The binding of bovine serum albumin with dye molecules at different pH values. Fluorescence lifetime studies

Laser Physics ◽  
2021 ◽  
Vol 31 (6) ◽  
pp. 065601
Author(s):  
E S Gorodnichev ◽  
A A Kuleshova ◽  
O I Volkova ◽  
A M Saletsky
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fluorescence Lifetime ◽  
Bovine Serum ◽  
Dye Molecules ◽  
Ph Values ◽  
Lifetime Studies

Effect of the Association of Eosin Molecules on Their Interaction with Bovine Serum Albumin at Various pH Values

2019 ◽  
Vol 86 (5) ◽  
pp. 855-860
Author(s):  
E. S. Gorodnichev ◽  
A. A. Kuleshova ◽  
A. V. Bykov ◽  
A. M. Saletsky
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Ph Values

Bioceramic Hydroxyapatite Granules for Purification of Biotechnological Products

2011 ◽  
Vol 284-286 ◽  
pp. 1764-1769 ◽  
Author(s):  
Vitalijs Lakevics ◽  
Janis Locs ◽  
Dagnija Loca ◽  
Valentina Stepanova ◽  
Liga Berzina-Cimdina ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Phosphate Buffer ◽  
Bovine Serum ◽  
Phosphate Buffer Solution ◽  
Sorption Process ◽  
Buffer Solution ◽  
Ph Values ◽  
Bovine Serum Albumin Adsorption ◽  
Albumin Adsorption

Sorption experiments of bovine serum albumin (BSA) on hydroxyapatite (HAp) ceramic granules, prepared at three temperatures 900°C, 1000°C and 1150°C were performed at room temperature 18,6 °C and phosphate buffer, pH 5,83; 6.38 and 7,39. Thermal treatment contributed to the decrease of bovine serum albumin immobilization indicating that sorption process depended on HAp ceramics specific surface area and pH values of phosphate buffer solution. However, it was confirmed that granule size was also an important parameter for bovine serum albumin adsorption. As a result of these experiments, the most appropriate adsorption conditions and phosphate buffer pH values influence on to BSA sorption were analyzed.

The Rebinding Properties of Bovine Serum Albumin Imprinted Calcium Phosphate/Polyacrylate/Alginate Hybrid Polymer Microspheres

2010 ◽  
Vol 152-153 ◽  
pp. 1636-1640 ◽  
Author(s):  
Kong Yin Zhao ◽  
Jun Fu Wei ◽  
Jin Yang Zhou ◽  
Yi Ping Zhao ◽  
Guo Xiang Cheng
Keyword(s):  
Calcium Phosphate ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Polymer Microspheres ◽  
Hybrid Polymer ◽  
Ph Values ◽  
Factors Influencing ◽  
Recognition Property ◽  
Template Protein

Calcium phosphate/polyacrylate/alginate hybrid polymer microspheres with bovine serum albumin (BSA) embedded and coated on the surface were prepared with (NH4)2HPO4, sodium polyacrylate (SPA) and sodium alginate (SA) via Ca2+ crosslinking in inverse suspension. Rebinding behaviors of the microspheres were evaluated. The factors influencing the imprinting efficiency (IE) of imprinted microspheres were also studied, including the concentration of CaCl2, template content and pH values in rebinding solutions. Selectivity tests showed that the imprinted microspheres exhibited good recognition property for the template protein.

Effect of pH on the Formation of a Bovine Serum Albumin Layer on a Poly(stryren-co-maleic Acid) Surface

2010 ◽  
Vol 93-94 ◽  
pp. 583-586 ◽  
Author(s):  
Tippavan Hongkachern ◽  
Verawat Champreda ◽  
Toemsak Srikhirin ◽  
Thidarat Wangkam ◽  
Tanakorn Osotchan
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Maleic Acid ◽  
Bovine Serum ◽  
Quartz Crystal ◽  
Ph Value ◽  
Layer Formation ◽  
Force Microscopy ◽  
Ph Values ◽  
Atomic Force

The layer formation of bovine serum albumin (BSA) on a poly(styrene-co-maleic acid) (PSMA) surface was investigated by using quartz crystal microbalance (QCM) technique at various pH values. The formation of a BSA surface was examined by atomic force microscopy (AFM). To study the effect on the layer formation, the pH of solution was varied from 2 to 7.4 while the concentration of BSA was in the range of 0.01 to 5 mg/ml during the layer absorption. It was found that the BSA adsorption strongly depends on the pH of solution, and the concentration of BSA. The absorption layer occurred maximum at the pH value of 3.5 which resulted from the charge of PSMA and BSA molecules. The layer formation reached the saturate value at the concentration higher than 3 mg/ml. The molecular packing of the BSA layer at different pH values was determined by AFM and total mass change of QCM.

scholarly journals Diazaoxatriangulenium: synthesis of reactive derivatives and conjugation to bovine serum albumin

2016 ◽  
Vol 14 (3) ◽  
pp. 1091-1101 ◽  
Author(s):  
Ilkay Bora ◽  
Sidsel A. Bogh ◽  
Martin Rosenberg ◽  
Marco Santella ◽  
Thomas Just Sørensen ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fluorescence Lifetime ◽  
Bovine Serum

The red emitting, long fluorescence lifetime diazaoxatriangulenium dye DAOTA has been prepared with reactive NHS-ester and maleimide linkers for bioconjugation.

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