Immunization with DNA Vaccines Containing Porcine Reproductive and Respiratory Syndrome Virus Open Reading Frames 5, 6, and 7 May Be Related to the Exacerbation of Clinical Disease after an Experimental Challenge

2013 ◽  
Vol 26 (1) ◽  
pp. 93-101 ◽  
Author(s):  
Ivan Díaz ◽  
Llilianne Ganges ◽  
Iván Galindo-Cardiel ◽  
Joan Tarradas ◽  
Belén Álvarez ◽  
...  
Keyword(s):  
Dna Vaccines ◽  
Open Reading Frames ◽  
Clinical Disease ◽  
Respiratory Syndrome Virus ◽  
Reading Frames ◽  
Experimental Challenge

scholarly journals Complete Genome Sequence of an NADC30-Like Porcine Reproductive and Respiratory Syndrome Virus Characterized by Recombination with Other Strains

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Yingying Li ◽  
Guobiao Ji ◽  
Juan Wang ◽  
Feifei Tan ◽  
Jinshan Zhuang ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Open Reading Frames ◽  
Respiratory Syndrome Virus ◽  
Reading Frames

We report here the complete genome sequence of an NADC30-like porcine reproductive and respiratory syndrome virus (PRRSV), HNyc15, which was characterized by recombination with VR-2332 and CH-1a PRRSV strains in open reading frames (ORFs) 2 to 4.

Porcine bocaviruses: genetic analysis and prevalence in Chinese swine population

2011 ◽  
Vol 139 (10) ◽  
pp. 1581-1586 ◽  
Author(s):  
H. B. ZHANG ◽  
L. HUANG ◽  
Y. J. LIU ◽  
T. LIN ◽  
C. Q. SUN ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Open Reading Frames ◽  
Porcine Circovirus ◽  
Respiratory Syndrome Virus ◽  
Mixed Infections ◽  
Detection Rates ◽  
Swine Population ◽  
Swine Herds ◽  
Reading Frames

SUMMARYIn members of theBocavirusgenus, that contain three open reading frames (ORFs) of the Parvovirinae subfamily, porcine bocaviruses (PoBoVs) exhibit the most genetic diversity. Based on the ORF2-encoded viral protein (VP1) classification, the six reported porcine bocaviruses were grouped into four species: PoBoV1 (porcine boca-like virus or PBoLV), PoBoV2 (porcine parvovirus 4 or PPV4), PoBoV3 (PBoV1/PBoV2) and PoBoV4 (6V/7V), with PoBoV3 and PoBoV4 each having two genotype viruses. All four PoBoV species were detected in the 166 samples collected in 2010 from swine herds located in ten provinces of China. The detection rates for PoBoV1-4 were 28·9%, 6·6%, 19·3% and 39·7%, respectively. The co-infection combinations involving these six porcine bocaviruses in the collected samples were very complex. Furthermore, mixed infections with viruses from other families (porcine reproductive and respiratory syndrome virus, classic swine fever virus and porcine circovirus type 2) were also detected.

Evaluation of protective effect of DNA vaccines encoding the BAB1_0263 and BAB1_0278 open reading frames of Brucella abortus in BALB/c mice

Vaccine ◽  
2012 ◽  
Vol 30 (50) ◽  
pp. 7286-7291 ◽  
Author(s):  
Fernanda Sislema-Egas ◽  
Sandra Céspedes ◽  
Pablo Fernández ◽  
Angello Retamal-Díaz ◽  
Darwin Sáez ◽  
...  
Keyword(s):  
Protective Effect ◽  
Brucella Abortus ◽  
Dna Vaccines ◽  
Open Reading Frames ◽  
Reading Frames

Relative contribution of porcine reproductive and respiratory syndrome virus open reading frames 2–4 to the induction of protective immunity

Vaccine ◽  
2017 ◽  
Vol 35 (34) ◽  
pp. 4408-4413 ◽  
Author(s):  
Kathryn Kimpston-Burkgren ◽  
Ignacio Correas ◽  
Fernando A. Osorio ◽  
David Steffen ◽  
Asit K. Pattnaik ◽  
...  
Keyword(s):  
Protective Immunity ◽  
Open Reading Frames ◽  
Respiratory Syndrome Virus ◽  
Relative Contribution ◽  
Reading Frames

scholarly journals Multivalent Fusion DNA Vaccine against Brucella abortus

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Leonardo Gómez ◽  
Javiera Llanos ◽  
Emilia Escalona ◽  
Darwin Sáez ◽  
Francisco Álvarez ◽  
...  
Keyword(s):  
Immune Response ◽  
Brucella Abortus ◽  
Dna Vaccines ◽  
Open Reading Frames ◽  
T Helper 1 ◽  
Lymphoproliferative Response ◽  
Low Levels ◽  
Prevention Method ◽  
Cellular Immune ◽  
Reading Frames

As an alternative brucellosis prevention method, we evaluated the immunogenicity induced by new multivalent DNA vaccines in BALB/c mice. We constructed the vaccines by fusion of BAB1_0273 and/or BAB1_0278 open reading frames (ORFs) from genomic island 3 (GI-3) and the Brucella abortus 2308 sodC gene with a link based on prolines and alanines (pV273-sod, pV278-sod, and pV273-278-sod, resp.). Results show that immunization with all tested multivalent DNA vaccines induced a specific humoral and cellular immune response. These novel multivalent vaccines significantly increased the production of IgM, IgG, and IgG2a antibodies as well as IFN-γ levels and the lymphoproliferative response of splenocytes. Although immunization with these multivalent vaccines induced a typical T-helper 1- (Th1-) dominated immune response, such immunogenicity conferred low protection levels in mice challenged with the B. abortus 2308 pathogenic strain. Our results demonstrated that the expression of BAB1_0273 and/or BABl_0278 antigens conjugated to SOD protein can polarize mice immunity to a Th1-type phenotype, conferring low levels of protection.

scholarly journals Immunisation of pigs with a major envelope protein sub-unit vaccine against porcine reproductive and respiratory syndrome virus (PRRSV) results in enhanced clinical disease following experimental challenge

2011 ◽  
Vol 189 (3) ◽  
pp. 323-329 ◽  
Author(s):  
Cinta Prieto ◽  
Francisco Javier Martínez-Lobo ◽  
Francisco Díez-Fuertes ◽  
Patricia Aguilar-Calvo ◽  
Isabel Simarro ◽  
...  
Keyword(s):  
Envelope Protein ◽  
Clinical Disease ◽  
Respiratory Syndrome Virus ◽  
Experimental Challenge
Sign in / Sign up

Export Citation Format

Share Document