Commentary on: Noninvasive Induction of Muscle Fiber Hypertrophy and Hyperplasia: Effects of High-Intensity Focused Electromagnetic Field Evaluated in an In-Vivo Porcine Model: A Pilot Study

2020 ◽  
Vol 40 (5) ◽  
pp. 575-576
Author(s):  
Jonathan Cook ◽  
Jason N Pozner
Keyword(s):  
Electromagnetic Field ◽  
Pilot Study ◽  
Muscle Fiber ◽  
High Intensity ◽  
Porcine Model

scholarly journals Electrotransfer of siRNA to Silence Enhanced Green Fluorescent Protein in Tumor Mediated by a High Intensity Pulsed Electromagnetic Field

Vaccines ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 49 ◽  
Author(s):  
Simona Kranjc Brezar ◽  
Matej Kranjc ◽  
Maja Čemažar ◽  
Simon Buček ◽  
Gregor Serša ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Green Fluorescent Protein ◽  
High Intensity ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Gene Electrotransfer ◽  
Pulsed Electromagnetic Field ◽  
Pulsed Electromagnetic ◽  
Green Fluorescent

The contactless high intensity pulsed electromagnetic field (HI-PEMF)-induced increase of cell membrane permeability is similar to conventional electroporation, with the important difference of inducing an electric field non-invasively by exposing a treated tissue to a time-varying magnetic field. Due to the limited number of studies in the field of electroporation induced by HI-PEMF, we designed experiments to explore the feasibility of such a contactless delivery technique for the gene electrotransfer of nucleic acids in tissues in vivo. By using HI-PEMF for gene electrotransfer, we silenced enhanced green fluorescent protein (EGFP) with siRNA molecules against EGFP in B16F10-EGFP tumors. Six days after the transfer, the fluorescent tumor area decreased by up to 39% as determined by fluorescence imaging in vivo. In addition, the silencing of EGFP to the same extent was confirmed at the mRNA and protein level. The results obtained in the in vivo mouse model demonstrate the potential use of HI-PEMF-induced cell permeabilization for gene therapy and DNA vaccination. Further studies are thus warranted to improve the equipment, optimize the protocols for gene transfer and the HI-PEMF parameters, and demonstrate the effects of HI-PEMF on a broader range of different normal and tumor tissues.

scholarly journals Noninvasive Induction of Muscle Fiber Hypertrophy and Hyperplasia: Effects of High-Intensity Focused Electromagnetic Field Evaluated in an In-Vivo Porcine Model: A Pilot Study

2019 ◽  
Vol 40 (5) ◽  
pp. 568-574 ◽  
Author(s):  
Diane Duncan ◽  
Ivan Dinev
Keyword(s):  
Muscle Tissue ◽  
Muscle Fiber ◽  
High Intensity ◽  
Structural Changes ◽  
Mass Density ◽  
Muscle Growth ◽  
Muscle Thickness ◽  
Fiber Density ◽  
Biceps Femoris Muscle

Abstract Background High-intensity focused electromagnetic (HIFEM) field technology has been reported to increase muscle thickness and hypertrophy. However, this process has not yet been confirmed on a histologic level. Objectives The aim of this study was to evaluate in-vivo structural changes in striated porcine muscle tissue following HIFEM treatment. Methods Three Yorkshire pigs received four 30-minute HIFEM treatments applied to the biceps femoris muscle on 1 side only. The fourth pig served as a control subject. At baseline and 2 weeks after the last treatment, biopsy specimens of the muscle tissue were collected from the treatment site. The control pig underwent muscle biopsy from a similar but untreated site. Twenty-five histology slides were evaluated from each pig. A certified histopathologist analyzed sliced biopsy samples for structural changes in the tissue. Results Histologic analysis showed hypertrophic changes 2 weeks posttreatment. The muscle mass density increased by 20.56% (to a mean of 17,053.4 [5617.9] µm2) compared with baseline. Similarly, muscle fiber density (hyperplasia) increased: the average change in the number of fibers in a slice area of 136,533.3 µm2 was +8.0%. The mean size of an individual muscle fiber increased by 12.15% (to 332.23 [280.2] µm2) 2 weeks posttreatment. Control samples did not show any significant change in fiber density or hyperplasia. Conclusions Histopathologic quantification showed significant structural muscle changes through a combination of fiber hypertrophy and hyperplasia. Control biopsies showed a lack of similar changes. The data correlate with findings of other HIFEM research and suggest that HIFEM could be used for noninvasive induction of muscle growth.

scholarly journals Intrabronchial Application of Extracellular Histones Shows No Proinflammatory Effects in Swine in a Translational Pilot Study

2021 ◽  
Author(s):  
Robert Ruemmler ◽  
Alexander Ziebart ◽  
Elisabeth Britten ◽  
Moritz Gosling ◽  
Rene Rissel ◽  
...  
Keyword(s):  
Pilot Study ◽  
Porcine Model ◽  
Inflammatory Responses ◽  
Mortality Rates ◽  
Injury Model ◽  
Molecular Factor ◽  
Extracellular Histones ◽  
Lung Injury Model ◽  
In Vivo Effects

Abstract Objective: Extracellular histones have been identified as one molecular factor that can cause and sustain alveolar damage and were linked to high mortality rates in critically ill patients. In this pilot study, we wanted to validate the proinflammatory in vivo effects of local and systemic histone application in a prospective translational porcine model. This was combined with the evaluation of an experimental acute lung injury model using intrabronchial lipopolysaccharides, which has been published previously.Results: The targeted application of histones was successful in all animals. Animals showed decreased oxygenation after instillation, but no differences could be detected between the sham and histone treatments. Additionally, intravenous histone injection had no effect. The histologic analyses and inflammatory responses indicated that there were no differences in tissue damage between the groups.

scholarly journals Endoscopic high-intensity focused US: technical aspects and studies in an in vivo porcine model (with video)

2015 ◽  
Vol 81 (5) ◽  
pp. 1243-1250 ◽  
Author(s):  
Tong Li ◽  
Tatiana Khokhlova ◽  
Ezekiel Maloney ◽  
Yak-Nam Wang ◽  
Samantha D'Andrea ◽  
...  
Keyword(s):  
High Intensity ◽  
Porcine Model ◽  
Technical Aspects

scholarly journals Pulsed-high intensity focused ultrasound enhanced tPA mediated thrombolysis in a novel in vivo clot model, a pilot study

2007 ◽  
Vol 121 (2) ◽  
pp. 193-202 ◽  
Author(s):  
Michael J. Stone ◽  
Victor Frenkel ◽  
Sergio Dromi ◽  
Peter Thomas ◽  
Ryan P. Lewis ◽  
...  
Keyword(s):  
Pilot Study ◽  
High Intensity ◽  
Focused Ultrasound ◽  
High Intensity Focused Ultrasound

scholarly journals Intrabronchial application of extracellular histones shows no proinflammatory effects in swine in a translational pilot study

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Robert Ruemmler ◽  
Alexander Ziebart ◽  
Elisabeth Britten ◽  
Moritz Gosling ◽  
Rene Rissel ◽  
...  
Keyword(s):  
Pilot Study ◽  
Porcine Model ◽  
Inflammatory Responses ◽  
Mortality Rates ◽  
Injury Model ◽  
Molecular Factor ◽  
Extracellular Histones ◽  
Lung Injury Model ◽  
In Vivo Effects

Abstract Objective Extracellular histones have been identified as one molecular factor that can cause and sustain alveolar damage and were linked to high mortality rates in critically ill patients. In this pilot study, we wanted to validate the proinflammatory in vivo effects of local histone application in a prospective translational porcine model. This was combined with the evaluation of an experimental acute lung injury model using intrabronchial lipopolysaccharides, which has been published previously. Results The targeted application of histones was successful in all animals. Animals showed decreased oxygenation after instillation, but no differences could be detected between the sham and histone treatments. The histologic analyses and inflammatory responses indicated that there were no differences in tissue damage between the groups.

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