scholarly journals ASSESMENT OF ANTI-INFLAMMATORY ACTIVITY AND CYTOTOXICITY OF FREEZE DRIED HYDROALCOHOLIC EXTRACT OF Bidens andicola ON ISOLATED NEUTROPHILS

2017 ◽  
Vol 10 (6) ◽  
pp. 160
Author(s):  
Vinueza D ◽  
LÓpez E ◽  
Acosta K ◽  
Abdo S
Keyword(s):  
Freeze Drying ◽  
Water Soluble ◽  
Inflammatory Activity ◽  
Hydroalcoholic Extract ◽  
Cytotoxicity Assays ◽  
Aerial Parts ◽  
Controlled Conditions ◽  
Freeze Dried ◽  
Anti Inflammatory

Objective: The aim of this study was to evaluate anti-inflammatory activity and cytotoxicity in vitro of hydroalcoholic extract of Bidens andicola.Methods: B. andicola hydroalcoholic extract was obtained from aerial parts of B. andicola, following a standardized methodology. Briefly, aerial parts of B. andicola were extracted with ethanol 70% v/v and defatted with n-hexane, hydroalcoholic fraction was concentrated under controlled conditions in a rotary evaporator, and finally the residue was freeze-drying to obtain the hydroalcoholic extract of B. andicola. Anti-inflammatory activity and cytotoxicity assays were carried out using in vitro isolated neutrophils model using stable water-soluble tetrazolium salts.Results and Conclusions: The in vitro anti-inflammatory assay on isolated neutrophils demonstrated that the hydroalcoholic extract showed antiinflammatoryactivity compared to aspirin, with inflammatory inhibition percent values of 80.138±0.729 to hydroalcoholic extract of B. andicola and 82.117±0.762 to aspirin, each tested in five replicates at the concentration of 200 ppm of hydroalcoholic extract or reference. 

scholarly journals Anti-Inflammatory Activity and Quality Control of Erysimum cheiri (L.) Crantz

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Ghazaleh Mosleh ◽  
Amir Azadi ◽  
Sedigheh Khademian ◽  
Reza Heidari ◽  
Abdolali Mohagheghzadeh
Keyword(s):  
Quality Control ◽  
Diclofenac Sodium ◽  
Water Soluble ◽  
Inflammatory Activity ◽  
Drug Candidate ◽  
Aerial Parts ◽  
Stretching Vibration ◽  
Ft Ir ◽  
Anti Inflammatory

Wallflower (Erysimum cheiri) is employed as a popular herbal drug in traditional Persian medicine. Topical formulations including cerates, lotions, sitz baths, and poultices for inflammatory disorders such as arthritis, anal fissure, endometriosis, and mastitis are known. However, there is no monograph in current pharmacopoeia for the wallflower drug. The present study is aimed to screen in vitro anti-inflammatory activity of wallflower and perform quality control and characterization tests for different organs of the herb. In this regard, albumin denaturation activity, macroscopic and microscopic, phytochemical, HPTLC, and FT-IR characteristics were investigated. Wallflower showed strong anti-inflammatory activity compared to diclofenac sodium. The root (1.25, 2.5, and 5 mg/mL) and flower (10 mg/mL) extract exhibited higher anti-inflammatory activities than that of other plant organs at the same concentrations. Moreover, total ash was found higher in aerial parts ( 21.52 ± 0.06 % ) than flower ( 11.01 ± 0.03 % ), root ( 5.03 ± 0.03 % ), and seed ( 6.95 ± 0.06 % ), while water-soluble ash was higher in seed ( 34.89 ± 0.26 % ) than flower ( 5.00 ± 0.03 % ), aerial parts ( 7.16 ± 0.06 % ), and root ( 5.04 ± 0.01 % ). Acid-insoluble ash and sulphated ash were higher in root ( 9.50 ± 0.04 % ) and aerial part ( 28.37 ± 0.57 % ), respectively. In addition, loss on drying was ranged from 2.20 ± 0.20 % in flowers to 6.00 ± 0.10 % in aerial parts. On the other hand, HPTLC analysis verified cardenolide compounds in all organs of the herb, and quercetin was detected in the flavonoid fingerprint of acid hydrolysed flowers. According to FT-IR results, the observed spectral region at ~3500 cm-1 attributed to -OH stretching vibration. Also, C–H (~2900-2950 cm-1), isothiocyanate (~2340 cm-1), -C=O (~1740 cm-1), conjugated C=C of the aromatic ring (~1650 cm-1), and structure of the aromatic group (~1200-1000 cm-1) were monitored. This work is the first study to the best of our knowledge, suggesting wallflower as a potential drug candidate with the basis for a monograph in addition to initial in vitro anti-inflammatory data.

scholarly journals Anti-Inflammatory Activity of Pleurotus ostreatus, a Culinary Medicinal Mushroom, in Wistar Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
W. J. A. Banukie N. Jayasuriya ◽  
Shiroma M. Handunnetti ◽  
Chandanie A. Wanigatunge ◽  
Gita H. Fernando ◽  
D. Thusitha U. Abeytunga ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Inflammatory Activity ◽  
No Production ◽  
Protective Effects ◽  
Paw Oedema ◽  
Freeze Dried ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Rat Paw Oedema

Context. Pleurotus ostreatus (P.o) is a culinary mushroom which is commonly called as “oyster mushroom” belonging to the Basidiomycetous fungi of the order Agaricales and family Pleurotaceae. Objectives. The present study investigates the anti-inflammatory potential of P.o and the underlying mechanisms of activity. Materials and Methods. Anti-inflammatory activity was evaluated using suspensions of freeze-dried and powdered (SFDP) P.o and acetone extract (AE) of P.o in normal and alloxan-induced diabetic rats using the carrageenan-induced rat paw oedema model. The mechanisms by which P.o is mediating the anti-inflammatory activity were studied using in vivo and in vitro assays. Results. At doses of 500–1000 mg/kg, the SFDP of P.o showed long-lasting activity at both early and late phases of carrageenan-induced rat paw oedema. The dose of 750 mg/kg showed the most potent inhibitory activity (92% inhibition) in healthy rats. The AE of P.o showed maximum inhibition of oedema of 87%. P.o exerted protective effects on the inflammatory pathologies in rats with diabetes. The possible mechanisms by which P.o mediates the anti-inflammatory activity were antihistamine activity (52.1%), inhibition of cell migration to the site of inflammation (45.4%), in vitro membrane stabilizing activity (52.6%), and inhibition of nitric oxide (NO) production (91.2%) (P<0.05). Dose-dependent inhibition of NO production was seen with in vitro treatment of rat peritoneal cells with AE of P.o (r = 0.95; P<0.05). Discussion and Conclusion. The promising activity of culinary mushroom P.o against inflammation suggests its potential application as a functional food during inflammatory conditions.

Phytochemical, Quantitative Proximate and In vitro Anti-inflammatory Study of the Crude Methanol Extract of Stachytarpheta indica Leaves (Verbenaceae)

2019 ◽  
pp. 153-162
Author(s):  
Cyril Ogbiko ◽  
Dickson Achimugu Musa ◽  
Musa Usman Dabai ◽  
Ibeabuchi Jude Ali ◽  
Abubakar Sani Yelwa ◽  
...  
Keyword(s):  
Cardiac Glycosides ◽  
Methanol Extract ◽  
Proximate Analysis ◽  
Water Soluble ◽  
Inflammatory Activity ◽  
Dependent Manner ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Crude Methanol Extract

Herbal medicine despite its increase usage globally is being plagued by inadequate research on their effectiveness. Stachytarpheta indica commonly known as snakeweed is used locally in the management of asthma, headache, alopecia, bronchitis, bruise, chest cold, constipation, itch, diarrhea, skin sore, vermifuge, dysentery, dysmenorrhea among others. This study investigated the phytochemical composition, quantitative proximate parameters and in vitro anti-inflammatory activity of the leaves of S. indica using established methods. Results confirmed the presence of alkaloids, saponins, carbohydrate, cardiac glycosides, terpenoid, tannin, anthraquinones, phenolics and flavonoid. Moisture content (9.16 ± 0.04%), total ash (19.42 ± 0.15%), water insoluble ash (7.17 ± 0.22%), acid insoluble ash (3.63 ± 0.26%), alcohol soluble extractive (0.99 ± 0.06%) and water-soluble extractive (0.36 ± 0.02%) values were obtained from the quantitative proximate analysis. An infusion of the methanol leaf extract of Stachytarpheta indica demonstrated significant anti-inflammatory activity in a dose dependent manner comparable to that of indomethacin standard.

scholarly journals In Vivo Wound Healing and In Vitro Anti-Inflammatory Activity Evaluation of Phlomis russeliana Extract Gel Formulations

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2695
Author(s):  
Mehmet Evren Okur ◽  
Ayşe Esra Karadağ ◽  
Neslihan Üstündağ Okur ◽  
Yağmur Özhan ◽  
Hande Sipahi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Activity ◽  
Wound Model ◽  
Aerial Parts ◽  
Excisional Wound ◽  
Anti Inflammatory ◽  
Cell Culture Assay ◽  
Gel Formulations

The air-dried aerial parts of Phlomis russeliana (Sims) Lag. Ex Benth. was extracted by methanol and fractionated by n-hexane, dichloromethane, and ethyl acetate, respectively. The wound healing properties of P. russeliana extract gel was evaluated using the in vivo excisional wound model using Balb-c mice. Initially, the P. russeliana methanol extract showed LOX inhibitory activity at IC50 = 23.2 µg/mL, whereas the DPPH• assay showed IC50 = 0.89 mg/mL, and the ABTS• assay showed IC50 = 0.99 mg/mL, respectively. In addition, a remarkable anti-inflammatory activity was observed in the cell culture assay. Thereafter, activity-guided fractionation was performed by LOX enzyme inhibition assays, and the structures of the two most active fractions were revealed by both GC–FID and GC/MS analyses, simultaneously. Phytol and 1-heptadecanoic acid were characterized as the active constituents. Moreover, the P. russeliana extract gel formulation was applied for in vivo tests, where the new gel formulation supported the in vitro anti-inflammatory activity findings. As a conclusion, this experimental results support the wound healing evidence based on the ethnobotanical application of Phlomis species with further potential.

scholarly journals In vitro anti-urease, antioxidant, anticholinesterase, cytotoxic and in vivo anti-inflammatory potential of Satureja cuneifolia Ten.

2020 ◽  
Vol 12 (2) ◽  
pp. 222-232
Author(s):  
Turgut TASKIN ◽  
Murat DOGAN ◽  
Muhammet E. CAM ◽  
Talip SAHIN ◽  
Ismail SENKARDES
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Cytotoxic Activities ◽  
Methanol Extracts ◽  
Aerial Parts ◽  
Direct Methanol ◽  
Anti Inflammatory

Satureja cuneifolia Ten. (wild savoury) belongs to the Lamiaceae family and is used to produce essential oil and aromatic water. This plant is also used as a condiment and herbal tea due to its stimulating, tonic and carminative effects. The in vitro antioxidant, anti-urease, anticholinesterase and cytotoxic activities of the different extracts from the plant’s aerial parts were examined. Besides, the in vivo anti-inflammatory activities of the fraction and direct methanol extracts were determined comparatively. In the current study, fraction methanol extract exhibited the strongest ABTS (52.34 mM trolox/mg extract) radical scavenging and ferric reduction (17.22 mM Fe2+/mg extract) activity. It was also found that the fraction methanol extract had stronger anti-urease (12.52%) and anticholinesterase (69.02%) activity than other extracts.  The XTT results showed that fraction methanol extract had the most cytotoxic activity on MCF-7 cell lines (39.92%).  According to the results of in vivo anti-inflammatory activity, it was found that both fraction and direct methanol extracts exhibited close and significant anti-inflammatory activity. The fact that methanol extracts have significant biological activity suggests that these may be used as a natural source in the future.

scholarly journals EFFECT OF DRYING TECHNIQUE ON PHYSICAL QUALITY AND ANTI-INFLAMMATORY ACTIVITY OF WILD BETEL LEAVES (Piper sarmentosum Roxb. ex Hunter) EXTRACT

2018 ◽  
Vol 16 (1) ◽  
pp. 42
Author(s):  
Fahleni Fahleni ◽  
Yuslia Noviani ◽  
Syarifah Ramadhani
Keyword(s):  
Water Content ◽  
Freeze Drying ◽  
Inflammatory Activity ◽  
Test Results ◽  
Drying Method ◽  
Flow Properties ◽  
Dry Extract ◽  
Freeze Dried ◽  
Anti Inflammatory ◽  
Dried Extract

Kaduk betel leaves (Piper sarmentosum Roxb. ex. Hunter) contain flavonoids and alkaloids that have anti-inflammatory activity. Processing thick extract into a dry extract is required to determine the appropriate dose and increase the homogeneity. The purpose of tahhis study was to determine the effect of drying method to physical properties of dried powder and its antiinflammatory activity. Thick extract was dried using maltodextrin and lactose as adsorbents with concentrations of extract-adsorbent ratio  1: 1, 1: 2 and 1: 3. Method of drying were freeze drying and adsorbent. The evaluation results based on organoleptic dried powder, water content and flow properties. The water content of dried powder which is dried by freeze drying is smaller than that of  the adsorbent. Organoleptic and flow properties of freeze dried powder results were also better. The best dried powder in each method tested its anti-inflammatory activity using BSA. The test results showed that the anti-inflammatory activity of dried extract of freeze drying (extract-maltodextrin 1:3)  have higher activity with IC50 551.79 ppm compared to dried extract with adsorbent (extract-maltodextrin 1:3) 598.30 ppm.

scholarly journals Phytochemical analysis, antioxidant, antidiabetic and anti-inflammatory activity of bark of Gardenia latifolia

2019 ◽  
Vol 9 (1) ◽  
pp. 141-145
Author(s):  
Farheen Ansari ◽  
Salaj Khare ◽  
B. K. Dubey ◽  
Amit Joshi ◽  
Amit Jain ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Dpph Radical ◽  
Hydroalcoholic Extract ◽  
Radical Method ◽  
Anti Inflammatory

Gardenia latifolia (Rubiaceae) is commonly known as Indian boxwood or Ceylon boxwood, is a densely foliaceous small tree that occurs throughout the greater parts of Indian common in deciduous forests along the streams. The stem bark and fruits are reported to be used in the treatment of various ailments such as snake bite, skin diseases, stomach pains, caries in humans and ephemeral fever in live stocks. Many beneficial medicinal products are used to treat various serious diseases and disorders like diabetes, cancer and cardiovascular diseases without side effects. Hence our study focused to investigate the phytochemical analysis, quantification of bioactive compounds, in vitro free radical scavenging activity (DPPH radical method), anti-inflammatory activity (Carrageenan induced hind paw oedema ), in vitro anti-diabetic activity by enzyme inhibition activity of bark of Gardenia latifolia (hydroalcoholic extract) which has boundless medicinal properties. The results of this study showed the evidence that the extracts when tested for their phytochemicals and free radical scavenging activity were found to have considerable antioxidant potential. This plant also exhibit better in vitro enzyme inhibitory activity and anti-inflammatory activity. The results of this study indicate that the hydroalcoholic extract of Gardenia latifolia bark has significant pharmacological properties. Keywords: Gardenia latifolia, Phytochemical analysis, DPPH radical method, Anti-inflammatory activity, Anti-diabetic activity

scholarly journals In vitro Anti-inflammatory Effect of Carthamus lanatus L.

2003 ◽  
Vol 58 (11-12) ◽  
pp. 830-832 ◽  
Author(s):  
Saima Jalil ◽  
Bozhanka Mikhova ◽  
Rilka Taskova ◽  
Maya Mitova ◽  
Helmut Duddeck ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Human Neutrophils ◽  
Inhibitory Effects ◽  
Aerial Parts ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Abstract The anti-inflammatory activity of four total extracts, their fractions and two main constituents (α-bisabolol β-ᴅ-fucopyranoside and luteolin 7-O-glucoside) of Carthamus lanatus L. aerial parts, were assessed in vitro by determining the inhibitory effects on induced human neutrophils. The dichloromethane extract and its water-alcoholic part exhibited the most significant inhibitory effects.

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