Serum Glutamic—Oxaloacetic Transaminase: Evaluation of a Coupled-Reaction Enzyme Assay by Means of Kinetic Theory

1971 ◽  
Vol 17 (11) ◽  
pp. 1114-1122 ◽  
Author(s):  
C D Russell ◽  
E Cotlove
Keyword(s):  
Kinetic Theory ◽  
Enzyme Assay ◽  
Rate Equations ◽  
Enzyme Assays ◽  
Glutamic Oxaloacetic Transaminase ◽  
Malic Dehydrogenase ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Coupled Reaction ◽  
Analytical Errors ◽  
Substrate Concentrations

Abstract Rate equations and rate parameters are determined for the coupled-reaction assay for serum glutamic-oxaloacetic transaminase in which malic dehydrogenase is used. These are used to interpret observed curves of absorbance vs. time, to select optimum substrate concentrations, and to estimate nonrandom analytical errors. A simple, systematic, general approach is presented, which can readily be applied to other enzyme assays in which two or more reactions are coupled. It depends for its simplicity on access to an adequate computing facility.

Application of response surface methodology to the assay of gamma-glutamyltransferase.

1982 ◽  
Vol 28 (5) ◽  
pp. 1140-1143 ◽  
Author(s):  
J W London ◽  
L M Shaw ◽  
L Theodorsen ◽  
J H Stromme
Keyword(s):  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Response Surface ◽  
Enzyme Assay ◽  
Enzyme Assays ◽  
Transferase Activity ◽  
Enzyme Kinetic ◽  
Gamma Glutamyltransferase ◽  
Measured Activity ◽  
Substrate Concentrations

Abstract Response surface methodology (RSM) offers an empirical approach to the study of clinical enzyme assays. Variables such as pH, which are difficult to characterize by using theoretical enzyme kinetics, are easily included in RSM formulations. In this investigation, we studied with RSM the change in the measured activity of gamma-glutamy-transferase (EC 2.3.2.2) as a function of changes in concentrations of donor (gamma-glutamyl-3-carboxy-4-nitroanilide) acceptor (glycylglycine), and pH. The study defined large ranges for these variables over which maximum enzyme activity is obtained: donor 6.6 to 10.2 mmol/L, acceptor 129 to 250 mmol/L, and pH 7.8 to 8.5. The RSM regression polynomial was as accurate as a previously determined enzyme kinetic equation for predicting the transferase activity from given reagent substrate concentrations. Although not yielding a mechanistic understanding of an enzyme assay, RSM studies do produce an operational understanding of how an assay functions.

scholarly journals Extracellular α-Galactosidase from Trichoderma sp. (WF-3): Optimization of Enzyme Production and Biochemical Characterization

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Aishwarya Singh Chauhan ◽  
Arunesh Kumar ◽  
Nikhat J. Siddiqi ◽  
B. Sharma
Keyword(s):  
Enzyme Activity ◽  
Enzyme Assay ◽  
Biochemical Characterization ◽  
Catalytic Efficiency ◽  
Culture Conditions ◽  
Significant Loss ◽  
Trichoderma Sp ◽  
Rate Of Reaction ◽  
Higher Temperature ◽  
Substrate Concentrations

Trichoderma spp. have been reported earlier for their excellent capacity of secreting extracellular α-galactosidase. This communication focuses on the optimization of culture conditions for optimal production of enzyme and its characterization. The evaluation of the effects of different enzyme assay parameters such as stability, pH, temperature, substrate concentrations, and incubation time on enzyme activity has been made. The most suitable buffer for enzyme assay was found to be citrate phosphate buffer (50 mM, pH 6.0) for optimal enzyme activity. This enzyme was fairly stable at higher temperature as it exhibited 72% activity at 60°C. The enzyme when incubated at room temperature up to two hours did not show any significant loss in activity. It followed Michaelis-Menten curve and showed direct relationship with varying substrate concentrations. Higher substrate concentration was not inhibitory to enzyme activity. The apparent Michaelis-Menten constant (Km), maximum rate of reaction (Vmax), Kcat, and catalytic efficiency values for this enzyme were calculated from the Lineweaver-Burk double reciprocal plot and were found to be 0.5 mM, 10 mM/s, 1.30 U mg−1, and 2.33 U mg−1 mM−1, respectively. This information would be helpful in understanding the biophysical and biochemical characteristics of extracellular α-galactosidase from other microbial sources.

scholarly journals Clinical and therapeutic correlations in patients with slight acute pancreatitis

2015 ◽  
Vol 28 (1) ◽  
pp. 24-27 ◽  
Author(s):  
Clewis Henri MUNHOZ-FILHO ◽  
Fernando BATIGÁLIA ◽  
Hamilton Luiz Xavier FUNES
Keyword(s):  
Acute Pancreatitis ◽  
Intensive Care ◽  
Vital Signs ◽  
Positive Association ◽  
Multiple Organ ◽  
Glutamic Oxaloacetic Transaminase ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Early Return ◽  
Lower Spectrum ◽  
Mild Acute Pancreatitis

BACKGROUND: Acute pancreatitis is an inflammatory disease of the pancreas due to enzymatic autodigestion which can cause necrosis or multiple organ failure; its pathophysiology is not fully known yet. AIM: To evaluate the correlation between clinical and therapeutic data in patients with mild acute pancreatitis. METHODS: A retrospective study in 55 medical records of patients admitted with acute mild pancreatitis was realized to analyze the association between age, leukocytosis, serum glutamic-oxaloacetic transaminase and lactate dehydrogenase, glucose, antibiotics, time admission and Ranson´s scores. RESULTS: There was a positive association between less intensive care (strict hydration, analgesia and monitoring of vital signs), early antibiotic therapy (monotherapy), early return to diet after 48 hours and laboratory control of the serum amylase and lipase (high in the first week and decreasing after 10 days, without any prognostic value). CONCLUSIONS: Changes in the management of patients with mild acute pancreatitis, such as enteral nutrition, rational use of lower spectrum antibiotics and intensive care, have contributed significantly to the reduction of hospitalization time and mortality.

scholarly journals PENGARUH AKTIFITAS FISIK CONTINUOUS RUNNING DAN INTERVAL RUNNING TERHADAP SERUM GLUTAMIC OXALOACETIC TRANSAMINASE (SGOT) DAN SERUM GLUTAMIC PYRUVIC TRANSAMINASE (SGPT)

2019 ◽  
Vol 3 (1) ◽  
pp. 12
Author(s):  
Novita Sari Harahap ◽  
Riski Pranata
Keyword(s):  
Glutamic Pyruvic Transaminase ◽  
Glutamic Oxaloacetic Transaminase ◽  
Serum Glutamic Pyruvic Transaminase ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Group Design ◽  
Continuous Running

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh aktifitas fisik continuous running dan interval running terhadap SGOT dan SGPT pada mahasiswa Ikor Unimed Tahun 2017. Penelitian dilakukan di Stadion Universitas Negeri Medan, Laboratorium Fisik FIK, dan Laboratorium Kesehatan Daerah Dinas Kesehatan Provinsi Sumatera Utara. Metode penelitian yang digunakan dalam penelitian ini adalah metode eksperimen. Sampel penelitian menggunakan mahasiswa Ikor Unimed  sebanyak 14 orang. Desain penelitian menggunakan Pretest-Posttest Group Design. Aktifitas fisik continuous Running dan interval running dilakukan dengan menggunakan Treadmill selama 12 menit dengan intensitas 60-70 %. Pemeriksaan kadar SGOT dan SGPT dilakukan di awal dan di akhir perlakuan. Teknik analisis data pada penelitian ini menggunakan uji t.  Hasil penelitian menunjukkan  bahwa peningkatan rerata kadar SGOT setelah aktifitas fisik continuous running sebesar 88,00 U/L yang sebelumnya sebesar 60,89 U/L dan setelah aktifitas fisik interval running sebesar 81,61 U/L yang sebelumnya 60,41 U/L. Hasil uji analisis diperoleh nilai p=0,662, yang menunnjukkan bahwa ada peningkatan namun tidak terdapat perbedaan yang bermakna p>0,05. Begitu juga dengan rerata kadar SGPT setelah aktifitas fisik continuous running sebesar 82,21 U/L yang sebelumnya sebesar 57,16 U/L dan setelah aktifitas fisik interval running sebesar 81,80 U/L yang sebelumnya 59,59 U/L. Hasil uji analisis diperoleh nilai p=0,655, yang menunnjukkan bahwa ada peningkatan namun tidak terdapat perbedaan yang bermakna p>0,05. Penelitian ini menyimpulkan bahwa bahwa tidak terdapat perbedaan yang bermakna antara aktifitas fisik continuous running dan aktifitas fisik interval running terhadap peningkatan SGOT dan SGPT pada mahasiswa IKOR UNIMED tahun 2017.Kata kunci: Continuous Running, Interval Running, SGOT dan SGPT

scholarly journals Effect of Cardiac Catheterization and Angiocardiography on the Serum Glutamic Oxaloacetic Transaminase

Circulation ◽  
1963 ◽  
Vol 27 (4) ◽  
pp. 565-570 ◽  
Author(s):  
ZAVEN A. ADROUNY ◽  
MAX J. STEPHENSON ◽  
KURT R. STRAUBE ◽  
CHARLES T. DOTTER ◽  
HERBERT E. GRISWOLD
Keyword(s):  
Cardiac Catheterization ◽  
Glutamic Oxaloacetic Transaminase ◽  
Serum Glutamic Oxaloacetic Transaminase

Automated Determination of Serum Glutamic Oxaloacetic Transaminase

1967 ◽  
Vol 13 (4) ◽  
pp. 270-275 ◽  
Author(s):  
Stanley Morgenstern ◽  
James H Kaufman ◽  
Bernard Klein
Keyword(s):  
Diazonium Salt ◽  
Glutamic Oxaloacetic Transaminase ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Oxaloacetic Acid ◽  
Automated Determination

Abstract A new and sensitive procedure is described for the automated determination of serum glutamic oxaloacetic transaminase using the Robot Chemist. Enzymically generated oxaloacetic acid is directly coupled with the diazonium salt, fast ponceau L, to form a colored solution whose absorbance is measured at 455 mµ. Correlations are presented between this method and the AutoAnalyzer procedure using the same reagent.

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