scholarly journals Active Fraction as Anti-obesity by in Vitro toward Pancreatic Lipase Activity

2021 ◽  
Vol 1070 (1) ◽  
pp. 012012
Author(s):  
D Iswantini ◽  
N Hanif ◽  
D RizkiPranoto ◽  
Trivadila
Keyword(s):  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Active Fraction

scholarly journals Caffeic Acid Derivatives from Bacopa monniera Plants as Inhibitors of Pancreatic Lipase Activity and their Structural Requirements

2016 ◽  
Vol 11 (12) ◽  
pp. 1934578X1601101
Author(s):  
Souichi Nakashima ◽  
Tomoe Ohta ◽  
Seikou Nakamura ◽  
Yoshimi Oda ◽  
Mari Koumoto ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Methanol Extract ◽  
Bacopa Monniera ◽  
Inhibitory Effects ◽  
Active Constituent ◽  
Structural Requirements ◽  
Phenylpropanoid Glycosides ◽  
First Time

The methanol extract of whole Bacopa monniera plants inhibited pancreatic lipase activity in vitro. From this extract we have reported the isolation of 11 triterpene glycosides and 5 phenylethanoid- and/or phenylpropanoid- glycosides. In this paper, we describe the effects of the methanol extract and/or its constituents on pancreatic lipase activity and the isolation of an active constituent, desrhamnosyl isoacteoside. In addition, the structural requirements for its inhibitory effects were examined. We also examined the effects on the elevation of plasma triglyceride (TG) levels in olive oil loaded mice. The major active constituents, desrhamnosyl isoacteoside and plantainoside B, reduced plasma TG levels in the mice. The inhibitory effects of B. monniera and its constituents on pancreatic lipase activity and plasma TG level are reported for the first time.

scholarly journals Inhibitory Activities of Polyphenolic Extracts of Bangladeshi Vegetables against α-Amylase, α-Glucosidase, Pancreatic Lipase, Renin, and Angiotensin-Converting Enzyme

Foods ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 844
Author(s):  
Razia Sultana ◽  
Adeola M. Alashi ◽  
Khaleda Islam ◽  
Md Saifullah ◽  
C. Emdad Haque ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Inhibitory Activity ◽  
The Other ◽  
Converting Enzyme ◽  
Inhibitory Activities ◽  
Ash Gourd ◽  
Better Than

The aim of the study was to determine the in vitro enzyme inhibition activities of aqueous polyphenolic extracts of nine popular Bangladeshi vegetables, namely ash gourd, bitter gourd, brinjal, Indian spinach, kangkong, okra, ridge gourd, snake gourd, and stem amaranth. Polyphenolic glycosides were the major compounds present in the extracts. Inhibition of α-amylase (up to 100% at 1 mg/mL) was stronger than α-glucosidase inhibition (up to 70.78% at 10 mg/mL). The Indian spinach extract was the strongest inhibitor of pancreatic lipase activity (IC50 = 276.77 µg/mL), which was significantly better than that of orlistat (381.16 µg/mL), a drug. Ash gourd (76.51%), brinjal (72.48%), and snake gourd (66.82%) extracts were the most effective inhibitors of angiotensin-converting enzyme (ACE), an enzyme whose excessive activities have been associated with hypertension. Brinjal also had a significantly higher renin-inhibitory activity than the other vegetable extracts. We conclude that the vegetable extracts may have the ability to reduce enzyme activities that have been associated with hyperglycemia, hyperlipidemia, and hypertension.

scholarly journals Inhibitory Activity of Benzophenones from Anemarrhena asphodeloides on Pancreatic Lipase

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Yang Hee Jo ◽  
Seon Beom Kim ◽  
Jong Hoon Ahn ◽  
Qing Liu ◽  
Bang Yeon Hwang ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Soluble Fraction ◽  
Significant Inhibition ◽  
Dietary Fats ◽  
Lipid Absorption ◽  
Vitro Assay ◽  
Key Enzyme ◽  
Anemarrhena Asphodeloides

Pancreatic lipase is a key enzyme for lipid absorption by hydrolysis of total dietary fats. Therefore, inhibition of pancreatic lipase is suggested to be an effective therapy in the regulation of obesity. The EtOAc-soluble fraction of Anemarrhena asphodeloides rhizomes significantly inhibited pancreatic lipase activity as assessed using porcine pancreatic lipase as an in vitro assay system. Further fractionation of the EtOAc-soluble fraction of A. asphodeloides led to the isolation of a new benzophenone glycoside, zimoside A (1), together with the eleven known compounds iriflophenone (2), 2,4′,6-trihydroxy-4-methoxybenzophenone (3), foliamangiferoside A (4), (2,3-dihydroxy-4-methoxyphenyl)(4-hydroxyphenyl)-methanone (5), 1,4,5,6,-tetrahydroxyxanthone (6), isosakuranetin (7), 4-hydroxybenzoic acid (8), 4-hydroxyacetophenone (9), vanillic acid (10), tyrosol (11) and 5-hydroxymethyl-2-furaldehyde (12). Among the isolated compounds, 3, 5 and 10 showed significant inhibition of pancreatic lipase activity.

scholarly journals The Effects of Cordyceps sinensis (Berk.) Sacc. and Gymnema inodorum (Lour.) Decne. Extracts on Adipogenesis and Lipase Activity In Vitro

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Kanokwan Tiamyom ◽  
Kittipot Sirichaiwetchakoon ◽  
Tanaporn Hengpratom ◽  
Sajeera Kupittayanant ◽  
Rungrudee Srisawat ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Lipid Accumulation ◽  
Acyl Chain ◽  
Principal Component ◽  
Cordyceps Sinensis ◽  
Dependent Manner ◽  
Inhibitory Effects ◽  
Ftir Microspectroscopy

This study aimed to investigate the effects of Cordyceps sinensis extract (CSE) and Gymnema inodorum extract (GIE), used alone and combined, on antiadipogenesis in 3T3-L1 cells. Oil Red O staining was used to examine the effects of these extracts on inhibition of intracellular lipid accumulation in 3T3-L1 adipocytes and on lipid droplet morphology. Fourier transform-infrared (FTIR) microspectroscopy was used to examine biomolecular changes in 3T3-L1 adipocytes. The pancreatic lipase assay was used to evaluate the inhibitory effects of CSE and GIE on pancreatic lipase activity. Taken together, the results indicated that CSE, GIE, and their combination suppressed lipid accumulation. The FTIR microspectroscopy results indicated that CSE, GIE, and their combination had inhibitory effects on lipid accumulation in the adipocytes. Compared with the untreated adipocytes, the signal intensity and integrated areas of glycogen and other carbohydrates, the acyl chain of phospholipids, and the lipid/protein ratios of the CSE, GIE, alone, and combined treated adipocytes were significantly lower (p < 0.05). Combination treatment resulted in a synergistic effect on lipid accumulation reduction in the adipocytes. Principal component analysis of the biomolecular changes revealed six distinct clusters in the FTIR spectra of the sample cells. The pancreatic lipase assay results indicated that CSE and GIE inhibited the pancreatic lipase activity in a dose-dependent manner (mean ± standard error of the mean IC50 values, 2312.44 ± 176.55 μg mL−1 and 982.24 ± 44.40 μg mL−1, resp.). Our findings indicated that FTIR microspectroscopy has potential application for evaluation of the effectiveness of medicinal plants and for the development of infrared biochemical obesity markers useful for treating patients with obesity. These results suggested that use of CSE and GIE alone and in combination may be efficacious as a complementary therapy for hyperlipidemia and obesity management. However, clinical trials in animals and humans must first be completed.

scholarly journals Maté Tea Inhibits In Vitro Pancreatic Lipase Activity and Has Hypolipidemic Effect on High-fat Diet-induced Obese Mice

Obesity ◽  
2010 ◽  
Vol 18 (1) ◽  
pp. 42-47 ◽  
Author(s):  
Fernanda Martins ◽  
Tatiana M. Noso ◽  
Viviane B. Porto ◽  
Alline Curiel ◽  
Alessandra Gambero ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Lipase Activity ◽  
High Fat Diet ◽  
Hypolipidemic Effect ◽  
Obese Mice ◽  
High Fat ◽  
Mate Tea

scholarly journals Testing edible mushrooms to inhibit the pancreatic lipase activity by an in vitro digestion model

2012 ◽  
Vol 47 (5) ◽  
pp. 1004-1010 ◽  
Author(s):  
Marimuthu Palanisamy ◽  
Alicia Gil-Ramírez ◽  
Alejandro Ruiz-Rodríguez ◽  
Francisco R. Marín ◽  
Guillermo Reglero ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Lipase Activity ◽  
In Vitro Digestion ◽  
Edible Mushrooms

Effect of Lipid Excipients on In Vitro Pancreatic Lipase Activity

2003 ◽  
Vol 29 (8) ◽  
pp. 885-890 ◽  
Author(s):  
Ramaswamy Subramanian ◽  
Kishor M. Wasan
Keyword(s):  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Lipid Excipients

PANCREATIC LIPASE ACTIVITY IN RELATION TO HIGH LEVELS OF FAT IN CALF MILK REPLACERS

1979 ◽  
Vol 59 (2) ◽  
pp. 463-465
Author(s):  
K. J. JENKINS
Keyword(s):  
Small Intestine ◽  
Pancreatic Lipase ◽  
Lipase Activity ◽  
In Vitro Study ◽  
Low Ph ◽  
Milk Replacer ◽  
High Fat ◽  
Ph Values ◽  
Milk Replacers

An in vitro study indicated that pancreatic lipase (PL) can effectively hydrolyze and solubilize milk replacer fats under conditions simulating those in the small intestine. When PL was added to high fat milk replacers very little lipolysis occurred in vitro at the low pH values encountered in the calf abomasum.

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