Kainate-induced lipid peroxidation in rat brain in vitro is markedly reduced by the endogenous antioxidant melatonin

1995 ◽  
Vol 31 ◽  
pp. 295
Author(s):  
Daniela Melchiorri ◽  
Russel J. Reiter ◽  
Ewa Sewerynek ◽  
Li Dun Chen ◽  
Giuseppe Nisticò
Keyword(s):  
Lipid Peroxidation ◽  
Rat Brain ◽  
Endogenous Antioxidant

scholarly journals Neuroprotective Effects of a Variety of Pomegranate Juice Extracts against MPTP-Induced Cytotoxicity and Oxidative Stress in Human Primary Neurons

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Nady Braidy ◽  
Subash Selvaraju ◽  
Musthafa Mohamed Essa ◽  
Ragini Vaishnav ◽  
Samir Al-Adawi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Pomegranate Juice ◽  
Neuroprotective Effects ◽  
Age Related ◽  
Therapeutic Value ◽  
Human Neurons ◽  
Environmental Toxin ◽  
Endogenous Antioxidant ◽  
Therapeutic Compounds

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is an environmental toxin which selectively induces oxidative damage and mitochondrial and proteasomal dysfunctions to dopaminergic neurons in the substantia nigra leading to Parkinsonian syndrome in animal models and humans. MPTP is one of the most widely usedin vitromodels to investigate the pathophysiology of Parkinson's disease (PD) and, screen for novel therapeutic compounds that can slow down or ameliorate this progressive degenerative disease. We investigated the therapeutic effect of pomegranate juice extracts (PJE), Helow, Malasi, Qusum, and Hamadh against MPTP-induced neurotoxicity in primary human neurons by examining extracellular LDH activity, intracellular NAD+and ATP levels, and endogenous antioxidant levels including lipid peroxidation products, catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities, and reduced glutathione (GSH) levels. MPTP induced a reduction in SOD and GPx activities and intracellular NAD+, ATP, and GSH levels parallel to an increase in extracellular LDH and CAT activities, although lipid peroxidation was not altered. We report that helow and malasi can ameliorate MPTP-induced neurotoxicity by attenuating the observed changes in redox function to a greater extent than qusum and hamedh. Selected PJE varieties may exhibit properties which may be of therapeutic value to slow down age-related degeneration and neurodegeneration in particular.

scholarly journals Antioxidative Activity of Ferrocenes Bearing 2,6-Di-Tert-Butylphenol Moieties

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
E. R. Milaeva ◽  
S. I. Filimonova ◽  
N. N. Meleshonkova ◽  
L. G. Dubova ◽  
E. F. Shevtsova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Rat Brain ◽  
Antioxidative Activity ◽  
Neuroprotective Effect ◽  
In Vitro Study ◽  
Brain Homogenate ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Calcium Dependent

The antioxidative activity of ferrocenes bearing either 2,6-di-tert-butylphenol or phenyl groups has been compared using DPPH (1,1-diphenyl-2-picrylhydrazyl) test and in the study of the in vitro impact on lipid peroxidation in rat brain homogenate and on some characteristics of rat liver mitochondria. The results of DPPH test at20∘C show that the activity depends strongly upon the presence of phenolic group but is improved by the influence of ferrocenyl fragment. The activity of N-(3,5-di-tert-butyl-4-hydroxyphenyl)iminomethylferrocene (1), for instance, was 88.4%, which was higher than the activity of a known antioxidant 2,6-di-tert-butyl-4-methylphenol (BHT) (48.5%), whereas the activity of N-phenyl-iminomethylferrocene2was almost negligible−2.9%. The data obtained demonstrate that the compounds with 2,6-di-tert-butylphenol moiety are significantly more active than the corresponding phenyl analogues in the in vitro study of lipid peroxidation in rat brain homogenate. Ferrocene1performs a promising behavior as an antioxidant and inhibits the calcium-dependent swelling of mitochondria. These results allow us to propose the potential cytoprotective (neuroprotective) effect of ditopic compounds containing antioxidant 2,6-di-tert-butylphenol group and redox active ferrocene fragment.

scholarly journals Local salt substitutes “Obu-otoyo” activate acetylcholinesterase and butyrylcholinesterase and induce lipid peroxidation in rat brain

2015 ◽  
Vol 8 (3) ◽  
pp. 139-145 ◽  
Author(s):  
Ayodele J. Akinyemi ◽  
Ganiyu Oboh ◽  
Adedayo O. Ademiluyi
Keyword(s):  
Heavy Metals ◽  
Lipid Peroxidation ◽  
Rat Brain ◽  
Palm Kernel ◽  
Dependent Manner ◽  
Toxic Heavy Metals ◽  
Induce Lipid Peroxidation ◽  
Mda Content

Abstract Evidence has shown that ingestion of heavy metals can lead to neurodegenerative diseases. This study aimed to investigate the neurotoxic potential of salt substitutes (Obu-Otoyo); salt A (made by burning palm kernel shaft then soaked in water overnight and the extract from the resulting residue is used as the salt substitute) and salt B (an unrefined salt mined from a local site at Ilobu town, Osun-State, Nigeria) by assessing their effect on some key enzymes linked with neurodegenerative disease [acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities] as well as on malondialdehyde (MDA) content of the rat brain. Salt substitutes were fed to normal rats as dietary inclusion at doses of 0.5 and 1.0% for 30 days. Thereafter, the effect of the salt substitutes on AChE and BChE activities as well as on MDA level in the rat brain was determined. The results revealed that the salt substitutes caused a significant (p<0.05) increase in both AChE and BChE activity and also induced lipid peroxidation in the brain of rats in vivo as well as under in vitro condition in a dose-dependent manner. The effect of the salt substitutes on AChE and BChE activities could be attributed to the presence of some toxic heavy metals. Therefore, the ability of the salt substitutes to induce lipid peroxidation and activate AChE and BChE activities could provide some possible mechanism for their neurotoxic effect.

scholarly journals Pergolide scavenges both hydroxyl and nitric oxide free radicals in vitro and inhibits lipid peroxidation in different regions of the rat brain

1998 ◽  
Vol 790 (1-2) ◽  
pp. 202-208 ◽  
Author(s):  
Marvin Gómez-Vargas ◽  
Sakiko Nishibayashi-Asanuma ◽  
Masato Asanuma ◽  
Yoichi Kondo ◽  
Emi Iwata ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Free Radicals ◽  
Rat Brain
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