Neopterin measured in serum and tissue culture supernates by a competitive enzyme-linked immunosorbant assay.

1989 ◽  
Vol 35 (7) ◽  
pp. 1467-1471 ◽  
Author(s):  
M Barak ◽  
D Merzbach ◽  
N Gruener
Keyword(s):  
Tissue Culture ◽  
Correlation Coefficient ◽  
Cell Cultures ◽  
Solid Phase ◽  
Activated Macrophages ◽  
Immunosorbant Assay ◽  
Analytical Recovery ◽  
Enzyme Linked Immunosorbant Assay

Abstract In this solid-phase competitive enzyme-linked immunosorbant assay for neopterin (a product of activated macrophages) in serum or supernates of cell cultures, we incubate antiserum to neopterin with standards or samples in the presence of solid-phase-bound conjugate of carrier-neopterin. Incubation with second antibody labeled with peroxidase then follows, before reaction with substrate. Analytical recovery, precision, and sensitivity of this method are similar to those of other immunoassays. The assay range is between 0.4 and 100 micrograms of neopterin per liter. Results obtained by this method compared with those from a conventional radioimmunoassay gave a correlation coefficient of 0.974.

Solid-phase enzyme immunoassay of urokinase using monoclonal antibodies

1983 ◽  
Vol 3 (4) ◽  
pp. 381-388 ◽  
Author(s):  
P. Hérion ◽  
D. Portetelle ◽  
J. -D. Franssen ◽  
J. Urbain ◽  
A. Bollen
Keyword(s):  
Monoclonal Antibodies ◽  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Biological Fluids ◽  
Immunosorbant Assay ◽  
Rapid Procedure ◽  
Preliminary Purification ◽  
Enzyme Linked Immunosorbant Assay

Using two monoclonal antibodies directed against urokinase, we have developed a micro enzyme-linked immunosorbant assay (ELISA) to detect and measure urokinase in biological fluids. The system presents the following characteristics: simple and rapid procedure, reproducibility, sensitivity (urokinase levels down to 1 ng/ml) and evaluation of the enzyme in biological fluids such as urine, pleural elfusions, and ascitic fluids without preliminary purification.

Measurement of ferritin in serum by an indirect competitive enzyme-linked immunosorbant assay.

1988 ◽  
Vol 34 (4) ◽  
pp. 661-664 ◽  
Author(s):  
R J Polson ◽  
J G Kenna ◽  
I P Shears ◽  
A Bomford ◽  
R Williams
Keyword(s):  
Detection Limit ◽  
Correlation Coefficient ◽  
Serum Samples ◽  
Immunosorbant Assay ◽  
Comparison Of Results ◽  
Microtiter Plates ◽  
Enzyme Conjugate ◽  
Enzyme Linked Immunosorbant Assay

Abstract This indirect competitive enzyme-linked immunosorbant assay (ELISA) for ferritin, unlike other currently available ELISAS, does not require use of an anti-ferritin antibody-enzyme conjugate. Designed for use on microtiter plates, the method has a precision and sensitivity similar to those of other immunoassays. The detection limit is 20 pg of ferritin per test (corresponding to 2.0 micrograms/L in serum samples). Comparison of results obtained on serum from 57 patients by this method with those from a conventional radioimmunoassay gave a correlation coefficient of 0.92.

Evaluation of a new commercial solid-phase direct radioimmunoassay for unconjugated estriol in pregnancy plasma.

1982 ◽  
Vol 28 (10) ◽  
pp. 2103-2105 ◽  
Author(s):  
J T France ◽  
B S Knox ◽  
P R Fisher
Keyword(s):  
Correlation Coefficient ◽  
Solid Phase ◽  
Regression Line ◽  
Assay Method ◽  
Analytical Recovery ◽  
Line Equation ◽  
The Mean ◽  
Assay Precision ◽  
Unconjugated Estriol ◽  
In Pregnancy

Abstract We evaluated a new radioimmunoassay kit for unconjugated estriol in pregnancy plasma. The overall mean intra-assay precision (CV), as determined from replicate analyses of three plasma pools with different estriol concentrations, was 5%; the overall mean inter-assay precision was 7.7%. The assay system had acceptable linearity, with a correlation coefficient of 0.97 between results for 24 plasma samples assayed at 10 and 20 microL. Analytical recovery of estriol added to plasma to give three concentrations averaged 98.6%. Estriol values were generally higher with the kit than with our conventional charcoal-separation RIA method. The regression line equation was y = 1.11x + 1.0, the correlation coefficient 0.97. In plasma from 28 normal pregnant women, sampled serially during the last trimester, the mean unconjugated estriol concentration in plasma increased steadily from 29 nmol/L at 28 weeks of gestation to 42 nmol/L at 34 weeks, and then more rapidly to 93 nmol/L at term. This kit provides a rapid, technically simple, and reliable assay method, offering advantages to clinical laboratories with a high estriol workload.

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

NUTRITION OF ANIMAL CELLS IN TISSUE CULTURE: VII. USE OF REPLICATE CELL CULTURES IN THE EVALUATION OF SYNTHETIC MEDIA

1954 ◽  
Vol 32 (1) ◽  
pp. 306-318
Author(s):  
Raymond C. Parker ◽  
George M. Healy ◽  
Dorothy C. Fisher
Keyword(s):  
Tissue Culture ◽  
Cell Cultures ◽  
Subsequent Treatment ◽  
Relative Toxicity ◽  
Animal Cells ◽  
Washed Cell ◽  
Mouse Cells ◽  
Synthetic Media ◽  
Simple Screening ◽  
Natural Media

The replicate culture assay procedures of Earle and his associates have been adapted for use in evaluating the effectiveness of synthetic media. For this purpose, use has also been made of Earle's L strain mouse cells. Washed and continuously stirred suspensions of these or similar strains of cells may be dispensed, with reasonable assurance of uniformity, into a series of replicate cultures, the number depending on the volume of the suspension and the capacity and effectiveness of the stirring and dispensing unit. For use with synthetic media, the original procedures for the preparation and care of the replicate cultures and for their subsequent treatment for the counting of isolated, stained nuclei have been modified considerably. This paper describes the procedures that were finally adopted and also describes a relatively simple screening procedure in which washed cell suspensions may be used to advantage in making preliminary assays of synthetic media and in testing the relative toxicity or growth stimulating effects of substances added to, or derived from, natural media.

NUTRITION OF ANIMAL CELLS IN TISSUE CULTURE: VII. USE OF REPLICATE CELL CULTURES IN THE EVALUATION OF SYNTHETIC MEDIA

1954 ◽  
Vol 32 (3) ◽  
pp. 306-318 ◽  
Author(s):  
Raymond C. Parker ◽  
George M. Healy ◽  
Dorothy C. Fisher
Keyword(s):  
Tissue Culture ◽  
Cell Cultures ◽  
Subsequent Treatment ◽  
Relative Toxicity ◽  
Animal Cells ◽  
Washed Cell ◽  
Mouse Cells ◽  
Synthetic Media ◽  
Simple Screening ◽  
Natural Media

The replicate culture assay procedures of Earle and his associates have been adapted for use in evaluating the effectiveness of synthetic media. For this purpose, use has also been made of Earle's L strain mouse cells. Washed and continuously stirred suspensions of these or similar strains of cells may be dispensed, with reasonable assurance of uniformity, into a series of replicate cultures, the number depending on the volume of the suspension and the capacity and effectiveness of the stirring and dispensing unit. For use with synthetic media, the original procedures for the preparation and care of the replicate cultures and for their subsequent treatment for the counting of isolated, stained nuclei have been modified considerably. This paper describes the procedures that were finally adopted and also describes a relatively simple screening procedure in which washed cell suspensions may be used to advantage in making preliminary assays of synthetic media and in testing the relative toxicity or growth stimulating effects of substances added to, or derived from, natural media.

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