Morphometric and genetic identification of a newly record pygmy seahorse Hippocampus denise in the Kepulauan Seribu reefs, Indonesia

Abstract The study was conducted to describe the seahorse species based on morphological and molecular characters. The pygmy seahorse in Panggang Island in Kepualuan Seribu was discovered in October 2011. The species was allegedly identified as Hippocampus denise (Family: Syngnathidae) described by Lourie and Randall which published in 2003. The high similarity is based on small morphometric, orange-like color and its association with sea fan Annella sp. Their habitat is fairly shallow at a depth between 13-24 meters compared with their sister species observed in Bali, Nusa Tenggara, and Sulawesi. The phylogenetic analysis constructed with several sequence data of Hippocampus spp. from Genbank shows that sample collected from Panggang Island is in the same clade with Hippocampus denise with 100% bootstrap value. BLAST analysis result also showed a high maximum similar identity (>99%) with the species Hippocampus denise. The seahorse specimen described in this study has a common typology of habitat with Hippocampus denise. This study shows that genetic analysis to determine the Hippocampus denise can be carried out to support species recognition, especially for cryptic species such as Hippocampus spp. There are variations in morphometric and habitat depth levels, indicating local adaptation of pygmy seahorses to the Kepulauan Seribu reefs.

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A new entomopathogenic fungus, Ophiocordyceps ponerus sp. nov., from China

Phytotaxa ◽

10.11646/phytotaxa.343.2.2 ◽

2018 ◽

Vol 343 (2) ◽

pp. 116 ◽

Cited By ~ 4

Author(s):

JIAOJIAO QU ◽

LAN QING YU ◽

JIAN ZHANG ◽

YANFENG HAN ◽

XIAO ZOU

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Entomopathogenic Fungus ◽

Sequence Data ◽

Systematic Position ◽

Similar Species ◽

Data Support ◽

A New Species ◽

Guiyang City ◽

Molecular Characters

A new species, Ophiocordyceps ponerus, is reported from a survey of invertebrate-associated fungi in Xiaochehe Wetland Park, Guiyang City, China. Evidence for the new species is provided by morphological and molecular characters. Synnemata of this species emerged from cadavers of soldier ants of Ponera sp. (Hymenoptera). It differs from similar species mainly in having verticillate phialides on the upper portions of the synnemata with septa, 112–158 × 7.5–10 μm; cylindrical or oval conidiogenous cells, which are inflated at the base, and suddenly tapering to a short, thin and verrucose neck, 13–25 × 2.5–5 μm; solitary, smooth conidia nearly oval or forming curved orange segments, 7.5–10 × 3.8–5 μm. Phylogenetic analysis using combined ITS, SSU, RPB2 and TEF sequence data support its systematic position in Ophiocordyceps and as a new species.

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The status of Myriangiaceae (Dothideomycetes)

Phytotaxa ◽

10.11646/phytotaxa.176.1.22 ◽

2014 ◽

Vol 176 (1) ◽

pp. 219 ◽

Cited By ~ 10

Author(s):

ASHA J. DISSANAYAKE ◽

RUVISHIKA S. JAYAWARDENA ◽

SARANYAPHAT BOONMEE ◽

KASUN M. THAMBUGALA ◽

QING TIAN ◽

...

Keyword(s):

Sequence Data ◽

Molecular Data ◽

Molecular Sequence Data ◽

Molecular Sequence ◽

The Family ◽

The Status ◽

Sexual State ◽

Morphological And Molecular Data ◽

Asexual State ◽

Molecular Characters

The family Myriangiaceae is relatively poorly known amongst the Dothideomycetes and includes genera which are saprobic, epiphytic and parasitic on the bark, leaves and branches of various plants. The family has not undergone any recent revision, however, molecular data has shown it to be a well-resolved family closely linked to Elsinoaceae in Myriangiales. Both morphological and molecular characters indicate that Elsinoaceae differs from Myriangiaceae. In Elsinoaceae, small numbers of asci form in locules in light coloured pseudostromata, which form typical scab-like blemishes on leaf or fruit surfaces. The coelomycetous, “Sphaceloma”-like asexual state of Elsinoaceae, form more frequently than the sexual state; conidiogenesis is phialidic and conidia are 1-celled and hyaline. In Myriangiaceae, locules with single asci are scattered in a superficial, coriaceous to sub-carbonaceous, black ascostromata and do not form scab-like blemishes. No asexual state is known. In this study, we revisit the family Myriangiaceae, and accept ten genera, providing descriptions and discussion on the generic types of Anhellia, Ascostratum, Butleria, Dictyocyclus, Diplotheca, Eurytheca, Hemimyriangium, Micularia, Myriangium and Zukaliopsis. The genera of Myriangiaceae are compared and contrasted. Myriangium duriaei is the type species of the family, while Diplotheca is similar and may possibly be congeneric. The placement of Anhellia in Myriangiaceae is supported by morphological and molecular data. Because of similarities with Myriangium, Ascostratum (A. insigne), Butleria (B. inaghatahani), Dictyocyclus (D. hydrangea), Eurytheca (E. trinitensis), Hemimyriangium (H. betulae), Micularia (M. merremiae) and Zukaliopsis (Z. amazonica) are placed in Myriangiaceae. Molecular sequence data from fresh collections is required to confirm the relationships and placement of the genera in this family.

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The genus Xylaria (Xylariaceae) in the south of China—6. A new Xylaria species based on morphological and molecular characters

Phytotaxa ◽

10.11646/phytotaxa.147.2.2 ◽

2013 ◽

Vol 147 (2) ◽

pp. 48 ◽

Cited By ~ 1

Author(s):

HAI-XIA MA ◽

LARISSA VASILYEVA ◽

YU LI

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Its Sequences ◽

Guizhou Province ◽

Nrdna Its ◽

Undescribed Species ◽

The South ◽

A New Species ◽

Molecular Characters

Xylaria fusispora, an undescribed species of Xylaria (Xylariales, Xylariaceae), is described and illustrated as a new species based on collections from Guizhou Province, China. Both morphology and phylogenetic analysis of nrDNA ITS sequences support the establishment of this new species. The fungus is characterized by its fusoid-equilateral ascospores and an ascus apical ring not bluing in Melzer’s reagent. The differences between the new species and the related fungi are discussed.

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Revised circumscription of Nothofagus and recognition of the segregate genera Fuscospora, Lophozonia, and Trisyngyne (Nothofagaceae)

Phytotaxa ◽

10.11646/phytotaxa.146.1.1 ◽

2013 ◽

Vol 146 (1) ◽

pp. 1 ◽

Cited By ~ 63

Author(s):

PETER B. HEENAN ◽

ROB D. SMISSEN

Keyword(s):

Phylogenetic Analysis ◽

South America ◽

Papua New Guinea ◽

New Genus ◽

New Caledonia ◽

Sequence Data ◽

Morphological Characters ◽

New Combinations ◽

Southern South America ◽

Dna Sequence Data

The generic taxonomy of the Nothofagaceae is revised. We present a new phylogenetic analysis of morphological characters and map these characters onto a recently published phylogenetic tree obtained from DNA sequence data. Results of these and previous analyses strongly support the monophyly of four clades of Nothofagaceae that are currently treated as subgenera of Nothofagus. The four clades of Nothofagaceae are robust and well-supported, with deep stem divergences, have evolutionary equivalence with other genera of Fagales, and can be circumscribed with morphological characters. We argue that these morphological and molecular differences are sufficient for the four clades of Nothofagaceae to be recognised at the primary rank of genus, and that this classification will be more informative and efficient than the currently circumscribed Nothofagus with four subgenera. Nothofagus is recircumscribed to include five species from southern South America, Lophozonia and Trisyngyne are reinstated, and the new genus Fuscospora is described. Fuscospora and Lophozonia, with six and seven species respectively, occur in New Zealand, southern South America and Australia. Trisyngyne comprises 25 species from New Caledonia, Papua New Guinea and Indonesia. New combinations are provided where necessary in each of these genera.

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Molecular and Ultrastructural Analysis of a Porocephalus sp. (Arthropoda: Pentastomida) Removed from a Rattlesnake

10.31753/jmas.66_101 ◽

2021 ◽

Vol Vol 66 (1) (January (1)) ◽

pp. 1-5

Author(s):

Jerome Goddard ◽

Gerald Baker ◽

Petra Jericke ◽

Lawrence Birchman ◽

Ethan Woodward ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Cytochrome C ◽

Cytochrome C Oxidase ◽

Adult Female ◽

Sequence Data ◽

Molecular Data ◽

Ultrastructural Analysis ◽

Oxidase Subunit ◽

Data Support ◽

Partial 18S

Ultrastructural and molecular data are provided from a single adult female pentastomid opportunistically collected from a road-killed rattlesnake in Russell, KS. Ultrastructural data consisted of light and SEM microscopy of the pentastomid and its eggs, while molecular data consisted of partial 18S and 28S ribosomal sequences and a partial cytochrome c oxidase subunit 1 sequence from the same specimen used for SEM. Ultrastructural and molecular data support generic identification of the pentastomid as Porocephalus sp. These molecular data were also used with previously published pentastomid sequence data for a concatenated phylogenetic analysis, which support the current, morphology-based taxonomic placement of the genus.

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Pseudodactylaria brevis sp. nov. from Thailand confirms the status of Pseudodactylariaceae

Phytotaxa ◽

10.11646/phytotaxa.369.4.1 ◽

2018 ◽

Vol 369 (4) ◽

pp. 241 ◽

Cited By ~ 1

Author(s):

CHUAN-GEN LIN ◽

ERIC H. C. MCKENZIE ◽

DARBHE J. BHAT ◽

JIAN-KUI LIU ◽

KEVIN D. HYDE ◽

...

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Sequence Data ◽

Taxonomic Status ◽

Its Sequence ◽

Morphological Comparison ◽

Phylogenetic Placement ◽

Data Analyses ◽

The Status ◽

A New Species

A new species, Pseudodactylaria brevis, is described, illustrated and compared with other Pseudodactylaria and Dactylaria-like taxa. Evidence for the new species is provided by morphological comparison and sequence data analyses. Pseudodactylaria brevis can be distinguished from other Pseudodactylaria and Dactylaria-like species by its short hyaline conidiophores and fusiform, 1-septate hyaline conidia. Phylogenetic analysis of LSU and ITS sequence data was carried out to determine the phylogenetic placement of the species and confirm the taxonomic status of Pseudodactylariaceae.

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Phylogenetic Analysis of a ‘Jewel Orchid’ Genus Goodyera (Orchidaceae) Based on DNA Sequence Data from Nuclear and Plastid Regions

PLoS ONE ◽

10.1371/journal.pone.0150366 ◽

2016 ◽

Vol 11 (2) ◽

pp. e0150366 ◽

Cited By ~ 6

Author(s):

Chao Hu ◽

Huaizhen Tian ◽

Hongqing Li ◽

Aiqun Hu ◽

Fuwu Xing ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Dna Sequence ◽

Sequence Data ◽

Dna Sequence Data ◽

Jewel Orchid

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Combined multi-gene backbone tree for the genus Coniochaeta with two new species from Uzbekistan

Phytotaxa ◽

10.11646/phytotaxa.336.1.3 ◽

2018 ◽

Vol 336 (1) ◽

pp. 43 ◽

Cited By ~ 5

Author(s):

MILAN C. SAMARAKOON ◽

YUSUFJON GAFFOROV ◽

NINGGUO LIU ◽

SAJEEWA S. N. MAHARACHCHIKUMBURA ◽

JAYARAMA D. BHAT ◽

...

Keyword(s):

New Species ◽

Sequence Data ◽

Species Recognition ◽

Phylogenetic Species ◽

Phylogenetic Studies ◽

Genealogical Concordance ◽

Two New Species ◽

Backbone Tree

The genus Coniochaeta is an important ascomycete because its members live in diversified habitats and nutritional modes. In this study, two new species, C. acaciae and C. coluteae, are introduced from dead branches of Acacia sp. and Colutea paulsenii Freyn (both Fabaceae) respectively from Uzbekistan, based on morphological and phylogenetic studies. Analyses of combined ITS and LSU sequence data with Genealogical Concordance Phylogenetic Species Recognition (GCPSR) and comparison of similar taxa, provide evidences for placement of these new species in Coniochaeta, as distinct lineages.

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Genetic identification of bovine leukaemia virus

Foods and raw materials ◽

10.21603/2308-4057-2018-2-314-324 ◽

2018 ◽

Vol 6 (2) ◽

pp. 314-324 ◽

Cited By ~ 3

Author(s):

Irina Donnik ◽

Ramil Vafin ◽

Aram Galstyan ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Research Methods ◽

Bovine Leukemia Virus ◽

Leukemia Virus ◽

Molecular Genetic ◽

Genetic Research ◽

Genetic Identification ◽

Gene Fragment ◽

Phylogenetic Classification ◽

Pcr Rflp

Molecular genetic research methods make it possible to evaluate the genetic diversity of bovine leukemia virus (BLV) and are the most informative approaches to its genetic identification. Molecular genetic research methods work well for the phylogenetic analysis of sequenced nucleotide DNA sequences of the provirus, as well as for the polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) according to the phylogenetic classification of the pathogen. The purpose of the research was to study the scientific and methodological approaches to the genetic identification of bovine leukemia virus, integrated into the molecular monitoring of infection of cattle with BLV genotypes. The authors used PCR-RFLP-genotyping and comparative phylogenetic analysis of aligned nucleotide sequences of the env gene fragment of the BLV provirus isolates to detect the genotypic affiliation of the cattle from twenty-one livestock farms of the Republic of Tatarstan. As a result, isolates of four out of ten BLV genotypes were found in the Tatarstani cattle, namely genotypes 1, 4, 7, and 8. The research involved a comparative analysis of 505 nucleotide sequences of a fragment of the BLV env gene, including those deposited in GenBank NCBI. The analysis confirms the inconsistency of several earlier PCR-RFLP typing strategies with the current approach in assessing the genotypic diversity by phylogenetic analysis. The improved strategy of PCR-RFLP genotyping of BLV corresponds with its modern phylogenetic classification. The strategy makes it possible to identify all the known genotypes of the viral pathogen. Its validity has been proved by in silico modelling of restrictogrammes and a phylogenetic analysis of the env gene fragment of 57 reference isolates of ten BLV genotypes that generate 57 genotype-associated combinations of diagnostically significant PCR-RFLP profiles.

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