Anaerobic Ammonia Oxidation in Start-Up of Bench Scale ASBR

2014 ◽  
Vol 675-677 ◽  
pp. 633-637
Author(s):  
Ze Ya Wang ◽  
Li Ping Qiu ◽  
Li Xin Zhang ◽  
Jia Bin Wang
Keyword(s):  
Ammonia Oxidation ◽  
Ph Value ◽  
Operating Temperature ◽  
Granular Sludge ◽  
Anaerobic Granular Sludge ◽  
Ammonia Oxidizing Bacteria ◽  
Bench Scale ◽  
N Removal ◽  
Start Up ◽  
Anaerobic Ammonia Oxidation

A set of bench scale ASBR reactors with 0.5 L effective volume were carried out to culture anaerobic ammonia oxidizing bacteria, while the anaerobic granular sludge was inoculated into these reactors as well as the operating temperature is 30±1°C, HRT is 72h and pH is approximate 7.8 in this experiment. After 60 days running, these reactors appeared anaerobic ammonia oxidation phenomenon. When the influent NH4+-N and NO2--N concentrations were approximately 50 mg/L and 70 mg/L, the NH4+-N, NO2--N and TN removal were 80%, 90% and 70%, respectively, the ratio of the NH4+-N and NO2--N removal and NO3--N production is approximately 1:1.5:0.25, close to the theoretical valve of 1:1.32:0.26 and that mainly accord with the chemical equilibrium of anaerobic ammonia oxidation mode. Furthermore, when the phenomenon of anaerobic ammonia oxidation appeared, effluent pH value was slightly higher than influent and the sludge become red.

Assessment of Inocula and N-Removal Performance of Anaerobic Ammonium Oxidation (ANAMMOX) for the Treatment of Aged Landfill Leachates

2012 ◽  
Vol 518-523 ◽  
pp. 2391-2398
Author(s):  
Yan He ◽  
Gong Ming Zhou ◽  
Min Sheng Huang ◽  
Min Tong
Keyword(s):  
Activated Sludge ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Granular Sludge ◽  
Nitrogen Loading ◽  
Anaerobic Granular Sludge ◽  
Landfill Leachates ◽  
N Removal ◽  
Start Up ◽  
Anammox Process

Three kinds of seeding sludge, i.e. conventional activated sludge, anaerobic granular sludge and the nitrifying activated sludge from the nitritation reactor treating aged leachates were evaluated in batch mode to screen the optimized inoculum for the rapid start-up of ANAMMOX reactor. The feasibility of the ANAMMOX process for the treatment of aged leachates was also investigated in a modified upflow anaerobic sludge blanket (UASB, 0.05m3). The batch experiments revealed that the nitrifying activated sludge from the nitritation reactor could respectively achieve the NRR (nitrogen removal rate) of 0.0365 kg N/(m3.d) and the ARR (ammonium removal rate) of 0.013 kg N/(m3.d) on day 12, which were greatly higher than those of the other two tested sludge samples. The mixture of the aforementioned nitrifying activated sludge and anaerobic granular sludge was established as an effective inoculum for the prompt start-up of ANAMMOX reactor. The maximum total nitrogen removal rate of 0.826 kg N/(m3.d) could be obtained for the treatment of “old” leachates under NLR (nitrogen loading rate) of 1.028 kg N/(m3.d). It is concluded that the N-removal performance of ANAMMOX process is still to be improved for actual engineering application to aged landfill leachates.

Acid Endurance and Sorption of Zn2+ of Anaerobic Granular Sludge Acclimated by Flue Gas Pretreatment Wastewater

2013 ◽  
Vol 634-638 ◽  
pp. 182-186
Author(s):  
Juan Wang ◽  
Qin Zhong
Keyword(s):  
Flue Gas ◽  
Ph Value ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Anaerobic Granular Sludge ◽  
Methanogenic Activity ◽  
Activity Inhibition ◽  
Anaerobic Sludge Blanket

With the aim to use anaerobic granular sludge, the methanogenic activity inhibition and recovery of anaerobic granular sludge from an industrial anaerobic reactor (s1) were investigated by measuring the methane volume at low pH. A lab-scale upflow anaerobic sludge blanket (UASB) reactor was inoculated with s1.s1 was used to remove Zn2+ in wastewater. The results show that activity of s1 is similar when the pH value is 6.5 to 7.0. The methane volume is obviously decreased when the pH value is 6.0. The activity is completely inhibited when the pH value is 4.5. The activity is fully recovered when the pH is above 6.5 and hardly recovers when the pH fell to 4.5. The main Zn2+ removal mechanism is chemical adsorption.

Rapid start-up of a nitrifying reactor using aerobic granular sludge as seed sludge

2012 ◽  
Vol 65 (3) ◽  
pp. 581-588 ◽  
Author(s):  
Naohiro Kishida ◽  
Goro Saeki ◽  
Satoshi Tsuneda ◽  
Ryuichi Sudo
Keyword(s):  
Removal Rate ◽  
Granular Sludge ◽  
Ammonia Removal ◽  
High Rate ◽  
Aerobic Granular Sludge ◽  
Nitrite Accumulation ◽  
Ammonia Oxidizing Bacteria ◽  
Continuous Stirred Tank Reactor ◽  
Start Up ◽  
Seed Sludge

In this study, the effectiveness of aerobic granular sludge as seed sludge for rapid start-up of nitrifying processes was investigated using a laboratory-scale continuous stirred-tank reactor (CSTR) fed with completely inorganic wastewater which contained a high concentration of ammonia. Even when a large amount of granular biomass was inoculated in the reactor, and the characteristics of influent wastewater were abruptly changed, excess biomass washout was not observed, and biomass concentration was kept high at the start-up period due to high settling ability of the aerobic granular sludge. As a result, an ammonia removal rate immediately increased and reached more than 1.0 kg N/m3/d within 20 days and up to 1.8 kg N/m3/d on day 39. Subsequently, high rate nitritation was stably attained during 100 days. However, nitrite accumulation had been observed for 140 days before attaining complete nitrification to nitrate. Fluorescence in situ hybridization analysis revealed the increase in amount of ammonia-oxidizing bacteria which existed in the outer edge of the granular sludge during the start-up period. This microbial ecological change would make it possible to attain high rate ammonia removal.

scholarly journals Simultaneous nitrification and phosphate removal by bioaugmented aerobic granules treating a fluoroorganic compound

2021 ◽  
Author(s):  
Anouk F. Duque ◽  
Vânia S. Bessa ◽  
Udo van Dongen ◽  
Merle K. de Kreuk ◽  
Raquel B. R. Mesquita ◽  
...  
Keyword(s):  
Denaturing Gradient Gel Electrophoresis ◽  
Amoa Gene ◽  
Granular Sludge ◽  
Phosphate Removal ◽  
Aerobic Granular Sludge ◽  
Ammonia Oxidizing Bacteria ◽  
Aerobic Granules ◽  
N Removal ◽  
Nitrite Oxidizing Bacteria ◽  
Gradient Gel Electrophoresis

Abstract The presence of toxic compounds in wastewater can cause problems for organic matter and nutrient removal. In this study, the long term effect of a model xenobiotic, 2-fluorophenol (2-FP), on ammonia oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB) and phosphate accumulating organisms (PAO) in aerobic granular sludge was investigated. Phosphate (P) and ammonium (N) removal efficiencies were high (>93%) and, after bioaugmentation with 2-FP degrading strain FP1, 2-FP was completely degraded. Neither N nor P removal were affected by 50 mg L−1 of 2-FP in the feed stream. Changes in the aerobic granule bacterial communities were followed. Numerical analysis of the denaturing gradient gel electrophoresis (DGGE) profiles showed low diversity for the amoA gene with an even distribution of species. PAOs, including denitrifying PAO (dPAO), and AOB were present in the 2-FP degrading granules, although dPAO population decreased throughout the 444 days reactor operation. The results demonstrated that the aerobic granules bioaugmented with FP1 strain successfully removed N, P and 2-FP simultaneously.

Research on Features of Domestic Wastewater Treatment and Mechanism of Denitrification in Anaerobic MBBR Reactor

2012 ◽  
Vol 455-456 ◽  
pp. 1030-1036
Author(s):  
Hui Zhou Yuan ◽  
Jian Bang Zhao ◽  
Shui Zhou Ke
Keyword(s):  
Wastewater Treatment ◽  
Retention Time ◽  
Hydraulic Retention Time ◽  
Ammonia Oxidation ◽  
Ph Value ◽  
Domestic Wastewater ◽  
N Concentration ◽  
Domestic Wastewater Treatment ◽  
Anaerobic Ammonia Oxidation ◽  
Nitrification And Denitrification

This paper studied on the treatment of urban domestic wastewater with Anaerobic MBBR in the tropical and subtropical areas in the South of China. The emphasis focused on the effects of the Hydraulic Retention Time (HRT) and pH value to the CODCrconcentration and the NH3-N concentration of the outflow of the reactor. Moreover, the mechanism of denitrification was also explored. The results showed that the mechanism of denitrification of the reactor was found. When HRT was equal to or more than 8h, NH3-N was reduced mainly in the way of short-cut nitrification and denitrification. When HRT was less than 8h, the reactions of short-cut nitrification and denitrification & anaerobic ammonia oxidation both existed in the reactor.

scholarly journals Start-Up Characteristics of a Granule-Based Anammox UASB Reactor Seeded with Anaerobic Granular Sludge

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Lei Xiong ◽  
Yun-Yan Wang ◽  
Chong-Jian Tang ◽  
Li-Yuan Chai ◽  
Kang-Que Xu ◽  
...  
Keyword(s):  
Trace Metals ◽  
Granular Sludge ◽  
Uasb Reactor ◽  
Stable Operation ◽  
Anaerobic Granular Sludge ◽  
High Nitrogen ◽  
Start Up ◽  
Anammox Activity ◽  
Nitrite Removal ◽  
Conventional Medium

The granulation of anammox sludge plays an important role in the high nitrogen removal performance of the anammox reactor. In this study, anaerobic granular sludge was selected as the seeding sludge to start up anammox reactor in order to directly obtain anammox granules. Results showed that the anammox UASB reactor was successfully started up by inoculating anaerobic granular sludge, with substrate capacity of 4435.2 mg/(L·d) and average ammonium and nitrite removal efficiency of 90.36% and 93.29%, respectively. During the start-up course, the granular sludge initially disintegrated and then reaggregated and turned red, suggesting the high anammox performance. Zn-Fe precipitation was observed on the surface of granules during the operation by SEM-EDS, which would impose inhibition to the anammox activity of the granules. Accordingly, it is suggested to relatively reduce the trace metals concentrations, of Fe and Zn in the conventional medium. The findings of this study are expected to be used for a shorter start-up and more stable operation of anammox system.

scholarly journals Performance and microbial community of the CANON process in a sequencing batch membrane bioreactor with elevated COD/N ratios

2020 ◽  
Vol 81 (1) ◽  
pp. 138-147
Author(s):  
Xiaoling Zhang ◽  
Xincong Liu ◽  
Meng Zhang
Keyword(s):  
Organic Matter ◽  
Microbial Community ◽  
Nitrogen Removal ◽  
Membrane Bioreactor ◽  
Ammonia Oxidation ◽  
Heterotrophic Bacteria ◽  
Removal Rate ◽  
Ammonia Oxidizing Bacteria ◽  
Anaerobic Ammonia Oxidation ◽  
Canon Process

Abstract In this study, the effects of elevated chemical oxygen demand/nitrogen (COD/N) ratios on nitrogen removal, production and composition of the extracellular polymer substances (EPS) and microbial community of a completely autotrophic nitrogen removal via nitrite (CANON) process were studied in a sequencing batch membrane bioreactor (SBMBR). The whole experiment was divided into two stages: the CANON stage (without organic matter in influent) and the simultaneous partial nitrification, anaerobic ammonia oxidation and denitrification (SNAD) stage (with organic matter in influent). When the inflow ammonia nitrogen was 420 mg/L and the COD/N ratio was no higher than 0.8, the addition of COD was helpful to the CANON process; the total nitrogen removal efficiency (TNE) was improved from approximately 65% to more than 75%, and the nitrogen removal rate (NRR) was improved from approximately 0.255 kgN/(m3·d) to approximately 0.278 kgN/(m3•d), while the TNE decreased to 60%, and the NRR decreased to 0.236 kgN/(m3•d) when the COD/N ratio was elevated to 1.0. For the EPS, the amounts of soluble EPS (SEPS) and loosely bound EPS (LB-EPS) were both higher in the CANON stage than in the SNAD stage, while the amount of tightly bound EPS (TB-EPS) in the SNAD stage was significantly higher due to the proliferation of heterotrophic bacteria. The metagenome sequencing technique was used to analyse the microbial community in the SBMBR. The results showed that the addition of COD altered the structure of the bacterial community in the SBMBR. The amounts of Candidatus ‘Anammoxoglobus’ of anaerobic ammonia oxidation bacteria (AAOB) and Nitrosomonas of ammonia oxidizing bacteria (AOB) both decreased significantly, and Nitrospira of nitrite oxidizing bacteria (NOB) was always in the reactor, although the amount changed slightly. A proliferation of denitrifiers related to the genera of Thauera, Dokdonella and Azospira was found in the SBMBR.

scholarly journals Development of Strategies for AOB and NOB Competition Supported by Mathematical Modeling in Terms of Successful Deammonification Implementation for Energy-Efficient WWTPs

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 562
Author(s):  
Mehdi Sharif Shourjeh ◽  
Przemysław Kowal ◽  
Xi Lu ◽  
Li Xie ◽  
Jakub Drewnowski
Keyword(s):  
Mathematical Modeling ◽  
Ammonia Oxidation ◽  
Current Knowledge ◽  
Microbial Interactions ◽  
Process Efficiency ◽  
Ammonia Oxidizing Bacteria ◽  
Operational Conditions ◽  
Novel Technologies ◽  
Anaerobic Ammonia Oxidation ◽  
Nitrite Oxidizing Bacteria

Novel technologies such as partial nitritation (PN) and partial denitritation (PDN) could be combined with the anammox-based process in order to alleviate energy input. The former combination, also noted as deammonification, has been intensively studied in a frame of lab and full-scale wastewater treatment in order to optimize operational costs and process efficiency. For the deammonification process, key functional microbes include ammonia-oxidizing bacteria (AOB) and anaerobic ammonia oxidation bacteria (AnAOB), which coexisting and interact with heterotrophs and nitrite oxidizing bacteria (NOB). The aim of the presented review was to summarize current knowledge about deammonification process principles, related to microbial interactions responsible for the process maintenance under varying operational conditions. Particular attention was paid to the factors influencing the targeted selection of AOB/AnAOB over the NOB and application of the mathematical modeling as a powerful tool enabling accelerated process optimization and characterization. Another reviewed aspect was the potential energetic and resources savings connected with deammonification application in relation to the technologies based on the conventional nitrification/denitrification processes.

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