scholarly journals AN ETIOLOGIC CONSIDERATION OF DONOVANIA GRANULOMATIS CULTIVATED FROM GRANULOMA INGUINALE (THREE CASES) IN EMBRYONIC YOLK

1945 ◽  
Vol 81 (1) ◽  
pp. 25-40 ◽  
Author(s):  
Katherine Anderson ◽  
W. A. DeMonbreun ◽  
E. W. Goodpasture
Keyword(s):  
Epithelial Cells ◽  
Human Tissue ◽  
New Genus ◽  
Original Description ◽  
Chick Embryos ◽  
Cultural Characteristics ◽  
Pure Strain ◽  
Experimental Animals ◽  
Granuloma Inguinale

1. A microorganism identical with that originally described by Anderson has been cultivated in pure strain from two additional cases of granuloma inguinale by means of inoculating the yolk of chick embryos with uncontaminated human tissue containing Donovan bodies. 2. The morphological and cultural characteristics of the three isolated strains are described and discussed. 3. This microorganism has been cultivated in vitro only in media containing embryonic yolk. It failed to grow on any of a variety of artificial media. 4. It has not proved to be pathogenic for common experimental animals. 5. The Donovan body is reproduced in the epithelial cells of the yolk sac and in the yolk. The microorganism evidently reproduces both extracellularly and intracellularly. 6. The microorganism produces in culture antigens that elicit immune reactions in the skin and serum of granuloma inguinale patients. 7. This microorganism is judged to be a bacterium and the etiological agent of granuloma inguinale. 8. It is proposed that these strains of the bacterium be the type of a new genus, Donovania, in recognition of Donovan's original description of the pathognomonic bodies of granuloma inguinale; and that the specific name granulomatis be applied to designate its relationship to the characteristic lesion of the disease.

scholarly journals A PURE STRAIN OF CARTILAGE CELLS IN VITRO

1922 ◽  
Vol 36 (4) ◽  
pp. 379-384 ◽  
Author(s):  
Albert Fischer
Keyword(s):  
Close Contact ◽  
Initial Growth ◽  
Chick Embryos ◽  
Small Lymphocyte ◽  
Pure Strain ◽  
Thin Membranes ◽  
Cartilage Cells ◽  
Cultivation In Vitro ◽  
Large Nucleolus

1. A strain of cartilage cells, obtained from the pars cartilago scleræ of the eye of chick embryos, has been cultivated for more than 3 months in vitro. 2. The initial growth of the cartilage was possible only on the free surface of the coagulum. 3. The hyaline substance disappeared during cultivation in vitro. The succeeding stages of a transformation from small, lymphocyte-like cells into large, spindle-shaped cells were observed. The cartilage cells were spindle-shaped and grew in close contact, forming thin membranes. In surface-grown cartilage cells, the nucleus, usually containing one large nucleolus, stained less deeply than the cytoplasm. 4. The rate of growth of cartilage was slower than that of fibroblasts and epithelium. After cultivation on the surface of the coagulum, the cartilage cells could multiply even when embedded in the coagulum. But their growth was less extensive and uniform.

scholarly journals Intestinal cyto differentiation in vitro of chick stomach endoderm induced by the duodenal mesenchyme

Development ◽  
1984 ◽  
Vol 82 (1) ◽  
pp. 163-176
Author(s):  
Atsuko Ishizuya-Oka ◽  
Takeo Mizuno
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cells ◽  
Intestinal Epithelium ◽  
Intestinal Epithelial Cells ◽  
Goblet Cells ◽  
Secretory Cells ◽  
Chick Embryos ◽  
Intestinal Epithelial ◽  
Self Differentiation

The inductive action of duodenal mesenchyme on the cytodifferentiation of stomach endoderm in chick embryos was investigated in vitro with electron microscopy and immunofluorescence. Morphologically undifferentiated endoderm of the stomach of a 4-day embryo could differentiate only into a mucous secretory epithelium when cultured in the absence of mesenchyme. However, when cultivated in recombination with 6-day duodenal mesenchyme, most cells of 4-day stomach endoderm differentiated into intestinal absorptive cells possessing striated border and sucrase, and goblet cells, but not into stomach-type mucous secretory cells. In contrast, when 4-day stomach endoderm was cultured recombined with mesenchyme of embryonic digestive organs other than intestine, none of the stomach endoderm cells differentiated into intestinal epithelial cells. The competence of stomach endoderm for intestinal cytodifferentiation decreased rapidly with development, but remained until relatively later stages in the gizzard region. The present investigation demonstrates that duodenal mesenchyme can induce stomach endoderm, which has acquired the potency for self-differentiation into stomach-type epithelium, to cytodifferentiate into intestinal epithelium.

Electron probe x-ray microanalysis and electron microscopy of amino acid absorption and transport by the small intestine: inhibition by chemical poisons and correlation to the elemental composition of the epithelial cells

1986 ◽  
Vol 44 ◽  
pp. 134-135
Author(s):  
A. J. Tousimis
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Elemental Composition ◽  
Isotope Labeling ◽  
Structural Components ◽  
X Ray ◽  
Human Small Intestine ◽  
Transport Studies

The elemental composition of amino acids is similar to that of the major structural components of the epithelial cells of the small intestine and other tissues. Therefore, their subcellular localization and concentration measurements are not possible by x-ray microanalysis. Radioactive isotope labeling: I131-tyrosine, Se75-methionine and S35-methionine have been successfully employed in numerous absorption and transport studies. The latter two have been utilized both in vitro and vivo, with similar results in the hamster and human small intestine. Non-radioactive Selenomethionine, since its absorption/transport behavior is assumed to be the same as that of Se75- methionine and S75-methionine could serve as a compound tracer for this amino acid.

Human thymic epithelial cells maintain long-term survival of clonogenic myeloid and erythroid progenitor cells in vitro

2000 ◽  
Vol 111 (1) ◽  
pp. 363-370 ◽  
Author(s):  
Katsuto Takenaka ◽  
Mine Harada ◽  
Tomoaki Fujisaki ◽  
Koji Nagafuji ◽  
Shinichi Mizuno ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Progenitor Cells ◽  
Thymic Epithelial Cells ◽  
Erythroid Progenitor ◽  
Term Survival ◽  
Long Term Survival ◽  
Erythroid Progenitor Cells

Platelet Microtubule Subunit Proteins

1979 ◽  
Vol 42 (05) ◽  
pp. 1630-1633 ◽  
Author(s):  
A G Castle ◽  
N Crawford
Keyword(s):  
Epithelial Cells ◽  
Gel Electrophoresis ◽  
Blood Platelets ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Lens Epithelial Cells ◽  
Molecular Weights ◽  
Kinetics Of ◽  
Microtubular Structures

SummaryBlood platelets contain microtubule proteins (tubulin and HMWs) which can be polymerised “in vitro” to form structures which resemble the microtubules seen in the intact platelet. Platelet tubulin is composed of two non-identical subunits a and p tubulin which have molecular weights around 55,000 but can be resolved in alkaline SDS-polyacrylamide gel electrophoresis. These subunits associate as dimers with sedimentation coefficients of about 5.7 S although it is not known whether the dimer protein is a homo- or hetero-dimer. The dimer tubulin binds the anti-mitotic drug colchicine and the kinetics of this binding are similar to those reported for neurotubulins. Platelet microtubules also contain two HMW proteins which appear to be essential and integral components of the fully assembled microtubule. These proteins have molecular weights greater than 200,000 daltons. Fluorescent labelled antibodies to platelet and brain tubulins stain long filamentous microtubular structures in bovine lens epithelial cells and this pattern of staining is prevented by exposing the cells to conditions known to cause depolymerisation of cell microtubules.

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