Evaluation In Vitro of Toxicity of Hydroalcoholic Extract of Leaves and Roots from Yacon (Smallanthus sonchifolius)

2020 ◽  
Author(s):  
Patrícia Martinez-Oliveira ◽  
Luísa Zuravski ◽  
Micaela Federizzi de Oliveira ◽  
Deise Jaqueline Ströher ◽  
Ritiéle Pinto Coelho ◽  
...  
Keyword(s):  
Hydroalcoholic Extract ◽  
Smallanthus Sonchifolius ◽  
Leaves And Roots

In vitro evaluation of yacon (Smallanthus sonchifolius) tuber flour prebiotic potential

2015 ◽  
Vol 95 ◽  
pp. 96-105 ◽  
Author(s):  
Sérgio Sousa ◽  
Jorge Pinto ◽  
Claúdia Pereira ◽  
F. Xavier Malcata ◽  
M.T. Bertoldo Pacheco ◽  
...  
Keyword(s):  
In Vitro Evaluation ◽  
Smallanthus Sonchifolius

scholarly journals Human Nails Permeation of an Antifungal Candidate Hydroalcoholic Extract from the Plant Sapindus saponaria L. Rich in Saponins

Molecules ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 236
Author(s):  
Vanessa Mendes ◽  
Flávia Franco Veiga ◽  
Lidiane Vizioli de Castro-Hoshino ◽  
Francielle Sato ◽  
Mauro Luciano Baesso ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Attenuated Total Reflection ◽  
Ionization Mass ◽  
Healthy Human ◽  
Hydroalcoholic Extract ◽  
Sapindus Saponaria ◽  
Infrared Spectrometer ◽  
Low Cytotoxicity ◽  
Human Nails

We evaluated a hydroalcoholic extract of Sapindus saponaria L. pericarps (ETHOSS), as a candidate to a topical antifungal medicine for onychomycosis. ETHOSS was produced by extracting the crushed fruits in ethanol. The saponin contents were identified and characterized by electrospray ionization mass spectrometry. We measured the in vitro antifungal activity against three dermatophyte fungi, isolated from onychomycosis: Trichophyton rubrum, T. mentagrophytes, and T. interdigitale, using broth microdilution tests. The minimum fungicide concentration of ETHOSS ranged from 195.31 to 781.25 μg/mL. The cytotoxicity of the crude extract was tested on the HeLa cell line, and its ability to permeate into healthy human nails by photoacoustic spectroscopy and Fourier transformation infrared spectrometer (FTIR) spectroscopy by attenuated total reflection. Besides its strong antifungal activity, ETHOSS showed low cytotoxicity in human cells. It was able to permeate and reach the full thickness of the nail in one hour, without the aid of facilitating vehicles, and remained there for at least 24 h. These results suggest that ETHOSS has great potential for treating onychomycosis.

Phytochemical analysis of olive flowers' hydroalcoholic extract and in vitro evaluation of tyrosinase, elastase and collagenase inhibition activity

Fitoterapia ◽  
2020 ◽  
Vol 143 ◽  
pp. 104602 ◽  
Author(s):  
Apostolis Angelis ◽  
Panagiotis Mavros ◽  
Panagiota Efstathia Nikolaou ◽  
Sofia Mitakou ◽  
Maria Halabalaki ◽  
...  
Keyword(s):  
Phytochemical Analysis ◽  
Inhibition Activity ◽  
Hydroalcoholic Extract ◽  
In Vitro Evaluation ◽  
Collagenase Inhibition

scholarly journals In-vitro evaluation of Nigella sativa and Punica granatum effect on protoscolices of hydatid cysts

2019 ◽  
Vol 28 (2) ◽  
pp. 210-214 ◽  
Author(s):  
Nasr Moawad El-Bahy ◽  
Amer Ragheb Abdelaziz ◽  
Reda Elbastawisy Khalafalla
Keyword(s):  
Anaphylactic Shock ◽  
Treatment Approach ◽  
Nigella Sativa ◽  
Punica Granatum ◽  
Hydatid Cysts ◽  
Hydroalcoholic Extract ◽  
Hydatid Fluid ◽  
Viability Assay ◽  
Peel Extract

Abstract Cystic echinococcosis (CE) are commonly found in the liver and lungs of affected hosts. The treatment approach is usually surgical, or giving drugs in conjunction before surgery to kill protoscolices, to avoid anaphylactic shock from leakage of hydatid fluid into the peritoneum and to decrease opportunities for recurrences. The present study was to evaluate the in vitro scolicidal efficacy of hydroalcoholic extract of Punica granatum peel and Nigella sativa, on the protoscolices of CE that collected from the lungs of infected camels. Different concentrations of extracts with different exposure times were used and a viability assay was applied to measure the scolicidal effect. N. sativa showed its highest scolicidal efficacy at 100 mg/mL and 10 mg/mL concentrations after 30 and 60 min. P. granatum peel extract showed its maximum scolicidal efficacy at 100 mg/mL concentration after 120 min. All experiments of the current study revealed that the extracts of both N. sativa and P. granatum had a scolicidal effects on the protoscolices of camel hydatid cysts. It could be concluded that N. sativa extract is more potent than P. granatum peel extract regarding scolicidal effect, but the efficacies of both extracts were of moderate significant correlation to exposure time and concentrations.

scholarly journals ASSESMENT OF ANTI-INFLAMMATORY ACTIVITY AND CYTOTOXICITY OF FREEZE DRIED HYDROALCOHOLIC EXTRACT OF Bidens andicola ON ISOLATED NEUTROPHILS

2017 ◽  
Vol 10 (6) ◽  
pp. 160
Author(s):  
Vinueza D ◽  
LÓpez E ◽  
Acosta K ◽  
Abdo S
Keyword(s):  
Freeze Drying ◽  
Water Soluble ◽  
Inflammatory Activity ◽  
Hydroalcoholic Extract ◽  
Cytotoxicity Assays ◽  
Aerial Parts ◽  
Controlled Conditions ◽  
Freeze Dried ◽  
Anti Inflammatory

Objective: The aim of this study was to evaluate anti-inflammatory activity and cytotoxicity in vitro of hydroalcoholic extract of Bidens andicola.Methods: B. andicola hydroalcoholic extract was obtained from aerial parts of B. andicola, following a standardized methodology. Briefly, aerial parts of B. andicola were extracted with ethanol 70% v/v and defatted with n-hexane, hydroalcoholic fraction was concentrated under controlled conditions in a rotary evaporator, and finally the residue was freeze-drying to obtain the hydroalcoholic extract of B. andicola. Anti-inflammatory activity and cytotoxicity assays were carried out using in vitro isolated neutrophils model using stable water-soluble tetrazolium salts.Results and Conclusions: The in vitro anti-inflammatory assay on isolated neutrophils demonstrated that the hydroalcoholic extract showed antiinflammatoryactivity compared to aspirin, with inflammatory inhibition percent values of 80.138±0.729 to hydroalcoholic extract of B. andicola and 82.117±0.762 to aspirin, each tested in five replicates at the concentration of 200 ppm of hydroalcoholic extract or reference. 

scholarly journals STUDY OF IN VITRO ANTI-LITHIATIC EFFECT OF IPOMOEA BATATAS (L) LEAVES AND TUBEROUS ROOTS

2018 ◽  
Vol 11 (2) ◽  
pp. 427 ◽  
Author(s):  
Sathish R ◽  
Jeyabalan G
Keyword(s):  
Calcium Phosphate ◽  
Calcium Oxalate ◽  
Ipomoea Batatas ◽  
Colorimetric Method ◽  
Dependent Manner ◽  
Calcium Oxalate Crystals ◽  
Tuberous Roots ◽  
Oxalate Crystals ◽  
Leaves And Roots

 Objective: The present study was to demonstrate the in vitro anti-lithiatic effect of Ipomoea batatas (Convolvulaceae) leaves and tuberous roots.Methods: The obtained ethanolic extract of I. batatas leaves and tuberous roots (EIBL and EIBR) and aqueous extract of I. batatas leaves and roots (AIBL and AIBR) were used for this in vitro study. The dissolution method of calcium oxalate by titrimetry method and calcium phosphate by colorimetric method was studied. Nucleation and aggregation of calcium oxalate crystals were determined by a spectrophotometric assay.Results: In the estimation of calcium oxalate by titrimetry method, the I. batatas leaves and roots have very significant (p<0.01) capability to dissolve calcium oxalate. Percentage dissolution of calcium oxalate crystals was found to be 37.53%, 22.74%, 39.74%, and 24.28% for EIBL, AIBL, EIBR, and AIBR, respectively. In the estimation of calcium phosphate by colorimetric method, the percentage dissolution of calcium phosphate crystals by EIBL, AIBL, EIBR, and AIBR was found to be 67.15%, 43.17%, 76.74%, and 47.96%, respectively. The I. batatas leaves and roots were significantly (p<0.01) dissolved calcium phosphate also. The results were clearly shown that I. batatas extracts significantly (p<0.01) inhibited both nucleation and aggregation of calcium oxalate crystals by concentration-dependent manner. The maximum percent inhibition of calcium oxalate nucleation by EIBL, AIBL, EIBR, and AIBR was found to be 59.09%, 50.0%, 84.09%, and 47.73%, respectively, at 1000 μg/ml. The EIBL, AIBL, EIBR, and AIBR were inhibited calcium oxalate aggregation by 63.46%, 36.54%, 84.61%, and 42.3%, respectively, at 1000 μg/ml.Conclusions: The results clearly indicate that I. batatas leaves and tuberous roots were found to express in vitro anti-lithiatic potential.

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