Automated Immunochemical Method for Determination of Urinary Protein of Plasma Origin

1975 ◽  
Vol 21 (10) ◽  
pp. 1465-1468 ◽  
Author(s):  
Lawrence M Killingsworth ◽  
Carol E Britain ◽  
Linda L Woodard
Keyword(s):  
Polyethylene Glycol ◽  
Reaction Time ◽  
Human Plasma ◽  
Continuous Flow ◽  
Rapid Determination ◽  
Reaction Medium ◽  
Urinary Proteins ◽  
Immunochemical Method ◽  
Antigen Antibody

Abstract An automated continuous-flow procedure has been developed for the rapid determination of urinary proteins of plasma origin. Antiserum to whole human plasma was used as the reagent, and the antigen—antibody reactions were quantitated by nephelometry. By adding polyethylene glycol (mol wt 6000-7500) to the reaction medium, reaction time was decreased to <3 min; no sample blanks were required; and samples were analyzed at a rate of 70 per hour. Recovery studies yielded an average of 98.5% of the added protein. In-run replicate precision (CV) of the method was 1.45%; day-to-day precision was 2.58%.

Polymer Enhancement of Automated Immunological Nephelometric Analysis, as Illustrated by Determination of Urinary Albumin

1974 ◽  
Vol 20 (4) ◽  
pp. 415-420 ◽  
Author(s):  
Jorge Lizana ◽  
Kristoffer Hellsing
Keyword(s):  
Polyethylene Glycol ◽  
Reaction Time ◽  
Comparative Study ◽  
Continuous Flow ◽  
Flow System ◽  
Urinary Albumin ◽  
Immunological Reaction ◽  
Enhancing Effect ◽  
Highly Sensitive

Abstract We have developed a new automated immunonephelometric method for determination of albumin in urine, by exploiting the polymer-enhancing effect on the immunological reaction. The original continuous-flow manifold has been simplified and the reaction time shortened to about 3 min. The antiserum was diluted 100-fold in a solution (100 g/liter) of polyethylene glycol (av mol wt, 6000) and mixed with the prediluted sample in the continuous-flow system. The method is highly sensitive and is unaffected by high blank and (or) high absorbance values of the specimens. Day-to-day variation was 3.8-4.3% (CV). Accuracy, as estimated from recovery experiments, was also good. A comparative study of 176 urines with the single radial immunodiffusion technique showed a correlation coefficient of 0.994. We therefore suggest the new method for routine use for determination of urinary albumin, because it is fast, accurate, precise, and sensitive, and requires only small amounts of antiserum.

Rapid determination of fenoldopam in human plasma by UPLC–MS/MS for pharmacokinetic analysis in patients

2015 ◽  
Vol 978-979 ◽  
pp. 78-82 ◽  
Author(s):  
Xipei Wang ◽  
Zhijie Zheng ◽  
Guodong He ◽  
Liping Mai ◽  
Zhiling Zhou ◽  
...  
Keyword(s):  
Human Plasma ◽  
Rapid Determination ◽  
Pharmacokinetic Analysis

Immunochemical Determination of Serum Albumin with a Centrifugal Analyzer

1975 ◽  
Vol 21 (2) ◽  
pp. 195-198 ◽  
Author(s):  
Mogens Blom ◽  
Niels Hjørne
Keyword(s):  
Polyethylene Glycol ◽  
Reaction Time ◽  
Serum Albumin ◽  
Human Serum ◽  
Albumin Concentration ◽  
Good Precision ◽  
Standard Curve ◽  
Immunochemical Determination ◽  
Human Sera

Abstract The turbidity resulting from the reaction between albumin and specific anti-human serum was measured with a good precision by using a GEMSAEC centrifugal analyzer. The reaction was enhanced by polyethylene glycol to shorten the reaction time (5 min) and to displace the point of equivalence between antigen and antibody to an albumin concentration unlikely to occur in human sera (about 100 g/liter). An additional program for the computer was necessary to fit the absorbance readings of individual sera to the nonlinear standard curve. Serum albumin values obtained by the described method correlated well with values obtained by the electroimmuno-technique. About 100 samples could be analyzed per hour, 500 µl of 100-fold diluted antiserum being used per specimen.

Optimizing determination of plasma albumin by the bromcresol green dye-binding method.

1981 ◽  
Vol 27 (1) ◽  
pp. 144-146 ◽  
Author(s):  
W S Robertson
Keyword(s):  
Reaction Time ◽  
Linear Regression ◽  
Human Plasma ◽  
Protein Composition ◽  
Improved Method ◽  
Reaction Conditions ◽  
Plasma Albumin ◽  
Optimal Reaction ◽  
Bromcresol Green

Abstract Some modifications of the conditions of the reaction between plasma and bromcresol green have led to an improved method for determination of plasma albumin with the Vickers M300 multichannel analyzer. Dye concentration and reaction time are the factors principally influencing method specificity, but variable protein composition of human plasma also affects it, so that optimal reaction conditions vary from specimen to specimen. Thus a compromise must be reached such that the best conditions for determining plasma albumin over a range of different protein concentrations are achieved. In the proposed method for the Vickers M300 a reaction time of 12 s (the minimum possible) is used. Comparison with "rocket" immunoelectrophoresis gave the following linear regression: y = 10 + 0.79 x (n = 91; r = 0.96).

A rate nephelometer for measuring specific proteins by immunoprecipitin reactions.

1977 ◽  
Vol 23 (8) ◽  
pp. 1456-1464 ◽  
Author(s):  
J C Sternberg
Keyword(s):  
Maximum Rate ◽  
Serum Proteins ◽  
Rapid Determination ◽  
Scattered Light ◽  
Single Point ◽  
Rate Of Change ◽  
Specific Proteins ◽  
Antigen Antibody ◽  
Machine Readable

Abstract A kinetic nephelometric method and instrument have been developed for the rapid determination of specific serum proteins by means of immunoprecipitin reactions. The maximum rate of change of scattered light intensity in an antigen-antibody reaction can be made to occur within 60 s after initiation of the reaction and provides a measure of the antigen concentration under antibody excess conditions. A mathematical relationship has been found for the conversion of the nonlinear maximum rate data directly into a linear concentration read-out, making possible the use of single-point calibration. Instrument operating parameters and computations are programmed for a particular analysis by means of machine-readable cards. Antigen-excess samples are detected rapidly by injection of calibrator into the reaction mixture after the rate signal has dropped to a pre-selected level. The method correlates well with both radial immunodiffusion and end-point nephelometric methods.

Improved continuous-flow (SMAC) determination of serum albumin.

1978 ◽  
Vol 24 (7) ◽  
pp. 1191-1193 ◽  
Author(s):  
G Cederblad ◽  
B E Hickey ◽  
A Hollender ◽  
G Akerlund
Keyword(s):  
Reaction Time ◽  
Serum Albumin ◽  
Acute Phase ◽  
Continuous Flow ◽  
Green Method ◽  
Acute Phase Reactants ◽  
Modified Method ◽  
Continuous Flow Method ◽  
Bromcresol Green

Abstract The albumin values determined by the bromcresol green methods do not compare well with values by more specific methods for albumin determination. The discrepancies have been related to, among other things, acute-phase reactants and are especially pronounced in the lower albumin range. These disadvantages are also inherent in a routine continuous-flow method for albumin (SMAC). The bromcresol green method has been improved considerably by shortening the reaction time before the absorbance is measured, as is described here. The modified method yields values that better agree with those by more specific methods and an influence of acute-phase reactants is no longer observed.

A Rapid Determination of Taurine in Human Plasma by LC

2009 ◽  
Vol 69 (11-12) ◽  
pp. 1427-1430 ◽  
Author(s):  
Mohammadreza Ghandforoush-Sattari ◽  
Siminozar Mashayekhi ◽  
Mahboub Nemati ◽  
Philipp A. Routledge
Keyword(s):  
Human Plasma ◽  
Rapid Determination
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