scholarly journals The Study of Ranitidine Interference With Morphine Detection Test by Thin-Layer Chromatography

2018 ◽  
Vol 6 (1) ◽  
pp. 21-23
Author(s):  
Amir Miri ◽  
Amir Karami ◽  
Fourogh Nadi ◽  
Fatemeh Zeraati
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Drugs Of Abuse ◽  
False Negative ◽  
Urine Samples ◽  
Urine Drug Screen ◽  
Test Results ◽  
Two Samples ◽  
Before And After ◽  
Layer Chromatography

Background: Drug abuse is a global and critical problem. One of the most frequent practices done in order to detect the drugs of abuse is Urine Drug Screen. However, for changing the drug test results, adulterants and urine substitutes are being designed. As the referring people’s background has shown, ranitidine is one of the interfering drugs in morphine detection test. Therefore, in the present study, the interference of ranitidine in morphine detection test will be studied. Methods: Ten healthy volunteers who had not used any kind of drug for 72 hours before the test were recruited into the study. First, 2 doses of ranitidine (150 and 300 mg) were administered to the subjects orally and 100-mL urine samples were collected from them before and after taking ranitidine. The second urine sample was collected at 6-8 am. Ten micrograms morphine was added to both urine samples of each individual. The urine samples were tested using thin-layer chromatography (TLC) technique. The experiment was repeated after 1 week using ranitidine 300 mg. Results: The TLC test was carried out on 40 urine samples. Twenty samples were tested before and, the rest, after ranitidine consumption. The TLC test results were positive before ranitidine consumption but negative for 18 samples and positive for two samples after taking ranitidine. Conclusion: Ranitidine may change the urine morphine screening test results via TLC method and induce a false negative result.

An Evaluation of the Measurement of Drugs of Abuse by Commercial and In-House Horizontal Thin Layer Chromatography

1993 ◽  
Vol 30 (2) ◽  
pp. 163-168 ◽  
Author(s):  
K Wolff ◽  
M J Sanderson ◽  
A W M Hay ◽  
I Barnes
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Drug Addiction ◽  
Drugs Of Abuse ◽  
Routine Screening ◽  
Suitable Method ◽  
Screening Service ◽  
Laboratory Methods ◽  
Opioid Drugs ◽  
Layer Chromatography

We have compared an in-house horizontal thin layer chromatography (TLC) method with a commercial TLC screening kit (Toxi-Lab) to find the most suitable method for screening urine for opioid drugs. The in-house TLC procedure is cheaper and more efficient than the commercial TLC method and is ideal as a confirmatory method when used in conjunction with other established laboratory methods. The in-house TLC method is useful for one-off requests and is able to differentiate between small quantities (0·1–0·5 mg/L) of the different, commonly abused opioids and their metabolites, which means that it is well suited for use at a local Drug Addiction Unit as a routine screening service.

Single-pH Extraction Procedure for Detecting Drugs of Abuse

1974 ◽  
Vol 20 (2) ◽  
pp. 309-311 ◽  
Author(s):  
Ramon E Stoner ◽  
Connie Parker
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Drugs Of Abuse ◽  
Clinical Laboratory ◽  
Extraction Procedure ◽  
Organic Salt ◽  
Basic Drugs ◽  
Laboratory Service ◽  
The Individual ◽  
Layer Chromatography

Abstract A method is presented for determining certain drugs of abuse in a single extract of urine. The urine sample is adjusted to pH 6.0 with a buffer containing bromcresol purple. Basic drugs, such as amphetamines and narcotics, form an organic salt with the ionized bromcresol purple, which is extractable with a mixture of chloroform and 2-propanol (3:1 by volume). At pH 6.0, weak acids such as barbiturates and neutral drugs such as glutethimide are also soluble in this solvent. Consequently, the major classes of drugs are extracted simultaneously. The extract is then concentrated and the individual drugs are determined by thin-layer chromatography in a solvent that will separate bromcresol purple from the drugs. Additional Keyphrases: drug screening #{ 149} toxicology #{ 149} thin-layer chromatography #{ 149} screening procedure Clinical Laboratory Service, West Side VA Hosp., 820 S. Damen Ave., Chicago, Ill. 60612.

scholarly journals Factitious Diarrhea Induced by Stimulant Laxatives: Accuracy of Diagnosis by a Clinical Reference Laboratory Using Thin Layer Chromatography

2007 ◽  
Vol 53 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Joseph H Shelton ◽  
Carol A Santa Ana ◽  
Donald R Thompson ◽  
Michael Emmett ◽  
John S Fordtran
Keyword(s):  
United States ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Stool Specimen ◽  
False Negative ◽  
The United States ◽  
Positive Zero ◽  
Reference Laboratory ◽  
Clinical Value ◽  
Layer Chromatography

Abstract Background: Surreptitious ingestion of laxatives can lead to serious factitious diseases that are difficult to diagnose. Most cases involve ingestion of bisacodyl or senna. Thin layer chromatography (TLC) of urine or stool is the only commercially available test for these laxatives. Such testing is considered highly reliable, but its accuracy in clinical practice is unknown. Our aim was to evaluate the reliability of TLC laxative testing by a clinical reference laboratory in the United States. Methods: Diarrhea was induced in healthy volunteers by ingestion of bisacodyl, senna, or a control laxative (n = 11 for each laxative group). Samples of urine and diarrheal stool were sent in blinded fashion to the clinical reference laboratory for bisacodyl and senna analysis. Results: TLC testing for bisacodyl-induced diarrhea revealed a sensitivity of 73% and specificity of 91% when urine was tested and sensitivity and specificity of 91% and 96%, respectively, when stool was analyzed. When diarrhea was induced by senna, the TLC assay for senna failed to identify even a single urine or stool specimen as positive (zero% sensitivity). Conclusions: Considering the expected prevalence of surreptitious laxative abuse in patients with chronic idiopathic diarrhea (2.4%–25%, depending on the clinical setting), TLC of urine or stool for bisacodyl by this reference laboratory would often produce misleading results, and testing for senna would have no clinical value. The major problems are false-positive tests for bisacodyl and false-negative tests for senna.

Effects of60Co Radiation on Herbicides in Aqueous Solution

Weed Science ◽  
1977 ◽  
Vol 25 (3) ◽  
pp. 200-202
Author(s):  
D.L. Bucholtz ◽  
T.L. Lavy
Keyword(s):  
Aqueous Solution ◽  
Acid Solution ◽  
Gamma Radiation ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chromatographic Methods ◽  
Before And After ◽  
Anisic Acid ◽  
Layer Chromatography

Phytotoxicity of herbicides in water was measured before and after exposure to gamma radiation from a60Co irradiator. Chromatographic methods and bioassays were used to measure alterations that occurred during60Co exposure. Phytotoxicity of picloram (4-amino-3,5,6-trichloropicolinic acid and trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) solutions was reduced even with the lowest dose of60Co, 1.7-megarad (Mrad). Alterations in phytotoxicity of atrazine (2-chloro-4-ethylamino-6-esopropylamino-s-triazine) and dicamba (3,6-dichloro-o-anisic acid) solution occurred only at 5 Mrad, which was the highest dose used. The phytotoxicity of atrazine solutons increased with the 5-Mrad irradiation. Thin layer chromatography (TLC) data showed that all herbicides were altered by60Co irradiation. Several products resulted when trifluralin was irradiated.

Use of Fluorescamine ("Fluram") to Detect Amphetamine in Urine by Thin-Layer Chromatography

1974 ◽  
Vol 20 (2) ◽  
pp. 272-274 ◽  
Author(s):  
Bernard Klein ◽  
Joan E Sheehan ◽  
E Grunberg
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Drugs Of Abuse ◽  
Thin Layer Plate ◽  
Spray Reagent ◽  
Layer Chromatography

Abstract A "Fluram" (fluorescamine; 4-phenylspiro[furan-2(3H)-1'-phthalan]-3,3'-dione) spray reagent will detect as little as 250 ng of amphetamine in an extract of amphetamine-containing urine, after it has been separated by thin-layer chromatography. The fluorescence is stable for about 20 h and can be renewed by respraying. Fluram spray does not interfere with reagent sprays used to detect other drugs of abuse. Amphetamine on the thin-layer plate can be measured by extracting the fluorescent area of silica gel and measuring the fluorescence in a fluorimeter. Recovery of 1.0 µg and 2.0 µg of amphetamine averaged 69% and 67%, respectively.

Dual-bed thin-layer chromatography for the separation and detection of drugs of abuse in urine

1973 ◽  
Vol 80 (2) ◽  
pp. 275-278 ◽  
Author(s):  
J.R. Broich ◽  
S. Goldner ◽  
G. Gourdet ◽  
S. Andryauskas ◽  
C.J. Umberger ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Drugs Of Abuse ◽  
Layer Chromatography ◽  
Dual Bed

scholarly journals LUPEOL VALIDATION AND QUANTIFICATION IN HETEROPOGON CONTORTUS (L.) BEAUV. (SPEAR GRASS) THROUGH HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

2017 ◽  
Vol 10 (12) ◽  
pp. 392 ◽  
Author(s):  
Navjot Kaur ◽  
Raghbir Chand Gupta
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Marker Compound ◽  
Heteropogon Contortus ◽  
Methanolic Extracts ◽  
Validation And Quantification ◽  
Two Samples ◽  
Layer Chromatography

Objective: This is aimed to study the chromatographic evaluation of triterpenoid, i.e., lupeol from methanolic extracts of leaves, stem, and inflorescence of Heteropogon contortus.Methods: The high-performance thin-layer chromatography (HPTLC) densitometry determination of lupeol was performed using optimized mobile phase toluene:methanol:formic acid (7:3:0.3 v/v) with a derivatization of freshly prepared anisaldehyde-sulfuric acid. For densitometry measurements, the plates were scanned at 530 nm absorbance/reflectance wavelength. Quantification of lupeol marker compound in H. contortus leaves, stem, and inflorescence is estimated using 2-12 μg/spot.Results: The appearance of light purple bands on the chromatograms confirmed the lupeol component in plant samples. Further, the confirmation of the compound is done from the densitometric scanning by comparing λmax values. From this, it is reported that lupeol is present in leaf samples, i.e., 10 mg/g of dry wt., while in rest of the two samples, it is found absent.Conclusion: The leaves of H. contortus (spear grass) are a good source of lupeol and can be used as an alternate natural source to synthesize herbal drugs to control cancer and other anti-inflammatory agents. The presently selected HPTLC is validated and most accurate for the quantification and identification of lupeol present in the selected plant. The leaves of the species which are rich in lupeol can be used in pharmaceutical industry.

Radioimmunuassay compared to thin-layer and gas--liquid chromatography for detecting methadone in human urine.

1976 ◽  
Vol 22 (11) ◽  
pp. 1915-1918 ◽  
Author(s):  
D L Roerig ◽  
R I Wang ◽  
M M Mueller ◽  
D L Lewand ◽  
S M Adams
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Methadone Maintenance ◽  
Screening Method ◽  
Urine Samples ◽  
Gas Liquid Chromatography ◽  
The Difference ◽  
Liquid Chromatographic ◽  
Layer Chromatography ◽  
Positive Results

Abstract Radioimmunoassay was compared to thin-layer and gas--liquid chromatographic methods for detection of methadone in the urine of patients undergoing methadone maintenance therapy as treatment of heroin abuse. With urine samples known to contain methadone, 84% were positive by thin-layer chromatography as compared to 99% positives by the other two methods. This difference is attributed to the difference in sensitivity of the three methods. All three methods gave consistently positive results with urine samples from patients receiving 25 mg of methadone per day or more. With smaller daily doses the percentage of positive results obtained with thin-layer chromatography decreased. Analysis of urine samples not containing methadone showed no incidence of cross reaction of other drugs with the methadone radioimmunoassay. The methadone radioimmunoassay appears to be both sensitive and reliable; however, certain other factors limit its use as a primary screening method.

scholarly journals Selective Separation and Simultaneous Determination of Trace Levels of Five Types of Fluorinated Quinolone Drugs by Thin-Layer Chromatography/Fluorescence Densitometry

2001 ◽  
Vol 84 (3) ◽  
pp. 684-688 ◽  
Author(s):  
Pei-Lan Wang ◽  
Liang Chen ◽  
Ying-Fang Fan
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Simultaneous Determination ◽  
Analytical Method ◽  
Selective Separation ◽  
Urine Samples ◽  
Plasma And Urine Samples ◽  
Layer Chromatography ◽  
High Repeatability

Abstract This paper reports the determination of trace levels of 5 types of fluorinated quinolone drugs, i.e., ciprofloxacin, norfloxacin, enoxacin, pefloxacin, and ofloxacin, by thin-layer chromatography (TLC)/fluorescence densitometry. The new analytical method uses 2-step TLC development, selective separation, and simultaneous determination of the 5 drugs. The method was also applied to the determination of recoveries of standards of the 5 drugs in plasma and urine samples. The results show that the method has a wide linear range, high repeatability, and good stability.

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