Determination of tricyclic antidepressant, clomipramine (Anafranil), in plasma by a specific radioimmunoassay procedure.

1978 ◽  
Vol 24 (1) ◽  
pp. 36-40 ◽  
Author(s):  
G F Read ◽  
D Riad-Fahmy
Keyword(s):  
Bovine Serum Albumin ◽  
Active Metabolite ◽  
Antidepressant Drug ◽  
Tricyclic Antidepressant ◽  
Chromatographic Purification ◽  
Throughput Rate ◽  
Specific Radioimmunoassay ◽  
Pharmacologically Active ◽  
Good Agreement

Abstract A radioimmunoassay is reported for the tricyclic antidepressant drug, clomipramine (Anafranil, Geigy). Antisera generated in rabbits to clomipramine conjugated to bovine serum albumin at positions 10 and 11 were specific, cross reacting less than 5% with the pharmacologically active metabolite, desmethylclomipramine. Specificity was confirmed by the good agreement in titres observed when the samples were assayed with and without a pre-assay thin-layer chromatographic purification. Intra- and inter-assay variations were less than 8 and 13%, respectively, with a sensitivity of 175 ng/liter. Results obtained agreed well with those reported by other groups using double radioisotope derivative assays. The method has a high throughput rate, one technician can assay 200 samples in duplicate in a working week; and it is sufficiently precise, sensitive, and specific for use in routine minitoring of the drug in plasma and for checking patient compliance with dosage regimen.

scholarly journals Simple, Rapid and Sensitive UV-Visible Spectrophotometric Method for Determination of Antidepressant Amitriptyline in Pharmaceutical Dosage Forms

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Pankaj Soni ◽  
Deepak Sinha ◽  
Rajmani Patel
Keyword(s):  
Antidepressant Drug ◽  
Pharmaceutical Preparations ◽  
Correlation Coefficients ◽  
Molar Absorptivity ◽  
Tricyclic Antidepressant ◽  
Simple Approach ◽  
Colour Intensity ◽  
Pharmaceutical Dosage Forms ◽  
Uv Visible

The paper describes a new and simple approach for spectrophotometric determination of tricyclic antidepressant drug amitriptyline. Enhancement of the colour intensity of the Fe(III)-SCN−complex on addition of the drug amitriptyline forms the basis of the proposed method. The value of molar absorptivity of the Fe(III)-SCN−amitriptyline ion pair complex in terms of the drug lies in the range of (2.82–3.36) × 103 L·mol−1·cm−1at the absorption maximum 460 nm. The detection limit of the method was 0.3 μg·mL−1. The slope, intercept, and correlation coefficients for the present method were found to be 0.008, 0.002, and +0.998, respectively. The effect of analytical variables on the determination of the drug and the composition of the complex are discussed in the paper. The method is applicable in the determination of amitriptyline in pharmaceutical preparations.

Assay of plasma testosterone during the first six months of life: importance of chromatographic purification of steroids

1995 ◽  
Vol 41 (8) ◽  
pp. 1146-1149 ◽  
Author(s):  
J S Fuqua ◽  
E S Sher ◽  
C J Migeon ◽  
G D Berkovitz
Keyword(s):  
Plasma Concentration ◽  
Ambiguous Genitalia ◽  
Plasma Testosterone ◽  
Plasma Samples ◽  
Chromatographic Purification ◽  
Direct Assay ◽  
Extraction And Purification ◽  
Over Time ◽  
Good Agreement

Abstract Determination of the plasma concentration of testosterone (T) is important in evaluating infants born with ambiguous genitalia and micropenis, and several commercially available kits provide a direct assay of T in unextracted plasma. Using plasma samples obtained from 36 subjects < 6 months old, we compared the concentration of plasma T measured by RIA after extraction and purification by column chromatography with the T concentration measured in a direct assay. When aliquots of samples were purified before RIA, the concentration of T was markedly lower than in the direct assay. In the first 3 weeks postpartum, results of the direct assay were 3.8-fold greater than those obtained after purification. This difference decreased over time, and by age 2 months there was fairly good agreement between the two methods. These data indicate that some direct assays of plasma T are inappropriate during the first 2 months postpartum.

scholarly journals Determination of Lappaconitin, Diterpene Alkaloide Obtained from Plants Aconitum leucostomum, and its Active Metabolite N-desacetyllappaconitin in Human Plasma and Blood

2021 ◽  
Vol 10 (3) ◽  
pp. 105-113
Author(s):  
O. A. Archakova ◽  
T. N. Komarov ◽  
A. V. Rogov ◽  
D. S. Shchelgacheva ◽  
A. A. Aleshina ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Human Plasma ◽  
Active Metabolite ◽  
High Performance ◽  
Limit Of Quantification ◽  
Carry Over ◽  
Pharmacologically Active ◽  
Effective Drugs ◽  
Active Substances

Introduction. Lappaconitine is an alkaloid, contained into Aconitum leucostomum Vorosh. roots and herbs. The alkaloid is indicated to arrhythmia. The lappaconitine drugs are metabolized into eight pharmacologically active substances, but N-desacetyllappaconitine is the most effective. Drugs based on a lappaconitine has narrow therapeutic range and many kinds of side effects. Pharmacokinetics of lappaconitine should be more studied for safety medical use of lappaconitine drugs.Aim. The aim of this study is to develop method for the quantitative determination of lappaconitine and its active metabolite N-desacetyllappaconitine in human plasma and blood by high performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS). Materials and methods. Determination of lappaconitine and N-desacetyllappaconitine in plasma and blood was carried out by HPLC-MS/MS. The samples were processed by acetonitrile protein precipitation.Results and discussion. This method was validated by next parameters: selectivity, matrix effect, calibration curve, accuracy, precision, spike recovery, lower limit of quantification, carry-over effect and stability.Conclusion. The method of the quantitative determination of lappaconitine and N-desacetyllappaconitine in human plasma and blood was developed and validated by HPLC-MS/MS. The analytical range of the was 0.50-50.00 ng/ml for lappaconitine and 0.50-100.00 ng/ml for N-desacetyllappaconitine in biological matrix. Method could be applied to determination of lappaconitine and N-desacetyllappaconitine for PK studies.

COMPARATIVE ACTION OF VARIOUS OESTROGENIC COMPOUNDS ON MOUSE VAGINAL SIALIC ACIDS (II)

1969 ◽  
Vol 62 (4) ◽  
pp. 663-670 ◽  
Author(s):  
Lars Carlborg
Keyword(s):  
Sialic Acid ◽  
Response Curve ◽  
Sialic Acids ◽  
Clear Cut ◽  
Suitable Parameter ◽  
Sufficient Degree ◽  
Comparative Action ◽  
Relative Potencies ◽  
Good Agreement

ABSTRACT Oestrogens administered in lower doses than necessary to induce full cornification of the mouse vagina induce mucification. It was shown previously that the degree of mucification could be estimated by quantitative determination of sialic acids. A suitable parameter for oestrogen assay was the measurement of vaginal sialic acid concentration which exhibited a clear cut dose response curve. Eleven assays of various oestrogens were performed with this method. Their estimated relative potencies were in good agreement with other routine oestrogen assays. A statistically sufficient degree of precision was found. The sensitivity was of the same order, or slightly higher, than the Allen-Doisy test.

Evaluation of Myocardial Cell Damage by In-111-Monoclonal Antimyosin Antibodies in Patients Under Chronic Tricyclic Antidepressant Drug Treatment

Circulation ◽  
1995 ◽  
Vol 91 (6) ◽  
pp. 1619-1623 ◽  
Author(s):  
Vicens Martí ◽  
Manel Ballester ◽  
Claudi Udina ◽  
Ignasi Carrió ◽  
Enric Alvarez ◽  
...  
Keyword(s):  
Drug Treatment ◽  
Cell Damage ◽  
Antidepressant Drug ◽  
Myocardial Cell ◽  
Tricyclic Antidepressant ◽  
Antidepressant Drug Treatment ◽  
Myocardial Cell Damage

An Automated Method for the Determination of Serum Calcium with Glyoxal Bis (2-Hydroxyanil)

1967 ◽  
Vol 13 (6) ◽  
pp. 515-520 ◽  
Author(s):  
Genevieve Farese ◽  
Janice L Schmidt ◽  
Milton Mager
Keyword(s):  
Serum Calcium ◽  
Automated Analysis ◽  
Automated Method ◽  
Good Agreement

Abstract A completely automated analysis is described for the determination of serum calcium with glyoxal bis (2-hydroxyanil) solution (GBHA). The method is simple and precise, and the data obtained are in good agreement with results obtained by the manual GBHA procedure.

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