Increased urinary levels of pentosidine measured by a newly developed ELISA are independently correlated with fracture after fall

Polychlorinated biphenyls (PCBs) are a ubiquitous class of environmental pollutants with toxic and hepatocellular effects, including accumulation of fat, proliferated smooth endoplasmic recticulum (SER), and concentric membrane arrays (CMAs) (1-3). The CMAs appear to be a membrane storage and degeneration organelle composed of a large number of concentric membrane layers usually surrounding one or more lipid droplets often with internalized membrane fragments (3). The present study documents liver alteration after a short term single dose exposure to PCBs with high chlorine content, and correlates them with reported animal weights and central nervous system (CNS) measures. In the brain PCB congeners were concentrated in particular regions (4) while catecholamine concentrations were decreased (4-6). Urinary levels of homovanillic acid a dopamine metabolite were evaluated (7).Wistar rats were gavaged with corn oil (6 controls), or with a 1:1 mixture of Aroclor 1254 and 1260 in corn oil at 500 or 1000 mg total PCB/kg (6 at each level).

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Faculty Opinions recommendation of Urinary levels of TGF β-1 and of cytokines in patients with prenatally detected nephrouropathies.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.10209956.11001054 ◽

2011 ◽

Author(s):

Jean Hollowell

Keyword(s):

Urinary Levels

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Urinary Cytokines Reflect Renal Inflammation in Acute Tubulointerstitial Nephritis: A Multiplex Bead-Based Assay Assessment

Journal of Clinical Medicine ◽

10.3390/jcm10132986 ◽

2021 ◽

Vol 10 (13) ◽

pp. 2986

Author(s):

Laura Martinez Valenzuela ◽

Juliana Draibe ◽

Oriol Bestard ◽

Xavier Fulladosa ◽

Francisco Gómez-Preciado ◽

...

Keyword(s):

Tubulointerstitial Nephritis ◽

Urinary Concentration ◽

Blood Eosinophil ◽

Acute Tubulointerstitial Nephritis ◽

Luminex Assay ◽

Non Invasive ◽

Urinary Levels ◽

Histological Data ◽

The Individual ◽

Multiplex Bead

Background: Acute tubulointerstitial nephritis (ATIN) diagnosis lays on histological assessment through a kidney biopsy, given the absence of accurate non-invasive biomarkers. The aim of this study was to evaluate the accuracy of different urinary inflammation-related cytokines for the diagnostic of ATIN and its distinction from acute tubular necrosis (ATN). Methods: We included 33 patients (ATIN (n = 21), ATN (n = 12)), and 6 healthy controls (HC). We determined the urinary levels of 10 inflammation-related cytokines using a multiplex bead-based Luminex assay at the time of biopsy and after therapy, and registered main clinical, analytical and histological data. Results: At the time of biopsy, urinary levels of I-TAC/CXCL11, CXCL10, IL-6, TNFα and MCP-1 were significantly higher in ATIN compared to HC. A positive correlation between the extent of the tubulointerstitial cellular infiltrates in kidney biopsies and the urinary concentration of I-TAC/CXCL11, MIG/CXCL9, CXCL10, IL17, IFNα, MCP1 and EGF was observed. Notably, I-TAC/CXCL11, IL-6 and MCP-1 were significantly higher in ATIN than in ATN, with I-TAC/CXCL11 as the best discriminative classifier AUC (0.77, 95% CI 0.57–0.95, p = 0.02). A combinatory model of these three urinary cytokines increased the accuracy in the distinction of ATIN/ATN compared to the individual biomarkers. The best model resulted when combining the three cytokines with blood eosinophil and urinary leukocyte counts (LR = 9.76). Follow-up samples from 11ATIN patients showed a significant decrease in I-TAC/CXCL11, MIG/CXCL9 and CXCL10 levels. Conclusions: Urinary I-TAC/CXCL11, CXCL10, IL6 and MCP-1 levels accurately distinguish patients developing ATIN from ATN and healthy individuals and may serve as novel non-invasive biomarkers in this disease.

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Association between YAP expression in neoplastic and non-neoplastic breast tissue with arsenic urinary levels

Journal of Applied Toxicology ◽

10.1002/jat.3481 ◽

2017 ◽

Vol 37 (10) ◽

pp. 1195-1202 ◽

Cited By ~ 2

Author(s):

Gladis Michel-Ramirez ◽

Rogelio Recio-Vega ◽

Guadalupe Ocampo-Gomez ◽

Eduardo Palacios-Sanchez ◽

Manuel Delgado-Macias ◽

...

Keyword(s):

Breast Tissue ◽

Urinary Levels

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Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody

Scientific Reports ◽

10.1038/s41598-021-92934-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yoshihiro Osawa ◽

Kaho Nomura ◽

Yoshifumi Kimira ◽

Seiji Kushibe ◽

Ken-ichi Takeyama ◽

...

Keyword(s):

Physical Activity ◽

Student Athletes ◽

Cross Reactivity ◽

University Student ◽

Collagen Metabolism ◽

The Novel ◽

Urinary Level ◽

Collagen Peptide ◽

Urinary Levels ◽

Over Time

AbstractActive collagen oligopeptides (ACOP) are bioactive collagen-derived peptides detected by a recently-established ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody used in this ELISA. We then investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC–MS/MS, and ELISA. We observed that the antibody showed strong cross-reactivity to Pro-Hyp and Gly-Pro-Hyp and weak cross-reactivity to commercial CP. CP ingestion increased the urinary level of ACOP over time, which correlated highly with urinary levels of peptide forms of Hyp and Pro-Hyp. Physical activity significantly decreased the urinary ACOP level. This study demonstrates changes in urinary ACOP following oral CP intake and physical activity using ELISA with the novel anti-ACOP antibody. Thus, ACOP may be useful as a new biomarker for collagen metabolism.

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