Effects of Serum Bilirubin on Determination of Uric Acid by the Uricase-Peroxidase Coupled Reaction

1992 ◽  
Vol 38 (7) ◽  
pp. 1350-1352 ◽  
Author(s):  
Y Aoki ◽  
H Ihara ◽  
H Nakamura ◽  
T Aoki ◽  
M Yoshida
Keyword(s):  
Uric Acid ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Serum Bilirubin ◽  
Ammonium Sulfate Precipitation ◽  
Human Bile ◽  
Uric Acid Determination ◽  
Coupled Reaction ◽  
Acid Determination

Abstract We examined the possible effect of different bilirubin species, including unconjugated (Bu), sugar-conjugated (Bc), and authentic delta bilirubin (Bd) isolated from serum, on the direct enzymatic measurement of uric acid by using the uricase (EC 1.7.3.3) and peroxidase (EC 1.11.1.7) coupled reaction. Bc, isolated from human bile, produced the most interference with uric acid determination. Although the presence of human serum albumin reduced interference by Bu and Bc, albumin-bound Bc complex still produced greater interference than Bu. Interference with the peroxidase reaction by Bc covalently bound to serum albumin (Bd) was less than that by Bu. We examined this phenomenon by using serum-isolated Bd obtained by ammonium sulfate precipitation and ultrafiltration and by using commercially available ditaurobilirubin (DTB) and chemically synthesized Bd (via Woodward's reagent, WBd) as surrogates for bile-isolated Bc and natural Bd, respectively. DTB produced the same amount of interference as natural Bc, but the interference produced by DTB in the presence of serum albumin was not the same as that produced by natural Bc with albumin. Our synthetic Bd appears to be a reliable surrogate for natural Bd in testing for bilirubin interference.

Improved automated kinetic determination of uric acid in serum by use of uricase/catalase/aldehyde dehydrogenase.

1979 ◽  
Vol 25 (4) ◽  
pp. 619-621 ◽  
Author(s):  
K Bartl ◽  
M Brandhuber ◽  
J Ziegenhorn
Keyword(s):  
Uric Acid ◽  
Aldehyde Dehydrogenase ◽  
Alcohol Dehydrogenase Isoenzymes ◽  
Kinetic Determination ◽  
Enzymatic Determination ◽  
Other Substances ◽  
Inhibitor System ◽  
Uric Acid Determination ◽  
Acid Determination

Abstract The enzymatic determination of serum uric acid by use of uricase, catalase, and aldehyde dehydrogenase according to Haeckel [J. Clin. Chem. Clin Biochem. 14, 101 (1976)] showed interferences from ethanol-converting enzymes, which are present in some patients' sera. We have identified these enzymes as alcohol dehydrogenase isoenzymes. Among other substances, a mixture of pyrazole and oxalate can be used to eliminate these interferences. This inhibitor system gives good results when used in the automated kinetic uric acid determination, as is shown by a comparison with the manual assay for uric acid according to Kageyama [Clin. Chim. Acta 31, 421 (1971)].

Spectrofluorimetric determination of uric acid based on its activation of catalytic oxidation of pyronine Y

2008 ◽  
Vol 62 (3) ◽  
Author(s):  
Suling Feng ◽  
Xueping Liu
Keyword(s):  
Uric Acid ◽  
Buffer Solution ◽  
Activation Effect ◽  
Buffer Solutions ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Uric Acid Determination ◽  
Catalyzed Oxidation ◽  
Acid Determination

AbstractA novel kinetic spectrofluorimetric method for the determination of uric acid based on the activation effect of uric acid on the Cu(II) ion catalyzed oxidation of pyronine Y by hydrogen peroxide was developed. The influence of different buffer solutions was tested and the Britton-Robinson buffer solution with pH 2.2 was found to be the optimum. The detection limit and the linear range for uric acid are 0.09 μg mL−1 and 0.3–3.0 μg mL−1, respectively. The RSD for eleven determinations of 1.6 μg mL−1 uric acid was 1.6 %. Satisfactory results were obtained when using this method of uric acid determination in human urine.

A bimetallic nanoparticle/graphene oxide/thionine composite-modified glassy carbon electrode used as a facile ratiometric electrochemical sensor for sensitive uric acid determination

2018 ◽  
Vol 42 (18) ◽  
pp. 14796-14804 ◽  
Author(s):  
Xiaohui Gao ◽  
Rijun Gui ◽  
Kendrick Qizhou Xu ◽  
Huijun Guo ◽  
Hui Jin ◽  
...  
Keyword(s):  
Uric Acid ◽  
Graphene Oxide ◽  
Electrochemical Sensor ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
Bimetallic Nanoparticle ◽  
Sensitive Determination ◽  
Uric Acid Determination ◽  
Acid Determination

A novel and facile ratiometric electrochemical sensor was developed for sensitive determination of uric acid.

scholarly journals Modification of Electrode Using Arrowroot Starch Membrane for Uric Acid Determination

Molekul ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. 186
Author(s):  
Elvian Eka Krisnaniningrum ◽  
Ani Mulyasuryani ◽  
Hermin Sulistyarti
Keyword(s):  
Uric Acid ◽  
Detection Limit ◽  
Sodium Tripolyphosphate ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
Concentration Sensitivity ◽  
Uric Acid Determination ◽  
Arrowroot Starch ◽  
Acid Determination

Arrowroot starch membrane-modified glassy carbon electrode were constructed for the determination of uric acid. The membrane consist of arrowroot starch, polyvinyl alcohol, uric acid, and crosslinker. The crosslinker used was sodium tripolyphosphate, citric acid, and glutaraldehyde. Carbon material was added to increase the sensitivity. The composition of membrane influences the electrodes sensitivity. The best composition of arrowroot starch membrane is UA1 using 0.1% uric acid in membrane and STPP as crosslinker. The linearity concentration, sensitivity, and detection limit were 100-500 µM, 0.0509 A/M and 76 µM, respectively.

scholarly journals Simultaneous Determination of Human Serum Albumin and Low-Molecular-Weight Thiols after Derivatization with Monobromobimane

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3321
Author(s):  
Katarzyna Kurpet ◽  
Rafał Głowacki ◽  
Grażyna Chwatko
Keyword(s):  
Molecular Weight ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Simultaneous Determination ◽  
Reversed Phase ◽  
Fluorescence Labeling ◽  
Detector Response ◽  
Low Molecular Weight

Biothiols are extremely powerful antioxidants that protect cells against the effects of oxidative stress. They are also considered relevant disease biomarkers, specifically risk factors for cardiovascular disease. In this paper, a new procedure for the simultaneous determination of human serum albumin and low-molecular-weight thiols in plasma is described. The method is based on the pre-column derivatization of analytes with a thiol-specific fluorescence labeling reagent, monobromobimane, followed by separation and quantification through reversed-phase high-performance liquid chromatography with fluorescence detection (excitation, 378 nm; emission, 492 nm). Prior to the derivatization step, the oxidized thiols are converted to their reduced forms by reductive cleavage with sodium borohydride. Linearity in the detector response for total thiols was observed in the following ranges: 1.76–30.0 mg mL−1 for human serum albumin, 0.29–5.0 nmol mL−1 for α-lipoic acid, 1.16–35 nmol mL−1 for glutathione, 9.83–450.0 nmol mL−1 for cysteine, 0.55–40.0 nmol mL−1 for homocysteine, 0.34–50.0 nmol mL−1 for N-acetyl-L-cysteine, and 1.45–45.0 nmol mL−1 for cysteinylglycine. Recovery values of 85.16–119.48% were recorded for all the analytes. The developed method is sensitive, repeatable, and linear within the expected ranges of total thiols. The devised procedure can be applied to plasma samples to monitor biochemical processes in various pathophysiological states.

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