Urinary organic acids in man. I. Normal patterns.

1976 ◽  
Vol 22 (8) ◽  
pp. 1283-1287 ◽  
Author(s):  
A M Lawson ◽  
R A Chalmers ◽  
R W Watts
Keyword(s):  
Organic Acids ◽  
Human Urine ◽  
Organic Acidurias ◽  
Urinary Organic Acids ◽  
Qualitative Pattern

Abstract We studied qualitative pattern of urinary acidic metabolites excreted by normal persons. The results provide a basis on which to compare results for patients with potentially abnormal organic acidurias. A series of urinary polyhydroxy (aldonic and deoxyaldonic) acids has been identified. Most of these compounds have not been previously reported in human urine, except in connection with the present work, and are additional to the previously recognized urinary organic acids, which were also observed. Possible metabolic origins of some of the acids are briefly discussed.

Gas-chromatographic method of analysis for urinary organic acids. II. Description of the procedure, and its application to diagnosis of patients with organic acidurias.

1980 ◽  
Vol 26 (13) ◽  
pp. 1847-1853 ◽  
Author(s):  
K Tanaka ◽  
A West-Dull ◽  
D G Hine ◽  
T B Lynn ◽  
T Lowe
Keyword(s):  
Organic Acids ◽  
Chromatographic Method ◽  
Normal Subjects ◽  
Retention Indices ◽  
Organic Aciduria ◽  
Organic Acidurias ◽  
Urinary Organic Acids ◽  
Urinary Organic Acid Analysis ◽  
Gas Chromatographic Method ◽  
Extensive List

Abstract A gas-chromatographic method for urinary organic acid analysis is described, designed to be used routinely for the diagnosis of organic aciduria. It involves extraction of urine with ethyl acetate, dehydration of extract residues, and trimethylsilylation. Organic acids are identified by using an extensive list of retention indices published in the accompanying paper (this issue). Quantitative values are given for organic acids in urines from 50 ostensibly normal subjects. Typical chromatograms of urinary organic acids from patients with eight well-established organic acidurias are also shown.

Metabolic profiles of urinary organic acids recovered from absorbent filter paper.

1987 ◽  
Vol 33 (4) ◽  
pp. 572-576 ◽  
Author(s):  
B A Chamberlin ◽  
C C Sweeley
Keyword(s):  
Young Children ◽  
Organic Acids ◽  
Filter Paper ◽  
Metabolic Profiles ◽  
Sample Storage ◽  
Organic Acidurias ◽  
Urinary Organic Acids ◽  
Specimen Collection ◽  
Normal Urine

Abstract We evaluated for reliability and reproducibility a semiquantitative gas-chromatographic assay of organic acids in samples of normal urine recovered from absorbent filter paper. We also evaluated this method for use in diagnosis of some of the more common organic acidurias. Transfer of urine from diapers to absorbent filter paper eases the usual trauma of specimen collection from young children; it also simplifies sample storage and shipment.

scholarly journals Statistical Optimization of Urinary Organic Acids Analysis by a Multi-Factorial Design of Experiment

Analytica ◽  
2020 ◽  
Vol 1 (1) ◽  
pp. 14-23 ◽  
Author(s):  
Marco Pazzi ◽  
Sara Colella ◽  
Eugenio Alladio ◽  
M. Paola Puccinelli ◽  
Giulio Mengozzi ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Linear Regression ◽  
Organic Acids ◽  
Design Of Experiment ◽  
Inborn Errors Of Metabolism ◽  
Principal Component ◽  
Inborn Errors ◽  
Organic Acidurias ◽  
Urinary Organic Acids ◽  
High Yields

The analysis of urinary organic acids is useful for patients suspected to have inborn errors of metabolism known as organic acidurias. These diseases cause an accumulation of organic acids in body fluids and their abnormal excretion in urines. By means of chemometrics tools, such as principal component analysis and multiple linear regression, it was concluded that the conditions used in our laboratory are really the most suitable to achieve high yields of analytes.

scholarly journals GC-MS profiling of urinary organic acids evaluated as a quantitative method

1996 ◽  
Vol 42 (10) ◽  
pp. 1609-1615 ◽  
Author(s):  
P Duez ◽  
A Kumps ◽  
Y Mardens
Keyword(s):  
Organic Acids ◽  
Classical Method ◽  
Internal Standard ◽  
Selected Ion Monitoring ◽  
Practical Procedure ◽  
Urinary Organic Acids ◽  
Relative Standard ◽  
Analytical Response ◽  
Quantitative Results ◽  
Curvilinear Relationships

Abstract We assessed the quantitative performances of a classical method for profiling urinary organic acids: ethyl acetate extraction/oxime-trimethylsilyl derivatization/GC-MS. Twenty-seven acids were quantified on the basis of specific ions in both scan and selected-ion monitoring modes. We found that the tuning of the mass detector severely affects the calibration factors, being critical to achieve quantitative results, and we propose a practical procedure for reproducible tuning. Of seven compounds tested, tropic acid was retained as the internal standard suitable for most of the acids of clinical interest; a second internal standard, 2-ketocaproic acid, was used in quantifying keto-acids. The within-day and total relative standard deviations (CVs), estimated from scan-mode analyses of urine, ranged from 2.6% to 12.7% and from 4.2% to 11.8%, respectively. Curvilinear relationships between analytical response and concentration were observed for most of the acids investigated.

Recommendations for urinary organic acids analysis

2020 ◽  
Vol 78 (5) ◽  
pp. 547-554
Author(s):  
Laetitia Van Noolen ◽  
Cécile Acquaviva-Bourdain ◽  
Anne-Frédérique Dessein ◽  
Régine Minet-Quinard ◽  
Marie Nowoczyn ◽  
...  
Keyword(s):  
Organic Acids ◽  
Urinary Organic Acids
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