Quantification of 2D elemental distribution maps of intermediate-thick biological sections by low energy synchrotron μ-X-ray fluorescence spectrometry

2018 ◽  
Vol 13 (05) ◽  
pp. C05014-C05014 ◽  
Author(s):  
P. Kump ◽  
K. Vogel-Mikuš
2021 ◽  
Vol 10 (2) ◽  
pp. 51 ◽  
Author(s):  
Roberto Visalli ◽  
Gaetano Ortolano ◽  
Gaston Godard ◽  
Rosolino Cirrincione

Micro-Fabric Analyzer (MFA) is a new GIS-based tool for the quantitative extrapolation of rock microstructural features that takes advantage both of the characteristics of the X-ray images and the optical image features. Most of the previously developed edge mineral grain detectors are uniquely based on the physical properties of the X-ray-, electron-, or optical-derived images; not permitting the exploitation of the specific physical properties of each image type at the same time. More advanced techniques, such as 3D microtomography, permit the reconstruction of tridimensional models of mineral fabric arrays, even though adjacent mineral grain boundaries with the same atomic density are often not detectable. Only electron backscatter diffraction (EBSD) allows providing high-performing grain boundary detection that is crystallographically differentiated per mineral phase, even though it is relatively expensive and can be executed only in duly equipped microanalytical laboratories by suitably trained users. Instead, the MFA toolbox allows quantifying fabric parameters subdivided per mineral type starting from a crossed-polarizers high-resolution RGB image, which is useful for identifying the edges of the individual grains characterizing rock fabrics. Then, this image is integrated with a set of micro-X-ray maps, which are useful for the quantitative extrapolation of elemental distribution maps. In addition, all this is achieved by means of low-cost and easy-to-use equipment. We applied the tool on amphibolite, mylonitic-paragneiss, and -tonalite samples to extrapolate the particle fabric on different metamorphic rock types, as well as on the same sandstone sample used for another edge detector, which is useful for comparing the obtained results.


1998 ◽  
Vol 25 (4) ◽  
pp. 427 ◽  
Author(s):  
Simon A. Crawford ◽  
Alan T. Marshall ◽  
Sabine Wilkens

To determine if an increased aluminium (Al) sensitivity is the result of greater accumulation of Al in root apices, the quantity and distribution of Al in root apex cells of Danthonia linkii Kunth and Microlaena stipoides (Labill.) R.Br. was investigated by X-ray microanalysis. Seedlings were grown in nutrient solution with 0, 185 and 370 µM total Al for 24 h after which the terminal 5 mm of the roots were excised, rapidly frozen and embedded using freeze-substitution. Elemental distribution maps of root apices showed that Al had accumulated in the nuclei of root cap and meristematic cells in Al-stressed roots of D. linkii but not in M. stipoides. Al appeared to be co-localised with phosphorous (P) in the nuclei of these cells. Quantitation of Al revealed that Al-stressed root apex cells of D. linkiiaccumulated significantly more Al than M. stipoides. Exposure of D. linkii roots to Al resulted in substantial increases in the P content of root apex cells, and a significant positive correlation was found between Al and P in both root cap and meristematic cells. Analysis of intracellular structures showed that the majority of Al had accumulated in the nuclei of cells. A significant positive correlation was found between Al and P in the nuclei, but not in the cytoplasm or cell walls. No positive correlation was found between Al and P in root apex cells of M. stipoides.


2018 ◽  
Vol 13 (05) ◽  
pp. C05011-C05011
Author(s):  
A. Mantuano ◽  
C.L. Mota ◽  
A. Pickler ◽  
G. Sena ◽  
D. Braz ◽  
...  

Heritage ◽  
2021 ◽  
Vol 4 (3) ◽  
pp. 2224-2236
Author(s):  
Ana Martins ◽  
Ellen Davis ◽  
Talia Kwartler

Examining the painting Woman, Old Man, and Flower (1923–24) by Max Ernst with macro-X-ray fluorescence scanning (MA-XRF), X-ray radiography (XRR) as well as photography under ultraviolet (UVF), infrared reflected (IRR) and transmitted (IRT) illumination revealed the existence and sequence of three distinct paintings concealed under the final composition. The study confirmed a known and previously documented intermediate composition and uncovered two additional states: a very first state exposed by XRR, and a third state revealed in the elemental distribution maps obtained by MA-XRF. The complimentary images document the insertion, mutation, and concealing of several human and anthropomorphic subjects across the four layers, expanding our understanding of the painting and of Ernst’s collage-like pictorial development. In addition, a list of pigments is proposed based on the elemental information provided by MA-XRF, contributing to the technical literature devoted to the materials of Ernst’s paintings during the transitional period between Dada and Surrealism.


1988 ◽  
Vol 125 ◽  
Author(s):  
Helen I. Alten

ABSTRACTSamples of wet 13th-14th c. Grisaille window glass, visually sorted according to their extent of corrosion, were examined for physical changes while air-drying and solvent-drying. Polished cross-sections of the samples were examined with a JEOL-35CF SEM with a LINK AN 10,000 dispersive x-ray analyzer. Elemental distribution maps showed distinct alterations in the positioning of elements according to the corroded nature of the glass.


1988 ◽  
Vol 123 ◽  
Author(s):  
Helen I. Alten

AbstractSamples of wet 13th-14th c. Grisaille window glass, visually sorted according to their extent of corrosion, were examined for physical changes while air-drying and solvent-drying. Polished cross-sections of the samples were examined with a JEOL-35CF SEM with a LINK AN 10,000 dispersive x-ray analyzer. Elemental distribution maps showed distinct alterations in the positioning of elements according to the corroded nature of the glass.


Author(s):  
R. L. Stears

Because of the nature of the bacterial endospore, little work has been done on analyzing their elemental distribution and composition in the intact, living, hydrated state. The majority of the qualitative analysis entailed intensive disruption and processing of the endospores, which effects their cellular integrity and composition.Absorption edge imaging permits elemental analysis of hydrated, unstained specimens at high resolution. By taking advantage of differential absorption of x-ray photons in regions of varying elemental composition, and using a high brightness, tuneable synchrotron source to obtain monochromatic x-rays, contact x-ray micrographs can be made of unfixed, intact endospores that reveal sites of elemental localization. This study presents new data demonstrating the application of x-ray absorption edge imaging to produce elemental information about nitrogen (N) and calcium (Ca) localization using Bacillus thuringiensis as the test specimen.


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