scholarly journals Son Is Essential for Nuclear Speckle Organization and Cell Cycle Progression

2010 ◽  
Vol 21 (4) ◽  
pp. 650-663 ◽  
Author(s):  
Alok Sharma ◽  
Hideaki Takata ◽  
Kei-ichi Shibahara ◽  
Athanasios Bubulya ◽  
Paula A. Bubulya
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Mrna Processing ◽  
Mrna Splicing ◽  
Scaffolding Protein ◽  
Splicing Factors ◽  
Nuclear Speckles ◽  
Intact Cells ◽  
Cycle Progression ◽  
Processing Factors

Subnuclear organization and spatiotemporal regulation of pre-mRNA processing factors is essential for the production of mature protein-coding mRNAs. We have discovered that a large protein called Son has a novel role in maintaining proper nuclear organization of pre-mRNA processing factors in nuclear speckles. The primary sequence of Son contains a concentrated region of multiple unique tandem repeat motifs that may support a role for Son as a scaffolding protein for RNA processing factors in nuclear speckles. We used RNA interference (RNAi) approaches and high-resolution microscopy techniques to study the functions of Son in the context of intact cells. Although Son precisely colocalizes with pre-mRNA splicing factors in nuclear speckles, its depletion by RNAi leads to cell cycle arrest in metaphase and causes dramatic disorganization of small nuclear ribonuclear protein and serine-arginine rich protein splicing factors during interphase. Here, we propose that Son is essential for appropriate subnuclear organization of pre-mRNA splicing factors and for promoting normal cell cycle progression.

scholarly journals Genetic and Physical Interactions Between Factors Involved in Both Cell Cycle Progression and Pre-mRNA Splicing inSaccharomyces cerevisiae

Genetics ◽  
2000 ◽  
Vol 156 (4) ◽  
pp. 1503-1517 ◽  
Author(s):  
Sigal Ben-Yehuda ◽  
Ian Dix ◽  
Caroline S Russell ◽  
Margaret McGarvey ◽  
Jean D Beggs ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Synthetic Lethality ◽  
Mrna Splicing ◽  
Splicing Factors ◽  
Restrictive Temperature ◽  
Cycle Progression ◽  
Cellular Processes ◽  
Physical Interactions ◽  
Mutant Cells

AbstractThe PRP17/CDC40 gene of Saccharomyces cerevisiae functions in two different cellular processes: pre-mRNA splicing and cell cycle progression. The Prp17/Cdc40 protein participates in the second step of the splicing reaction and, in addition, prp17/cdc40 mutant cells held at the restrictive temperature arrest in the G2 phase of the cell cycle. Here we describe the identification of nine genes that, when mutated, show synthetic lethality with the prp17/cdc40Δ allele. Six of these encode known splicing factors: Prp8p, Slu7p, Prp16p, Prp22p, Slt11p, and U2 snRNA. The other three, SYF1, SYF2, and SYF3, represent genes also involved in cell cycle progression and in pre-mRNA splicing. Syf1p and Syf3p are highly conserved proteins containing several copies of a repeated motif, which we term RTPR. This newly defined motif is shared by proteins involved in RNA processing and represents a subfamily of the known TPR (tetratricopeptide repeat) motif. Using two-hybrid interaction screens and biochemical analysis, we show that the SYF gene products interact with each other and with four other proteins: Isy1p, Cef1p, Prp22p, and Ntc20p. We discuss the role played by these proteins in splicing and cell cycle progression.

scholarly journals Functional analyses of interacting factors involved in both pre-mRNA splicing and cell cycle progression in Saccharomyces cerevisiae

RNA ◽  
2000 ◽  
Vol 6 (11) ◽  
pp. 1565-1572 ◽  
Author(s):  
CAROLINE S. RUSSELL ◽  
SIGAL BEN-YEHUDA ◽  
IAN DIX ◽  
MARTIN KUPIEC ◽  
JEAN D. BEGGS
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Mrna Splicing ◽  
Cycle Progression ◽  
Functional Analyses

scholarly journals Sequential Entry of Components of Gene Expression Machinery into Daughter Nuclei

2003 ◽  
Vol 14 (3) ◽  
pp. 1043-1057 ◽  
Author(s):  
Kannanganattu V. Prasanth ◽  
Paula A. Sacco-Bubulya ◽  
Supriya G. Prasanth ◽  
David L. Spector
Keyword(s):  
Gene Expression ◽  
Rna Polymerase Ii ◽  
Mrna Processing ◽  
Mrna Splicing ◽  
Splicing Factors ◽  
Rna Pol Ii ◽  
Pol Ii ◽  
General Transcription Factors ◽  
Sequential Entry ◽  
Processing Factors

In eukaryotic cells, RNA polymerase II (RNA pol II) transcription and pre-mRNA processing are coordinated events. We have addressed how individual components of the transcription and pre-mRNA processing machinery are organized during mitosis and subsequently recruited into the newly formed daughter nuclei. Interestingly, localization studies of numerous RNA pol II transcription and pre-mRNA processing factors revealed a nonrandom and sequential entry of these factors into daughter nuclei after nuclear envelope/lamina formation. The initiation competent form of RNA pol II and general transcription factors appeared in the daughter nuclei simultaneously, but prior to pre-mRNA processing factors, whereas the elongation competent form of RNA pol II was detected even later. The differential entry of these factors rules out the possibility that they are transported as a unitary complex. Telophase nuclei were competent for transcription and pre-mRNA splicing concomitant with the initial entry of the respective factors. In addition, our results revealed a low turnover rate of transcription and pre-mRNA splicing factors during mitosis. We provide evidence to support a model in which the entry of the RNA pol II gene expression machinery into newly forming daughter nuclei is a staged and ordered process.

scholarly journals Alternative splicing regulation of cell cycle genes by SPF45/SR140/CHERP complex controls cell proliferation

RNA ◽  
2021 ◽  
pp. rna.078935.121
Author(s):  
Elena Martin ◽  
Claudia Vivori ◽  
Malgorzata Rogalska ◽  
Jorge Herrero ◽  
Juan Valcarcel
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Alternative Splicing ◽  
Cell Cycle Progression ◽  
Molecular Mechanisms ◽  
Splicing Factors ◽  
Splicing Regulation ◽  
Cancer Cell Proliferation ◽  
Cycle Progression ◽  
Regulation Of Cell Cycle

The regulation of pre-mRNA processing has important consequences for cell division and the control of cancer cell proliferation but the underlying molecular mechanisms remain poorly understood. We report that three splicing factors, SPF45, SR140 and CHERP form a tight physical and functionally coherent complex that regulates a variety of alternative splicing events, frequently by repressing short exons flanked by suboptimal 3' splice sites. These comprise alternative exons embedded in genes with important functions in cell cycle progression, including the G2/M key regulator FOXM1 and the spindle regulator SPDL1. Knockdown of either of the three factors leads to G2/M arrest and to enhanced apoptosis in HeLa cells. Promoting the changes in FOXM1 or SPDL1 splicing induced by SPF45/SR140/CHERP knockdown partially recapitulate the effects on cell growth, arguing that the complex orchestrates a program of alternative splicing necessary for efficient cell proliferation.

Mutations in the large subunit of U2AF disrupt pre-mRNA splicing, cell cycle progression and nuclear structure

Yeast ◽  
2000 ◽  
Vol 16 (11) ◽  
pp. 1001-1013 ◽  
Author(s):  
Mitchell Beales ◽  
Nina Flay ◽  
Ron McKinney ◽  
Yasuaki Habara ◽  
Yasumi Ohshima ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Nuclear Structure ◽  
Cell Cycle Progression ◽  
Large Subunit ◽  
Mrna Splicing ◽  
Cycle Progression

scholarly journals DLP, a Novel Dim1 Family Protein Implicated in Pre-mRNA Splicing and Cell Cycle Progression

2004 ◽  
Vol 279 (31) ◽  
pp. 32839-32847 ◽  
Author(s):  
Xiaojing Sun ◽  
Hua Zhang ◽  
Dan Wang ◽  
Dalong Ma ◽  
Yan Shen ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Mrna Splicing ◽  
Cycle Progression ◽  
Family Protein

scholarly journals The Fission Yeast Pre-mRNA-processing Factor 18 (prp18+) Has Intron-specific Splicing Functions with Links to G1-S Cell Cycle Progression

2016 ◽  
Vol 291 (53) ◽  
pp. 27387-27402 ◽  
Author(s):  
Nagampalli Vijaykrishna ◽  
Geetha Melangath ◽  
Rakesh Kumar ◽  
Piyush Khandelia ◽  
Pushpinder Bawa ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Fission Yeast ◽  
Cell Cycle Progression ◽  
Mrna Processing ◽  
Processing Factor ◽  
Cycle Progression

scholarly journals SRSF1 regulates the assembly of pre-mRNA processing factors in nuclear speckles

2012 ◽  
Vol 23 (18) ◽  
pp. 3694-3706 ◽  
Author(s):  
Vidisha Tripathi ◽  
David Y. Song ◽  
Xinying Zong ◽  
Sergey P. Shevtsov ◽  
Stephen Hearn ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Polymerase Ii ◽  
Cell Nucleus ◽  
Interphase Nucleus ◽  
Sr Proteins ◽  
Mrna Processing ◽  
Splicing Factors ◽  
Nuclear Speckles ◽  
Nuclear Speckle ◽  
Processing Factors

The mammalian cell nucleus is compartmentalized into nonmembranous subnuclear domains that regulate key nuclear functions. Nuclear speckles are subnuclear domains that contain pre-mRNA processing factors and noncoding RNAs. Many of the nuclear speckle constituents work in concert to coordinate multiple steps of gene expression, including transcription, pre-mRNA processing and mRNA transport. The mechanism that regulates the formation and maintenance of nuclear speckles in the interphase nucleus is poorly understood. In the present study, we provide evidence for the involvement of nuclear speckle resident proteins and RNA components in the organization of nuclear speckles. SR-family splicing factors and their binding partner, long noncoding metastasis-associated lung adenocarcinoma transcript 1 RNA, can nucleate the assembly of nuclear speckles in the interphase nucleus. Depletion of SRSF1 in human cells compromises the association of splicing factors to nuclear speckles and influences the levels and activity of other SR proteins. Furthermore, on a stably integrated reporter gene locus, we demonstrate the role of SRSF1 in RNA polymerase II–mediated transcription. Our results suggest that SR proteins mediate the assembly of nuclear speckles and regulate gene expression by influencing both transcriptional and posttranscriptional activities within the cell nucleus.

scholarly journals Cohesin interacts with a panoply of splicing factors required for cell cycle progression and genomic organization

2018 ◽  
Author(s):  
Jung-Sik Kim ◽  
Xiaoyuan He ◽  
Jie Liu ◽  
Zhijun Duan ◽  
Taeyeon Kim ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Binding Proteins ◽  
Rna Binding ◽  
Genomic Organization ◽  
Rna Binding Proteins ◽  
Control Cell ◽  
Splicing Factors ◽  
Cycle Progression ◽  
Chromatid Cohesion

AbstractThe cohesin complex regulates sister chromatid cohesion, chromosome organization, gene expression, and DNA repair. Here we report that endogenous human cohesin interacts with a panoply of splicing factors and RNA binding proteins, including diverse components of the U4/U6.U5 tri-snRNP complex and several splicing factors that are commonly mutated in cancer. The interactions are enhanced during mitosis, and the interacting splicing factors and RNA binding proteins follow the cohesin cycle and prophase pathway of regulated interactions with chromatin. Depletion of cohesin-interacting splicing factors results in stereotyped cell cycle arrests and alterations in genomic organization. These data support the hypothesis that splicing factors and RNA binding proteins control cell cycle progression and genomic organization via regulated interactions with cohesin and chromatin.One Sentence SummaryEndogenous tagging reveals that cohesin interacts with diverse chromatin-bound splicing factors that regulate cell cycle progression and genomic organization in human cells.

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