scholarly journals Extraction of extracellular polymeric substances of activated sludge and their application for wastewater treatment

Author(s):  
Zh Vasilieva ◽  
I Gaponenkov ◽  
M Vasekha ◽  
T Ivanova
2013 ◽  
Vol 773 ◽  
pp. 353-361
Author(s):  
Guang Jie Duan ◽  
Rui Jing Su ◽  
Deng Xin Li

Activated sludge process has been applied worldwide in municipal and industrial wastewater treatment practices. Excess sewage sludge, produced in sedimentation after wastewater bio-treatment, is an inevitable by-product of wastewater treatment processes. In any case sludge treatment and disposal should always be considered as an integral part of treatment of wastewater. Currently, the most widely available options in the world are the agriculture utilization, the waste disposal sites, the land reclamation and restoration, the incineration and other novel uses. Extracellular polymeric substances (EPS), as high-molecular weight compounds are secreted by activated sludge microorganisms, which can significantly influence the physicochemical properties and function of activated sludge flocs. Because of the crucial roles of EPS, many researchers have been made to explore their chemical compositions and physicochemical properties. Polysaccharides, humic substances and proteins are usually found to be the major components of EPS. The present paper sought to review past and future trends in sludge handling, focusing mainly at the organic matters.


Author(s):  
Rui Xu ◽  
Duanyi Huang ◽  
Xiaoxu Sun ◽  
Miaomiao Zhang ◽  
Dongbo Wang ◽  
...  

Biological arsenite (As(III)) oxidation is an important process in the removal of toxic arsenic (As) from contaminated water. However, the diversity and metabolic potentials of As(III)-oxidizing bacteria (AOBs) responsible for As(III) oxidation in wastewater treatment facilities are not well documented. In this study, two groups of bioreactors inoculated with activated sludge were operated under anoxic or oxic conditions to treat As-containing synthetic wastewater. Batch tests of inoculated sludges from the bioreactors further indicated that microorganisms could use nitrate or oxygen as electron acceptors to stimulate biological As(III) oxidation, suggesting the potentials of this process in wastewater treatment facilities. In addition, DNA-based stable isotope probing (DNA-SIP) was performed to identify the putative AOBs in the activated sludge. Bacteria associated with Thiobacillus were identified as nitrate-dependent AOBs, while bacteria associated with Hydrogenophaga were identified as aerobic AOBs in activated sludge. Metagenomic binning reconstructed a number of high-quality metagenome-assembled genomes (MAGs) associated with the putative AOBs. Functional genes encoding for As resistance, As(III) oxidation, denitrification, and carbon fixation were identified in these MAGs, suggesting their potentials for chemoautotrophic As(III) oxidation. In addition, the presence of genes encoding secondary metabolite biosynthesis and extracellular polymeric substance metabolism in these MAGs may facilitate the proliferation of these AOBs in activated sludge and enhance their capacity for As(III) oxidation. Importance AOBs play an important role in the removal of toxic arsenic from wastewater. Most of the AOBs have been isolated from natural environments. However, knowledge regarding the structure and functional roles of As(III)-oxidizing communities in wastewater treatment facilities are not well documented. The combination of DNA-SIP and metagenomic binning provides an opportunity to elucidate the diversity of in situ AOBs community inhabited the activated sludges. In this study, the putative AOBs responsible for As(III) oxidation in wastewater treatment facilities were identified, and their metabolic potentials including As(III) oxidation, denitrification, carbon fixation, secondary metabolites biosynthesis, and extracellular polymeric substances metabolisms were investigated. This observation provides an understanding of anoxic and/or oxic AOBs during the A(III) oxidation process in wastewater treatment facilities, which may contribute to the removal of As from contaminated water.


2012 ◽  
Vol 260-261 ◽  
pp. 1035-1040 ◽  
Author(s):  
Li Yun Ge ◽  
Huan Huan Deng ◽  
De Xiang Gao ◽  
Hong Wu Wang

Extracellular polymeric substances (EPS), which are vital cellular polymeric substances, play an important role in biological wastewater treatment. In this paper, four SBRs (blank, added 20mg/L Fe3+, added 40mg/L Cu2+, added both 20mg/L Fe3+ and 40mg/L Cu2+, respectively) were used to investigate the effect of Fe3+ and Cu2+ on EPS produced by microorganism from activated sludge. Carbohydrate and protein were main components of EPS. The total content of EPS, the content of protein and carbohydrate, and the content of DNA in EPS were detected. The results show metal ion had an effect on the formation of EPS and the total content of the EPS. The effect of ferric ion on protein was more obvious than copper ion. Furthermore, the addition of metal ion had little effect on DNA in EPS.


2016 ◽  
Vol 9 (2) ◽  
Author(s):  
Dinda Rita K. Hartaja ◽  
Imam Setiadi

Generally, wastewater of nata de coco industry contains suspended solids and COD were high, ranging from 90,000 mg / l. The high level of of the wastewater pollutants, resulting in nata de coco industry can not be directly disposed of its wastewater into the environment agency. Appropriate technology required in order to process the waste water so that the treated water can meet the environmental quality standards that are allowed. Designing the waste water treatment plant that is suitable and efficient for treating industrial wastewater nata de coco is the activated sludge process. Wastewater treatment using activated sludge process of conventional (standard) generally consists of initial sedimentation, aeration and final sedimentation.Keywords : Activated Sludge, Design, IPAL


1990 ◽  
Vol 22 (7-8) ◽  
pp. 131-138
Author(s):  
Ahmed Fadel

Many of Egypt's cities have existing treatment plants under operation that have been constructed before 1970. Almost all of these treatment plants now need rehabilitation and upgrading to extend their services for a longer period. One of these plants is the Beni Suef City Wastewater Treatment Plant. The Beni Suef WWTP was constructed in 1956. It has primary treatment followed by secondary treatment employing intermediate rate trickling filters. The BOD, COD, and SS concentration levels are relatively high. They are approximately 800, 1100, and 600 mg/litre, respectively. The Beni Suef city required the determination of the level of work needed for the rehabilitation and upgrading of the existing 200 l/s plant and to extend its capacity to 440 l/s at year 2000 A description of the existing units, their deficiencies and operation problems, and the required rehabilitation are presented and discussed in this paper. Major problems facing the upgrading were the lack of space for expansion and the shortage of funds. It was, therefore, necessary to study several alternative solutions and methods of treatment. The choice of alternatives was from one of the following schemes: a) changing the filter medium, its mode of operation and increasing the number of units, b) changing the trickling filter to high rate and combining it with the activated sludge process, for operation by one of several possible combinations such as: trickling filter-solids contact, roughing filter-activated sludge, and trickling filter-activated sludge process, c) dividing the flow into two parts, the first part to be treated using the existing system and the second part to be treated by activated sludge process, and d) expanding the existing system by increasing the numbers of the different process units. The selection of the alternative was based on technical, operational and economic evaluations. The different alternatives were compared on the basis of system costs, shock load handling, treatment plant operation and predicted effluent quality. The flow schemes for the alternatives are presented. The methodology of selecting the best alternative is discussed. From the study it was concluded that the first alternative is the most reliable from the point of view of costs, handling shock load, and operation.


1992 ◽  
Vol 25 (4-5) ◽  
pp. 203-209 ◽  
Author(s):  
R. Kayser ◽  
G. Stobbe ◽  
M. Werner

At Wolfsburg for a load of 100,000 p.e., the step-feed activated sludge process for nitrogen removal is successfully in operation. Due to the high denitrification potential (BOD:TKN = 5:1) the effluent total nitrogen content can be kept below 10 mg l−1 N; furthermore by some enhanced biological phosphate removal about 80% phosphorus may be removed without any chemicals.


1997 ◽  
Vol 36 (2-3) ◽  
pp. 1-8 ◽  
Author(s):  
P. Grau ◽  
B. P. Da-Rin

An unusually severe case of toxicity accompanied by activated sludge filamentous bulking was observed at the wastewater treatment plant Sao Paulo-Barueri. Treatment efficiency of the plant, operated without major problems for more than five years before, was significantly hindered for almost six months. Occurrence of toxic shocks was confirmed partly directly but mostly indirectly by inhibition of nitrification and biological phenomena related to toxicity. Several measures adopted, including the recycled activated sludge chlorination, are described in the paper.


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