that induces allotype-specific proliferation.

1980 ◽  
Vol 152 (5) ◽  
pp. 1135-1146 ◽  
Author(s):  
I M Zitron ◽  
B L Clevinger
Keyword(s):  
Monoclonal Antibody ◽  
B Cells ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Soluble Form ◽  
Spleen Cells ◽  
Surface Immunoglobulin

We describe the identification of a monoclonal antibody that recognizes a determinant on the delta chain of mice of the Iga, allotype groups. The monoclonal Ig in soluble form induces allotype-specific proliferation by splenic B lymphocytes from normal animals of these haplotypes. Spleen cells from mice bearing the X-linked defect of CBA/N mice fail to respond, although they bear the determinant. Proliferation is independent of T lymphocytes. The data indicate a direct triggering function for sIgD.

scholarly journals Isotype commitment in the in vivo immune responses. I. Antigen-dependent specific and polyclonal plaque-forming cell responses by B lymphocytes induced to extensive proliferation.

1982 ◽  
Vol 156 (3) ◽  
pp. 690-702 ◽  
Author(s):  
M Björklund ◽  
A Coutinho
Keyword(s):  
Immune Responses ◽  
B Lymphocytes ◽  
Antibody Responses ◽  
Regulatory Mechanisms ◽  
Transfer System ◽  
Spleen Cells ◽  
Serial Transfer ◽  
Specific Antibodies ◽  
Cell Responses

The random recombination and deletion hypothesis for the control of isotype commitment in antibody responses was directly tested in a serial transfer system in vivo. Normal or hyperimmune spleen cells were used in weekly serial transfers with antigen into irradiated recipients until clonal senescence was observed. Antigen-specific and -nonspecific plaque-forming cells of all isotypes were determined at each transfer time. No major changes in the isotypes of specific antibodies were observed for the whole life-span of the transferred cells (9-10 wk), and no indication was obtained for the accumulation of cells transcribing the most 3' members of the C-gene cluster with sustained proliferation. Rather, the dominant isotypes were found throughout the response to be IgG1, IgG2b, and IgG2a. The results imply isotype-specific regulatory mechanisms in the control of Ig class production. These appear to operate as well in the antigen-nonspecific component of the immune response.

scholarly journals SARS-CoV-2 IgG antibody responses in rt-PCR positive cases: first report from India

2020 ◽  
Author(s):  
Girish Chandra Dash ◽  
Debaprasad Parai ◽  
Hari Ram Choudhary ◽  
Annalisha Peter ◽  
Usha Kiran Rout ◽  
...  
Keyword(s):  
Antibody Response ◽  
Infected Individual ◽  
Antibody Responses ◽  
Rt Pcr ◽  
Serum Samples ◽  
Igg Antibody ◽  
Ct Value ◽  
Significant Difference ◽  
The Mean ◽  
The Relationship

AbstractThe SARS-CoV-2 antibody responses remain poorly understood and the clinical utility of serological testing is still unclear. As it is thought to confer some degree of immunity, this study is carried out to know the relationship between demographics and ct value of confirmed rt-PCR patients. A total of 384 serum samples were collected between 4-6 weeks after confirmed SARS-CoV-2 infection. IgG positivity was found to be 80.2% (95% CI, 76.2 – 84.2). The IgG positivity increased with the decrease in the ct value, with highest of 87.6% positivity in individuals with <20 ct value. The mean (± SD) ct value of IgG positives and og IgG negatives was 23.34 (± 6.09) and 26.72 (± 7.031) respectively. There was no significant difference found between the demographic characteristics such as age, sex, symptoms and antibody response. The current study is first of its kind wherein we have assessed the correlation of ct of RT-PCR with development of IgG against SARS-CoV-2. Our study showed that although Ct value might not have any relation with severity of the diseases but is associated with the antibody response among the SARS-CoV-2 infected individual.

scholarly journals Cell interactions in the suppression of in vitro antibody responses.

1976 ◽  
Vol 143 (6) ◽  
pp. 1421-1428 ◽  
Author(s):  
C E Calkins ◽  
S Orbach-Arbouys ◽  
O Stutman ◽  
R K Gershon
Keyword(s):  
B Lymphocytes ◽  
Immune Cells ◽  
Suppressor Cells ◽  
Antibody Responses ◽  
Secondary Antibody ◽  
Spleen Cells ◽  
Homeostatic Mechanism ◽  
Normal Spleen ◽  
Cells Cultured

Normal T and immune B lymphocytes interact in a fashion that leads to suppression of the immune response. Normal spleen cells added to cultures of primed spleen cells specifically suppressed both the IgM and IgG secondary antibody response of the primed cells to less than 30% of the response of the immune cells cultured alone. Cell crowding as a possible in vitro artifact was ruled out. The suppression was specific for the priming antigen, even when the specific and nonspecific antigens were included in the same cultures. Suppression required both normal T and immune B cells to be present in culture. We suggest that the immune population produces a signal that can induce normal T cells to become specific suppressor cells. This form of interaction may represent an important regulatory (homeostatic) mechanism in the immune system.

scholarly journals Density of surface immunoglobulin and capping on rat B lymphocytes. I. Changes with aging.

1979 ◽  
Vol 149 (2) ◽  
pp. 416-423 ◽  
Author(s):  
B A Woda ◽  
J D Feldman
Keyword(s):  
B Lymphocytes ◽  
Colchicine Treatment ◽  
Cell Populations ◽  
Low Density ◽  
Cell Sorter ◽  
Lewis Rats ◽  
Surface Immunoglobulin ◽  
Surface Immunoglobulins ◽  
The Difference ◽  
Kinetics Of

The rate of capping and shedding of cross-linked surface immunoglobulins (SIg) was slower in old Lewis rats (greater than 24 mo) than in young Lewis rats (3-4 mo). Analysis of spleen cell populations with the fluorescence-activated cell sorter indicated that with aging there was a loss of cells with a high density of SIg. Cells with the highest density of SIg capped and shed cross-linked SIg faster than cells with a low density of SIg. The alteration in density of SIg may account for the difference in capping kinetics. Colchicine treatment increased the rate of capping of lymphocytes from young animals, but had no effect on the capping kinetics of lymphocytes from old animals.

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