scholarly journals In-vitro evaluation of Nigella sativa and Punica granatum effect on protoscolices of hydatid cysts

2019 ◽  
Vol 28 (2) ◽  
pp. 210-214 ◽  
Author(s):  
Nasr Moawad El-Bahy ◽  
Amer Ragheb Abdelaziz ◽  
Reda Elbastawisy Khalafalla
Keyword(s):  
Anaphylactic Shock ◽  
Treatment Approach ◽  
Nigella Sativa ◽  
Punica Granatum ◽  
Hydatid Cysts ◽  
Hydroalcoholic Extract ◽  
Hydatid Fluid ◽  
Viability Assay ◽  
Peel Extract

Abstract Cystic echinococcosis (CE) are commonly found in the liver and lungs of affected hosts. The treatment approach is usually surgical, or giving drugs in conjunction before surgery to kill protoscolices, to avoid anaphylactic shock from leakage of hydatid fluid into the peritoneum and to decrease opportunities for recurrences. The present study was to evaluate the in vitro scolicidal efficacy of hydroalcoholic extract of Punica granatum peel and Nigella sativa, on the protoscolices of CE that collected from the lungs of infected camels. Different concentrations of extracts with different exposure times were used and a viability assay was applied to measure the scolicidal effect. N. sativa showed its highest scolicidal efficacy at 100 mg/mL and 10 mg/mL concentrations after 30 and 60 min. P. granatum peel extract showed its maximum scolicidal efficacy at 100 mg/mL concentration after 120 min. All experiments of the current study revealed that the extracts of both N. sativa and P. granatum had a scolicidal effects on the protoscolices of camel hydatid cysts. It could be concluded that N. sativa extract is more potent than P. granatum peel extract regarding scolicidal effect, but the efficacies of both extracts were of moderate significant correlation to exposure time and concentrations.

Biosynthesis of Silver Nanoparticles by Punica Granatum Peel Extract and their Biological Activity on different Pathogenic Bacteria

2021 ◽  
Vol 19 (9) ◽  
pp. 38-45
Author(s):  
Hussein H. Al-Turnachy ◽  
Fadhilk. alibraheemi ◽  
Ahmed Abd Alreda Madhloom ◽  
Zahraa Yosif Motaweq ◽  
Nibras Yahya Abdulla
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Biological Activity ◽  
Pathogenic Bacteria ◽  
Punica Granatum ◽  
Gram Positive ◽  
Gram Negative ◽  
Inhibition Zones ◽  
Peel Extract

The present study was included the assessment of the antimicrobial activity of AgNPs synthesized by Punica granatum peel extract against pathogenic bacteria by testing warm aqueous P. granatum peel extract and silver nanoparticles. Punica granatum indicated potency for AgNP extracellular nanobiosynthesis after addition of silver nitrate (AgNO3) 4mM to the extract supernatant, in both concentrations (100mg and 50mg). The biogenic AgNPs showed potency to inhibit both gram-negative and gram-positive bacterial growth. Zons of inhibition in (mm) was lesser in gram-positive than gram-negative bacteria. The resulted phytogenic AgNPs gave higher biological activity than warm aqueous Punica granatum peel extract. The inhibition zone of the phytogenic AgNPs on E. coli reached 17.53, 22.35, and 26.06 mm at (0.1, 0.5, and 1) mg/ml respectively. While inhibition zones of Punica warm aqueous extract reached 5.33, 10.63, and 16.08 mm at the same concentrations. phytogenic AgNPs gave smaller inhibition zones in gram-positive than gram- negative. Cytotoxic activity of the phytogenic AgNPs was assayed in vitro agaist human blood erythrocytes (RBCs), spectroscopic results showed absorbance at 540 nm hemolysis was observed. In general, AgNPs showed least RBCs hemolysis percentage, at 1 mg/ml concentration, hemolysis percentage was (4.50%). This study, concluded that the Punica granatum peel extract has the power of synthses of AgNPs characterized by broad spectrum antimicrobial activity with cyto-toxicity proportional to AgNPs concentration.

Antioxidant and anti-diabetic activity of pomegranate (Punica granatum L.) leaves extracts

2020 ◽  
Vol 8 (2) ◽  
pp. 329-336
Author(s):  
Mohammed Cheurfa ◽  
Mohamed Achouche ◽  
Ahmed Azouzi ◽  
Mariod A. Abdalbasit
Keyword(s):  
Hydrogen Peroxide ◽  
Aqueous Extract ◽  
Punica Granatum ◽  
Aqueous Extracts ◽  
Total Polyphenols ◽  
Hydroalcoholic Extract ◽  
Dpph Test ◽  
Total Antioxidant ◽  
Punica Granatum L

Introduction. This study aimed to evaluate the antioxidant and anti-diabetic activity of aqueous and hydroalcoholic extracts of pomegranate (Punica granatum L.) leaves in vitro, as well as to determine the content of polyphenols, flavonoids, and flavonols. Study objects and methods. The antioxidant activity was determined by the DPPH test using the free radical 1,1-diphenyl-2-picrylhydrazyle and the FRAP method, as well as by measuring total antioxidant capacity and the hydrogen peroxide scavenging activity. Results and discussion. The content of total polyphenols varied between 4.43 ± 0.3 and 12.66 ± 1.6 mg EAG/g. The highest content of flavonoids was observed in the hydroalcoholic extract of P. granatum leaves (P < 0.05). The flavonol contents in the hydroalcoholic and aqueous extracts were 7.68 ± 0.6 and 9.20 ± 2.8 mg EQ/g, respectively. The IC50 of the antioxidant potential of the hydroalcoholic and aqueous extracts was 32.4 ± 1.109 and 35.12 ± 4.107 mg/mL, respectively. According to the DPPH test, the aqueous extract was the least active (IC50 = 14.15 ± 1.513 mg/mL). The highest percentage of hydrogen peroxide trapping was found in the aqueous extract (45.97 ± 6.608 %). The inhibition of α-amylase showed an IC50 of between 9.804 ± 0.67 and 19.011 ± 9.82 mg/mL in the aqueous and hydroalcoholic extracts, respectively. The inhibition of glucose uptake by yeast recorded a high inhibitory capacity at 50 mg/mL of glucose. Conclusion. We found that the antioxidant and anti-diabetic activity of P. granatum leaves extracts was due to the presence of bioactive compounds such as flavonoids, which is why they are effective in preventing diabetes and its complications.

scholarly journals Alleviate Effect of Pomegranate Peel Extract in Ameliorating Fluoride-Induced Cytotoxicity, Oxidative Stress in Tetrahymena pyriformis Model

2021 ◽  
Vol 12 (3) ◽  
pp. 3710-3724
Keyword(s):  
Oxidative Stress ◽  
Mammalian Cells ◽  
Antioxidant Properties ◽  
Tetrahymena Pyriformis ◽  
Punica Granatum ◽  
Redox Status ◽  
Total Phenolic ◽  
Pomegranate Peel ◽  
Peel Extract

Fluoride is a major oligo element found in nature, at excessive amounts can cause enormous harm in mammalian cells. Fruits peel, considered most often as a waste of juice processing, could play an important role in attenuating metal cytotoxicity. The present study evaluated the effect of pomegranate peel (Punica granatum. L) methanolic extract (PPE) on the Fluoride-induced toxicity and redox status in the protozoa Tetrahymena pyriformis. Polyphenols of peel extract were extracted using methanol and characterized by spectrophotometric methods, total phenolic content (TPC), total flavonoids content (TFC), and in vitro, antioxidant properties were assessed using the Folin-Ciocalteu method and DPPH, ABTS, and FRAP. Pomegranate peel is a rich source of phenolic compounds TP (223.21 ± 15 mg GAE/g dw), TF (52.12 ± 1.36 mg Qu/g dw) and showed high antioxidant properties DPPH (EC50 0.043 ± 0.06 mg/ml), ABTS (EC50 0.06 ± 0.01 mg/ml) and FRAP (1.47 ± 0.01 mg AA equivalents/g dw). Cells were incubated with fluoride alone and in combination with PPE. NaF (0.8 mM) significantly decreased the cell viability, induced oxidative stress by decreasing antioxidants enzyme activities, and increased intracellular fluoride content. Treatment with NaF in combination with PPE decreases CAT, SOD, and GPx activities and alleviates GSH content. These findings suggest that pomegranate peel biomolecules may have a protective effect against fluoride induced-toxicity.

scholarly journals Anti-infective potential of hydroalcoholic extract of Punica granatum peel against gram-negative bacterial pathogens

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 70 ◽  
Author(s):  
Chinmayi Joshi ◽  
Pooja Patel ◽  
Vijay Kothari
Keyword(s):  
Traditional Medicine ◽  
Pathogenic Bacteria ◽  
Scientific Evidence ◽  
Punica Granatum ◽  
Bifidobacterium Bifidum ◽  
Haemolytic Activity ◽  
Hydroalcoholic Extract ◽  
Pre Treatment

Background: Punica granatum extracts have been prescribed in traditional medicine for management of a variety of disease conditions including microbial infections. Generation of scientific evidence for validation of P. granatum peel extract’s anti-pathogenic efficacy is required. Methods: Hydroalcoholic extract of P. granatum peel (PGPE), prepared by microwave assisted extraction method was evaluated for its quorum-modulatory potential against two different human-pathogenic bacteria viz. Chromobacterium violaceum and Pseudomonas aeruginosa. Results: This extract was able to modulate in vitro production of quorum sensing-regulated pigments in both these test bacteria at ≥5 μg/ml. Virulence traits of P. aeruginosa like haemolytic activity, and biofilm formation were negatively affected by the test extract, and it also made P. aeruginosa more susceptible to lysis by human serum. Antibiotic susceptibility of both test bacteria was modulated owing to pre-treatment with PGPE. Exposure of these test pathogens to PGPE (≥0.5 μg/ml) effectively reduced their virulence towards the nematode Caenorhabditis elegans. Repeated subculturing of P. aeruginosa on PGPE-supplemented growth medium did not induce resistance to PGPE in this notorious pathogen, and this extract was also found to exert a post-extract effect on P. aeruginosa. Individual constituent phytocompounds of PGPE were found to be less efficacious than the whole extract. PGPE seemed to interfere with the signal-response machinery of P. aeruginosa and C. violaceum. PGPE also exhibited notable prebiotic potential by promoting growth of probiotic strains- Bifidobacterium bifidum and Lactobacillus plantarum at ≤50 μg/ml. Conclusions: This study indicates PGPE to be an effective antipathogenic and prebiotic preparation, and validates its therapeutic use mentioned in traditional medicine. This study also emphasizes the need for testing any bioactive extract at broadest possible concentration range, particularly in vivo, so that an accurate picture of dose-response relationship can emerge.

scholarly journals In Vitro and in Vivo Scolicidal Activities of Holothuria leuco-spilota Extract and CeO2 Nanoparticles against Hydatid Cyst

2019 ◽  
Author(s):  
Safa ARYAMAND ◽  
Shahram KHADEMVATAN ◽  
Khosrow HAZRATI TAPPEH ◽  
Behnam HESHMATIAN ◽  
Ali JELODAR
Keyword(s):  
Hydatid Cyst ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Ceo2 Nanoparticles ◽  
Hydatid Cysts ◽  
Hydatid Fluid ◽  
Cyst Development

Background: We aimed to investigate the scolicidal effects of Holothuria leucospilota extract and CeO2 nanoparticles against protoscoleces of hydatid cysts in-vitro and in-vivo. Methods: Hydatid cysts were collected from, Urmia slaughterhouses between years 2016-2017 and the hydatid fluid aspirated from the fertile cysts. Various concentration of H. leucospilota extract, CeO2 NPs and combination of CeO2-NPs/H. leucospilota were used for 10-60 min to evaluate the viability of protoscoleces by 0.1% eosin method. CASPASE -3 activity measured for assessment of cell apoptosis in treated protoscoleces. BALB/c mice were infected intraperitoneally with 2000 viable protoscoleces and treated daily for 4 wk by intragastrical inoculation with H. leucospilota, CeO2 NPs, combination of CeO2 NPs/H. leucospilota and Albendazole. Cyst development was macroscopically analyzed. Results: H. leucospilota extract and combination of CeO2 NPs/H. leucospilota have potent scolicidal activity at concentration of 20 mg/ml and 15 mg/ml after 60 min treatment. Maximum caspase-3 activity was observed when protoscoleces expose with H. leucospilota and combination H. leucospilota & CeO2 NPs. After treatment of cyst infected mice with extract and CeO2 NPs, combination of CeO2 NPs/H leucospilota and albendazole, a significant decrease in number of cysts, size and volume of cyst (P<0.05) was observed. Conclusion: This result shows an antihydatic and scolicidal effects of H. leucospilota extract and CeO2 NPs.

scholarly journals Anti-infective potential of hydroalcoholic extract of Punica granatum peel against gram-negative bacterial pathogens

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 70 ◽  
Author(s):  
Chinmayi Joshi ◽  
Pooja Patel ◽  
Vijay Kothari
Keyword(s):  
Traditional Medicine ◽  
Pathogenic Bacteria ◽  
Scientific Evidence ◽  
Punica Granatum ◽  
Bifidobacterium Bifidum ◽  
Haemolytic Activity ◽  
Hydroalcoholic Extract ◽  
Pre Treatment

Background: Punica granatum extracts have been prescribed in traditional medicine for management of a variety of disease conditions including microbial infections. Generation of scientific evidence for validation of P. granatum peel extract’s anti-pathogenic efficacy is required. Methods: Hydroalcoholic extract of P. granatum peel (PGPE), prepared by microwave assisted extraction method was evaluated for its quorum-modulatory potential against two different human-pathogenic bacteria viz. Chromobacterium violaceum and Pseudomonas aeruginosa. Results: This extract was able to modulate in vitro production of quorum sensing-regulated pigments in both these test bacteria at ≥5 μg/ml. Virulence traits of P. aeruginosa like haemolytic activity, and biofilm formation were negatively affected by the test extract, and it also made P. aeruginosa more susceptible to lysis by human serum. Antibiotic susceptibility of both test bacteria was modulated owing to pre-treatment with PGPE. Exposure of these test pathogens to PGPE (≥0.5 μg/ml) effectively reduced their virulence towards the nematode Caenorhabditis elegans. Repeated subculturing of P. aeruginosa on PGPE-supplemented growth medium did not induce resistance to PGPE in this notorious pathogen, and this extract was also found to exert a post-extract effect on P. aeruginosa. Individual constituent phytocompounds of PGPE were found to be less efficacious than the whole extract. PGPE seemed to interfere with the signal-response machinery of P. aeruginosa and C. violaceum. PGPE also exhibited notable prebiotic potential by promoting growth of probiotic strains- Bifidobacterium bifidum and Lactobacillus plantarum at ≤50 μg/ml. Conclusions: This study indicates PGPE to be an effective antipathogenic and prebiotic preparation, and validates its therapeutic use mentioned in traditional medicine. This study also emphasizes the need for testing any bioactive extract at broadest possible concentration range, particularly in vivo, so that an accurate picture of dose-response relationship can emerge.

scholarly journals ẢNH HƯỞNG CỦA DỊCH TRÍCH VỎ QUẢ LỰU (Punica granatum) LÊN SỰ ỨC CHẾ HÌNH THÀNH TINH THỂ CALCIUM OXALATE GÂY BỆNH SỎI THẬN TRONG ĐIỀU KIỆN IN VITRO

2020 ◽  
Vol 4 (3) ◽  
pp. 2095-2103
Author(s):  
Nguyễn Phạm Tuấn ◽  
Bằng Hồng Lam ◽  
Nguyễn Phạm Tú ◽  
Lê Thảo Nguyên ◽  
Trần Đức Tài
Keyword(s):  
Calcium Oxalate ◽  
Punica Granatum ◽  
Pomegranate Peel ◽  
Aggregation Assay ◽  
Ic50 Value ◽  
Growth Assay ◽  
Pomegranate Peel Extract ◽  
Peel Extract ◽  
Nucleation Growth

Nghiên cứu sử dụng dịch trích vỏ quả lựu được thực hiện để đánh giá khả năng ức chế tinh thể Calcium oxalate, gồm 03 giai đoạn chính là hình thành, phát triển và ngưng tụ. Mẫu vỏ quả lựu được ly trích bằng phương pháp ngâm dầm với ethanol 80% để tạo cao chiết. Phần trăm ức chế hạt nhân tinh thể Calcium oxalate của cao chiết vỏ quả lựu được xác định bằng phương pháp đo quang phổ ở bước sóng 620 nm; trong khi đó, hiệu quả ức chế phát triển tinh thể Calcium oxalate của cao chiết được đánh giá bằng mật độ quang của mẫu thử ở bước sóng 214 nm trong thời gian 600 giây. Hiệu quả ức chế ngưng tụ tinh thể calcium oxalate của cao chiết được xác định bằng cách đo lường mật độ quang ở bước sóng 620 nm vào các khoảng thời gian 30, 60, 90, 180 và 360 phút. Kết quả nghiên cứu cho thấy, độ ẩm của mẫu đạt 71,89% và hiệu suất cao chiết đạt 4,59%. Cao chiết vỏ quả lựu có sự hiện diện của các hợp chất flavonoid, alkaloid, saponin, terpenoid, tanin và phenol. Cao chiết vỏ quả lựu có khả năng ức chế hình thành hạt nhân, phát triển và ngưng tụ của tinh thể Calcium oxalate với giá trị IC50 lần lượt là 0,76 mg/mL; 0,75 mg/mL và 0,99 mg/mL. ABSTRACT The study on using Pomegranate peel extract to inhibit the formation of Calcium oxalate, including three main phases: nucleation, growth, and aggregation was conducted. The plant samples were extracted from maceration method with 80% of ethanol. The inhibitory percentage of nucleation was determined by spectrophotometer at 620 nm, whereas the growth assay was evaluated by measuring of sample in 600 seconds at wavelength 214 nm. The aggregation assay was conducted by measuring at the period of 30, 60, 90, 180, and 360 minutes at wavelength 620 nm to determine the inhibitory percentage. The results showed that moisture and the yield of Pomegranate peel extract were 71.89% and 4.59%. The extract of Pomegranate peel had the presence of bioactive compounds such as alkaloid, flavonoid, saponin, terpenoid, tannin and phenol. The extract Punica granatum peel had the ability to inhibit nucleation, growth and aggregation of Calcium oxalate crystallisation and IC50 value of extract was 0.76 mg/mL, 0.75 mg/mL, and 0.99 mg/mL, respectively.  

Efficacy of Punica granatum extract on in vitro and in vivo control of Trichomonas vaginalis

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
GT El Sherbini ◽  
KM Ibrahim ◽  
ET El Sherbini ◽  
NM Abdel Hady ◽  
TA Morsy
Keyword(s):  
Trichomonas Vaginalis ◽  
Punica Granatum
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