Liquid-chromatographic analysis for common tricyclic antidepressant drugs and their metabolites in serum or plasma with the technicon "FAST-LC" system.

1981 ◽  
Vol 27 (6) ◽  
pp. 849-855 ◽  
Author(s):  
S J Bannister ◽  
S van der Wal ◽  
J W Dolan ◽  
L R Snyder
Keyword(s):  
High Performance ◽  
Antidepressant Drugs ◽  
Total Sample ◽  
Sample Volume ◽  
Tricyclic Antidepressant ◽  
Tricyclic Antidepressant Drugs ◽  
Liquid Chromatographic Analysis ◽  
Double Extraction ◽  
Liquid Chromatographic ◽  
Total Sample Volume

Abstract We describe a single procedure for assay of seven tricyclic antidepressant drugs and metabolites in serum or plasma: protriptyline, nortriptyline, amitriptyline, desmethyldoxepin, doxepin, desipramine, and imipramine. With the Technicon "FAST-LC" system, samples are aspirated directly into the unit and pretreated via double extraction; the concentration of each drug is then determined by "high-performance" liquid chromatography. Final chromatograms are monitored at 205 nm, at analysis rates of 7.5 samples/h. Concentration and absorbance are linearly related for each drug from 0 to 1400 micrograms/L. Day-to-day CVs averaged 5 to 6% for each drug, and there is good correlation of FAST-LC values with those obtained by gas-chromatographic methods. Total sample volume is 750 microliters.

On-line liquid-chromatographic analysis for drugs in serum with the Technicon "FAST-LC" system: performance data for theophylline and for four commonly used anticonvulsants and their metabolites.

1980 ◽  
Vol 26 (7) ◽  
pp. 871-880 ◽  
Author(s):  
J W Dolan ◽  
S van der Wal ◽  
S J Bannister ◽  
L R Snyder
Keyword(s):  
High Performance ◽  
Total Sample ◽  
Sample Volume ◽  
Active Metabolites ◽  
Serum Samples ◽  
New Instrument ◽  
On Line ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic ◽  
Total Sample Volume

Abstract We describe a new instrument for use in assay of therpeutic drugs in serum by "high-performance" liquid chromatography, the "FAST-LC" system (Technicon). Serum samples are aspirated directly into the unit, extracted with solvent, and the evaporated and redissolved extract is injected onto a chromatographic column. We illustrate the performance of the system by assays in serum for theophylline and four anticonvulsants (primidone, phenobarbital, phenytoin, and carbamazepine) plus two of their active metabolites (phenylethylmalonamide and carbamazepine epoxide). For theophylline, final chromatograms are monitored at 270 nm, at analysis rates of 10/h. Concentration and absorbance are linearly related from 0 to 130 mg of theophylline per liter. For the anticonvulsants, chromatograms are monitored at 200 nm, at analysis rates of 7.5/h. The six individual determinations are each linear beyond the therapeutic range. For both drug panels, day-to-day CV's were 4 to 6%. Results correlate well with those by enzyme immunoassay. A total sample volume of 150 microL is required.

On-line liquid-chromatographic analysis for drugs in serum with the Technicon "FAST-LC" system: performance data for theophylline and for four commonly used anticonvulsants and their metabolites.

1980 ◽  
Vol 26 (7) ◽  
pp. 871-880
Author(s):  
J W Dolan ◽  
S van der Wal ◽  
S J Bannister ◽  
L R Snyder
Keyword(s):  
High Performance ◽  
Total Sample ◽  
Sample Volume ◽  
Active Metabolites ◽  
Serum Samples ◽  
New Instrument ◽  
On Line ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic ◽  
Total Sample Volume

Abstract We describe a new instrument for use in assay of therpeutic drugs in serum by "high-performance" liquid chromatography, the "FAST-LC" system (Technicon). Serum samples are aspirated directly into the unit, extracted with solvent, and the evaporated and redissolved extract is injected onto a chromatographic column. We illustrate the performance of the system by assays in serum for theophylline and four anticonvulsants (primidone, phenobarbital, phenytoin, and carbamazepine) plus two of their active metabolites (phenylethylmalonamide and carbamazepine epoxide). For theophylline, final chromatograms are monitored at 270 nm, at analysis rates of 10/h. Concentration and absorbance are linearly related from 0 to 130 mg of theophylline per liter. For the anticonvulsants, chromatograms are monitored at 200 nm, at analysis rates of 7.5/h. The six individual determinations are each linear beyond the therapeutic range. For both drug panels, day-to-day CV's were 4 to 6%. Results correlate well with those by enzyme immunoassay. A total sample volume of 150 microL is required.

Quantitative liquid-chromatographic technique for the simultaneous assay of tricyclic antidepressant drugs in plasma or serum.

1982 ◽  
Vol 28 (3) ◽  
pp. 457-461 ◽  
Author(s):  
P K Sonsalla ◽  
T A Jennison ◽  
B S Finkle
Keyword(s):  
Antidepressant Drugs ◽  
Isoamyl Alcohol ◽  
Solvent System ◽  
Tricyclic Antidepressant ◽  
Chromatographic Technique ◽  
Internal Standards ◽  
Tricyclic Antidepressant Drugs ◽  
Drug Concentrations ◽  
Micrometer Particle ◽  
Liquid Chromatographic

Abstract A quantitative "high-pressure" liquid-chromatographic assay for tricyclic antidepressant drugs in plasma or serum is described in which amitriptyline, nortriptyline, imipramine, desipramine, doxepin, and desmethyldoxepin are separated with a 10-micrometer particle size silica column and a methanol/NH4OH/NH4NO3 solvent system. The drugs and two internal standards are extracted with hexane/isoamyl alcohol, the solvent layer is evaporated at 40 degrees C, and the drugs are detected at 254 nm. Drug concentrations are linear with absorbance from 25 to 1000 micrograms/L; within-assay and between-assay CVs are less than or equal to 10%.

Sample preparation and liquid-chromatographic analysis for tricyclic antidepressants in serum.

1982 ◽  
Vol 28 (3) ◽  
pp. 462-466 ◽  
Author(s):  
P Koteel ◽  
R E Mullins ◽  
R H Gadsden
Keyword(s):  
Detection Limit ◽  
Sample Preparation ◽  
Extraction Procedure ◽  
Antidepressant Drugs ◽  
Tricyclic Antidepressant ◽  
Hexane Extraction ◽  
Sample Extraction ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract We report two separate sample-preparation procedures and a "high-pressure" liquid-chromatographic method for quantitating nine tricyclic antidepressant drugs: trimipramine, doxepin, amitriptyline, imipramine, desmethyldoxepin, nortriptyline, desipramine, maprotiline, and protriptyline. The drugs are extracted from 1 mL of serum by a single manual hexane extraction or by using DuPont's PREP I Automated Sample Processor. The drugs from either process are chromatographed on a mu-Bondapak-CN column with a mobile phase of acetonitrile/methanol/Na2HPO4, 5 mmol/L (60/15/25 by vol), pH 7.0, at a flow rate of 2.0 mL/min. Absorbance at 254 nm is linear with drug concentration from 25 to 1000 mg/L for each drug. With the manual extraction procedure the detection limit was 10 mg/L, absolute recovery 20-44%, within-run precision (CV) 10.1%, and run-to-run precision 11.1%. The performance of automated sample extraction was better, with a detection limit of 5 mg/l, absolute recovery 72-97%, within-run precision 6.1%, and run-to-run precision 6.6%.

Sign in / Sign up

Export Citation Format

Share Document