Mutations of MLC1 (KIAA0027), Encoding a Putative Membrane Protein, Cause Megalencephalic Leukoencephalopathy with Subcortical Cysts

Treatment of red blood cells with calcium and ionomycin causes activation of the lipid scramblase, a putative membrane protein catalysing flip-flop of (phospho)lipids. Various fluorescent 1-oleoyl-2-[6(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino] caproyl (C6-NBD) analogues were tested for transbilayer movement across the plasma membrane of red blood cells. Among these phospholipid analogues were phosphatidylgalactose, phosphatidylmaltose and phosphatidylmaltotriose, which were obtained from C6-NBD-phosphatidylcholine by phospholipase D-catalysed transphosphatidylation. The inward movement after the onset of scrambling was monitored by extraction of the non-internalized probe with BSA. We demonstrate that both the amino group and the size of the headgroup determine the kinetics of lipid scrambling, and that lipids with a ceramide backbone migrate much more slowly than glycerophospholipids with the same headgroup.

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A putative membrane protein, Pho88p, involved in inorganic phosphate transport in

MGG Molecular & General Genetics ◽

10.1007/s004380050205 ◽

1996 ◽

Vol 251 (5) ◽

pp. 580 ◽

Cited By ~ 3

Author(s):

Chulee Yompakdee ◽

Nobuo Ogawa ◽

Satoshi Harashima ◽

Y. Oshima

Keyword(s):

Membrane Protein ◽

Inorganic Phosphate ◽

Phosphate Transport ◽

Putative Membrane Protein

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Cloning and characterization of CSP37, a novel gene encoding a putative membrane protein of Candida albicans.

Journal of Bacteriology ◽

10.1128/jb.179.15.4654-4663.1997 ◽

1997 ◽

Vol 179 (15) ◽

pp. 4654-4663 ◽

Cited By ~ 7

Author(s):

M Sentandreu ◽

A Nieto ◽

A Iborra ◽

M V Elorza ◽

J Ponton ◽

...

Keyword(s):

Candida Albicans ◽

Membrane Protein ◽

Gene Encoding ◽

Putative Membrane Protein ◽

Novel Gene

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A gene encoding a putative membrane protein homologous to the major facilitator superfamily of transporters maps upstream of the beta-glycosidase gene in the archaeon Sulfolobus solfataricus.

Journal of Bacteriology ◽

10.1128/jb.177.6.1614-1619.1995 ◽

1995 ◽

Vol 177 (6) ◽

pp. 1614-1619 ◽

Cited By ~ 16

Author(s):

A Prisco ◽

M Moracci ◽

M Rossi ◽

M Ciaramella

Keyword(s):

Membrane Protein ◽

Sulfolobus Solfataricus ◽

Major Facilitator Superfamily ◽

Gene Encoding ◽

Putative Membrane Protein ◽

Major Facilitator

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Kluyvera ascorbatacarrying themcr-1colistin resistance gene from hospital sewage

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01165-16 ◽

2016 ◽

pp. AAC.01165-16 ◽

Cited By ~ 10

Author(s):

Feifei Zhao ◽

Zhiyong Zong

Keyword(s):

Membrane Protein ◽

Resistance Gene ◽

Bacterial Species ◽

Colistin Resistance ◽

Gene Encoding ◽

Unknown Mechanism ◽

Putative Membrane Protein ◽

Kluyvera Ascorbata ◽

Hospital Sewage

The newly-identified plasmid-borne colistin resistance genemcr-1was found in aKluyvera ascorbatafrom hospital sewage in China.mcr-1was carried by a 57-kb self-transmissible IncI2 plasmid. Unlike the previous report,mcr-1was not associated with ISApl1and was inserted into a gene encoding a putative membrane protein due to an unknown mechanism. This study highlights thatmcr-1has spread to multiple bacterial species.

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