Influence of short-term storage conditions on the stability of total protein concentrations and electrophoretic fractions in plasma samples from loggerhead sea turtles, Caretta caretta

2014 ◽  
Vol 24 (5) ◽  
pp. 1091-1095 ◽  
Author(s):  
Daniela Alberghina ◽  
Simona Marafioti ◽  
Filippo Spadola ◽  
Michele Panzera ◽  
Giuseppe Piccione
1994 ◽  
Vol 348 (10) ◽  
pp. 696-698 ◽  
Author(s):  
Manfred Volz ◽  
Gisela Gottschalk ◽  
Hussein Husseini ◽  
Veselin Mitrovic ◽  
Martin Schlepper

Forests ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 528
Author(s):  
Jelena Kranjec Orlović ◽  
Damir Drvodelić ◽  
Marko Vukelić ◽  
Matea Rukavina ◽  
Danko Diminić ◽  
...  

When natural regeneration of Quercus robur stands is hampered by an insufficient acorn yield, human assisted sowing of acorns collected in non-affected stands and stored for some period of time is performed. To inhibit the development of fungi and acorn deterioration during storage, thermotherapy is usually applied by submerging acorns for 2.5 h in water heated to 41 °C. This research aimed to test the effect of four thermotherapy treatments of different durations and/or applied temperatures as well as short-term storage at −1 °C or 3 °C on acorn internal mycobiota and germination. Fungal presence in cotyledons was analyzed in 450 acorns by isolation of mycelia on artificial media, followed by a DNA-based identification. Germination of 2000 acorns was monitored in an open field trial. Thermotherapy significantly decreased fungal diversity, while storage at 3 °C increased the isolation frequency of several fungi, mainly Penicillium spp. The most frequently isolated fungi did not show a negative impact on acorn germination after short-term storage. The study confirmed the efficiency of thermotherapy in the eradication of a part of acorn internal mycobiota, but also its effect on the proliferation of fast-colonizing fungi during storage. However, the latter showed to be more stimulated by storage conditions, specifically by storage at 3 °C.


HortScience ◽  
2019 ◽  
Vol 54 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Brianna L. Ewing ◽  
Gregory M. Peck ◽  
Sihui Ma ◽  
Andrew P. Neilson ◽  
Amanda C. Stewart

Hard cider production in the United States has increased dramatically during the past decade, but there is little information on how harvest and postharvest practices affect the chemistry of the resulting cider, including concentrations of organoleptically important flavanols. For 2 years we assessed fruit, juice, and cider from a total of five apple (Malus ×domestica Borkh.) cultivars in two experiments: sequential harvests and postharvest storage. Different cultivars were used in 2015 and 2016 with the exception of ‘Dabinett’, which was assessed in both years. There were no differences in polyphenol concentrations in cider made from fruit that was harvested on three separate occasions over a 4-week period in either 2015 or 2016. Fruit storage durations and temperatures had little influence on the chemistry when the experiment was conducted in 2015, but polyphenol concentration was greater after storage in the 2016 experiment. In 2016, total polyphenols in ‘Dabinett’ ciders were 51% greater after short-term storage at 10 °C and 67% greater after long-term storage at 1 °C than the control, which was not subjected to a storage treatment. In 2016, total polyphenols in ‘Binet Rouge’ ciders were 67% greater after short-term storage at 10 °C and 94% greater after long-term storage at 1 °C than the control. Although results varied among cultivars and harvest years, storing apples for longer periods of time and at warmer temperatures may be a strategy to increase polyphenol, particularly flavanol, concentrations in hard cider.


2019 ◽  
Vol 60 (6) ◽  
pp. 925-934 ◽  
Author(s):  
Ewa Dziedzic ◽  
Jan Błaszczyk

Abstract Fruits of the sweet cherry cultivar ‘Regina’ collected from trees growing on seven rootstocks were stored in a cold room at 2 °C with a normal (NA) and controlled atmosphere (15% and 20% CO2 and 5% O2—CA1 and CA2) for 2 weeks. The rootstocks on which the trees grew and the storage conditions significantly affected all fruit parameters tested during both years of the experiment. Fruit from Damil rootstock exhibited higher mean firmness than fruit from Colt rootstock. The effect of rootstocks on the value of soluble solids content (SSC) varied, wherein the fruits from Tabel Edabriz and Damil were characterized by high SSC mean content. The organic acids content (TA) was significantly lower after storage than during harvest time. Fruits from Tabel Edabriz trees were characterized by faster ripening, as was evident by the higher SSC to TA ratio. The amount of mass lost depended significantly and only on the storage conditions—sweet cherries from both CA combinations had the lowest mass losses. The percentage of fruits showing disease symptoms was largely dependent on the weather conditions in the orchard the year before the fruit harvest, as well as atmosphere composition and RH during fruit storage. Cold storage conditions with a high (20%) CO2 content are recommended for the short-term storage of sweet cherry fruits because they preserve fruit quality parameters: a low decrease in firmness, maintenance of a high SSC/TA ratio, a low percent of fungal infections, and good preservation of green color in the peduncle.


2021 ◽  
Vol 49 (11) ◽  
pp. 030006052110568
Author(s):  
Masaki Yamaguchi ◽  
Tomonobu Koizumi ◽  
Mitsutoshi Sugano

Objective To quantitate plasma interleukin-6 (IL-6) levels in healthy individuals and to clarify how these levels are affected by blood sample handling procedures during short-term storage. Methods Ethylenediaminetetraacetic acid (EDTA)-treated plasma samples were simultaneously collected from 14 healthy individuals and stored on ice prior to analysis of the IL-6 levels. White blood cells (WBCs), red blood cells, and platelets were counted immediately after blood collection. IL-6 levels were analyzed every 30 minutes using a commercial electrochemiluminescence immunoassay. Results Correlation coefficients between plasma IL-6 levels and WBC counts ranged between 0.605 and 0.554, higher than those for other cell types. The lowest IL-6 value in healthy individuals was estimated at 0.04 pg/mL and the mean values remained under 2 pg/mL over time. Conclusion Analysis of IL-6 levels in EDTA-treated plasma samples centrifuged within 1 hour and stored on ice can be performed within 90 minutes of short-term storage if the analytical method has a sensitivity in the range of 10 fg/mL.


HortScience ◽  
1992 ◽  
Vol 27 (7) ◽  
pp. 798-800 ◽  
Author(s):  
M.P. Kaczperski ◽  
A.M. Armitage

The effects of storage conditions before transplanting were examined for Petunia × hybrida Vilm. `Supercascade Lilac', viola × wittrockiana Gams `Universal Beaconsfield', and Salvia splendens F. Sellow ex Roem. & Schult `Red Hot Sally'. Plug grown seedlings were stored for 0, 7, 14, or 21 days at 5 or 10C and with continuous irradiance levels from incandescent bulbs at 0, 2, or 12 μmol·m-2·s-1. A second group was stored at 18C with irradiance from fluorescent bulbs at 105 μmol·m-2·s-1 and a 16-hour photoperiod for the same durations. Temperature was more important than irradiance in maintaining a commercially acceptable plant during the storage period. Petunia and pansy could be stored successfully for 21 days at 5 or 10C with no appreciable loss of quality; salvia could be stored for a minimum of 14 days. Seedlings of all species elongated excessively when stored >7 days at 18C and 105 μmol·m-2·s-1 irradiance. After 14 days of storage, petunias stored at 18C flowered sooner than those stored at 5 or 10C but time in a production environment (days to flower - days in storage) was similar for petunias stored at 5 or 18C.


1983 ◽  
Vol 29 (11) ◽  
pp. 1959-1960 ◽  
Author(s):  
C L Winek ◽  
L J Paul

Abstract We examined the effects of time, temperature, and a preservative (sodium fluoride) on ethanol concentrations in stored samples of whole blood from living human subjects. We measured the ethanol in the first, second, seventh, and 14th day of storage, by gas chromatography. Samples were stored at 0-3 degrees C and at 22-29 degrees C, with and without preservative. None of these showed significant gains or losses in concentration. The average differences between ethanol as measured on the day of collection and after storage were all within the range of experimental error of the method (+/- 5%).


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