scholarly journals Movies, measurement, and modeling

2005 ◽  
Vol 201 (4) ◽  
pp. 501-504 ◽  
Author(s):  
Colleen Witt ◽  
Subhadip Raychaudhuri ◽  
Arup K. Chakraborty
Keyword(s):  
Time Lapse ◽  
Three Dimensions ◽  
Mechanistic Models ◽  
Mechanistic Insight ◽  
Mechanical Methods ◽  
Statistical Mechanical ◽  
Computational Resources ◽  
Time Lapse Imaging ◽  
Insight Into

Immunological phenomena that were once deduced from genetic, biochemical, and in situ approaches are now being witnessed in living color, in three dimensions, and in real time. The information in time-lapse imaging can provide valuable mechanistic insight into a host of processes, from cell migration to signal transduction. What we need now are methods to quantitate these new visual data and to exploit computational resources and statistical mechanical methods to develop mechanistic models.

scholarly journals Time-lapse imaging of particle invasion and deposition in porous media using in situ X-ray radiography

2019 ◽  
Vol 177 ◽  
pp. 384-391 ◽  
Author(s):  
Jose R.A. Godinho ◽  
Kuhan Chellappah ◽  
Ian Collins ◽  
Pei Ng ◽  
Megan Smith ◽  
...  
Keyword(s):  
Porous Media ◽  
Time Lapse ◽  
X Ray ◽  
Time Lapse Imaging

scholarly journals Accumulation of actin in subsets of pioneer growth cone filopodia in response to neural and epithelial guidance cues in situ.

1993 ◽  
Vol 123 (4) ◽  
pp. 935-948 ◽  
Author(s):  
T P O'Connor ◽  
D Bentley
Keyword(s):  
Growth Cone ◽  
Central Region ◽  
Time Lapse ◽  
Growth Cones ◽  
Target Cells ◽  
Rhodamine Phalloidin ◽  
Intermediate Target ◽  
Guidance Cues ◽  
Time Lapse Imaging

Directed outgrowth of neural processes must involve transmission of signals from the tips of filopodia to the central region of the growth cone. Here, we report on the distribution and dynamics of one possible element in this process, actin, in live growth cones which are reorienting in response to in situ guidance cues. In grasshopper embryonic limbs, pioneer growth cones respond to at least three types of guidance cues: a limb axis cue, intermediate target cells, and a circumferential band of epithelial cells. With time-lapse imaging of intracellularly injected rhodamine-phalloidin and rhodamine-actin, we monitored the distribution of actin during growth cone responses to these cues. In distal limb regions, accumulation of actin in filopodia and growth cone branches accompanies continued growth, while reduction of actin accompanies withdrawal. Where growth cones are reorienting to intermediate target cells, or along the circumferential epithelial band, actin selectively accumulates in the proximal regions of those filopodia that have contacted target cells or are extending along the band. Actin accumulations can be retrogradely transported along filopodia, and can extend into the central region of the growth cone. These results suggest that regulation and translocation of actin may be a significant element in growth cone steering.

Mechanistic Insight into the Catalytic Oxidation of Cyclohexane over Carbon Nanotubes: Kinetic and In Situ Spectroscopic Evidence

2013 ◽  
Vol 19 (30) ◽  
pp. 9818-9824 ◽  
Author(s):  
Xixian Yang ◽  
Hongjuan Wang ◽  
Jing Li ◽  
Wenxu Zheng ◽  
Rong Xiang ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Catalytic Oxidation ◽  
Spectroscopic Evidence ◽  
Mechanistic Insight ◽  
Insight Into

scholarly journals Structural resolution and mechanistic insight into hydrogen adsorption in flexible ZIF-7

2021 ◽  
Author(s):  
Ryan A. Klein ◽  
Sarah Shulda ◽  
Philip A. Parilla ◽  
Pierre Le Magueres ◽  
Rachelle K. Richardson ◽  
...  
Keyword(s):  
Neutron Diffraction ◽  
Hydrogen Adsorption ◽  
Gas Storage ◽  
Powder Neutron Diffraction ◽  
Mechanistic Insight ◽  
Insight Into

Hydrogen induced flexibility in MOFs can be leveraged to increase useable gas storage capacities. Here hydrogen adsorption isothermal and in situ powder neutron diffraction measurements combine to reveal the mechanism driving flexibility in ZIF-7.

Growth cone dynamics of embryonic grasshopper pioneer neurons in situ

1995 ◽  
Vol 53 ◽  
pp. 802-803
Author(s):  
Tim P. O'Connor
Keyword(s):  
Nervous System ◽  
Growth Cone ◽  
Time Lapse ◽  
Pioneer Neurons ◽  
The Central Nervous System ◽  
Neuronal Growth Cone ◽  
Time Lapse Imaging ◽  
Guidance Information ◽  
Extracellular Environment

During development of the nervous system, neurons extend axons over relatively long distances to contact their targets. A variety of molecules in the extracellular environment are instrumental in guiding a neuronal process. The motile tip of the process, the growth cone, senses and transduces this guidance information, resulting in a local reorganization and consolidation of the cytoskeleton. Although much work has been dedicated to isolating the molecules that guide a neuronal growth cone, relatively little is known about the dynamic processes that occur when a growth cone turns in response to guidance information. Recently, a number of biological systems have been developed that enable time lapse imaging of growth cones as they extend axons in situ. One of these systems is the embryonic grasshopper limb fillet.In the grasshopper embryo, a pair of sibling neurons, named the Til pioneers, are the first neurons to extend axons toward the central nervous system (CNS).

Characterisation of SiCf/SiC Specimens Using an In-Situ Tensile Stage Within a Scanning Electron Microscope

2019 ◽  
Author(s):  
Steven P. Jordan ◽  
Martin R. Bache ◽  
Christopher D. Newton ◽  
Louise Gale
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Time Lapse ◽  
Image Correlation ◽  
Small Scale ◽  
Initiation And Propagation ◽  
Gauge Section ◽  
Time Lapse Imaging ◽  
Scanning Electron

Abstract The present paper will introduce the use of scanning electron microscope based, in-situ tensile testing as a method of detecting cracking in a SiCf/SiC CMC at room temperature. Small scale tensile specimens were prepared, but still sampling multiple longitudinal and transverse fibre tows. Monotonic loading was applied to initiate cracking, whilst contemporary time lapse imaging and retrospective digital image correlation recorded the development of these cracks at the specimen surface. Examples of strain localization, crack initiation and propagation will be presented for a plain gauge section specimen and single edge notched specimen. The critical combination of SEM imaging together with real time loading, in order to identify microscopic cracking in this CMC system, will be demonstrated.

scholarly journals A new mode of pancreatic islet innervation revealed by live imaging in zebrafish

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Yu Hsuan Carol Yang ◽  
Koichi Kawakami ◽  
Didier YR Stainier
Keyword(s):  
Neural Crest ◽  
Pancreatic Islets ◽  
Pancreatic Islet ◽  
Endocrine Cells ◽  
Close Contact ◽  
Time Lapse ◽  
Sensory Nerves ◽  
Time Lapse Imaging ◽  
Insight Into

Pancreatic islets are innervated by autonomic and sensory nerves that influence their function. Analyzing the innervation process should provide insight into the nerve-endocrine interactions and their roles in development and disease. Here, using in vivo time-lapse imaging and genetic analyses in zebrafish, we determined the events leading to islet innervation. Comparable neural density in the absence of vasculature indicates that it is dispensable for early pancreatic innervation. Neural crest cells are in close contact with endocrine cells early in development. We find these cells give rise to neurons that extend axons toward the islet as they surprisingly migrate away. Specific ablation of these neurons partly prevents other neurons from migrating away from the islet resulting in diminished innervation. Thus, our studies establish the zebrafish as a model to interrogate mechanisms of organ innervation, and reveal a novel mode of innervation whereby neurons establish connections with their targets before migrating away.

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