The Use of Heteroduplex Analysis of Polymerase Chain Reaction Products to Support the Possible Transmission ofLegionella pneumophilaFrom a Malfunctioning Automobile Air Conditioner

2002 ◽  
Vol 23 (3) ◽  
pp. 145-147 ◽  
Author(s):  
Ahmet Pinar ◽  
Julio A. Ramirez ◽  
Laura L. Schindler ◽  
Richard D. Miller ◽  
James T. Summersgill
Keyword(s):  
Polymerase Chain Reaction ◽  
Legionella Pneumophila ◽  
Heteroduplex Analysis ◽  
Air Conditioner ◽  
Reaction Products ◽  
Chain Reaction ◽  
Long Distance ◽  
Automobile Air Conditioner ◽  
Legionnaires Disease ◽  
Polymerase Chain

AbstractAir conditioner condensates have not been previously associated with cases of Legionnaires' disease. We report the possible transmission of Legionella pneumophila serogroup 1 from a malfunctioning automobile air conditioning system's leaking water onto the floorboard of a car driven for a long distance by the patient. Heteroduplex analysis of polymerase chain reaction products was used to help establish an epidemiologic link between the water specimen and the patient.

Subtyping ofLegionella pneumophilaisolates by arbitrarily primed polymerase chain reaction

1995 ◽  
Vol 41 (9) ◽  
pp. 846-848 ◽  
Author(s):  
E. Ledesma ◽  
J. Llorca ◽  
M. A. Dasí ◽  
M. L. Camaró ◽  
E. Carbonell ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Legionella Pneumophila ◽  
Water Sources ◽  
Chain Reaction ◽  
Single Room ◽  
Legionnaires Disease ◽  
Polymerase Chain ◽  
Resort Hotel ◽  
Different Water Sources

Arbitrarily primed polymerase chain reaction (AP-PCR) was used to differentiate strains of Legionella pneumophila isolated from different water sources in a resort hotel in Benidorm, Alicante, Spain, where an outbreak of Legionnaires' disease occurred among a group of tourists between 65 and 80 years of age. All isolates were L. pneumophila serogroup 1, subtype Pontiac (Knoxville 1). Five different patterns (P1 to P5) were obtained by AP-PCR. The number of bands per pattern varied between 4 and 11. Patterns P1 and P2 represented 60 and 20% of L. pneumophila isolates, respectively. Since different subpopulations of L. pneumophila coexisted (up to three different AP-PCR patterns were identified in a single room), it was not possible to link an individual L. pneumophila strain to the occurrence of this outbreak.Key words: Legionella pneumophila, AP-PCR, subtyping, outbreak.

One-Step Directional Cloning of Blunt-Ended Polymerase Chain Reaction Products

2000 ◽  
Vol 287 (2) ◽  
pp. 349-352 ◽  
Author(s):  
Vincent W.S. Liu ◽  
Phillip Nagley ◽  
Hextan Y.S. Ngan
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction Products ◽  
Chain Reaction ◽  
Directional Cloning ◽  
One Step ◽  
Polymerase Chain
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