scholarly journals Start-up and Nitrogen Removal Performance of DAMOanammox Inoculated with Anaerobic Granular Sludge in MBfR

Author(s):  
Xiaoting Guo ◽  
Ying Shi ◽  
Xiaojing Yao
2012 ◽  
Vol 518-523 ◽  
pp. 2391-2398
Author(s):  
Yan He ◽  
Gong Ming Zhou ◽  
Min Sheng Huang ◽  
Min Tong

Three kinds of seeding sludge, i.e. conventional activated sludge, anaerobic granular sludge and the nitrifying activated sludge from the nitritation reactor treating aged leachates were evaluated in batch mode to screen the optimized inoculum for the rapid start-up of ANAMMOX reactor. The feasibility of the ANAMMOX process for the treatment of aged leachates was also investigated in a modified upflow anaerobic sludge blanket (UASB, 0.05m3). The batch experiments revealed that the nitrifying activated sludge from the nitritation reactor could respectively achieve the NRR (nitrogen removal rate) of 0.0365 kg N/(m3.d) and the ARR (ammonium removal rate) of 0.013 kg N/(m3.d) on day 12, which were greatly higher than those of the other two tested sludge samples. The mixture of the aforementioned nitrifying activated sludge and anaerobic granular sludge was established as an effective inoculum for the prompt start-up of ANAMMOX reactor. The maximum total nitrogen removal rate of 0.826 kg N/(m3.d) could be obtained for the treatment of “old” leachates under NLR (nitrogen loading rate) of 1.028 kg N/(m3.d). It is concluded that the N-removal performance of ANAMMOX process is still to be improved for actual engineering application to aged landfill leachates.


2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Xiaoling Zhang ◽  
Fan Zhang ◽  
Yanhong Zhao ◽  
Zhengqun Li

The start-up and performance of the completely autotrophic nitrogen removal via nitrite (CANON) process were examined in a sequencing batch reactor (SBR) with intermittent aeration. Initially, partial nitrification was established, and then the DO concentration was lowered further, surplus water in the SBR with high nitrite was replaced with tap water, and continuous aeration mode was turned into intermittent aeration mode, while the removal of total nitrogen was still weak. However, the total nitrogen (TN) removal efficiency and nitrogen removal loading reached 83.07% and 0.422 kgN/(m3·d), respectively, 14 days after inoculating 0.15 g of CANON biofilm biomass into the SBR. The aggregates formed in SBR were the mixture of activated sludge and granular sludge; the volume ratio of floc and granular sludge was 7 : 3. DNA analysis showed that Planctomycetes-like anammox bacteria and Nitrosomonas-like aerobic ammonium oxidization bacteria were dominant bacteria in the reactor. The influence of aeration strategies on CANON process was investigated using batch tests. The result showed that the strategy of alternating aeration (1 h) and nonaeration (1 h) was optimum, which can obtain almost the same TN removal efficiency as continuous aeration while reducing the energy consumption, inhibiting the activity of NOB, and enhancing the activity of AAOB.


2004 ◽  
Vol 50 (6) ◽  
pp. 207-215 ◽  
Author(s):  
Y.-H. Ahn ◽  
H.-C. Kim

The sustainable anaerobic nitrogen removal and microbial granulation were investigated by using a laboratory anaerobic granular sludge bed reactor, treating synthetic (inorganic and organic) wastewater and piggery waste. From inorganic synthetic wastewater, lithoautotrophic ammonium oxidation to nitrite/nitrate was observed by an addition of hydroxylamine. Also, the results revealed that the Anammox intermediates (particularly, hydrazine) contents in the substrate would be one of the important parameters for success of the anaerobic nitrogen removal process. The results from organic synthetic wastewater show that if the Anammox organism were not great enough in the startup of the process, denitritation and anaerobic ammonification would be a process prior to the Anammox reaction. The anaerobic ammonium removal from the piggery waste was performed successfully, probably due to the Anammox intermediates contained in the substrate. This reactor shows a complex performance including the Anammox reaction and HAP crystallization, as well as having partial denitritation occurring simultaneously. From the activity test, the maximum specific N conversion rate was 0.1 g NH4-N/g VSS/day (0.77 g T-N/g VSS/day), indicating that potential denitritation is quite high. The NO2-N/NH4-N ratio to Anammox is 1.17. The colour of the biomass treating the piggery waste changed from black to dark red. It was also observed that the red-colored granular sludge had a diameter of 1-2 mm. The settleability assessment of the granular sludge revealed that the granular sludge had a good settleability even though it was worse than that of seed granular sludge.


2014 ◽  
Vol 675-677 ◽  
pp. 633-637
Author(s):  
Ze Ya Wang ◽  
Li Ping Qiu ◽  
Li Xin Zhang ◽  
Jia Bin Wang

A set of bench scale ASBR reactors with 0.5 L effective volume were carried out to culture anaerobic ammonia oxidizing bacteria, while the anaerobic granular sludge was inoculated into these reactors as well as the operating temperature is 30±1°C, HRT is 72h and pH is approximate 7.8 in this experiment. After 60 days running, these reactors appeared anaerobic ammonia oxidation phenomenon. When the influent NH4+-N and NO2--N concentrations were approximately 50 mg/L and 70 mg/L, the NH4+-N, NO2--N and TN removal were 80%, 90% and 70%, respectively, the ratio of the NH4+-N and NO2--N removal and NO3--N production is approximately 1:1.5:0.25, close to the theoretical valve of 1:1.32:0.26 and that mainly accord with the chemical equilibrium of anaerobic ammonia oxidation mode. Furthermore, when the phenomenon of anaerobic ammonia oxidation appeared, effluent pH value was slightly higher than influent and the sludge become red.


2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Lei Xiong ◽  
Yun-Yan Wang ◽  
Chong-Jian Tang ◽  
Li-Yuan Chai ◽  
Kang-Que Xu ◽  
...  

The granulation of anammox sludge plays an important role in the high nitrogen removal performance of the anammox reactor. In this study, anaerobic granular sludge was selected as the seeding sludge to start up anammox reactor in order to directly obtain anammox granules. Results showed that the anammox UASB reactor was successfully started up by inoculating anaerobic granular sludge, with substrate capacity of 4435.2 mg/(L·d) and average ammonium and nitrite removal efficiency of 90.36% and 93.29%, respectively. During the start-up course, the granular sludge initially disintegrated and then reaggregated and turned red, suggesting the high anammox performance. Zn-Fe precipitation was observed on the surface of granules during the operation by SEM-EDS, which would impose inhibition to the anammox activity of the granules. Accordingly, it is suggested to relatively reduce the trace metals concentrations, of Fe and Zn in the conventional medium. The findings of this study are expected to be used for a shorter start-up and more stable operation of anammox system.


2011 ◽  
Vol 63 (9) ◽  
pp. 1808-1814 ◽  
Author(s):  
M. Figueroa ◽  
A. Val del Río ◽  
J. L. Campos ◽  
A. Mosquera-Corral ◽  
R. Méndez

Aerobic granular sludge grown in a sequential batch reactor was proposed as an alternative to anaerobic processes for organic matter and nitrogen removal from swine slurry. Aerobic granulation was achieved with this wastewater after few days from start-up. On day 140 of operation, the granular properties were: 5 mm of average diameter, SVI of 32 mL (g VSS)−1 and density around 55 g VSS (Lgranule)−1. Organic matter removal efficiencies up to 87% and nitrogen removal efficiencies up to 70% were achieved during the treatment of organic and nitrogen loading rates (OLR and NLR) of 4.4 kg COD m−3 d−1 and of 0.83 kg N m−3 d−1, respectively. However, nitrogen removal processes were negatively affected when applied OLR was 7.0 kg COD m−3 d−1 and NLR was 1.26 kg N m−3 d−1. The operational cycle of the reactor was modified by reducing the volumetric exchange ratio from 50 to 6% in order to be able to treat the raw slurry without dilution.


2015 ◽  
Vol 71 (7) ◽  
pp. 1040-1046 ◽  
Author(s):  
J. Wagner ◽  
L. B. Guimarães ◽  
T. R. V. Akaboci ◽  
R. H. R. Costa

This study evaluated aerobic granulation and nitrogen removal via assimilation, nitrification, and denitrification of a system fed with real domestic wastewater. The granulation process was complete after 160 days of operation. The mature granules had an almost spherical structure, an average size of 473.0 μm, and a good settling ability (SVI30 of 75.6 mL g−1). Ammonium assimilation for cell growth varied between 3.5 and 64.6% during reactor start-up. After granule formation, assimilation accounted for less than 5% and nitrogen was mainly removed by partial nitrification up to nitrite, followed by denitrification via nitrite. Average efficiencies of 86.6% for nitrification, 59.5% for denitrification, and 60.5% for total nitrogen were obtained in this period. The assimilation ability of the mature granules grown on domestic wastewater was lower than the commonly reported results obtained for synthetic granules.


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