Nonsurgical Embryo Recovery as a Feasible Tool for Supporting Embryo Biobanks of Locally Adapted Brazilian Sheep and Goats

The potential of laparoscopic ovum pick-up (LOPU) followed by in vitro embryo production (IVEP) as a tool for accelerated genetic programs in ruminants is reviewed in this article. In sheep and goats, the LOPU-IVEP platform offers the possibility of producing more offspring from elite females, as the procedure is minimally invasive and can be repeated more times and more frequently in the same animals compared with conventional surgical embryo recovery. On average, ~10 and ~14 viable oocytes are recovered by LOPU from sheep and goats, respectively, which results in 3–5 transferable embryos and >50% pregnancy rate after transfer. LOPU-IVEP has also been applied to prepubertal ruminants of 2–6 months of age, including bovine and buffalo calves. In dairy cattle, the technology has gained momentum in the past few years stemming from the development of genetic marker selection that has allowed predicting the production phenotype of dairy females from shortly after birth. In Holstein calves, we obtained an average of ~22 viable oocytes and ~20% transferable blastocyst rate, followed by >50% pregnancy rate after transfer, declaring the platform ready for commercial application. The present and future of this technology are discussed with a focus on improvements and research needed.

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Nonsurgical embryo recovery and transfer in sheep and goats

Theriogenology ◽

10.1016/j.theriogenology.2016.04.025 ◽

2016 ◽

Vol 86 (1) ◽

pp. 144-151 ◽

Cited By ~ 22

Author(s):

Jeferson F. Fonseca ◽

Joanna Maria G. Souza-Fabjan ◽

Maria Emília F. Oliveira ◽

Ceci R. Leite ◽

Paula Maria P. Nascimento-Penido ◽

...

Keyword(s):

Sheep And Goats ◽

Embryo Recovery

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Forage palatability of Broussonetia papyrifera an invasive species in Ghana: Relative preference and palatability by sheep and goats

Journal of Energy and Natural Resource Management ◽

10.26796/jenrm.v2i0.46 ◽

2018 ◽

Vol 2 (2) ◽

pp. 63-70 ◽

Cited By ~ 1

Author(s):

R. Obour

Keyword(s):

Invasive Species ◽

Leucaena Leucocephala ◽

Condensed Tannin ◽

Block Design ◽

Wet Season ◽

Broussonetia Papyrifera ◽

Sheep And Goats ◽

Relative Preference ◽

Exotic Tree ◽

High Level

Broussonetia papyrifera is an exotic tree widely grown for paper production. Due to its prolific regeneration it has invaded forestcanopy gaps and degraded farmlands and has now become an invasive species in Ghana. In enhancing its value for use the plantwas evaluated as potential forage for grazing animals vis-à-vis other two existing forage plants: Ficus exasperata and Leucaenaleucocephala.The study assessed the palatability and preference of Broussonetia papyrifera using sheep and goats for the wet anddry seasons.The species were assessed in indoor pen feeding trials using eight-unit (3×3 m) pens with the cafeteria method.The amount of forage offered was 100g (fresh material) in all instances for each species and for ten minutes. Adesign basedon 3×2×2 factorial in Randomized Complete Block Design (RCBD) was used to test the differences in palatability betweenthe three forage species.Results revealed that palatability was higher (P<0.05) in Leucaena leucocephala compared with Ficusexasperata and Broussonetia papyrifera for sheep and goats across seasons. The trend shown might be the result of the effectsof familiarity with the Leucaena leucocephala since animals tend to select plants that are familiar than newly introduced andunfamiliar plants. The study also revealed high level of condensed tannin (CT) in Broussonetia papyrifera which might haveinterfered with forage intake by the animals.There were no significant differences in palatability of Broussonetia papyrifera forgoat in both dry and wet season interactions and Ficus exasperata for goat in both dry and wet season interactions (P>0.05).Thestudy concluded that Broussonetia papyrifera could be a potential feed for both sheep and goats across seasons.The researchrecommended that livestock farmers should incorporate Broussonetia papyrifera feed into their programmes for both sheep andgoats and should be introduced to animals from infancy so that it may become a familiar feed for them.

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Official identification eartags for sheep and goats. Specification

10.3403/03289391 ◽

2005 ◽

Keyword(s):

Sheep And Goats

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Genetic relationships of domestic sheep and goats in the lower reaches of the Yellow River based on microsatellite analysis

Biodiversity Science ◽

10.3724/sp.j.1003.2008.07050 ◽

2008 ◽

Vol 16 (1) ◽

pp. 53 ◽

Cited By ~ 3

Author(s):

Wang Jianmin ◽

Yue Wenbin

Keyword(s):

Yellow River ◽

Genetic Relationships ◽

Microsatellite Analysis ◽

The Yellow River ◽

Domestic Sheep ◽

Sheep And Goats

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Antimicrobial Resistance and Virulence Characterization of E. Coli Isolated from Subclinical Mastitic Sheep and Goats

Benha Veterinary Medical Journal ◽

10.21608/bvmj.2018.44977 ◽

2018 ◽

Vol 34 (3) ◽

pp. 267-278

Author(s):

Ashraf A. Abd El-Tawab ◽

Mohamed G. Aggour ◽

Fatma I. El- Hofy ◽

Marwa M. Y. El- Mesalami

Keyword(s):

Antimicrobial Resistance ◽

Sheep And Goats ◽

E Coli

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MOLECULAR CHARACTERIZATION OF ESCHERICHIA COLI ISOLATED FROM SHEEP AND GOATS

Journal of Current Veterinary Research ◽

10.21608/jcvr.2018.38789 ◽

2018 ◽

Vol 12 (1) ◽

pp. 7-16

Author(s):

Mohamed Sabry ◽

Ashraf Awad

Keyword(s):

Escherichia Coli ◽

Molecular Characterization ◽

Sheep And Goats

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Molecular Detection of Bacterial Pathogens Directly from the Nasal Swabs and Lung Tissue of Sheep and Goats

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.15.2.6 ◽

2019 ◽

Vol 15 (02) ◽

pp. 22-25

Author(s):

Sunaina Thakur ◽

Subhash Verma ◽

Prasenjit Dhar ◽

Mandeep Sharma

Keyword(s):

Pasteurella Multocida ◽

Direct Detection ◽

Bacterial Species ◽

Economic Losses ◽

Isolation And Identification ◽

Sheep And Goats ◽

Histophilus Somni ◽

Nasal Swabs ◽

Mycoplasma Spp

Respiratory infections of sheep and goats cause heavy morbidity and mortality, leading to huge economic losses. Conventional methods of diagnosis that include isolation and identification of incriminating microbes are time-consuming and fraught with logistic challenges. Direct detection of incriminating microbes using molecular tools is gaining popularity in clinical, microbiological settings. In this study, a total of 50 samples (44 nasal swabs and 6 lung tissues) from sheep and goats were screened for the detection of different bacterial species by in vitro amplification of genus or species-specific genes. Histophilus somni was detected in 2% goat samples, Trueperella pyogenes in 20% goat nasal swabs, whereas 22% goat nasal swab samples were found positive for Mycoplasma spp. None of the samples from sheep was detected positive for H. somni, T. pyogenes, Mycoplasma spp. Similarly, all samples, irrespective, whether from sheep or goats, showed negative results for Pasteurella multocida, Mannheimia haemolytica, and Corynebacterium pseudotuberculosis.

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