High-density lipoprotein subfractions as measured by differential polyanionic precipitation and rate zonal ultracentrifugation.

1982 ◽  
Vol 28 (10) ◽  
pp. 2040-2043 ◽  
Author(s):  
H S Simpson ◽  
F C Ballantyne ◽  
C J Packard ◽  
H G Morgan ◽  
J Shepherd
Keyword(s):  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Protective Role ◽  
High Density ◽  
Lipoprotein Subfractions ◽  
Functional Roles ◽  
High Density Lipoprotein Subfractions ◽  
Hdl2 Cholesterol

Abstract Recent interest in the putative protective role of high-density lipoprotein (HDL) and its subfractions against atherosclerosis has highlighted the need for a rapid, simple subfractionation procedure. Here we compared HDL subfractionation by two recently developed polyanionic-precipitation methods with the values obtained by rate zonal ultracentrifugation. A similar result for total HDL cholesterol was obtained by all three methods. However, HDL2 cholesterol as measured by the precipitation procedures was significantly higher than the zonal value, and HDL3 was lower. This reflects the different underlying principles involved in the separations and highlights the need for a clearer understanding of the functional roles of the HDL fractions.

Cholesterol distribution between high-density-lipoprotein subfractions HDL2 and HDL3 determined in serum by discontinuous gradient gel electrophoresis

1991 ◽  
Vol 37 (7) ◽  
pp. 1149-1152 ◽  
Author(s):  
Véronique Atger ◽  
Denise Malon ◽  
Marie Claude Bertiere ◽  
Françoise N'Diaye ◽  
Anik Girard-Globa
Keyword(s):  
High Density Lipoprotein ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
High Density ◽  
Lipoprotein Subfractions ◽  
Cylindrical Tubes ◽  
High Density Lipoprotein Subfractions ◽  
Gradient Gel Electrophoresis ◽  
Hdl2 Cholesterol

Abstract We used discontinuous gradients of polyacrylamide gel to determine the high-density-lipoprotein (HDL) subfractions HDL2 and HDL3 of serum lipoproteins. Serum (40 microL) prestained with Sudan Black was electrophoresed in cylindrical tubes over successive layers of 3.5%, 6%, 13%, and 17.5% acrylamide gels in a Tris.glycine buffer (3-4 h, 300 V). Very-low- (VLDL) and low-density lipoprotein (LDL) were retained by the 3.5% and 6% gels. HDL2 was concentrated at the interface between the 13% and 17.5% gels, and HDL3 migrated into the 17.5% gel. The distribution between HDL2 and HDL3 was obtained by densitometric scanning. Application of the respective percentages to HDL cholesterol assayed after phosphotung-state-Mg2+ precipitation of VLDL and LDL gave calculated concentrations of HDL2 and HDL3 cholesterol. The calculated values for HDL2 cholesterol were in excellent agreement with those for HDL2 isolated by ultracentrifugation (r = 0.920 for n = 120 sera; differences nonsignificant by Student's paired t-test). Besides being highly discriminating, the method is rapid, easily performed, and economical.

High-density lipoprotein subfractions measured in stored serum

1994 ◽  
Vol 40 (9) ◽  
pp. 1713-1716 ◽  
Author(s):  
L L Bausserman ◽  
A L Saritelli ◽  
D Milosavljevic
Keyword(s):  
High Density Lipoprotein ◽  
Dextran Sulfate ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
High Density ◽  
Manganese Chloride ◽  
Lipoprotein Subfractions ◽  
Hdl Subfractions ◽  
High Density Lipoprotein Subfractions

Abstract We compared the effects of freezing serum on the determination of high-density lipoprotein (HDL) subfractions by two dual-precipitation methods, heparin and manganese chloride/dextran sulfate (HM/DS) (Gidez et al., J Lipid Res 1982;23:1206-23) and DS/DS (Warnick et al., Clin Chem 1982;28:1574), and by ultracentrifugation. Storing serum for 1 month at -70 degrees C resulted in reduced HDL3-cholesterol by ultracentrifugation and reduced total and HDL3-cholesterol by the DS/DS method. There was no change in either total HDL-cholesterol or HDL3-cholesterol with the HM/DS method. Additional studies involving only HM/DS indicated that total HDL-cholesterol in serum stored at 4 degrees C begins to decline after 3 days (-3.1 +/- 3.5%, P < 0.1). HDL was stable at -20 degrees C for 2 weeks but both total and HDL3-cholesterol decreased significantly after 1 month. Storage of serum at -70 degrees C resulted in no changes for 1 year; however, at 18 months, HDL3-cholesterol was reduced 13% (P = 0.002). We conclude that HDL subfractions can be determined accurately in serum as well as in plasma after storage at -70 degrees C for up to 1 year.

Alterations in high-density lipoprotein subfractions during postprandial lipidaemia induced by fat with and without ethanol

1988 ◽  
Vol 75 (2) ◽  
pp. 135-142 ◽  
Author(s):  
G. Franceschini ◽  
Y. Moreno ◽  
P. Apebe ◽  
L. Calabresi ◽  
E. Gatti ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
High Density ◽  
Acute Administration ◽  
Cholesteryl Esters ◽  
Lipoprotein Subfractions ◽  
Apo B ◽  
Postprandial Phase ◽  
High Density Lipoprotein Subfractions

1. Serum lipid and apolipoprotein levels, distribution and composition of high-density lipoprotein (HDL) sub-fractions and lecithin:cholesterol acryltransferase activity were analysed in nine normolipidaemic subjects, in whom a hypertriglyceridaemic state was induced by the acute administration of ethanol (40 g) plus fat (70 g) or of fat only. 2. Triglyceride (TG) levels increased by 180% 4–6 h after fat plus ethanol intake, the hypertriglyceridaemic response being inversely correlated with the basal HDL2 mass (r = −0.82). Serum apolipoprotein (apo) B levels rose by 8%, HDL–cholesterol decreased by 10% and HDL–TG increased by 57% at 6–8 h. 3. When ethanol was omitted, serum cholesterol and TG rose by 6% and 70%, respectively; both apo AI and apo B levels went up by 8%, whereas HDL-cholesterol rose progressively (15%) at 12 h. 4. The flotation rates of both HDL2 and HDL, increased, reaching a maximum 6–8 h after ethanol plus fat intake. These changes were due to an increase in TG and phospholipid contents, whereas cholesteryl esters and proteins decreased. 5. The alterations in HDL are attributable to the increase in TG-rich lipoproteins, to the stimulated cholesterol esterification (+ 15%) and to an enhanced transfer of newly formed cholesteryl esters to apo-B-containing lipoproteins in exchange for TG. 6. Changes in HDL properties were evident only when ethanol was given concomitantly with fat. 7. These findings suggest that in the postprandial phase lipoprotein changes may occur, which facilitate an improved removal of cholesterol from tissues.

A dual-precipitation method evaluated for measurement of cholesterol in high-density lipoprotein subfractions HDL2 and HDL3 in human plasma.

1989 ◽  
Vol 35 (2) ◽  
pp. 265-270 ◽  
Author(s):  
W Patsch ◽  
S A Brown ◽  
J D Morrisett ◽  
A M Gotto ◽  
J R Patsch
Keyword(s):  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
Cholesterol Content ◽  
Epidemiological Studies ◽  
High Density ◽  
Cholesterol Concentration ◽  
Precipitation Method ◽  
Lipoprotein Subfractions ◽  
High Density Lipoprotein Subfractions ◽  
Hdl2 Cholesterol

Abstract The dual-precipitation method for measurement of cholesterol in high-density lipoprotein subfractions HDL2 and HDL3 (Warnick et al., Clin Chem 1982;28:1574) was compared with quantification of cholesterol in HDL2 and HDL3 by zonal ultracentrifugation (Patsch et al., J Lipid Res 1974;15:356-66). For 39 plasma specimens differing widely in their HDL subfraction cholesterol concentration, the coefficient of correlation between the two methods was 0.94 for HDL2-cholesterol, 0.82 for HDL3-cholesterol. Storage of plasma specimens at -70 degrees C decreased the apparent content of HDL3-cholesterol by 5%; no significant changes in HDL2-cholesterol were observed. In frozen plasma, interference by apoB-containing lipoproteins and by lipoprotein(a) was negligible. Mean weight ratios of apoA-I to cholesterol were twice as high for HDL3 as for HDL2, reflecting the increased cholesterol content of HDL2. The study suggests that quantification of HDL2 and HDL3 cholesterol by precipitation is appropriate for use in epidemiological studies.

scholarly journals Novel Insights From Human Studies on the Role of High-Density Lipoprotein in Mortality and Noncardiovascular Disease

2020 ◽  
Author(s):  
Christian M. Madsen ◽  
Anette Varbo ◽  
Børge G. Nordestgaard
Keyword(s):  
Cardiovascular Disease ◽  
High Density Lipoprotein ◽  
Therapeutic Potential ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
High Density ◽  
Cancer Type ◽  
Health And Disease ◽  
And Function

The vast majority of research about HDL (high-density lipoprotein) has for decades revolved around the possible role of HDL in atherosclerosis and its therapeutic potential within cardiovascular disease prevention; however, failures with therapies aimed at increasing HDL cholesterol has left questions as to what the role and function of HDL in human health and disease is. Recent observational studies have further shown that extreme high HDL cholesterol is associated with high mortality leading to speculations that HDL could in some instances be harmful. In addition, evidence from observational, and to a lesser extent genetic, studies has emerged indicating that HDL might be associated with the development of other major noncardiovascular diseases, such as infectious disease, autoimmune disease, cancer, type 2 diabetes, kidney disease, and lung disease. In this review, we discuss (1) the association between extreme high HDL cholesterol and mortality and (2) the emerging human evidence linking HDL to several major diseases outside the realm of cardiovascular disease.

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