Engineering NK-92 Cell by Upregulating CXCR2 and IL-2 Via CRISPR-Cas9 Improves Its Antitumor Effects as Cellular Immunotherapy for Human Colon Cancer

Colon cancer is one of the deadliest tumors in the world, and with high metastasis rate and mortality, effective drugs for its treatment are still in need. Auranofin (AF) is a gold complex that has been attested by FDA for treating human rheumatism, and researchers have found that AF acts as a great antitumor drug in recent years. ICG-001 is a small molecule inhibitor of Wnt/β-catenin pathway. In the present study, we aimed to explore the synergistic antitumor effects and the underlying mechanisms of AF and ICG-001 combination therapy on human colon cancer. The results showed that AF and ICG-001 synergistically depressed the growth and invasion of human colon cancer cells by inhibiting the phosphorylation of Signal Transducer and Activator of Transcription 3 (STAT3) and its downstream mediator B-cell lymphoma-2-like 1 (Bcl-xL) and inducing caspase-3-dependent apoptosis. Moreover, AF combined with ICG-001 synergistically inhibited the growth of colon cancer in subcutaneous xenograft mice models and restrained metastasis in lung metastasis mice models. In conclusion, our results demonstrated that combination of AF and ICG-001 suppressed the proliferation and metastasis of colon cancer by inhibiting STAT3 phosphorylation. Therefore, this combination therapy may possess potential therapeutic properties for human colon cancer.

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Antitumor effects of emodin on LS1034 human colon cancer cells in vitro and in vivo: Roles of apoptotic cell death and LS1034 tumor xenografts model

Food and Chemical Toxicology ◽

10.1016/j.fct.2012.01.033 ◽

2012 ◽

Vol 50 (5) ◽

pp. 1271-1278 ◽

Cited By ~ 74

Author(s):

Yi-Shih Ma ◽

Shu-Wen Weng ◽

Meng-Wei Lin ◽

Chi-Cheng Lu ◽

Jo-Hua Chiang ◽

...

Keyword(s):

Colon Cancer ◽

Apoptotic Cell ◽

Human Colon ◽

Colon Cancer Cells ◽

Antitumor Effects ◽

Human Colon Cancer ◽

Tumor Xenografts ◽

Human Colon Cancer Cells

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Antitumor effects of 5-fluorouracil on human colon cancer cell lines: antagonism by levamisole

Journal of Surgical Research ◽

10.1016/s0022-4804(03)00053-2 ◽

2003 ◽

Vol 111 (1) ◽

pp. 63-69 ◽

Cited By ~ 12

Author(s):

Eric A Wiebke ◽

Neil A Grieshop ◽

Patrick J Loehrer ◽

George J Eckert ◽

Richard A Sidner

Keyword(s):

Colon Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Human Colon ◽

Cancer Cell Lines ◽

Colon Cancer Cell ◽

Human Colon Cancer Cell ◽

Antitumor Effects ◽

Human Colon Cancer ◽

Colon Cancer Cell Lines

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Antitumor effects of calgranulin B internalized in human colon cancer cells

Oncotarget ◽

10.18632/oncotarget.7783 ◽

2016 ◽

Vol 7 (15) ◽

pp. 20368-20380 ◽

Cited By ~ 4

Author(s):

Kun Kim ◽

Kyung-Hee Kim ◽

Kangsan Roh ◽

Byong Chul Yoo ◽

Ja-Lok Ku ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Human Colon ◽

Colon Cancer Cells ◽

Antitumor Effects ◽

Human Colon Cancer ◽

Calgranulin B ◽

Human Colon Cancer Cells

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Ganoderma lucidum Polysaccharide Enzymatic Hydrolysate Suppresses the Growth of Human Colon Cancer Cells via Inducing Apoptosis

Cell Transplantation ◽

10.1177/0963689720931435 ◽

2020 ◽

Vol 29 ◽

pp. 096368972093143

Author(s):

Jing hui Bai ◽

Jian Xu ◽

Jian Zhao ◽

Rui Zhang

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Ganoderma Lucidum ◽

B Cell Lymphoma ◽

Human Colon ◽

Colon Cancer Cells ◽

Antitumor Effects ◽

Human Colon Cancer ◽

Hct 116 Cells ◽

Human Colon Cancer Cells

Ganoderma lucidum is a popular traditional Chinese medicine used in China to improve health. Previous researches have revealed that the polysaccharide from G. lucidum could exert diversity activities, including immunomodulation, antioxidant, and antitumor effects. However, the effect of enzymatically hydrolyzed G. lucidum polysaccharide (EGLP) in colorectal cancer (CRC) progression remains unknown. The present research aimed to investigate the antitumor mechanism of EGLP in human colon cancer cells. For this purpose, the cytotoxic effects of EGLP were measured by the (3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) method. The apoptosis was evoked upon EGLP treatment, which was assayed using flow cytometry. The results indicated that EGLP may induce apoptosis in human colon cancer cell (HCT-116) cells via the upregulation of BCL-2 associated X protein (Bax), phospho-extracellular regulated protein kinases (P-ERK), and cleaved caspase-3 expression and downregulation of B-cell lymphoma-2 (Bcl-2), phospho-serine/threonine kinase 1 (p-Akt1), and cyclo-oxygen-ase (COX-2) expression. The obtained findings indicated EGLP as a new therapeutic agent in fighting CRC.

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Antiproliferative activities of Artemisia herba-alba ethanolic extract in human colon cancer cell line (HCT116)

Alternative Medicine Studies ◽

10.4081/ams.2011.e14 ◽

2011 ◽

Vol 1 (1) ◽

pp. 14 ◽

Cited By ~ 6

Author(s):

Giulio Lupidi ◽

Massimo Bramucci ◽

Luana Quassinti ◽

Enzo Fornari ◽

Luca Avenali ◽

...

Keyword(s):

Cell Cycle ◽

Colon Cancer ◽

Dna Fragmentation ◽

Cancer Cell Line ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Human Colon ◽

Antitumor Effects ◽

Human Colon Cancer ◽

Hct116 Cells

Artemisia herba-alba (AHE) is a plant commonly used in traditional medicine for the treatment of various ailments. Here, we investigated the antioxidant and antitumor activity of the aqueous and ethanol extracts of AHE in human colon cancer HCT116 cells. The antioxidant activity was measured by DCFH assay, while antitumor effects were assessed by cell viability assays, cell cycle progression by flow cytometry, and DNA fragmentation analysis in addition to investigating the expression of key cell cycle and apoptotic proteins. While the aqueous extract had no antineoplastic effects, the ethanol extract significantly decreased HCT116 viability (IC50 of 51mg/mL at 24 h) and inhibited the production of reactive oxygen species (ROS). Treatment of HCT116 cells with the ethanol extract also caused dramatic increase in the PreG1 population with concomitant decrease in cycling cells, provoked DNA fragmentation, significant increase in the expression levels of p53 and Bax proteins and activated pro-apoptotic caspase-3. The results obtained suggest that the ethanol extract of AHE could be used as an easily accessible source of natural antioxidants and as potential phytochemicals against colon cancer.

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