On-chip aqueous two-phase system (ATPS) formation, consequential self-mixing, and their influence on drop-to-drop aqueous two-phase extraction kinetics

An aqueous two-phase system of polyethylene glycol (PEG)/(NH4)2SO4 with the behavior of forming aqueous two-phase and the distribution rules of potassium glycyrrhizinate in the aqueous two-phase system were studied. Effects of the molecular weight of PEG, the mass fraction of PEG and (NH4)2SO4, and pH value were investigated. The result show that when the mass fraction of PEG1000 was 50%, the mass fraction of (NH4)2SO4 was 5.7%, and pH value was 6.5, the highest extraction recovery of potassium glycyrrhizinate could reach 99.7%. This aqueous two-phase extraction may provide a new technique for separation of potassium glycyrrhizinate.

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Determination of steroid hormones in milk using aqueous two-phase extraction coupled to liquid chromatography

Analytical Methods ◽

10.1039/c4ay03036b ◽

2015 ◽

Vol 7 (6) ◽

pp. 2514-2522 ◽

Cited By ~ 5

Author(s):

Na Li ◽

Xiao Yang ◽

Li Nian ◽

Zhibing Wang ◽

Lei Lei ◽

...

Keyword(s):

Liquid Chromatography ◽

Steroid Hormones ◽

Phase System ◽

Phase Extraction ◽

Two Phase ◽

Aqueous Two Phase System ◽

Aqueous Two Phase Extraction

An acetonitrile (ACN)-salt based aqueous two-phase system was developed and applied to the extraction of steroid hormones in milk.

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An automated centrifugal microfluidic assay for whole blood fractionation and isolation of multiple cell populations using an aqueous two-phase system

Lab on a Chip ◽

10.1039/d1lc00680k ◽

2021 ◽

Author(s):

Byeong-Ui Moon ◽

Liviu Clime ◽

Daniel Brassard ◽

Alex Boutin ◽

Jamal Daoud ◽

...

Keyword(s):

Human Blood ◽

Whole Blood ◽

Cell Populations ◽

Phase System ◽

Two Phase ◽

Microfluidic Platform ◽

Aqueous Two Phase System ◽

Separation Layers ◽

Multiple Cell ◽

On Chip

This paper describes an advanced on-chip whole human blood fractionation and cell isolation process combining an aqueous two-phase system to create complex separation layers with a centrifugal microfluidic platform to control and automate the assay.

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Study of Aqueous Two Phase System on Water Soluble Bio Oil

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.236-238.49 ◽

2011 ◽

Vol 236-238 ◽

pp. 49-53

Author(s):

Shao Peng Liu ◽

Zhi Min Zong ◽

Shan Shan Zhang ◽

Yue Xu ◽

Gui Zhen Gong ◽

...

Keyword(s):

Lower Layer ◽

Water Soluble ◽

Extraction Rate ◽

Phase System ◽

Two Phase ◽

Element Analysis ◽

Aqueous Two Phase System ◽

Bio Oil ◽

Infrared Spectrometer ◽

Aqueous Two Phase Extraction

This paper was aimed to study the aqueous two phase extraction (ATPE) on water soluble bio oil(SBO), in which ethanol, K2HPO4, NaH2PO4and (NH4)2SO4were selected to form the water soluble bio oil(SBO) aqueous two phase system (ATPS). After phase separation, the extraction rates and the volume ratios of the upper to lower layer of the ATPSs were investigeted. Both the upper and lower layers of these SBO ATPSs were analyzed by elemental analyzer and Fourier transformation infrared spectrometer (FTIR). The different extraction rates indicates the influnces of different salt during the phase saperation, of which NaH2PO4has less effect to SBO than the other two; K2HPO4may neutralize some organic acid; the lowest extraction rate of ethanol/(NH4)2SO4system indicates the effects of (NH4)2SO4to SBO. Too much ethanol will fech in more water, hence the optimizational ratio of the SBO ATPS should be SBO:salt:ethanol=10:10:5. In element analysis, the diclining of oxygen in all the upper layers and the increasing of nitrogion in both layers of (NH4)2SO4system indicates the abbility of the less oxygen components concentration of the ATPS and the reaction between (NH4)2SO4and SBO. According to FTIR test, the lack of absorption at 1515cm-1of all the lower layers indicates the completely transforming of phenols into the upper layers. Because of the high extraction rate and less influence to SBO, ethanol/NaH2PO4system may be the most suitable ATPS for SBO extraction.

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Probing the Mechanism of Aqueous Two-Phase System Formation for α-Elastin On-Chip

Journal of the American Chemical Society ◽

10.1021/ja037869c ◽

2003 ◽

Vol 125 (50) ◽

pp. 15630-15635 ◽

Cited By ~ 13

Author(s):

Yanjie Zhang ◽

Hanbin Mao ◽

Paul S. Cremer

Keyword(s):

Phase System ◽

Two Phase ◽

Aqueous Two Phase System ◽

System Formation ◽

On Chip

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Study on Separation and Purification of Resveratrol in Wine Grape Residue with Aqueous Two Phase Extraction Method

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.550-553.1743 ◽

2012 ◽

Vol 550-553 ◽

pp. 1743-1746 ◽

Cited By ~ 1

Author(s):

Ying Zi He ◽

Biao Wang ◽

Yan Zhuang ◽

Yu Ping Lu

Keyword(s):

Extraction Method ◽

Hplc Analysis ◽

Phase System ◽

Phase Extraction ◽

Wine Grape ◽

Separation And Purification ◽

Two Phase ◽

Organic Solvent Extraction ◽

Primary Separation ◽

Aqueous Two Phase Extraction

The separation and purification of resveratrol in wine grape residue with aqueous two phase extraction method were studied in this paper. Used identified ethanol - ammonium sulfate - water as the aqueous two-phase system, the wine grape residue extract substances separated well. By TLC and HPLC analysis, resveratrol content (13.3μg/ml) in aqueous two-phase extraction was higher than the results (2.342μg/ml) in organic solvent extraction. The results showed that the aqueous two phase extraction method could be used in primary separation and purification of resveratrol in wine grape residue.

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Study of caffeine and coumarin extraction kinetics in an aqueous two-phase system based on polyethylene glycol 1500

IOP Conference Series Materials Science and Engineering ◽

10.1088/1757-899x/1212/1/012020 ◽

2022 ◽

Vol 1212 (1) ◽

pp. 012020

Author(s):

I V Zinov’eva

Keyword(s):

Biological Activity ◽

Liquid Extraction ◽

Phase System ◽

Plant Origin ◽

Extraction Kinetics ◽

Two Phase ◽

Aqueous Two Phase System ◽

Food Industries ◽

Liquid Liquid Extraction ◽

Polyethylene Glycol 1500

Abstract Caffeine and coumarin are organic compounds of plant origin, which have biological activity and have found wide application in medicine, pharmaceutical, perfumery and food industries. Recovery of caffeine and coumarin from aqueous solutions using liquid-liquid extraction is the most effective method. In the present work the kinetic dependences of caffeine and coumarin in the aqueous two-phase system (ATPS) of PEG 1500 - Na2SO4 - H2O were experimentally obtained. It was established that equilibrium in the system is achieved after 17 minutes for caffeine and after 12 minutes for coumarin. The effect of ultrasound on the extraction rate of caffeine and coumarin in an aqueous two-phase system was also studied.

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