scholarly journals Kluyvera ascorbatacarrying themcr-1colistin resistance gene from hospital sewage

2016 ◽  
pp. AAC.01165-16 ◽  
Author(s):  
Feifei Zhao ◽  
Zhiyong Zong
Keyword(s):  
Membrane Protein ◽  
Resistance Gene ◽  
Bacterial Species ◽  
Colistin Resistance ◽  
Gene Encoding ◽  
Unknown Mechanism ◽  
Putative Membrane Protein ◽  
Kluyvera Ascorbata ◽  
Hospital Sewage

The newly-identified plasmid-borne colistin resistance genemcr-1was found in aKluyvera ascorbatafrom hospital sewage in China.mcr-1was carried by a 57-kb self-transmissible IncI2 plasmid. Unlike the previous report,mcr-1was not associated with ISApl1and was inserted into a gene encoding a putative membrane protein due to an unknown mechanism. This study highlights thatmcr-1has spread to multiple bacterial species.

scholarly journals Cloning and characterization of CSP37, a novel gene encoding a putative membrane protein of Candida albicans.

1997 ◽  
Vol 179 (15) ◽  
pp. 4654-4663 ◽  
Author(s):  
M Sentandreu ◽  
A Nieto ◽  
A Iborra ◽  
M V Elorza ◽  
J Ponton ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Membrane Protein ◽  
Gene Encoding ◽  
Putative Membrane Protein ◽  
Novel Gene

scholarly journals IncP Plasmid Carrying Colistin Resistance Gene mcr-1 in Klebsiella pneumoniae from Hospital Sewage

2016 ◽  
Vol 61 (2) ◽  
Author(s):  
Feifei Zhao ◽  
Yu Feng ◽  
Xiaoju Lü ◽  
Alan McNally ◽  
Zhiyong Zong
Keyword(s):  
Klebsiella Pneumoniae ◽  
Host Range ◽  
Resistance Gene ◽  
Insertion Sequence ◽  
Sequence Type ◽  
Broad Host Range ◽  
Colistin Resistance ◽  
Content Type ◽  
Broad Host Range Plasmid ◽  
Hospital Sewage

ABSTRACT A Klebsiella pneumoniae strain of sequence type 313 (ST313) recovered from hospital sewage was found carrying the plasmid-borne colistin resistance gene mcr-1, which was bracketed by two copies of the insertion sequence ISApl1 on a 57-kb self-transmissible IncP-type plasmid of a new IncP-1 clade. The carriage of mcr-1 on a self-transmissible broad-host-range plasmid highlights that mcr-1 has the potential to spread beyond the Enterobacteriaceae family.

scholarly journals Nearly Identical Plasmids Encoding VIM-1 and Mercury Resistance in Enterobacteriaceae from North-Eastern Germany

2021 ◽  
Vol 9 (7) ◽  
pp. 1345
Author(s):  
Stefan E. Heiden ◽  
Katharina Sydow ◽  
Stephan Schaefer ◽  
Ingo Klempien ◽  
Veronika Balau ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Bacterial Species ◽  
Genomic Analysis ◽  
Public Health Problem ◽  
Multidrug Resistant ◽  
Mercury Resistance ◽  
Major Public Health Problem ◽  
Eastern Germany ◽  
North Eastern ◽  
Resistance Plasmids

The emergence of carbapenemase-producing Enterobacteriaceae limits therapeutic options and presents a major public health problem. Resistances to carbapenems are mostly conveyed by metallo-beta-lactamases (MBL) including VIM, which are often encoded on resistance plasmids. We characterized four VIM-positive isolates that were obtained as part of a routine diagnostic screening from two laboratories in north-eastern Germany between June and August 2020. Whole-genome sequencing was performed to address (a) phylogenetic properties, (b) plasmid content, and (c) resistance gene carriage. In addition, we performed phenotypic antibiotic and mercury resistance analyses. The genomic analysis revealed three different bacterial species including C. freundii, E. coli and K. oxytoca with four different sequence types. All isolates were geno- and phenotypically multidrug-resistant (MDR) and the phenotypic profile was explained by the underlying resistance gene content. Three isolates of four carried nearly identical VIM-1-resistance plasmids, which in addition encoded a mercury resistance operon and showed some similarity to two publicly available plasmid sequences from sources other than the two laboratories above. Our results highlight the circulation of a nearly identical IncN-type VIM-1-resistance plasmid in different Enterobacteriaceae in north-eastern Germany.

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