Inhibition of Acetylcholinesterase Activity and Fe2+-Induced Lipid Peroxidation in Rat Brain In Vitro by Some Citrus Fruit Juices

The antioxidative activity of ferrocenes bearing either 2,6-di-tert-butylphenol or phenyl groups has been compared using DPPH (1,1-diphenyl-2-picrylhydrazyl) test and in the study of the in vitro impact on lipid peroxidation in rat brain homogenate and on some characteristics of rat liver mitochondria. The results of DPPH test at20∘C show that the activity depends strongly upon the presence of phenolic group but is improved by the influence of ferrocenyl fragment. The activity of N-(3,5-di-tert-butyl-4-hydroxyphenyl)iminomethylferrocene (1), for instance, was 88.4%, which was higher than the activity of a known antioxidant 2,6-di-tert-butyl-4-methylphenol (BHT) (48.5%), whereas the activity of N-phenyl-iminomethylferrocene2was almost negligible−2.9%. The data obtained demonstrate that the compounds with 2,6-di-tert-butylphenol moiety are significantly more active than the corresponding phenyl analogues in the in vitro study of lipid peroxidation in rat brain homogenate. Ferrocene1performs a promising behavior as an antioxidant and inhibits the calcium-dependent swelling of mitochondria. These results allow us to propose the potential cytoprotective (neuroprotective) effect of ditopic compounds containing antioxidant 2,6-di-tert-butylphenol group and redox active ferrocene fragment.

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Suckling Rat Brain Regional Distribution of Acetylcholinesterase Activity in Galactosaemia In Vitro

Metabolic Brain Disease ◽

10.1007/s11011-005-7210-3 ◽

2005 ◽

Vol 20 (3) ◽

pp. 227-236 ◽

Cited By ~ 8

Author(s):

Kyriakoula Marinou ◽

Stylianos Tsakiris ◽

Christi Tsopanakis ◽

Kleopatra H. Schulpis ◽

Panagiotis Behrakis

Keyword(s):

Rat Brain ◽

Regional Distribution ◽

Acetylcholinesterase Activity

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In Vitro Evaluation of Free Radical Scavenging, Fe2+ and SNP-Induced Lipid Peroxidation (Rat Brain) Activities of Methanolic Extracts from Three (3) Northern Nigerian Plants Leaf

Journal of Tropical Life Science ◽

10.11594/jtls.09.01.10 ◽

2019 ◽

Vol 9 (1) ◽

pp. 71-78

Author(s):

Augustina Akinsami ◽

Omolara Johnson ◽

Ishaya Longdet ◽

John Aguiyi

Keyword(s):

Lipid Peroxidation ◽

Free Radical ◽

Rat Brain ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

In Vitro Evaluation ◽

Methanolic Extracts

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Antioxidative Potential of Ocimum gratissimum and Ocimum canum Leaf Polyphenols and Protective Effects on Some Pro-Oxidants Induced Lipid Peroxidation in Rat Brain: An in vitro Study

American Journal of Food Technology ◽

10.3923/ajft.2008.325.334 ◽

2008 ◽

Vol 3 (5) ◽

pp. 325-334 ◽

Cited By ~ 19

Author(s):

Ganiyu Oboh

Keyword(s):

Lipid Peroxidation ◽

Rat Brain ◽

In Vitro Study ◽

Vitro Study ◽

Protective Effects ◽

Ocimum Gratissimum

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Local salt substitutes “Obu-otoyo” activate acetylcholinesterase and butyrylcholinesterase and induce lipid peroxidation in rat brain

Interdisciplinary Toxicology ◽

10.1515/intox-2015-0021 ◽

2015 ◽

Vol 8 (3) ◽

pp. 139-145 ◽

Cited By ~ 1

Author(s):

Ayodele J. Akinyemi ◽

Ganiyu Oboh ◽

Adedayo O. Ademiluyi

Keyword(s):

Heavy Metals ◽

Lipid Peroxidation ◽

Rat Brain ◽

Palm Kernel ◽

Dependent Manner ◽

Toxic Heavy Metals ◽

Induce Lipid Peroxidation ◽

Mda Content

Abstract Evidence has shown that ingestion of heavy metals can lead to neurodegenerative diseases. This study aimed to investigate the neurotoxic potential of salt substitutes (Obu-Otoyo); salt A (made by burning palm kernel shaft then soaked in water overnight and the extract from the resulting residue is used as the salt substitute) and salt B (an unrefined salt mined from a local site at Ilobu town, Osun-State, Nigeria) by assessing their effect on some key enzymes linked with neurodegenerative disease [acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities] as well as on malondialdehyde (MDA) content of the rat brain. Salt substitutes were fed to normal rats as dietary inclusion at doses of 0.5 and 1.0% for 30 days. Thereafter, the effect of the salt substitutes on AChE and BChE activities as well as on MDA level in the rat brain was determined. The results revealed that the salt substitutes caused a significant (p<0.05) increase in both AChE and BChE activity and also induced lipid peroxidation in the brain of rats in vivo as well as under in vitro condition in a dose-dependent manner. The effect of the salt substitutes on AChE and BChE activities could be attributed to the presence of some toxic heavy metals. Therefore, the ability of the salt substitutes to induce lipid peroxidation and activate AChE and BChE activities could provide some possible mechanism for their neurotoxic effect.

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