Effect of short-term storage conditions on alcohol concentrations in blood from living human subjects.

1983 ◽  
Vol 29 (11) ◽  
pp. 1959-1960 ◽  
Author(s):  
C L Winek ◽  
L J Paul
Keyword(s):  
Gas Chromatography ◽  
Sodium Fluoride ◽  
Whole Blood ◽  
Experimental Error ◽  
Human Subjects ◽  
Storage Conditions ◽  
Short Term ◽  
Short Term Storage ◽  
Term Storage

Abstract We examined the effects of time, temperature, and a preservative (sodium fluoride) on ethanol concentrations in stored samples of whole blood from living human subjects. We measured the ethanol in the first, second, seventh, and 14th day of storage, by gas chromatography. Samples were stored at 0-3 degrees C and at 22-29 degrees C, with and without preservative. None of these showed significant gains or losses in concentration. The average differences between ethanol as measured on the day of collection and after storage were all within the range of experimental error of the method (+/- 5%).

scholarly journals Impact of Thermotherapy and Short-Term Storage on Quercus robur L. Acorn Mycobiota and Germination

Forests ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 528
Author(s):  
Jelena Kranjec Orlović ◽  
Damir Drvodelić ◽  
Marko Vukelić ◽  
Matea Rukavina ◽  
Danko Diminić ◽  
...  
Keyword(s):  
Natural Regeneration ◽  
Fungal Diversity ◽  
Quercus Robur ◽  
Negative Impact ◽  
Storage Conditions ◽  
Short Term ◽  
Isolation Frequency ◽  
Short Term Storage ◽  
Penicillium Spp ◽  
Term Storage

When natural regeneration of Quercus robur stands is hampered by an insufficient acorn yield, human assisted sowing of acorns collected in non-affected stands and stored for some period of time is performed. To inhibit the development of fungi and acorn deterioration during storage, thermotherapy is usually applied by submerging acorns for 2.5 h in water heated to 41 °C. This research aimed to test the effect of four thermotherapy treatments of different durations and/or applied temperatures as well as short-term storage at −1 °C or 3 °C on acorn internal mycobiota and germination. Fungal presence in cotyledons was analyzed in 450 acorns by isolation of mycelia on artificial media, followed by a DNA-based identification. Germination of 2000 acorns was monitored in an open field trial. Thermotherapy significantly decreased fungal diversity, while storage at 3 °C increased the isolation frequency of several fungi, mainly Penicillium spp. The most frequently isolated fungi did not show a negative impact on acorn germination after short-term storage. The study confirmed the efficiency of thermotherapy in the eradication of a part of acorn internal mycobiota, but also its effect on the proliferation of fast-colonizing fungi during storage. However, the latter showed to be more stimulated by storage conditions, specifically by storage at 3 °C.

scholarly journals Management of Apple Maturity and Postharvest Storage Conditions to Increase Polyphenols in Cider

HortScience ◽  
2019 ◽  
Vol 54 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Brianna L. Ewing ◽  
Gregory M. Peck ◽  
Sihui Ma ◽  
Andrew P. Neilson ◽  
Amanda C. Stewart
Keyword(s):  
The United States ◽  
Storage Conditions ◽  
Short Term ◽  
Postharvest Storage ◽  
Total Polyphenols ◽  
Fruit Storage ◽  
Long Term Storage ◽  
Short Term Storage ◽  
Term Storage

Hard cider production in the United States has increased dramatically during the past decade, but there is little information on how harvest and postharvest practices affect the chemistry of the resulting cider, including concentrations of organoleptically important flavanols. For 2 years we assessed fruit, juice, and cider from a total of five apple (Malus ×domestica Borkh.) cultivars in two experiments: sequential harvests and postharvest storage. Different cultivars were used in 2015 and 2016 with the exception of ‘Dabinett’, which was assessed in both years. There were no differences in polyphenol concentrations in cider made from fruit that was harvested on three separate occasions over a 4-week period in either 2015 or 2016. Fruit storage durations and temperatures had little influence on the chemistry when the experiment was conducted in 2015, but polyphenol concentration was greater after storage in the 2016 experiment. In 2016, total polyphenols in ‘Dabinett’ ciders were 51% greater after short-term storage at 10 °C and 67% greater after long-term storage at 1 °C than the control, which was not subjected to a storage treatment. In 2016, total polyphenols in ‘Binet Rouge’ ciders were 67% greater after short-term storage at 10 °C and 94% greater after long-term storage at 1 °C than the control. Although results varied among cultivars and harvest years, storing apples for longer periods of time and at warmer temperatures may be a strategy to increase polyphenol, particularly flavanol, concentrations in hard cider.

scholarly journals Integrating Whole Blood Transcriptomic Collection Procedures Into the Current Anti-Doping Testing System, Including Long-Term Storage and Re-Testing of Anti-Doping Samples

2021 ◽  
Vol 8 ◽  
Author(s):  
Giscard Lima ◽  
Alexander Kolliari-Turner ◽  
Fernanda Rossell Malinsky ◽  
Fergus M. Guppy ◽  
Renan Paulo Martin ◽  
...  
Keyword(s):  
Whole Blood ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Short Term ◽  
Long Term Storage ◽  
Short Term Storage ◽  
Short And Long Term ◽  
Rna Preservation ◽  
Term Storage

Introduction: Recombinant human erythropoietin (rHuEPO) administration studies involving transcriptomic approaches have demonstrated a gene expression signature that could aid blood doping detection. However, current anti-doping testing does not involve collecting whole blood into tubes with RNA preservative. This study investigated if whole blood in long-term storage and whole blood left over from standard hematological testing in short-term storage could be used for transcriptomic analysis despite lacking RNA preservation.Methods: Whole blood samples were collected from twelve and fourteen healthy nonathletic males, for long-term and short-term storage experiments. Long-term storage involved whole blood collected into Tempus™ tubes and K2EDTA tubes and subjected to long-term (i.e., ‒80°C) storage and RNA extracted. Short-term storage involved whole blood collected into K2EDTA tubes and stored at 4°C for 6‒48 h and then incubated at room temperature for 1 and 2 h prior to addition of RNA preservative. RNA quantity, purity, and integrity were analyzed in addition to RNA-Seq using the MGI DNBSEQ-G400 on RNA from both the short- and long-term storage studies. Genes presenting a fold change (FC) of >1.1 or < ‒1.1 with p ≤ 0.05 for each comparison were considered differentially expressed. Microarray analysis using the Affymetrix GeneChip® Human Transcriptome 2.0 Array was additionally conducted on RNA from the short-term study with a false discovery ratio (FDR) of ≤0.05 and an FC of >1.1 or < ‒1.1 applied to identify differentially expressed genes.Results: RNA quantity, purity, and integrity from whole blood subjected to short- and long-term storage were sufficient for gene expression analysis. Long-term storage: when comparing blood tubes with and without RNA preservation 4,058 transcripts (6% of coding and non-coding transcripts) were differentially expressed using microarray and 658 genes (3.4% of mapped genes) were differentially expressed using RNA-Seq. Short-term storage: mean RNA integrity and yield were not significantly different at any of the time points. RNA-Seq analysis revealed a very small number of differentially expressed genes (70 or 1.37% of mapped genes) when comparing samples stored between 6 and 48 h without RNA preservative. None of the genes previously identified in rHuEPO administration studies were differently expressed in either long- or short-term storage experiments.Conclusion: RNA quantity, purity, and integrity were not significantly compromised from short- or long-term storage in blood storage tubes lacking RNA stabilization, indicating that transcriptomic analysis could be conducted using anti-doping samples collected or biobanked without RNA preservation.

scholarly journals Evaluation of sweet cherry fruit quality after short-term storage in relation to the rootstock

2019 ◽  
Vol 60 (6) ◽  
pp. 925-934 ◽  
Author(s):  
Ewa Dziedzic ◽  
Jan Błaszczyk
Keyword(s):  
Fruit Quality ◽  
Sweet Cherry ◽  
Fungal Infections ◽  
Weather Conditions ◽  
Storage Conditions ◽  
Soluble Solids ◽  
Short Term ◽  
Fruit Storage ◽  
Short Term Storage ◽  
Term Storage

Abstract Fruits of the sweet cherry cultivar ‘Regina’ collected from trees growing on seven rootstocks were stored in a cold room at 2 °C with a normal (NA) and controlled atmosphere (15% and 20% CO2 and 5% O2—CA1 and CA2) for 2 weeks. The rootstocks on which the trees grew and the storage conditions significantly affected all fruit parameters tested during both years of the experiment. Fruit from Damil rootstock exhibited higher mean firmness than fruit from Colt rootstock. The effect of rootstocks on the value of soluble solids content (SSC) varied, wherein the fruits from Tabel Edabriz and Damil were characterized by high SSC mean content. The organic acids content (TA) was significantly lower after storage than during harvest time. Fruits from Tabel Edabriz trees were characterized by faster ripening, as was evident by the higher SSC to TA ratio. The amount of mass lost depended significantly and only on the storage conditions—sweet cherries from both CA combinations had the lowest mass losses. The percentage of fruits showing disease symptoms was largely dependent on the weather conditions in the orchard the year before the fruit harvest, as well as atmosphere composition and RH during fruit storage. Cold storage conditions with a high (20%) CO2 content are recommended for the short-term storage of sweet cherry fruits because they preserve fruit quality parameters: a low decrease in firmness, maintenance of a high SSC/TA ratio, a low percent of fungal infections, and good preservation of green color in the peduncle.

scholarly journals Short-term Storage of Plug-grown Bedding Plant Seedlings

HortScience ◽  
1992 ◽  
Vol 27 (7) ◽  
pp. 798-800 ◽  
Author(s):  
M.P. Kaczperski ◽  
A.M. Armitage
Keyword(s):  
Petunia Hybrida ◽  
Storage Period ◽  
Storage Conditions ◽  
Production Environment ◽  
Short Term ◽  
Salvia Splendens ◽  
Bedding Plant ◽  
Short Term Storage ◽  
Plant Seedlings ◽  
Term Storage

The effects of storage conditions before transplanting were examined for Petunia × hybrida Vilm. `Supercascade Lilac', viola × wittrockiana Gams `Universal Beaconsfield', and Salvia splendens F. Sellow ex Roem. & Schult `Red Hot Sally'. Plug grown seedlings were stored for 0, 7, 14, or 21 days at 5 or 10C and with continuous irradiance levels from incandescent bulbs at 0, 2, or 12 μmol·m-2·s-1. A second group was stored at 18C with irradiance from fluorescent bulbs at 105 μmol·m-2·s-1 and a 16-hour photoperiod for the same durations. Temperature was more important than irradiance in maintaining a commercially acceptable plant during the storage period. Petunia and pansy could be stored successfully for 21 days at 5 or 10C with no appreciable loss of quality; salvia could be stored for a minimum of 14 days. Seedlings of all species elongated excessively when stored >7 days at 18C and 105 μmol·m-2·s-1 irradiance. After 14 days of storage, petunias stored at 18C flowered sooner than those stored at 5 or 10C but time in a production environment (days to flower - days in storage) was similar for petunias stored at 5 or 18C.

scholarly journals The effect of short term storage on different winter wheat varieties rheological properties

2014 ◽  
pp. 83-86
Author(s):  
Mariann Móré ◽  
Gerda Diósi ◽  
Zoltán Győri ◽  
Péter Sipos
Keyword(s):  
Storage Conditions ◽  
Rheological Parameters ◽  
Short Term ◽  
Wheat Varieties ◽  
Short Term Storage ◽  
Stability Time ◽  
The University ◽  
Term Storage ◽  
Winter Wheat Varieties

The aim of storage after harvest is to protect the quality of wheat, because after-ripening occurs in the first 5–6 weeks. During this time it very important to make the optimal storage conditions. We have carried out storage experiment with wheat samples from Látókép Research Farm of the University of Debrecen. We analyzed the rheological parameters of Lupus and GK Csillag varieties from the crop year 2011/2012. The experiment period was between July and August 2012 (24. 07. 2012., 31. 07. 2012., 21. 08. 2012.).We determined the rheological parameters (water absorption, dough stability time and valorigraph quality number) of Lupus and GK Csillag during short term storage. Our results showed that after-ripening increased the baking quality of Lupus and GK Csillag during storage.

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