Dry Mass Distribution Changes in Mouse Bone Marrow Cell Population during the First 24 Hours after X—Irradiation as Determined by Interfereometry

Abstract LAF1 mice were treated with a total-body dose of 800 r. x-ray. The dry mass distribution of the femur bone marrow cells was determined at different intervals postirradiation. The nonproliferating cells showed no significant dry mass change, whereas the proliferating cells of the myelocyte series had a steady increase in mean dry mass per cell. Few cells with the maximum dry mass increase survived at the end of 48 hours postirradiation. The dry mass distribution formed characteristic patterns for each postirradiation interval studied as the proliferating cells shifted to higher dry mass values with accompanied increase in cell sizes.

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Cooperative effects of colony-stimulating factor 1 and recombinant interleukin 2 on proliferation and induction of cytotoxicity of macrophage precursors generated from mouse bone marrow cell cultures.

Journal of Experimental Medicine ◽

10.1084/jem.169.3.973 ◽

1989 ◽

Vol 169 (3) ◽

pp. 973-986 ◽

Cited By ~ 31

Author(s):

H Li ◽

R Schwinzer ◽

M Baccarini ◽

M L Lohmann-Matthes

Keyword(s):

Bone Marrow ◽

Marrow Cell ◽

Interleukin 2 ◽

Culture Conditions ◽

Mouse Bone Marrow ◽

Nk Activity ◽

Proliferating Cells ◽

Mouse Bone Marrow Cell ◽

Cooperative Effects

Precursor cells for NK activity, present in the light fraction of fresh mouse bone marrow, were cultivated in vitro in the presence of either CSF-1, IL-2, or a combination of both factors. In the presence of only CSF-1, strong proliferation was induced. Cells quickly passed the macrophage precursor stage and matured to typical macrophages. Neither granula formation nor NK activity were induced. Under culture conditions with only IL-2 NK activity had developed after 3 d, however, no significant proliferation occurred. In the presence of both factors strong proliferation was induced, and concomitantly, granula formation and NK activity developed. Apparently, proliferation depended on CSF-1 and granula formation, and NK cytotoxicity was induced by IL-2. When proliferating cells with strong anti-YAC-1 activity from a culture in CSF-1 plus IL-2 were further cultivated in only IL-2, the content of granula further increased, whereas proliferation gradually stopped. In contrast, when these cells from CSF-1 plus IL-2 culture were further cultivated in only CSF-1, granula disappeared and NK activity was lost, whereas sustained proliferation and differentiation to macrophages occurred. Only under culture conditions with both factors were proliferation and NK activity both maintained. More than 90% of cells from a 3-d culture in CSF-1 plus IL-2 expressed the NK 1.1. marker, whereas F4/80 was only marginally detected by FACS analysis. After two further days in culture, 70% of the cells expressed F4/80 and 60% coexpressed NK 1.1. and F4/80. By setting the size scatter in order to gate for large granular cells, a population was obtained with 100% coexpression of NK1.1. and F4/80. The data indicate that early cells of the macrophage lineage can develop into different functional and morphological directions depending on the varying influence of IL-2 and CSF-1.

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Identification of Fc epsilon RIneg mast cells in mouse bone marrow cell cultures. Use of a monoclonal anti-p161 antibody.

Journal of Experimental Medicine ◽

10.1084/jem.182.2.575 ◽

1995 ◽

Vol 182 (2) ◽

pp. 575-579 ◽

Cited By ~ 8

Author(s):

C A Kinzer ◽

A D Keegan ◽

W E Paul

Keyword(s):

Bone Marrow ◽

Mast Cell ◽

Mast Cells ◽

Bone Marrow Cells ◽

Marrow Cell ◽

Surface Glycoprotein ◽

Mouse Bone Marrow ◽

Short Term ◽

Mouse Bone Marrow Cell ◽

Cell Surface Glycoprotein

A monoclonal hamster antibody (K-1) specific for a 161-kD mast cell surface glycoprotein was derived. p161 is expressed on normal and cultured mast cells and on some macrophages, but not on basophils or other hematopoietic cells. A population of Fc epsilon Rneg cells expressing p161 was found in short term cultures of bone marrow cells grown in interleukin (IL)-3. These cells were purified and propagated for extended periods in IL-3. They express c-kit and Fc gamma RII/III, contain alcian blue-positive granules and histamine, and secrete IL-3 in response to ionomycin treatment. Their morphology is consistent with that of mast cells. We propose that they represent Fc epsilon RIneg mast cells that can be detected and purified because of their p161 expression.

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Interaction of Triiodothyronine With 1α,25-Dihydroxyvitamin D3 on Interleukin-6-Dependent Osteoclast-like Cell Formation in Mouse Bone Marrow Cell Cultures

Bone ◽

10.1016/s8756-3282(97)00291-3 ◽

1998 ◽

Vol 22 (4) ◽

pp. 341-346 ◽

Cited By ~ 24

Author(s):

C Schiller ◽

R Gruber ◽

G.-M Ho ◽

K Redlich ◽

H.-J Gober ◽

...

Keyword(s):

Bone Marrow ◽

Bone Marrow Cell ◽

Cell Cultures ◽

Interleukin 6 ◽

Marrow Cell ◽

Cell Formation ◽

Mouse Bone Marrow ◽

Dihydroxyvitamin D3 ◽

Mouse Bone Marrow Cell

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Effects of neuraminidase on the regulation of erythropoiesis

Blood ◽

10.1182/blood.v63.4.784.784 ◽

1984 ◽

Vol 63 (4) ◽

pp. 784-788 ◽

Cited By ~ 3

Author(s):

VF LaRussa ◽

F Sieber ◽

LL Sensenbrenner ◽

SJ Sharkis

Keyword(s):

Bone Marrow ◽

Colony Formation ◽

Bone Marrow Cells ◽

Marrow Cell ◽

Colony Growth ◽

Mouse Bone Marrow ◽

Continuous Exposure ◽

Low Concentrations ◽

High Concentrations ◽

Marrow Cells

Abstract In this article, we present evidence that sialic acid-containing surface components play a role in the regulation of erythropoiesis. A 1- hr exposure of mouse bone marrow cells to high concentrations of neuraminidase reduced erythroid colony formation. Coculture of 10(6) untreated thymocytes with neuraminidase-treated bone marrow cells restored erythroid colony growth. Neuraminidase-treated thymocytes retained their ability to suppress erythroid colony formation by untreated marrow cells, but lost their ability to enhance erythroid colony formation. Continuous exposure to low concentrations of neuraminidase enhanced erythroid bone marrow cell colony growth in response to a suboptimal dose of erythropoietin.

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Structural, luminescence and geno/cytoxicity study of carbon dots derived from Opuntia ficus-indica (L.) Mill

New Journal of Chemistry ◽

10.1039/c9nj03771c ◽

2020 ◽

Vol 44 (3) ◽

pp. 942-950

Author(s):

Eduardo Madrigal-Bujaidar ◽

Genaro Ivan Cerón-Montes ◽

Joan Reyes-Miranda ◽

Erasto Vergara-Hernández ◽

Isela Álvarez-González ◽

...

Keyword(s):

Bone Marrow ◽

Cell Proliferation ◽

Bone Marrow Cell ◽

Carbon Dots ◽

Marrow Cell ◽

Mouse Bone Marrow ◽

Opuntia Ficus Indica ◽

Mouse Bone Marrow Cell

Carbon dots derived from nopal significantly increase the number of micronuclei in mouse erythrocytes and inhibit mouse bone marrow cell proliferation.

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Calcium ionophore but not phorbol ester promotes eicosanoids release by proliferating interleukin-3-dependent bone marrow cells

Blood ◽

10.1182/blood.v76.8.1586.1586 ◽

1990 ◽

Vol 76 (8) ◽

pp. 1586-1592 ◽

Cited By ~ 2

Author(s):

Y Shibata ◽

PG McCaffrey ◽

H Sato ◽

Y Oghiso

Keyword(s):

Bone Marrow ◽

Cell Lines ◽

Phorbol Ester ◽

Bone Marrow Cells ◽

Marrow Cell ◽

Calcium Ionophore ◽

Mouse Bone Marrow ◽

Ionophore A23187 ◽

Interleukin 3 ◽

Marrow Cells

Abstract Eicosanoid release during multilineage hematopoiesis was assessed using freshly isolated mouse bone marrow cells cultured in the presence of interleukin-3 (IL-3) (10% WEHI-3 culture-conditioned medium). Cells that could release prostaglandin E2 (PGE2) when stimulated with calcium ionophore A23187, but not with phorbol ester (PMA), appeared within 4 days. The cells harvested on day 10 released 42 ng of PGE2/10(6) cells/mL after A23187 stimulation. Leukotriene B4 (LTB4) (4 ng/mL) was also detected after A23187 stimulation, but there was no detectable LTC4 (less than 0.5 ng/mL). Nonadherent bone marrow cells were isolated from 28-day cultures and cloned. All clones were strongly IL-3- dependent. Although other growth factors such as granulocyte colony- stimulating factor (G-CSF), granulocyte-macrophage CSF (GM-CSF), and CSF-1 failed to promote survival or support proliferation of the cells, three clones (11–1-A6, 3–2-D5, and 11–1-A1) showed significant increases in 3H-thymidine incorporation, respectively, after PMA treatment for 24 hours. Surviving cells displayed dominantly myeloid type morphology and phenotypic characteristics. The data suggest that IL-3 is important in the formation of PGE2-producing cells. In contrast to many macrophages (MO), neither the IL-3-dependent cell lines nor the IL-3-cultured bone marrow cells released significant amounts of PGE2 when stimulated with PMA or IL-3, although PMA and IL-3 both induced translocation of protein kinase C (PKC) to the membrane fraction. The lack of production of PGE2 and other eicosanoids by the PMA- and IL-3- stimulated cell lines was confirmed by measuring the release of 3H- arachidonic acid. The data suggest that in IL-3-dependent bone marrow cell lines the activation of eicosanoid metabolism requires elevated cellular Ca2+; PKC activation alone does not appear to be a sufficient stimulus.

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