A direct transfer solution for digital laser printing of CVD graphene

2D Materials ◽  
2021 ◽  
Author(s):  
Symeon Papazoglou ◽  
Dimitrios Kaltsas ◽  
Adamantia Logotheti ◽  
Amaia Pesquera ◽  
Amaia Zurutuza ◽  
...  
2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Daria Antipova ◽  
Leila Eadie ◽  
Ashish Stephen Macaden ◽  
Philip Wilson

Abstract Introduction A number of pre-hospital clinical assessment tools have been developed to triage subjects with acute stroke due to large vessel occlusion (LVO) to a specialised endovascular centre, but their false negative rates remain high leading to inappropriate and costly emergency transfers. Transcranial ultrasonography may represent a valuable pre-hospital tool for selecting patients with LVO who could benefit from rapid transfer to a dedicated centre. Methods Diagnostic accuracy of transcranial ultrasonography in acute stroke was subjected to systematic review. Medline, Embase, PubMed, Scopus, and The Cochrane Library were searched. Published articles reporting diagnostic accuracy of transcranial ultrasonography in comparison to a reference imaging method were selected. Studies reporting estimates of diagnostic accuracy were included in the meta-analysis. Results Twenty-seven published articles were selected for the systematic review. Transcranial Doppler findings, such as absent or diminished blood flow signal in a major cerebral artery and asymmetry index ≥ 21% were shown to be suggestive of LVO. It demonstrated sensitivity ranging from 68 to 100% and specificity of 78–99% for detecting acute steno-occlusive lesions. Area under the receiver operating characteristics curve was 0.91. Transcranial ultrasonography can also detect haemorrhagic foci, however, its application is largely restricted by lesion location. Conclusions Transcranial ultrasonography might potentially be used for the selection of subjects with acute LVO, to help streamline patient care and allow direct transfer to specialised endovascular centres. It can also assist in detecting haemorrhagic lesions in some cases, however, its applicability here is largely restricted. Additional research should optimize the scanning technique. Further work is required to demonstrate whether this diagnostic approach, possibly combined with clinical assessment, could be used at the pre-hospital stage to justify direct transfer to a regional thrombectomy centre in suitable cases.


RSC Advances ◽  
2016 ◽  
Vol 6 (87) ◽  
pp. 83954-83962 ◽  
Author(s):  
Alexander Yulaev ◽  
Guangjun Cheng ◽  
Angela R. Hight Walker ◽  
Ivan V. Vlassiouk ◽  
Alline Myers ◽  
...  

Anthracene was proposed as a new easily evaporable sacrificial layer for a dry CVD graphene transfer.


2014 ◽  
Vol 63 (9) ◽  
pp. 1143-1147 ◽  
Author(s):  
Katherine Woods ◽  
David Beighton ◽  
John L. Klein

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) provides rapid, accurate and cost-effective identification of a range of bacteria and is rapidly changing the face of routine diagnostic microbiology. However, certain groups of bacteria, for example streptococci (in particular viridans or non-haemolytic streptococci), are less reliably identified by this method. We studied the performance of MALDI-TOF MS for identification of the ‘Streptococcus anginosus group’ (SAG) to species level. In total, 116 stored bacteraemia isolates identified by conventional methods as belonging to the SAG were analysed by MALDI-TOF MS. Partial 16S rRNA gene sequencing, supplemented with sialidase activity testing, was performed on all isolates to provide ‘gold standard’ identification against which to compare MALDI-TOF MS performance. Overall, 100 % of isolates were correctly identified to the genus level and 93.1 % to the species level by MALDI-TOF MS. However, only 77.6 % were correctly identified to the genus level and 59.5 % to the species level by a MALDI-TOF MS direct transfer method alone. Use of a rapid in situ extraction method significantly improved identification rates when compared with the direct transfer method (P<0.001). We recommend routine use of this method to reduce the number of time-consuming full extractions required for identification of this group of bacteria by MALDI-TOF MS in the routine diagnostic laboratory. Only 22 % (1/9) of Streptococcus intermedius isolates were reliably identified by MALDI-TOF MS to the species level, even after full extraction. MALDI-TOF MS reliably identifies S. anginosus and Streptococcus constellatus to the species level but does not reliably identify S. intermedius.


1949 ◽  
Vol 27c (4) ◽  
pp. 179-189 ◽  
Author(s):  
A. M. Adams

The superiority of methods involving the use of sporulation media containing acetate, first introduced by Stantial and Elder, over several commonly employed methods is established. A new method for obtaining ascospores from bakers' yeast cultures is recommended involving the direct transfer of vegetative cells from a solid nutrient medium to a solid medium containing acetate. High yields of ascospores are consistently produced after seven days' incubation. This method should lend itself particularly to use in the preparation of ascospores for instructional work, and for genetic research in yeast, and may also find application in yeast taxonomy. The technique recommended is as follows: vegetative yeast cells are multiplied on tomato juice agar or on dextrose nutrient agar, and are then transferred to a solid sporulation medium containing 0.04% dextrose, 0.14% anhydrous sodium acetate, and 2% agar.


2013 ◽  
Author(s):  
Federico Valmorra ◽  
Giacomo Scalari ◽  
Curdin Maissen ◽  
Wangyang Fu ◽  
Christian Schönenberger ◽  
...  

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