Biphasic Calcium Phosphate Microparticles for Bone Formation: Benefits of Combination with Blood Clot

2010 ◽  
Vol 16 (11) ◽  
pp. 3495-3505 ◽  
Author(s):  
Thierry Balaguer ◽  
Florian Boukhechba ◽  
Arnaud Clavé ◽  
Sébastien Bouvet-Gerbettaz ◽  
Christophe Trojani ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Biphasic Calcium Phosphate ◽  
Blood Clot

scholarly journals Evaluation of Osteoconduction of Biphasic Calcium Phosphate Ceramic in the Calvaria of Rats: Microscopic and Histometric Analysis

2019 ◽  
Vol 10 (1) ◽  
pp. 7 ◽  
Author(s):  
Igor Puttini ◽  
Pier Poli ◽  
Carlo Maiorana ◽  
Igor Vasconcelos ◽  
Luis Schmidt ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Biphasic Calcium Phosphate ◽  
Blood Clot ◽  
Experimental Period ◽  
Male Rats ◽  
Collagen Membrane ◽  
New Bone Formation ◽  
Calvarial Bone ◽  
Percentage Area

(1) Background: Evaluate the osteoconduction capability of a biphasic calcium phosphate (BCP) ceramic composed of hydroxyapatite and β-tricalcium phosphate 60%/40% in a rat model. (2) Methods: In the calvarial bone of 54 adult male rats, 7-mm diameter critical size defects were performed. The animals were randomly allocated to three experimental groups according to the type of material: blood clot (BCG), blood clot covered with a bovine-derived collagen membrane (MBCG), and BCP ceramic covered with a bovine-derived collagen membrane (BCPG). In each group, 6 animals were euthanatized at post-operative days 7, 30, and 60 for histological and histometric analysis. (3) Results: The qualitative analysis revealed the persistence of the collagen membrane at seven days, with no relevant newly bone formation in all groups. At 30 days, centripetal bone formation was observed residual particles of the biomaterial surrounded by fibroblasts noted in the BCPG. At 60 days, while BCG and MBCG showed a partial maturation with the central part of the defect populated by a fibrous connective tissue, in the BCPG the critical area was entirely occupied by newly formed bone. In the intra groups analysis was noted a significant increase in new bone formation during the experimental period (p < 0.05). At 60 days, BCPG showed a higher percentage area of new bone formation (p < 0.05). (4) Conclusion: BCP promoted a new bone formation by osteoconduction and might be considered a valid alternative in bone regeneration procedures.

Fabrication of Biphasic Calcium Phosphate Foam Granular and its Initial Evaluation Using Beagle Dogs

2008 ◽  
Vol 396-398 ◽  
pp. 233-236
Author(s):  
Ishikawa Kunio ◽  
Ryouji Shimogoryo ◽  
Hanae Wakae ◽  
Akari Takeuchi ◽  
Shigeki Matsuya
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Biphasic Calcium Phosphate ◽  
Blood Clot ◽  
Formation Rate ◽  
Bone Defects ◽  
Carbonate Apatite ◽  
Beagle Dogs ◽  
Bone Formation Rate ◽  
Reconstruction Rate

Biphasic calcium phosphate foam consisting of core aTCP covered with carbonate apatite (CO3Ap) was fabricated based on the compositional transformation by exposing aTCP foam to (NH4)2HPO4 aqueous solution at 200 °C for 90min in order to understand the effect on bone formation rate and granule remaining ratio. The BCP foam was gently crushed and sieved to prepare foam granular. Bone defects made at the mandible of beagle dog were reconstructed with aTCP, BCP and CO3Ap foam granular. 3 months after surgery, bone reconstruction rate of the BCP and CO3Ap were 159.7±28.6% and 169.0±29.7% whereas those of aTCP and blood clot group were 89.5±14.7% and 85.2±13.2%, respectively, and no further increase was observed at 6 month. On the other hand, granular remaining ratio was 3.0±1.7%, 3.1±1.8%, 6.9±2.0% for BCP, aTCP and CO3Ap foam granular, respectivly. We concluded therefore, BCP foam granular could be an ideal bone substitute since it alllows large porosity, at least similar bone formation ratio to CO3Ap and faster replacement to bone.

Bone Marrow Autograft Associated to Macroporous Biphasic Calcium Phosphate for Bone Substitution in an Animal Model of Sequels of Radiotherapy

2005 ◽  
Vol 284-286 ◽  
pp. 285-288
Author(s):  
Oliver Malard ◽  
Jean Michel Bouler ◽  
Jerome Guicheux ◽  
Olivier Gauthier ◽  
E. Lerouxel ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Calcium Phosphate ◽  
Bone Formation ◽  
Biphasic Calcium Phosphate ◽  
Bone Repair ◽  
Major Surgery ◽  
Graft Versus Host Reaction ◽  
Bone Reconstruction ◽  
New Bone Formation ◽  
Study Results

Bone invasion is common in case of Squamous Cell Carcinomas (SCC) of the upper aero-digestive tract. Radiotherapy is required in addition to large surgical tumor removal. This treatment usually generates irreversible injuries on the reparation properties of the tissues, especially on bone. The quality of life of patients undergoing major surgery and radiotherapy in maxillary and mandible areas is reduced, but could be improved by bone reconstruction. The aim of this study was to evaluate the bone reconstruction possibilities by Macroporous Biphasic Calcium-Phosphate (MBCPÔ). The MBCP substitute was evaluated as granules and associated to autologous bone marrow (BM) graft in irradiated areas, in an inbreeding rodent model. Radiation sequels were created on inferior members of half of the rats. 3 weeks later, 3-mm osseous defects were created on each animal. The inbreeding model allows BM to be grafted without graft-versus-host reaction. Defects were filled either with MBCP alone, BM alone or a mixture of MBCP and BM. Six weeks after implantation, animals were sacrificed: bone repair and ceramic degradation were evaluated by qualitative and quantitative study. Results showed that bioceramics were well osteointegrated. Filling the defects with BM alone showed a significant increased of newly-formed bone formation but only after irradiation, whereas filling defects with MBCP alone increased new-bone formation only without previous irradiation. Associating MBCP to BM provided the best new-bone formation rates after irradiation. Degradation of the ceramic was the most important in case of BM grafting. This study demonstrated that BM added to MBCP constitute an appropriate material to be considered in case of bone defect occurring in irradiated tissue, and could be foreseen for use after bone removal for oncologic obligations.

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