scholarly journals Monoclonal antibody defining a molecule possibly identical to the p75 subunit of interleukin 2 receptor.

1989 ◽  
Vol 169 (4) ◽  
pp. 1323-1332 ◽  
Author(s):  
T Takeshita ◽  
Y Goto ◽  
K Tada ◽  
K Nagata ◽  
H Asao ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Interleukin 2 ◽  
High Affinity ◽  
Human T Cell ◽  
Low Concentrations ◽  
High Concentrations ◽  
Dependent Growth ◽  
T Cell Lines

A mouse hybridoma cell line, TU27, producing an mAb was established. TU27 mAb reacted with various human and Gibbon ape T cell lines bearing the IL-2R p75 (IL-2Rp75), but not with cell lines expressing only Tac antigen, IL-2Rp55, and numbers of its binding sites on cell surfaces were similar to those of high-affinity IL-2R. Radioimmunoprecipitation with TU27 mAb defined a molecule with a molecular mass of 75 kD on the surface of IL-2Rp75 bearing cells. TU27 mAb completely blocked IL-2 binding to IL-2Rp75 and to the high-affinity IL-2R but not to IL-2Rp55 composing the low-affinity IL-2R. The IL-2-dependent growth of a human T cell line, ILT-Mat, was significantly inhibited by TU27 mAb only at low concentrations of IL-2, and combination of TU27 mAb and H-31 mAb specific for IL-2Rp55 completely inhibited the cell growth even at high concentrations of IL-2. These data strongly suggest that TU27 mAb is specific for the human IL-2Rp75.

scholarly journals High-affinity receptor-mediated internalization and degradation of interleukin 2 in human T cells.

1986 ◽  
Vol 163 (3) ◽  
pp. 550-562 ◽  
Author(s):  
M Fujii ◽  
K Sugamura ◽  
K Sano ◽  
M Nakai ◽  
K Sugita ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Interleukin 2 ◽  
Culture Fluid ◽  
Type I ◽  
Temperature Dependent ◽  
High Affinity ◽  
Human T Cell ◽  
Human T Cells ◽  
High Affinity Receptor

Receptor-mediated internalization and degradation of IL-2 were investigated in cell lines carrying human T cell leukemia/lymphoma (lymphotrophic) virus type I (HTLV-I) and PHA-treated normal PBL. The HTLV-I-carrying cell lines ILT-Yan and TL-Mor, and the PBL expressed both high- and low-affinity IL-2-R. However, another HTLV-I-carrying T cell line, MT-1, expressed mainly low-affinity receptors. Greater than 50% of the IL-2 bound to high-affinity receptors was internalized within 10 min when these cells were incubated at 37 degrees C. The internalized IL-2 was rapidly degraded and the products were excreted into the culture fluid. The t1/2 of IL-2 degradation in these cells was estimated as 60-80 min at 37 degrees C. The internalization and degradation of IL-2 were both temperature dependent. Light-microscopic autoradiography with 3H-labeled IL-2 confirmed the internalization of IL-2, and suggested that some IL-2 might be carried to the nucleus.

scholarly journals Interleukin 2 induces antigen-reactive T cell lines to secrete BCGF-I.

1983 ◽  
Vol 158 (6) ◽  
pp. 2024-2039 ◽  
Author(s):  
M Howard ◽  
L Matis ◽  
T R Malek ◽  
E Shevach ◽  
W Kell ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
B Cells ◽  
B Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Interleukin 2 ◽  
Activated T Lymphocytes ◽  
T Cell Lines ◽  
Activated B Cells

Antigen-activated T lymphocytes produce within 24 h of stimulation a factor that is indistinguishable biochemically and functionally from the B cell co-stimulating growth factor, BCGF-I, originally identified in induced EL4 supernatants: Supernatants from antigen-stimulated T cell lines are not directly mitogenic for resting B cells, but synergize in an H-2-unrestricted manner with anti-Ig activated B cells to produce polyclonal proliferation but not antibody-forming-cell development; biochemical studies reveal the B cell co-stimulating factor present in antigen-stimulated T cell line supernatants is identical by phenyl Sepharose chromatography and isoelectric focusing (IEF) to EL4 supernatant BCGF-I. We thus conclude that normal T cells produce BCGF-I in response to antigenic stimulation. Analysis of the mechanism of BCGF-I production by antigen-stimulated T cells showed that optimum amounts of BCGF-I were obtained as quickly as 24 h post-stimulation, and that the factor producing cells in the T cell line investigated bore the Lyt-1+2- phenotype. As few as 10(4) T cells produced sufficient BCGF-I to support the proliferation of 5 X 10(4) purified anti-Ig activated B cells. Finally, the activation of normal T cell lines to produce BCGF-I required either antigen presented in the context of syngeneic antigen-presenting cells (APC) or interleukin 2 (IL-2).

scholarly journals Selective endothelial binding of interleukin-2-dependent human T-cell lines derived from different tissues.

1992 ◽  
Vol 89 (23) ◽  
pp. 11436-11440 ◽  
Author(s):  
M. Salmi ◽  
K. Granfors ◽  
M. Leirisalo-Repo ◽  
M. Hamalainen ◽  
R. MacDermott ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Interleukin 2 ◽  
Human T Cell ◽  
T Cell Lines ◽  
Different Tissues

scholarly journals Interleukin-2-dependent T-cell lines established from paroxysmal nocturnal hemoglobinuria patients

Blood ◽  
1994 ◽  
Vol 84 (1) ◽  
pp. 309-314
Author(s):  
H Nakakuma ◽  
S Nagakura ◽  
K Horikawa ◽  
M Hidaka ◽  
T Kawaguchi ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Surface ◽  
Cell Line ◽  
Cell Lines ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Control Cell ◽  
Interleukin 2 ◽  
Culture Cell ◽  
Adult T Cell Leukemia ◽  
T Cell Lines

Peripheral blood T lymphocytes obtained from two patients with paroxysmal nocturnal hemoglobinuria (PNH) were immortalized with human T-lymphotropic virus type 1 (HTLV-1). These cells showed interleukin-2 (IL-2)-dependent cell growth in culture. Cell surface analysis showed that they had the phenotype of a helper/inducer T subset that was positive for CD2, CD3, and CD4, but negative for CD8, similar to adult T-cell leukemia cells induced by HTLV-1. These cell lines lacked glycosylphosphatidylinositol (GPI)-anchored proteins, CDw52, CD55 (decay-accelerating factor; DAF), and CD59 on the cell surface, whereas intracellular DAF protein was detected. These T-subset cell lines with a PNH phenotype did not synthesize GPI anchor, whereas a control cell line, similarly prepared from the T cells of a healthy volunteer, produced the anchor. The control cells expressed CDw52, DAF, and CD59 on the cell surface and showed the phenotype of a helper/inducer subset. Southern blot analysis confirmed the clonality of each cell line. These CD4+ T-cell lines with a PNH phenotype and a subset-matched control counterpart could be a useful model for PNH investigation.

scholarly journals Interleukin 2 inhibits in vitro growth of human T cell lines carrying retrovirus.

1985 ◽  
Vol 161 (5) ◽  
pp. 1243-1248 ◽  
Author(s):  
K Sugamura ◽  
S Nakai ◽  
M Fujii ◽  
Y Hinuma
Keyword(s):  
T Cell ◽  
Growth Inhibition ◽  
Cell Lines ◽  
Specific Binding ◽  
Interleukin 2 ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
Human T Cell ◽  
T Cell Lines

Four human T cell lines, TL-Mor, TL-Su, TL-TerI, and TL-OmI, carrying human T cell leukemia virus (HTLV), were established previously. TL-Mor, TL-Su, and TL-TerI were derived from interleukin 2 (IL-2)-dependent parental cell lines cloned from peripheral blood leukocytes (PBL) of three healthy HTLV carriers, while TL-OmI was directly established from PBL of a patient with adult T cell leukemia. These four TL cell lines grow autonomously without IL-2. When they were cultured in the presence of IL-2, their growth was inhibited after a few days. This growth inhibition depended on the dose of IL-2, and the effective dose significantly promoted growth of their parental IL-2-dependent cell lines. The growth inhibition is demonstrated to be due to specific binding of IL-2 to receptors on the TL cells.

Characteristics of Interleukin 2 and Interleukin 4 Dependent T Cell Lines Infected with HTLV-1 in Vitro

1997 ◽  
Vol 10 (3) ◽  
pp. 189-194 ◽  
Author(s):  
B. Macchi ◽  
S. Grelli ◽  
C. Favalli ◽  
M. De Carli ◽  
Garaci ◽  
...  
Keyword(s):  
T Cell ◽  
Cord Blood ◽  
Cell Lines ◽  
Mononuclear Cells ◽  
Interleukin 2 ◽  
Normal Adult ◽  
Interleukin 4 ◽  
Human T Cell ◽  
T Cell Lines

Human T-cell leukemia/lymphoma virus type 1 (HTLV-1) is a lymphotropic retrovirus. Cells infected with HTLV-1 in vitro, when maintained in interleukin-2 (IL-2) can be immortalized, remaining for a long time strictly dependent on IL-2 addition. In this study we have compared the effect of interleukin-4 (IL-4) and interleukin-2 on HTLV-1 infection of cord blood or normal adult mononuclear cells. The results showed that either cultures of cord blood or normal adult T cells are susceptible to HTLV-1 infection in presence of IL-4 as well as IL-2. Moreover HTLV-1 infected cells in the presence of IL-4 survived only for a limited length of time in culture, while those grown in IL-2 showed the characteristics of immortalized cell lines. Moreover the profile of cytokine production showed a different pattern in HTLV-1 infected cell lines maintained in IL-4 or IL-2. This suggests that the lymphokines differently modulate retrovirus infection in vitro.

Overexpression of caveolin-1 in adult T-cell leukemia

Blood ◽  
2010 ◽  
Vol 115 (11) ◽  
pp. 2220-2230 ◽  
Author(s):  
Shigeki Sawada ◽  
Chie Ishikawa ◽  
Hiroe Tanji ◽  
Sawako Nakachi ◽  
Masachika Senba ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Signal Pathways ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
Caveolin 1 ◽  
Adult T Cell Leukemia ◽  
Human T Cell ◽  
T Cell Lines

AbstractCaveolin-1 is implicated in the regulation of signal pathways. Adult T-cell leukemia (ATL) is a T-cell malignancy causatively associated with human T-cell leukemia virus type 1 (HTLV-1). To determine the role of caveolin-1 in leukemogenesis, we examined caveolin-1 expression levels in HTLV-1–infected T-cell lines and ATL cells. These cells expressed high levels of caveolin-1 compared with uninfected T-cell lines and normal peripheral blood mononuclear cells (PBMCs). Caveolin-1–positive ATL cells were detected in ATL lymph nodes and skin lesions, and caveolin-1 was also detected in the plasma of patients with ATL. Infection of a human T-cell line, an epithelial cell line, and normal PBMCs with HTLV-1 induced caveolin-1 expression. The viral protein Tax transcriptionally activated caveolin-1 gene through nuclear factor-κB and cAMP response element binding protein signal pathways. HTLV-1–infected T-cell lines, and ATL cells are known to be resistant to transforming growth factor β (TGF-β)–induced growth inhibition. Caveolin-1 was colocalized with TGF-β type I receptor in HTLV-1–infected T-cell lines and suppressed TGF-β signaling. Caveolin-1 knockdown in an HTLV-1–infected T-cell line exhibited susceptibility to TGF-β. Thus, we describe a new function for Tax, repression of TGF-β signaling through caveolin-1 expression, which may play a critical role in ATL leukemogenesis.

scholarly journals Interleukin-2-dependent T-cell lines established from paroxysmal nocturnal hemoglobinuria patients

Blood ◽  
1994 ◽  
Vol 84 (1) ◽  
pp. 309-314 ◽  
Author(s):  
H Nakakuma ◽  
S Nagakura ◽  
K Horikawa ◽  
M Hidaka ◽  
T Kawaguchi ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Surface ◽  
Cell Line ◽  
Cell Lines ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Control Cell ◽  
Interleukin 2 ◽  
Culture Cell ◽  
Adult T Cell Leukemia ◽  
T Cell Lines

Abstract Peripheral blood T lymphocytes obtained from two patients with paroxysmal nocturnal hemoglobinuria (PNH) were immortalized with human T-lymphotropic virus type 1 (HTLV-1). These cells showed interleukin-2 (IL-2)-dependent cell growth in culture. Cell surface analysis showed that they had the phenotype of a helper/inducer T subset that was positive for CD2, CD3, and CD4, but negative for CD8, similar to adult T-cell leukemia cells induced by HTLV-1. These cell lines lacked glycosylphosphatidylinositol (GPI)-anchored proteins, CDw52, CD55 (decay-accelerating factor; DAF), and CD59 on the cell surface, whereas intracellular DAF protein was detected. These T-subset cell lines with a PNH phenotype did not synthesize GPI anchor, whereas a control cell line, similarly prepared from the T cells of a healthy volunteer, produced the anchor. The control cells expressed CDw52, DAF, and CD59 on the cell surface and showed the phenotype of a helper/inducer subset. Southern blot analysis confirmed the clonality of each cell line. These CD4+ T-cell lines with a PNH phenotype and a subset-matched control counterpart could be a useful model for PNH investigation.

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