scholarly journals Cooperative effects of colony-stimulating factor 1 and recombinant interleukin 2 on proliferation and induction of cytotoxicity of macrophage precursors generated from mouse bone marrow cell cultures.

1989 ◽  
Vol 169 (3) ◽  
pp. 973-986 ◽  
Author(s):  
H Li ◽  
R Schwinzer ◽  
M Baccarini ◽  
M L Lohmann-Matthes
Keyword(s):  
Bone Marrow ◽  
Marrow Cell ◽  
Interleukin 2 ◽  
Culture Conditions ◽  
Mouse Bone Marrow ◽  
Nk Activity ◽  
Proliferating Cells ◽  
Mouse Bone Marrow Cell ◽  
Cooperative Effects

Precursor cells for NK activity, present in the light fraction of fresh mouse bone marrow, were cultivated in vitro in the presence of either CSF-1, IL-2, or a combination of both factors. In the presence of only CSF-1, strong proliferation was induced. Cells quickly passed the macrophage precursor stage and matured to typical macrophages. Neither granula formation nor NK activity were induced. Under culture conditions with only IL-2 NK activity had developed after 3 d, however, no significant proliferation occurred. In the presence of both factors strong proliferation was induced, and concomitantly, granula formation and NK activity developed. Apparently, proliferation depended on CSF-1 and granula formation, and NK cytotoxicity was induced by IL-2. When proliferating cells with strong anti-YAC-1 activity from a culture in CSF-1 plus IL-2 were further cultivated in only IL-2, the content of granula further increased, whereas proliferation gradually stopped. In contrast, when these cells from CSF-1 plus IL-2 culture were further cultivated in only CSF-1, granula disappeared and NK activity was lost, whereas sustained proliferation and differentiation to macrophages occurred. Only under culture conditions with both factors were proliferation and NK activity both maintained. More than 90% of cells from a 3-d culture in CSF-1 plus IL-2 expressed the NK 1.1. marker, whereas F4/80 was only marginally detected by FACS analysis. After two further days in culture, 70% of the cells expressed F4/80 and 60% coexpressed NK 1.1. and F4/80. By setting the size scatter in order to gate for large granular cells, a population was obtained with 100% coexpression of NK1.1. and F4/80. The data indicate that early cells of the macrophage lineage can develop into different functional and morphological directions depending on the varying influence of IL-2 and CSF-1.

scholarly journals Dry Mass Distribution Changes in Mouse Bone Marrow Cell Population during the First 24 Hours after X—Irradiation as Determined by Interfereometry

Blood ◽  
1965 ◽  
Vol 25 (3) ◽  
pp. 299-309 ◽  
Author(s):  
HUN LEE ◽  
VICTOR RICHARDS ◽  
MARIA MAICHLE
Keyword(s):  
Bone Marrow ◽  
Mass Distribution ◽  
Bone Marrow Cells ◽  
Marrow Cell ◽  
Dry Mass ◽  
Steady Increase ◽  
Mouse Bone Marrow ◽  
Proliferating Cells ◽  
Mouse Bone Marrow Cell ◽  
Total Body

Abstract LAF1 mice were treated with a total-body dose of 800 r. x-ray. The dry mass distribution of the femur bone marrow cells was determined at different intervals postirradiation. The nonproliferating cells showed no significant dry mass change, whereas the proliferating cells of the myelocyte series had a steady increase in mean dry mass per cell. Few cells with the maximum dry mass increase survived at the end of 48 hours postirradiation. The dry mass distribution formed characteristic patterns for each postirradiation interval studied as the proliferating cells shifted to higher dry mass values with accompanied increase in cell sizes.

Structural, luminescence and geno/cytoxicity study of carbon dots derived from Opuntia ficus-indica (L.) Mill

2020 ◽  
Vol 44 (3) ◽  
pp. 942-950
Author(s):  
Eduardo Madrigal-Bujaidar ◽  
Genaro Ivan Cerón-Montes ◽  
Joan Reyes-Miranda ◽  
Erasto Vergara-Hernández ◽  
Isela Álvarez-González ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Proliferation ◽  
Bone Marrow Cell ◽  
Carbon Dots ◽  
Marrow Cell ◽  
Mouse Bone Marrow ◽  
Opuntia Ficus Indica ◽  
Mouse Bone Marrow Cell

Carbon dots derived from nopal significantly increase the number of micronuclei in mouse erythrocytes and inhibit mouse bone marrow cell proliferation.

The use of metformin to enhance radiation effects on M1 macrophage subtype polarization in bone marrow-derived macrophage in glioblastoma tumor environment.

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. e13513-e13513
Author(s):  
Fei Wang ◽  
Nan Zhao ◽  
Chi Lin ◽  
Chi Zhang
Keyword(s):  
Bone Marrow ◽  
Tumor Microenvironment ◽  
Radiation Effects ◽  
Culture Conditions ◽  
Mouse Bone Marrow ◽  
M1 Macrophage ◽  
Tumor Environment ◽  
In Trans ◽  
High Concentrations

e13513 Background: Given the clinical relevance of tumor-associated macrophage (TAM) with its pro-tumor role and as a cell type compromising a large portion in glioblastoma (GBM), reversing the imbalance of TAM polarization in the tumor environment has emerged as a promising novel field for GBM treatment. Radiation therapy (RT) is the standard treatment for GBM patients after surgery, which has been shown to transiently induce M1 polarization of macrophage (M1Ø). Recent studies suggested that metformin could also promote M1Ø in tumor microenvironment. We thus postulate that metformin may enhance and sustain the M1-inducing effect of radiation in GBM. Methods: We first examined the polarization effect of metformin (0.1mM, 1mM and 2mM) on mouse bone marrow-derived macrophage (BMDM) cultured in GBM tumor environment, including media conditioned by GBM cells in monolayer culture or tumor spheres as well as in trans-well co-culturing system. We irradiated GBM cells with different doses (2 Gy, 8 Gy, and 20 Gy) after the treatment of Metformin at various time points; then we used conditioned media to treat BMDM either cultured alone or co-cultured with GBM cells in trans-well system for 24 or 48 hours. A separate set of experiment was conducted by first irradiating GBM cells and then co-culturing them with BMDM at 24 or 48 hours after radiation with metformin added at the start of co-culture. Percentage of various subtypes of BMDM was calculated after flow cytometry. Results: High concentrations of metformin (1mM and 2mM) significantly increased M1Ø and inhibited M2Ø in all culture conditions. Co-culture with irradiated GBM cells or treatment with medium conditioned by irradiated GBM cells could temporally induce M1Ø polarization in BMDM, with the effects being RT dose-dependent. Metformin at high concentrations further promoted M1Ø and suppressed M2Ø polarization in those conditions mimicking tumor microenvironment. This enhancing effect was sustained for at least 48 hours. Conclusions: Metformin at mili-molar concentrations significantly enhances the effects of radiation on M1Ø polarization in BMDM in vitro.

Effects of lead on osteoclast-like cell formation in mouse bone marrow cell cultures

1994 ◽  
Vol 54 (2) ◽  
pp. 165-169 ◽  
Author(s):  
T. Miyahara ◽  
H. Komiyama ◽  
A. Miyanishi ◽  
M. Matsumoto ◽  
W. Xue-Ya ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Cell Cultures ◽  
Marrow Cell ◽  
Cell Formation ◽  
Mouse Bone Marrow ◽  
Mouse Bone Marrow Cell
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