Separation of 9-Methoxycamptothecin and Camptothecin from Nothapodytes foetida by Semipreparative HPLC

In vitro seed germination and embryo culture have been achieved in Nothapodytes foetida, this plant is known for its rich source of anticancer drug i. e., Camptothecin. In present study both normal and decoated seeds were subjected to different treatments viz., H2O, GA3, H2O2, H2SO4, chlorine water and mechanical scarification, further these were germinated on water agar medium (WA), filter paper bridge (FB), half strength MS (HMS) and full strength MS (FMS) medium. The highest percentage (69%) of germination was achieved from decoated seeds treated with 10mg/L GA3 and germinated on Filter Paper Bridge. And for embryo culture mature embryos were inoculated on MS medium containing various combination and concentrations of cytokinins (BAP, Kn and TDZ) and auxin (IAA and NAA) for rapid conversion into a plantlet. Among the different combinations of growth regulators; highest frequency (100%) of plantlet conversion was obtained on MS medium containing Kn (1.0mg/L) and NAA (0.2mg/L).

Download Full-text

Structural and photophysical characterisation of coordination and optical isomers of mononuclear ruthenium(ii) polypyridyl 1,2,4-triazole complexesElectronic supplementary information (ESI) available: analytical and semipreparative HPLC chromatograms, CD and UV/vis spectra. See http://www.rsc.org/suppdata/dt/b3/b301961f/

Dalton Transactions ◽

10.1039/b301961f ◽

2003 ◽

pp. 2597 ◽

Cited By ~ 27

Author(s):

Wesley R. Browne ◽

Dusan Hesek ◽

John F. Gallagher ◽

Christine M. O'Connor ◽

J. Scott Killeen ◽

...

Keyword(s):

Supplementary Information ◽

Optical Isomers ◽

Semipreparative Hplc

Download Full-text

9-Methoxycamptothecin from Nothapodytes foetida Induces Apoptosis in Murine Sarcoma S180 Cells

Zeitschrift für Naturforschung C ◽

10.1515/znc-2011-9-1006 ◽

2011 ◽

Vol 66 (9-10) ◽

pp. 471-476

Author(s):

Na Liao ◽

Peng Zhang ◽

Mingzhang Ao ◽

Jing Wang ◽

Yueyuan Shi ◽

...

Keyword(s):

Cell Death ◽

Nmr Spectroscopy ◽

Mtt Assay ◽

Propidium Iodide ◽

Annexin V ◽

Double Staining ◽

Nothapodytes Foetida ◽

Ic50 Value ◽

Mitochondria Pathway ◽

Murine Sarcoma

9-Methoxycamptothecin (MCPT) was found to have antitumour activities through topoisomerase inhibition. However, the type of cell death induced in the tumour cells treated with MCPT was not elucidated. In this study, MCPT and camptothecin were isolated from Nothapodytes foetida distributed in Hubei Province, China and identifi ed by NMR spectroscopy. MCPT was tested by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay using camptothecin as reference. Annexin V-FITC/propidium iodide double staining and real-time PCR were also performed. The IC50 value was (0.385 ± 0.08) μM. The apoptosis rates increased from 9.5% to 17.27%, 30.14%, and 66.46% with an increase in MCPT concentrations from 0, 0.19, 0.38, to 0.95 μM, respectively. The ratio of Bax/Bcl-2 also increased from 1 to 1.61, 2.43, and 4.57, respectively. Bax and Bcl-2 are crucial to the mitochondria pathway. The results indicate that the mitochondria pathway may be involed in MCPT-induced murine sarcoma S180 apoptosis.

Download Full-text