Stakeholder Views on Engagement, Trust, Performance, and Risk Considerations About Use of Gene Drive Technology in Agricultural Pest Management

2021 ◽  
Author(s):  
Carol L. Goldsmith ◽  
Ki Eun Kang ◽  
Elizabeth Heitman ◽  
Zach N. Adelman ◽  
Leah W. Buchman ◽  
...  
2018 ◽  
Vol 115 (24) ◽  
pp. 6189-6194 ◽  
Author(s):  
Mohammad KaramiNejadRanjbar ◽  
Kolja N. Eckermann ◽  
Hassan M. M. Ahmed ◽  
Héctor M. Sánchez C. ◽  
Stefan Dippel ◽  
...  

The use of a site-specific homing-based gene drive for insect pest control has long been discussed, but the easy design of such systems has become possible only with the recent establishment of CRISPR/Cas9 technology. In this respect, novel targets for insect pest management are provided by new discoveries regarding sex determination. Here, we present a model for a suppression gene drive designed to cause an all-male population collapse in an agricultural pest insect. To evaluate the molecular details of such a sex conversion-based suppression gene drive experimentally, we implemented this strategy in Drosophila melanogaster to serve as a safe model organism. We generated a Cas9-based homing gene-drive element targeting the transformer gene and showed its high efficiency for sex conversion from females to males. However, nonhomologous end joining increased the rate of mutagenesis at the target site, which resulted in the emergence of drive-resistant alleles and therefore curbed the gene drive. This confirms previous studies that simple homing CRISPR/Cas9 gene-drive designs will be ineffective. Nevertheless, by performing population dynamics simulations using the parameters we obtained in D. melanogaster and by adjusting the model for the agricultural pest Ceratitis capitata, we were able to identify adequate modifications that could be successfully applied for the management of wild Mediterranean fruit fly populations using our proposed sex conversion-based suppression gene-drive strategy.


Insects ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 484
Author(s):  
Syed Arif Hussain Rizvi ◽  
Justin George ◽  
Gadi V. P. Reddy ◽  
Xinnian Zeng ◽  
Angel Guerrero

Since the first identification of the silkworm moth sex pheromone in 1959, significant research has been reported on identifying and unravelling the sex pheromone mechanisms of hundreds of insect species. In the past two decades, the number of research studies on new insect pheromones, pheromone biosynthesis, mode of action, peripheral olfactory and neural mechanisms, and their practical applications in Integrated Pest Management has increased dramatically. An interdisciplinary approach that uses the advances and new techniques in analytical chemistry, chemical ecology, neurophysiology, genetics, and evolutionary and molecular biology has helped us to better understand the pheromone perception mechanisms and its practical application in agricultural pest management. In this review, we present the most recent developments in pheromone research and its application in the past two decades.


1982 ◽  
Vol 30 (4) ◽  
pp. 601-618 ◽  
Author(s):  
Richard M. Feldman ◽  
Guy L. Curry

Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1156 ◽  
Author(s):  
Jessica P. Yactayo-Chang ◽  
Hoang V. Tang ◽  
Jorrel Mendoza ◽  
Shawn A. Christensen ◽  
Anna K. Block

Insect pests cause significant global agricultural damage and lead to major financial and environmental costs. Crops contain intrinsic defenses to protect themselves from such pests, including a wide array of specialized secondary metabolite-based defense chemicals. These chemicals can be induced upon attack (phytoalexins) or are constitutive (phytoanticipins), and can have a direct impact on the pests or be used indirectly to attract their natural enemies. They form part of a global arms race between the crops and their insect pests, with the insects developing methods of suppression, avoidance, detoxification, or even capture of their hosts defensive chemicals. Harnessing and optimizing the chemical defense capabilities of crops has the potential to aid in the continuing struggle to enhance or improve agricultural pest management. Such strategies include breeding for the restoration of defense chemicals from ancestral varieties, or cross-species transfer of defense metabolite production.


2017 ◽  
Vol 4 (6) ◽  
pp. 170210 ◽  
Author(s):  
P. Dilip Venugopal ◽  
Galen P. Dively

Increased temperature anomaly during the twenty-first century coincides with the proliferation of transgenic crops containing the bacterium Bacillus thuringiensis (Berliner) (Bt) to express insecticidal Cry proteins. Increasing temperatures profoundly affect insect life histories and agricultural pest management. However, the implications of climate change on Bt crop–pest interactions and insect resistance to Bt crops remains unexamined. We analysed the relationship of temperature anomaly and Bt adoption with field-evolved resistance to Cry1Ab Bt sweet corn in a major pest, Helicoverpa zea (Boddie). Increased Bt adoption during 1996–2016 suppressed H. zea populations, but increased temperature anomaly buffers population reduction. Temperature anomaly and its interaction with elevated selection pressure from high Bt acreage probably accelerated the Bt-resistance development. Helicoverpa zea damage to corn ears, kernel area consumed, mean instars and proportion of late instars in Bt varieties increased with Bt adoption and temperature anomaly, through additive or interactive effects. Risk of Bt-resistant H. zea spreading is high given extensive Bt adoption, and the expected increase in overwintering and migration. Our study highlights the challenges posed by climate change for Bt biotechnology-based agricultural pest management, and the need to incorporate evolutionary processes affected by climate change into Bt-resistance management programmes.


2012 ◽  
Vol 12 (53) ◽  
pp. 6582-6592
Author(s):  
SC Eze ◽  
◽  
BC Echezona

Agricultural pest management control strategies are primarily concerned with food security and safety. Popular pest control methods include application of synthetic pesticides, biopesticides (plant extracts), non-chemical pest management and integrated pest management (IPM). The resistance of some of the pests to the chemical pesticides, coupled with potential health hazards on the environment gave birth to a search for botanicals as alternatives to synthetic pesticides. Botanicals as biopesticides were, though effective but their shelf lives and specific actions on the target organisms have not been determined. Non-chemical pest control methods involve common cultural practices which include crop rotation, tillage, and varying time of planting or harvesting, trap cropping which appear to be the best in terms of food safety and quality but the ability of this approach to reduce pest population may be minimal. Because no single pest control method can guarantee food security and safety, integrated pest management (IPM) approach appears to hold promise. The IPM is an ecologically based approach that combines all the available pest control methods to manage pest damage by the most economical means, with the fewest possible hazards to life, property and environment. However, this review shows that the impact of integrated pest management in the rural farm communities is low. In an era of growing consumer awareness and sophistication, food quality is being emphasized. Food safety means that the agro-products should be free from pesticide residues:- therefore, aspects of farm management such as sources of seeds and seedlings, pests and weed elimination, pesticide application dates, dates and amount of fertilization, harvesting or post harvest treatments and basic information regarding the individual farmer or marketing agents activities should be certified before consuming agricultural products. Federal governments especially in developing countries are advised to mount regulating Agencies that will be responsible for a number of activities that contribute to food security and safety, water quality and pesticide applicator training as practiced in the United States of America, India and Indonesia. The agencies will ensure that the public is protected from potential health risks posed by pesticide treated foods.


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