scholarly journals Groundwater Characterization of Cihaur Watershed Basin, Batujajar and Adjacent, West Bandung District, West Java, Indonesia

2016 ◽  
Vol 29 ◽  
pp. 012027
Author(s):  
Fikri Noor Azy ◽  
Mohamad Sapari Dwi Hadian
Keyword(s):  
West Java ◽  
Groundwater Characterization

scholarly journals Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Widya Pintaka Bayu Putra ◽  
Saiful Anwar ◽  
Syahruddin Said ◽  
Romanos Albert Adhitya Indarto ◽  
Putri Wulandari
Keyword(s):  
Genetic Characterization ◽  
Rare Allele ◽  
Basic Information ◽  
Dominant Allele ◽  
West Java ◽  
Pcr Rflp ◽  
Molecular Selection ◽  
Lep Gene ◽  
Informative Content

The Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecular selection to improve carcass traits in beef cattle. This research was carried out to identify the genetic characterization of TG and LEP genes from 47 heads of Pasundan cows at West Java using PCR-RFLP method. Research shows that TG gene of Pasundan cattle is monomorphic with C allele as the dominant allele (1.00). However, LEP gene of Pasundan cattle is polymorphic with C allele as the dominant allele (0.98) and T as the rare allele (0.02). The polymorphic informative content (PIC) and numberof effective allele (ne) values in the LEP gene in the animal studied were 0.04 and 1.04 respectively. It was concluded that TG/BstYI and LEP/Sau3AI gene in the present study can not be used as molecular selection in Pasundan cattle. These results are important as the basic information for preparing the molecular selection program in the future.

scholarly journals Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia

2016 ◽  
Vol 10 (4) ◽  
pp. 438-444 ◽  
Author(s):  
Dwi Desmiyeni Putri ◽  
Ekowati Handharyani ◽  
Retno Damajanti Soejoedono ◽  
Agus Setiyono ◽  
Ni Luh Putu Ika Mayasari ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
West Java

Manganese removal from groundwater: characterization of filter media coating

2014 ◽  
Vol 55 (7) ◽  
pp. 1851-1863 ◽  
Author(s):  
Jantinus H. Bruins ◽  
Branislav Petrusevski ◽  
Yness M. Slokar ◽  
Joop C. Kruithof ◽  
Maria D. Kennedy
Keyword(s):  
Filter Media ◽  
Manganese Removal ◽  
Groundwater Characterization

scholarly journals Characterization of Bacteriophage Specific to Bacillus pumilus from Ciapus River in Bogor, West Java, Indonesia

2015 ◽  
Vol 22 (1) ◽  
pp. 27-33 ◽  
Author(s):  
ANIK KUSMIATUN ◽  
IMAN RUSMANA ◽  
SRI BUDIARTI
Keyword(s):  
Bacillus Pumilus ◽  
West Java

scholarly journals Characterization of Several Paddy Soil Types in Bogor, West Java, Indonesia

2017 ◽  
Vol 21 (1) ◽  
pp. 27
Author(s):  
. Kurniati ◽  
. Sudarsono ◽  
. Suwardi
Keyword(s):  
Bulk Density ◽  
Paddy Soil ◽  
Chemical Properties ◽  
Soil Types ◽  
West Java ◽  
Dry Land ◽  
Al Content ◽  
Fe And Mn ◽  
Density Values

Paddy soil has different morphology and pedogenic characteristics compared to dry land, due to the influence of inundation during several months in a year. Puddling and drying that occurs in turns (redox cycle) in paddy soil can lead to the formation of concretions or rusty Fe and Mn. The main purpose of this study was to understand the changing of the morphological and chemical properties as a result of changing of the dry land to paddy soil. Besides, the study also aimed to understand plow pan layer formation in Podsolic, Latosol, Regosol, and Andosol soil type. Results showed that content of soil density (bulk density) of dry land ranged from 0.5  to 1.0, while paddy soil is 0.8 to 1.0 (g cm-3).  Bulk density values in all four types of soils increased after the changing. Observation also demonstrated that severity levels of paddy soil is higher than dry land, especially in the second and third soil layers or under the surface of soils. Acidity of dry land was likely to be higher than paddy soil. There were no significant differences in nutrient such as C-organic, P and N. Meanwhile, using dithionite as solvent, paddy soil has higher Fe, Mn, and Al content than that of dry land, and remain the same when extracted with pyrophosphate and oxalate. From the four types of soil observed,the paddy soil showed formation of plow pan layer. This was shown by the soil severity level higher than the topsoil or other layers. Paddy soil had unique properties due to redox reaction, thereby providing soil discoloration i.e darker due to high solubility of Fe, Mn, and Al.Keywords: Concretion of Fe and Mn, paddy soil, pedogenesis, plow pan layer [How to Cite: Kurniati, Sudarsono and Suwardi. 2016. Characterization of Several Paddy Soil Types in Bogor, West Java, Indonesia . J Trop Soils 21:. 27-32. Doi: 10.5400/jts.2016.21.1.27]

scholarly journals Molecular Characterization of Begomovirus on Cucumber in Java

2019 ◽  
Vol 23 (2) ◽  
pp. 197
Author(s):  
Listihani Listihani ◽  
Tri Asmira Damayanti ◽  
Sri Hendrastuti Hidayat ◽  
Suryo Wiyono
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Amino Acid Sequences ◽  
Specific Primer ◽  
West Java ◽  
Central Java ◽  
Frequency Detection ◽  
Squash Leaf Curl Virus ◽  
Leaf Curl

A survey on several cucumber cultivation areas in West Java, Central Java, Yogyakarta, and East Java found many plants showing typical Begomovirus symptoms such as yellow mosaic, cupping, and vein banding. This study was aimed to determine disease frequency, detection and molecular characterization of the causal virus of those symptoms on cucumber in Java. Sampling was conducted by purposive sampling by collecting 50 symptomatic plants from each location in West Java (Indramayu, Subang, and Bogor), Central Java (Brebes and Klaten), Yogyakarta (Kulon Progo), and East Java (Nganjuk, Kediri, and Tulungagung). The detection and disease frequency was determined based on DIBA test using a specific antiserum of Tomato leaf curl New Delhi virus (ToLCNDV) and Squash leaf curl virus (SLCV). The identification of nucleic acid was conducted by PCR using specific primer of ToLCNDV and SLCV, DNA cloning, and sequencing. The results of serological detection showed the disease frequency of ToLCNDV and SLCV ranged from 92.77-100% and 78.33-93.3%, respectively. PCR using specific primer of ToLCNDV successfully amplified the coat protein gene at a size of 600 bp from all samples. Homology nucleotide and amino acid sequences among ToLCNDV Java isolate ranging from 95.6-99.2% and 99.7-100%. ToLCNDV isolates Java had highest nucleotide and amino acid sequences similarity with cucumber isolate from Klaten, Indonesia (AB613825) ranging from 96.1-98.1% and 99.7-100%, and was considered as “Indonesia” strain. SLCV not amplified on all samples by PCR using specific primer, indicating it might not present yet on cucumber in Java.

scholarly journals Identification and Characterization of Bacterial Lipase from Plateu Soil in West Java

2017 ◽  
Vol 18 (02) ◽  
pp. 103-108
Author(s):  
Vivitri Dewi Prasasty ◽  
Vinella Winata ◽  
Muhammad Hanafi
Keyword(s):  
Heat Stability ◽  
Industrial Applications ◽  
West Java ◽  
Ph Optimum ◽  
Good Efficiency ◽  
Optimum Ph ◽  
Glycerol Ester ◽  
Lipase Screening ◽  
Hydrolysis Of

Lipases are known as glycerol ester hydrolases that catalyze the hydrolysis of triglycerides into free fatty acids and glycerol. Lipases are found in human, animal, plant, and microorganisms. The aim of this research is to identify lipase producers and characterize bacterial lipase from West Java plateau soil. Plateau soil bacteria samples were isolated on lipase screening medium containing Rhodamine B. Olive oil was used as a substrate in screening and production medium bacterial lipases. From 16 bacterial isolate of lipase producers, 14 were identified as Bacillus sp. and the others were identified as Pseudomonas alcaligenes. All isolates were taken into production step to determine their lipase activities. Moreover, top 3 lipase activities out of 16 lipase activities were chosen to find the optimum pH and temperature. Both characterizations showed pH optimum and temperature optimum from each lipase. These optimum condition were used in heat stability characterization for each lipase samples. The result showed that lipase from isolate COK 2 in optimum pH 4 and temperature 50oC was the most stable lipase due to this sample has good and stable activity for 1 to 5 hours incubation time. Lipase sample from isolate COK 2 has good efficiency for lipase productivity in acid condition and high temperature. Results of this investigation could encourage utilization of these activity enhancers for various industrial applications.

scholarly journals Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia

2019 ◽  
Vol 20 (7) ◽  
Author(s):  
WINDA AYU SYAFITRI ◽  
FITRIA NINGSIH ◽  
PUTRI PRATIWI SETYANINGSIH ◽  
MAZYTHA KINANTI RACHMANIA ◽  
DHIAN CHITRA AYU FITRIA SARI ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Agar Plate ◽  
Amylolytic Activity ◽  
Phenotypic Characterization ◽  
Bootstrap Support ◽  
Rrna Gene ◽  
Geothermal Area ◽  
West Java

Abstract. Syafitri WA, Ningsih F, Setyaningsih PP, Rachmania MK, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridzal W. 2019. Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a geothermal area in West Java, Indonesia. Biodiversitas 20: 1929-1938. In this study, we describe the screening for amylolytic activity of 17 thermophilic Actinobacteria isolates obtained from the soil of Cisolok geysers, a geothermal area in West Java, Indonesia. All isolates were screened for amylolytic activity by the starch-agar plate method at various temperatures. The results showed that all of isolates were able to grow at 45oC. The growth abilities of the isolates grown in ISP 1 medium varied at temperatures from 45 to 60oC. Fifteen of the 17 isolates showed amylolytic activity at 45oC, 13 showed such activity at 50oC, and four showed activity at 55oC. Only three isolates, designated SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4, showed growth and amylolytic activity at 60oC. These three isolates were selected for molecular identification. The nearly full-length of 16S rRNA gene sequences data showed that these three isolates have a similarity of 99.93-100% with Actinomadura keratinilytica WCC-2265T and of 98.74-98.91% with A. miaoliensis BC 44T-5T. Phylogenetic tree shows that all three isolates are clustered together in a monophyletic group with the type strain of A. keratinilytica WCC-2265T as their most closely related species, with 100% bootstrap support. Based on sequencing of the 16S rRNA gene, phylogenetic comparison, and phenotypic characterization, the three isolates were identified as A. keratinilytica.

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