A Novel and Rapid Method for the Preparation of Methyl Esters for Gas Chromatography: Application to the Determination of the Fatty Acids of Edible Fats and Oils

1978 ◽  
Vol 16 (8) ◽  
pp. 329-331 ◽  
Author(s):  
D. K. McCreary ◽  
W. C. Kossa ◽  
S. Ramachandran ◽  
R. R. Kurtz
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Rapid Method ◽  
Methyl Esters ◽  
Fats And Oils ◽  
Edible Fats

Comparison of Two Infrared Methods for the Determination of Isolated Trans Unsaturation in Fats, Oils, and Methyl Ester Derivatives

1971 ◽  
Vol 54 (6) ◽  
pp. 1288-1292
Author(s):  
Anita Huang ◽  
David Firestone
Keyword(s):  
Fatty Acids ◽  
Methyl Ester ◽  
Rapid Method ◽  
Unsaturated Fatty Acids ◽  
Methyl Esters ◽  
Tentative Method ◽  
Trans Isomers ◽  
Sample Matrix ◽  
Infrared Methods

Abstract A study was made to compare the rapid method reported by Allen with the tentative method of the American Oil Chemists’ Society for the determination of isolated (unconjugated) trans isomers of unsaturated fatty acids. The rapid method was found to be less accurate. The accuracy of the rapid method can be improved by using an oil or methyl ester matrix with the same composition as the sample matrix for the determination of K-and f-values used for calculation of per cent trans isomers. Results obtained with both methods for methyl esters were more accurate than results for vegetable oils. An analysis of variance was performed to compare the methods.

Determination of cis,cis-Methylene Interrupted Polyunsaturated Fatty Acids in Fats and Oils By Capillary Gas Chromatography

1986 ◽  
Vol 69 (1) ◽  
pp. 65-67
Author(s):  
Albert K Athnasios ◽  
Edward J Healy ◽  
Anthony F Gross ◽  
Gareth J Templeman
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Fatty Acid Methyl Esters ◽  
Capillary Column ◽  
Methyl Esters ◽  
Fats And Oils ◽  
Pufa Content ◽  
Acid Methyl ◽  
Relative Standard

Abstract A capillary gas chromatographic (CGC) method is described for the determination of cis,cis-methylene interrupted polyunsaturated fatty acids (cis-PUFA) in fats and oils. The sample is saponified and the liberated fatty acids are esterified to the corresponding methyl esters. The latter are analyzed by CGC using a 60M SP2340 capillary column. Area percent values for 9,12-cis,cis-C18:2 and 9,12,15-cis,cis,cis-C18:3 fatty acid methyl esters are summed to give the total cis-PUFA content. Gas chromatographic results agreed well with those obtained by an enzymatic lipoxygenase method at the 31-48% cis-PUFA levels with a correlation coefficient of 0.98. The method has a precision (relative standard deviation) of 0.33% at a 44.4% cis-PUFA level in margarine oil.

scholarly journals Comparison of Two Derivatization Methods for the Analysis of Fatty Acids andTransFatty Acids in Bakery Products Using Gas Chromatography

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Jumat Salimon ◽  
Talal A. Omar ◽  
Nadia Salih
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Methyl Esters ◽  
Correlation Coefficients ◽  
Bakery Products ◽  
Trimethylsilyl Diazomethane ◽  
Acid Catalyzed ◽  
High Level ◽  
Level Of Agreement

Two different procedures for the methylation of fatty acids (FAs) andtransfatty acids (TFAs) in food fats were compared using gas chromatography (GC-FID). The base-catalyzed followed by an acid-catalyzed method (KOCH3/HCl) and the base-catalyzed followed by (trimethylsilyl)diazomethane (TMS–DM) method were used to prepare FA methyl esters (FAMEs) from lipids extracted from food products. In general, both methods were suitable for the determination ofcis/transFAs. The correlation coefficients (r) between the methods were relatively small (ranging from 0.86 to 0.99) and had a high level of agreement for the most abundant FAs. The significant differences (P=0.05) can be observed for unsaturated FAs (UFAs), specifically for TFAs. The results from the KOCH3/HCl method showed the lowest recovery values (%R) and higher variation (from 84% to 112%), especially for UFAs. The TMS-DM method had higherRvalues, less variation (from 90% to 106%), and more balance between variation and %RSD values in intraday and interday measurements (less than 4% and 6%, resp.) than the KOCH3/HCl method, except for C12:0, C14:0, and C18:0. Nevertheless, the KOCH3/HCl method required shorter time and was less expensive than the TMS-DM method which is more convenient for an accurate and thorough analysis of richcis/transUFA samples.

Direct ultrasound-assisted methylation of fatty acids in serum for free fatty acid determinations

2010 ◽  
Vol 88 (9) ◽  
pp. 898-905 ◽  
Author(s):  
Liyan Liu ◽  
Ying Li ◽  
Rennan Feng ◽  
Changhao Sun
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Ultrasonic Waves ◽  
Acid Methyl ◽  
Ultrasound Assisted

A method for simultaneous determination of 16 free fatty acids (FFAs) in serum is described. The method involves conversion of FFAs to fatty acid methyl esters (FAMEs) using the heat of ultrasonic waves followed by gas chromatography and mass spectrometry (GC–MS) analysis. Optimum levels of the variables affecting the yield of FAMEs were investigated. The results indicate that the optimal levels are 55 °C, 60 W, 10% H2SO4/CH3OH, and 50 min. Recoveries ranged from 85.32% to 112.11%, with a detection limit ranging from 0.03 to 0.08 μg mL–1. The linearity, using the linear correlation coefficient, was higher than 0.9914.

scholarly journals DETERMINATION OF FATTY ACIDS IN SOLANUM SURATTENSE BURM. F. BY USING GAS CHROMATOGRAPHY

2017 ◽  
Vol 10 (8) ◽  
pp. 60 ◽  
Author(s):  
Raman Preet ◽  
Raghbir Chand Gupta
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Methyl Esters ◽  
Basic Research ◽  
Fatty Acid Analysis ◽  
Operating Conditions ◽  
Solanum Surattense ◽  
Flame Ionization

  Objective: This study aims to document the fatty acid composition of Solanum surattense Burm. f. collected from hot desert of India, Rajasthan.Methods: The fatty acid analysis was performed by gas chromatography-flame ionization detector (GC-FID). The operating conditions used to examine methyl esters of fatty acids are as follows. Fatty acids were converted into methyl esters (FAMEs) before GC analysis according to the standard methods by Ranganna (1986). Quantitative determinations of FAMEs were conducted using GC-FID and capillary column HP-88 Agilent Technologies.Results: The most abundant fatty was palmitic acid (13.2%), oleic acid (22.9%), and linoleic acid (11.9%). This plant is good source of important fatty acids including all the groups of saturated, monounsaturated, and polyunsaturated fatty acids (MUFAs and PUFAs) and can be used as a commercial source of fatty acids especially MUFAs and PUFAs.Conclusion: The plant is well studied for various pharmacological activities such as antiasthmatic, anticancer, cardiovascular, and hepatoprotective. Determination of fatty acid profiles in nutritional and clinical research with precision and fastness has become popular for human health and basic research.

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